This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GATATT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 91.27 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 79,679 (2.3%) Reads that were too short: 25,993 (0.8%) Reads written (passing filters): 53,686 (1.5%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 11,285,752 bp (1.1%) === Adapter 1 === Sequence: GATATT; Type: regular 5'; Length: 6; Trimmed: 79679 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 144 846.0 0 144 7 3242 846.0 0 3242 8 215 846.0 0 215 9 6866 846.0 0 6866 10 97 846.0 0 97 11 90 846.0 0 90 12 71 846.0 0 71 13 79 846.0 0 79 14 79 846.0 0 79 15 74 846.0 0 74 16 106 846.0 0 106 17 80 846.0 0 80 18 87 846.0 0 87 19 84 846.0 0 84 20 101 846.0 0 101 21 120 846.0 0 120 22 129 846.0 0 129 23 81 846.0 0 81 24 92 846.0 0 92 25 94 846.0 0 94 26 113 846.0 0 113 27 119 846.0 0 119 28 80 846.0 0 80 29 93 846.0 0 93 30 89 846.0 0 89 31 105 846.0 0 105 32 100 846.0 0 100 33 102 846.0 0 102 34 144 846.0 0 144 35 947 846.0 0 947 36 363 846.0 0 363 37 549 846.0 0 549 38 101 846.0 0 101 39 79 846.0 0 79 40 101 846.0 0 101 41 101 846.0 0 101 42 643 846.0 0 643 43 131 846.0 0 131 44 97 846.0 0 97 45 90 846.0 0 90 46 78 846.0 0 78 47 64 846.0 0 64 48 83 846.0 0 83 49 79 846.0 0 79 50 73 846.0 0 73 51 83 846.0 0 83 52 85 846.0 0 85 53 73 846.0 0 73 54 66 846.0 0 66 55 71 846.0 0 71 56 79 846.0 0 79 57 65 846.0 0 65 58 84 846.0 0 84 59 100 846.0 0 100 60 115 846.0 0 115 61 83 846.0 0 83 62 72 846.0 0 72 63 101 846.0 0 101 64 319 846.0 0 319 65 77 846.0 0 77 66 78 846.0 0 78 67 83 846.0 0 83 68 103 846.0 0 103 69 901 846.0 0 901 70 292 846.0 0 292 71 308 846.0 0 308 72 74 846.0 0 74 73 74 846.0 0 74 74 103 846.0 0 103 75 89 846.0 0 89 76 173 846.0 0 173 77 153 846.0 0 153 78 150 846.0 0 150 79 148 846.0 0 148 80 378 846.0 0 378 81 211 846.0 0 211 82 221 846.0 0 221 83 175 846.0 0 175 84 133 846.0 0 133 85 128 846.0 0 128 86 142 846.0 0 142 87 208 846.0 0 208 88 294 846.0 0 294 89 820 846.0 0 820 90 511 846.0 0 511 91 188 846.0 0 188 92 147 846.0 0 147 93 205 846.0 0 205 94 256 846.0 0 256 95 248 846.0 0 248 96 578 846.0 0 578 97 806 846.0 0 806 98 980 846.0 0 980 99 1551 846.0 0 1551 100 2696 846.0 0 2696 101 453 846.0 0 453 102 185 846.0 0 185 103 148 846.0 0 148 104 118 846.0 0 118 105 304 846.0 0 304 106 170 846.0 0 170 107 249 846.0 0 249 108 177 846.0 0 177 109 135 846.0 0 135 110 425 846.0 0 425 111 601 846.0 0 601 112 565 846.0 0 565 113 238 846.0 0 238 114 203 846.0 0 203 115 149 846.0 0 149 116 110 846.0 0 110 117 138 846.0 0 138 118 153 846.0 0 153 119 200 846.0 0 200 120 219 846.0 0 219 121 426 846.0 0 426 122 490 846.0 0 490 123 287 846.0 0 287 124 175 846.0 0 175 125 177 846.0 0 177 126 194 846.0 0 194 127 267 846.0 0 267 128 226 846.0 0 226 129 224 846.0 0 224 130 186 846.0 0 186 131 271 846.0 0 271 132 229 846.0 0 229 133 225 846.0 0 225 134 234 846.0 0 234 135 185 846.0 0 185 136 160 846.0 0 160 137 176 846.0 0 176 138 158 846.0 0 158 139 271 846.0 0 271 140 293 846.0 0 293 141 559 846.0 0 559 142 193 846.0 0 193 143 319 846.0 0 319 144 168 846.0 0 168 145 155 846.0 0 155 146 324 846.0 0 324 147 249 846.0 0 249 148 217 846.0 0 217 149 185 846.0 0 185 150 152 846.0 0 152 151 117 846.0 0 117 152 171 846.0 0 171 153 166 846.0 0 166 154 173 846.0 0 173 155 225 846.0 0 225 156 169 846.0 0 169 157 173 846.0 0 173 158 161 846.0 0 161 159 396 846.0 0 396 160 230 846.0 0 230 161 266 846.0 0 266 162 177 846.0 0 177 163 136 846.0 0 136 164 148 846.0 0 148 165 159 846.0 0 159 166 158 846.0 0 158 167 176 846.0 0 176 168 274 846.0 0 274 169 166 846.0 0 166 170 159 846.0 0 159 171 109 846.0 0 109 172 147 846.0 0 147 173 202 846.0 0 202 174 183 846.0 0 183 175 186 846.0 0 186 176 146 846.0 0 146 177 192 846.0 0 192 178 129 846.0 0 129 179 154 846.0 0 154 180 131 846.0 0 131 181 168 846.0 0 168 182 187 846.0 0 187 183 161 846.0 0 161 184 193 846.0 0 193 185 228 846.0 0 228 186 167 846.0 0 167 187 168 846.0 0 168 188 141 846.0 0 141 189 158 846.0 0 158 190 189 846.0 0 189 191 192 846.0 0 192 192 145 846.0 0 145 193 188 846.0 0 188 194 261 846.0 0 261 195 344 846.0 0 344 196 190 846.0 0 190 197 405 846.0 0 405 198 126 846.0 0 126 199 179 846.0 0 179 200 320 846.0 0 320 201 349 846.0 0 349 202 425 846.0 0 425 203 272 846.0 0 272 204 234 846.0 0 234 205 226 846.0 0 226 206 540 846.0 0 540 207 732 846.0 0 732 208 530 846.0 0 530 209 444 846.0 0 444 210 266 846.0 0 266 211 240 846.0 0 240 212 213 846.0 0 213 213 203 846.0 0 203 214 233 846.0 0 233 215 175 846.0 0 175 216 179 846.0 0 179 217 151 846.0 0 151 218 168 846.0 0 168 219 167 846.0 0 167 220 249 846.0 0 249 221 294 846.0 0 294 222 341 846.0 0 341 223 250 846.0 0 250 224 169 846.0 0 169 225 189 846.0 0 189 226 148 846.0 0 148 227 147 846.0 0 147 228 154 846.0 0 154 229 234 846.0 0 234 230 243 846.0 0 243 231 282 846.0 0 282 232 261 846.0 0 261 233 271 846.0 0 271 234 236 846.0 0 236 235 266 846.0 0 266 236 410 846.0 0 410 237 478 846.0 0 478 238 250 846.0 0 250 239 394 846.0 0 394 240 334 846.0 0 334 241 845 846.0 0 845 242 827 846.0 0 827 243 298 846.0 0 298 244 177 846.0 0 177 245 151 846.0 0 151 246 105 846.0 0 105 247 109 846.0 0 109 248 228 846.0 0 228 249 338 846.0 0 338 250 538 846.0 0 538 251 191 846.0 0 191 252 168 846.0 0 168 253 144 846.0 0 144 254 177 846.0 0 177 255 190 846.0 0 190 256 197 846.0 0 197 257 156 846.0 0 156 258 183 846.0 0 183 259 332 846.0 0 332 260 770 846.0 0 770 261 849 846.0 0 849 262 570 846.0 0 570 263 169 846.0 0 169 264 164 846.0 0 164 265 138 846.0 0 138 266 95 846.0 0 95 267 103 846.0 0 103 268 152 846.0 0 152 269 172 846.0 0 172 270 240 846.0 0 240 271 182 846.0 0 182 272 204 846.0 0 204 273 172 846.0 0 172 274 199 846.0 0 199 275 326 846.0 0 326 276 154 846.0 0 154 277 136 846.0 0 136 278 142 846.0 0 142 279 133 846.0 0 133 280 156 846.0 0 156 281 226 846.0 0 226 282 301 846.0 0 301 283 321 846.0 0 321 284 275 846.0 0 275 285 124 846.0 0 124 286 100 846.0 0 100 287 123 846.0 0 123 288 156 846.0 0 156 289 357 846.0 0 357 290 186 846.0 0 186 291 150 846.0 0 150 292 177 846.0 0 177 293 141 846.0 0 141 294 186 846.0 0 186 295 385 846.0 0 385 296 206 846.0 0 206 297 185 846.0 0 185 298 163 846.0 0 163 299 165 846.0 0 165 300 233 846.0 0 233 301 255 846.0 0 255 Finished in 2.08 s (39 us/read; 1.55 M reads/minute). === Summary === Total reads processed: 53,686 Reads with adapters: 6,987 (13.0%) Reads that were too short: 20 (0.0%) Reads written (passing filters): 6,967 (13.0%) Total basepairs processed: 11,285,752 bp Total written (filtered): 1,909,537 bp (16.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 6987 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 4 0.0 3 0 0 3 1 16 107 0.0 3 0 101 2 4 17 6767 0.0 3 6545 198 18 6 18 30 0.0 3 3 21 2 4 19 5 0.0 3 0 0 4 1 20 9 0.0 3 0 0 0 9 22 1 0.0 3 0 0 0 1 24 1 0.0 3 0 0 0 1 26 1 0.0 3 0 0 0 1 34 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 68 2 0.0 3 0 0 0 2 70 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 81 4 0.0 3 0 0 0 4 82 1 0.0 3 0 0 0 1 84 2 0.0 3 0 0 1 1 86 2 0.0 3 0 0 0 2 90 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 134 4 0.0 3 0 0 0 4 139 1 0.0 3 0 0 0 1 141 2 0.0 3 0 0 0 2 144 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 146 2 0.0 3 0 0 0 2 147 1 0.0 3 0 0 0 1 148 2 0.0 3 0 0 0 2 155 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 169 5 0.0 3 0 0 0 5 182 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 197 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 248 2 0.0 3 0 0 0 2 251 1 0.0 3 0 0 0 1 258 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.25 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 6,967 Reads with adapters: 283 (4.1%) Reads written (passing filters): 6,967 (100.0%) Total basepairs processed: 1,909,537 bp Total written (filtered): 1,908,681 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 283 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 41.0% C: 10.6% G: 37.1% T: 11.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 276 108.9 0 276 4 7 27.2 0 7