This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGGTAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 91.82 s (26 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 204,805 (5.9%) Reads that were too short: 21,817 (0.6%) Reads written (passing filters): 182,988 (5.3%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 48,809,518 bp (4.7%) === Adapter 1 === Sequence: AGGTAA; Type: regular 5'; Length: 6; Trimmed: 204805 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 885 846.0 0 885 7 76099 846.0 0 76099 8 619 846.0 0 619 9 63443 846.0 0 63443 10 108 846.0 0 108 11 27 846.0 0 27 12 29 846.0 0 29 13 34 846.0 0 34 14 28 846.0 0 28 15 22 846.0 0 22 16 30 846.0 0 30 17 33 846.0 0 33 18 34 846.0 0 34 19 24 846.0 0 24 20 28 846.0 0 28 21 32 846.0 0 32 22 49 846.0 0 49 23 35 846.0 0 35 24 27 846.0 0 27 25 32 846.0 0 32 26 40 846.0 0 40 27 28 846.0 0 28 28 40 846.0 0 40 29 124 846.0 0 124 30 83 846.0 0 83 31 134 846.0 0 134 32 112 846.0 0 112 33 114 846.0 0 114 34 138 846.0 0 138 35 6255 846.0 0 6255 36 36 846.0 0 36 37 50 846.0 0 50 38 27 846.0 0 27 39 44 846.0 0 44 40 35 846.0 0 35 41 35 846.0 0 35 42 39 846.0 0 39 43 27 846.0 0 27 44 32 846.0 0 32 45 35 846.0 0 35 46 30 846.0 0 30 47 26 846.0 0 26 48 28 846.0 0 28 49 33 846.0 0 33 50 61 846.0 0 61 51 69 846.0 0 69 52 44 846.0 0 44 53 44 846.0 0 44 54 42 846.0 0 42 55 317 846.0 0 317 56 74 846.0 0 74 57 23 846.0 0 23 58 43 846.0 0 43 59 32 846.0 0 32 60 149 846.0 0 149 61 32 846.0 0 32 62 48 846.0 0 48 63 58 846.0 0 58 64 562 846.0 0 562 65 107 846.0 0 107 66 31 846.0 0 31 67 96 846.0 0 96 68 342 846.0 0 342 69 68 846.0 0 68 70 279 846.0 0 279 71 29 846.0 0 29 72 41 846.0 0 41 73 33 846.0 0 33 74 37 846.0 0 37 75 41 846.0 0 41 76 24 846.0 0 24 77 26 846.0 0 26 78 24 846.0 0 24 79 44 846.0 0 44 80 78 846.0 0 78 81 77 846.0 0 77 82 81 846.0 0 81 83 54 846.0 0 54 84 61 846.0 0 61 85 75 846.0 0 75 86 93 846.0 0 93 87 158 846.0 0 158 88 115 846.0 0 115 89 63 846.0 0 63 90 45 846.0 0 45 91 32 846.0 0 32 92 65 846.0 0 65 93 99 846.0 0 99 94 157 846.0 0 157 95 786 846.0 0 786 96 426 846.0 0 426 97 155 846.0 0 155 98 142 846.0 0 142 99 184 846.0 0 184 100 295 846.0 0 295 101 495 846.0 0 495 102 275 846.0 0 275 103 237 846.0 0 237 104 142 846.0 0 142 105 47 846.0 0 47 106 31 846.0 0 31 107 47 846.0 0 47 108 58 846.0 0 58 109 70 846.0 0 70 110 118 846.0 0 118 111 99 846.0 0 99 112 90 846.0 0 90 113 114 846.0 0 114 114 77 846.0 0 77 115 83 846.0 0 83 116 446 846.0 0 446 117 393 846.0 0 393 118 318 846.0 0 318 119 131 846.0 0 131 120 156 846.0 0 156 121 460 846.0 0 460 122 169 846.0 0 169 123 167 846.0 0 167 124 136 846.0 0 136 125 253 846.0 0 253 126 308 846.0 0 308 127 214 846.0 0 214 128 115 846.0 0 115 129 144 846.0 0 144 130 277 846.0 0 277 131 251 846.0 0 251 132 414 846.0 0 414 133 637 846.0 0 637 134 358 846.0 0 358 135 546 846.0 0 546 136 327 846.0 0 327 137 369 846.0 0 369 138 279 846.0 0 279 139 423 846.0 0 423 140 308 846.0 0 308 141 184 846.0 0 184 142 159 846.0 0 159 143 134 846.0 0 134 144 156 846.0 0 156 145 207 846.0 0 207 146 286 846.0 0 286 147 222 846.0 0 222 148 149 846.0 0 149 149 144 846.0 0 144 150 125 846.0 0 125 151 153 846.0 0 153 152 148 846.0 0 148 153 178 846.0 0 178 154 177 846.0 0 177 155 315 846.0 0 315 156 343 846.0 0 343 157 379 846.0 0 379 158 262 846.0 0 262 159 213 846.0 0 213 160 183 846.0 0 183 161 180 846.0 0 180 162 149 846.0 0 149 163 103 846.0 0 103 164 156 846.0 0 156 165 172 846.0 0 172 166 206 846.0 0 206 167 313 846.0 0 313 168 235 846.0 0 235 169 175 846.0 0 175 170 216 846.0 0 216 171 175 846.0 0 175 172 194 846.0 0 194 173 202 846.0 0 202 174 264 846.0 0 264 175 858 846.0 0 858 176 371 846.0 0 371 177 341 846.0 0 341 178 234 846.0 0 234 179 282 846.0 0 282 180 950 846.0 0 950 181 637 846.0 0 637 182 628 846.0 0 628 183 347 846.0 0 347 184 285 846.0 0 285 185 330 846.0 0 330 186 283 846.0 0 283 187 292 846.0 0 292 188 194 846.0 0 194 189 128 846.0 0 128 190 165 846.0 0 165 191 190 846.0 0 190 192 279 846.0 0 279 193 395 846.0 0 395 194 651 846.0 0 651 195 487 846.0 0 487 196 449 846.0 0 449 197 368 846.0 0 368 198 394 846.0 0 394 199 355 846.0 0 355 200 899 846.0 0 899 201 533 846.0 0 533 202 727 846.0 0 727 203 968 846.0 0 968 204 185 846.0 0 185 205 208 846.0 0 208 206 279 846.0 0 279 207 240 846.0 0 240 208 192 846.0 0 192 209 147 846.0 0 147 210 148 846.0 0 148 211 188 846.0 0 188 212 254 846.0 0 254 213 275 846.0 0 275 214 264 846.0 0 264 215 187 846.0 0 187 216 156 846.0 0 156 217 176 846.0 0 176 218 228 846.0 0 228 219 270 846.0 0 270 220 189 846.0 0 189 221 171 846.0 0 171 222 156 846.0 0 156 223 184 846.0 0 184 224 215 846.0 0 215 225 158 846.0 0 158 226 196 846.0 0 196 227 166 846.0 0 166 228 262 846.0 0 262 229 220 846.0 0 220 230 168 846.0 0 168 231 143 846.0 0 143 232 126 846.0 0 126 233 159 846.0 0 159 234 141 846.0 0 141 235 185 846.0 0 185 236 202 846.0 0 202 237 194 846.0 0 194 238 212 846.0 0 212 239 265 846.0 0 265 240 258 846.0 0 258 241 141 846.0 0 141 242 182 846.0 0 182 243 129 846.0 0 129 244 137 846.0 0 137 245 142 846.0 0 142 246 193 846.0 0 193 247 284 846.0 0 284 248 227 846.0 0 227 249 656 846.0 0 656 250 78 846.0 0 78 251 82 846.0 0 82 252 113 846.0 0 113 253 190 846.0 0 190 254 178 846.0 0 178 255 212 846.0 0 212 256 219 846.0 0 219 257 209 846.0 0 209 258 321 846.0 0 321 259 277 846.0 0 277 260 379 846.0 0 379 261 113 846.0 0 113 262 175 846.0 0 175 263 273 846.0 0 273 264 216 846.0 0 216 265 210 846.0 0 210 266 252 846.0 0 252 267 319 846.0 0 319 268 237 846.0 0 237 269 122 846.0 0 122 270 60 846.0 0 60 271 96 846.0 0 96 272 152 846.0 0 152 273 158 846.0 0 158 274 288 846.0 0 288 275 207 846.0 0 207 276 200 846.0 0 200 277 195 846.0 0 195 278 450 846.0 0 450 279 183 846.0 0 183 280 168 846.0 0 168 281 90 846.0 0 90 282 86 846.0 0 86 283 97 846.0 0 97 284 102 846.0 0 102 285 120 846.0 0 120 286 194 846.0 0 194 287 235 846.0 0 235 288 172 846.0 0 172 289 91 846.0 0 91 290 142 846.0 0 142 291 175 846.0 0 175 292 534 846.0 0 534 293 358 846.0 0 358 294 273 846.0 0 273 295 186 846.0 0 186 296 114 846.0 0 114 297 166 846.0 0 166 298 148 846.0 0 148 299 212 846.0 0 212 300 212 846.0 0 212 301 625 846.0 0 625 Finished in 8.73 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 182,988 Reads with adapters: 71,491 (39.1%) Reads that were too short: 27 (0.0%) Reads written (passing filters): 71,464 (39.1%) Total basepairs processed: 48,809,518 bp Total written (filtered): 19,572,583 bp (40.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 71491 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 1 1 18 5 0.0 3 0 0 5 19 230 0.0 3 0 217 11 2 20 70909 0.0 4 70050 809 41 5 4 21 185 0.0 4 30 153 2 22 10 0.0 4 1 0 9 23 37 0.0 4 2 0 0 32 3 24 3 0.0 4 0 0 0 0 3 25 1 0.0 4 0 0 1 36 1 0.0 4 0 0 0 0 1 40 4 0.0 4 0 1 0 0 3 42 1 0.0 4 0 0 0 0 1 44 2 0.0 4 0 0 0 0 2 47 1 0.0 4 0 0 0 0 1 49 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 0 0 0 1 92 2 0.0 4 0 0 0 0 2 98 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 110 1 0.0 4 0 0 0 0 1 111 1 0.0 4 0 0 0 0 1 112 4 0.0 4 0 0 0 4 126 1 0.0 4 0 0 0 0 1 127 54 0.0 4 0 0 0 0 54 128 16 0.0 4 0 0 0 0 16 133 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 0 1 142 1 0.0 4 0 0 0 0 1 147 1 0.0 4 0 0 0 0 1 149 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 238 2 0.0 4 0 0 0 0 2 264 1 0.0 4 0 0 0 0 1 282 2 0.0 4 0 0 0 0 2 290 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.44 s (34 us/read; 1.76 M reads/minute). === Summary === Total reads processed: 71,464 Reads with adapters: 2,267 (3.2%) Reads written (passing filters): 71,464 (100.0%) Total basepairs processed: 19,572,583 bp Total written (filtered): 19,232,902 bp (98.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2267 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 14.2% C: 3.6% G: 43.7% T: 38.3% none/other: 0.1% Overview of removed sequences length count expect max.err error counts 3 17 1116.6 0 17 4 4 279.2 0 4 5 3 69.8 0 3 10 4 0.1 1 0 4 11 986 0.0 1 5 981 12 20 0.0 1 0 20 13 3 0.0 1 0 3 14 1 0.0 1 0 1 152 4 0.0 1 1 3 234 40 0.0 1 37 3 236 1 0.0 1 1 244 1 0.0 1 1 253 1 0.0 1 1 257 2 0.0 1 2 259 1 0.0 1 1 260 3 0.0 1 3 261 248 0.0 1 235 13 262 4 0.0 1 4 263 5 0.0 1 4 1 264 3 0.0 1 3 269 1 0.0 1 1 270 3 0.0 1 3 271 861 0.0 1 840 21 272 50 0.0 1 48 2 273 1 0.0 1 1