This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TATACC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 90.41 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 113,578 (3.3%) Reads that were too short: 42,983 (1.2%) Reads written (passing filters): 70,595 (2.0%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 13,783,052 bp (1.3%) === Adapter 1 === Sequence: TATACC; Type: regular 5'; Length: 6; Trimmed: 113578 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 298 846.0 0 298 7 7306 846.0 0 7306 8 112 846.0 0 112 9 11706 846.0 0 11706 10 31 846.0 0 31 11 15 846.0 0 15 12 11 846.0 0 11 13 19 846.0 0 19 14 22 846.0 0 22 15 12 846.0 0 12 16 27 846.0 0 27 17 15 846.0 0 15 18 13 846.0 0 13 19 15 846.0 0 15 20 21 846.0 0 21 21 22 846.0 0 22 22 23 846.0 0 23 23 25 846.0 0 25 24 20 846.0 0 20 25 11 846.0 0 11 26 14 846.0 0 14 27 14 846.0 0 14 28 11 846.0 0 11 29 20 846.0 0 20 30 15 846.0 0 15 31 22 846.0 0 22 32 19 846.0 0 19 33 19 846.0 0 19 34 18 846.0 0 18 35 46 846.0 0 46 36 14 846.0 0 14 37 13 846.0 0 13 38 20 846.0 0 20 39 12 846.0 0 12 40 15 846.0 0 15 41 17 846.0 0 17 42 14 846.0 0 14 43 21 846.0 0 21 44 22 846.0 0 22 45 21 846.0 0 21 46 17 846.0 0 17 47 12 846.0 0 12 48 17 846.0 0 17 49 30 846.0 0 30 50 1113 846.0 0 1113 51 20 846.0 0 20 52 268 846.0 0 268 53 29 846.0 0 29 54 10 846.0 0 10 55 28 846.0 0 28 56 16 846.0 0 16 57 13 846.0 0 13 58 18 846.0 0 18 59 22 846.0 0 22 60 19 846.0 0 19 61 20 846.0 0 20 62 14 846.0 0 14 63 13 846.0 0 13 64 29 846.0 0 29 65 21 846.0 0 21 66 23 846.0 0 23 67 22 846.0 0 22 68 29 846.0 0 29 69 18 846.0 0 18 70 16 846.0 0 16 71 18 846.0 0 18 72 16 846.0 0 16 73 36 846.0 0 36 74 128 846.0 0 128 75 20 846.0 0 20 76 19 846.0 0 19 77 23 846.0 0 23 78 12 846.0 0 12 79 23 846.0 0 23 80 58 846.0 0 58 81 86 846.0 0 86 82 149 846.0 0 149 83 119 846.0 0 119 84 141 846.0 0 141 85 147 846.0 0 147 86 58 846.0 0 58 87 56 846.0 0 56 88 37 846.0 0 37 89 65 846.0 0 65 90 139 846.0 0 139 91 185 846.0 0 185 92 298 846.0 0 298 93 81 846.0 0 81 94 76 846.0 0 76 95 60 846.0 0 60 96 89 846.0 0 89 97 102 846.0 0 102 98 206 846.0 0 206 99 286 846.0 0 286 100 340 846.0 0 340 101 1139 846.0 0 1139 102 1433 846.0 0 1433 103 659 846.0 0 659 104 156 846.0 0 156 105 244 846.0 0 244 106 273 846.0 0 273 107 180 846.0 0 180 108 206 846.0 0 206 109 94 846.0 0 94 110 153 846.0 0 153 111 367 846.0 0 367 112 318 846.0 0 318 113 260 846.0 0 260 114 187 846.0 0 187 115 392 846.0 0 392 116 582 846.0 0 582 117 316 846.0 0 316 118 256 846.0 0 256 119 204 846.0 0 204 120 127 846.0 0 127 121 111 846.0 0 111 122 191 846.0 0 191 123 646 846.0 0 646 124 707 846.0 0 707 125 476 846.0 0 476 126 163 846.0 0 163 127 103 846.0 0 103 128 83 846.0 0 83 129 141 846.0 0 141 130 621 846.0 0 621 131 1371 846.0 0 1371 132 766 846.0 0 766 133 567 846.0 0 567 134 249 846.0 0 249 135 184 846.0 0 184 136 436 846.0 0 436 137 526 846.0 0 526 138 557 846.0 0 557 139 343 846.0 0 343 140 183 846.0 0 183 141 395 846.0 0 395 142 510 846.0 0 510 143 592 846.0 0 592 144 632 846.0 0 632 145 856 846.0 0 856 146 285 846.0 0 285 147 196 846.0 0 196 148 367 846.0 0 367 149 865 846.0 0 865 150 773 846.0 0 773 151 472 846.0 0 472 152 368 846.0 0 368 153 301 846.0 0 301 154 427 846.0 0 427 155 755 846.0 0 755 156 817 846.0 0 817 157 985 846.0 0 985 158 695 846.0 0 695 159 458 846.0 0 458 160 248 846.0 0 248 161 234 846.0 0 234 162 422 846.0 0 422 163 348 846.0 0 348 164 405 846.0 0 405 165 330 846.0 0 330 166 264 846.0 0 264 167 385 846.0 0 385 168 367 846.0 0 367 169 902 846.0 0 902 170 1895 846.0 0 1895 171 1280 846.0 0 1280 172 577 846.0 0 577 173 150 846.0 0 150 174 116 846.0 0 116 175 209 846.0 0 209 176 333 846.0 0 333 177 739 846.0 0 739 178 1131 846.0 0 1131 179 863 846.0 0 863 180 429 846.0 0 429 181 194 846.0 0 194 182 258 846.0 0 258 183 276 846.0 0 276 184 342 846.0 0 342 185 247 846.0 0 247 186 374 846.0 0 374 187 499 846.0 0 499 188 660 846.0 0 660 189 506 846.0 0 506 190 350 846.0 0 350 191 168 846.0 0 168 192 143 846.0 0 143 193 147 846.0 0 147 194 167 846.0 0 167 195 321 846.0 0 321 196 309 846.0 0 309 197 490 846.0 0 490 198 385 846.0 0 385 199 491 846.0 0 491 200 673 846.0 0 673 201 248 846.0 0 248 202 245 846.0 0 245 203 271 846.0 0 271 204 387 846.0 0 387 205 420 846.0 0 420 206 333 846.0 0 333 207 210 846.0 0 210 208 251 846.0 0 251 209 376 846.0 0 376 210 888 846.0 0 888 211 946 846.0 0 946 212 635 846.0 0 635 213 436 846.0 0 436 214 406 846.0 0 406 215 348 846.0 0 348 216 283 846.0 0 283 217 326 846.0 0 326 218 324 846.0 0 324 219 282 846.0 0 282 220 204 846.0 0 204 221 297 846.0 0 297 222 324 846.0 0 324 223 508 846.0 0 508 224 531 846.0 0 531 225 582 846.0 0 582 226 440 846.0 0 440 227 437 846.0 0 437 228 386 846.0 0 386 229 331 846.0 0 331 230 247 846.0 0 247 231 231 846.0 0 231 232 242 846.0 0 242 233 259 846.0 0 259 234 261 846.0 0 261 235 198 846.0 0 198 236 221 846.0 0 221 237 303 846.0 0 303 238 286 846.0 0 286 239 305 846.0 0 305 240 299 846.0 0 299 241 342 846.0 0 342 242 369 846.0 0 369 243 437 846.0 0 437 244 493 846.0 0 493 245 800 846.0 0 800 246 625 846.0 0 625 247 458 846.0 0 458 248 572 846.0 0 572 249 394 846.0 0 394 250 543 846.0 0 543 251 225 846.0 0 225 252 291 846.0 0 291 253 211 846.0 0 211 254 347 846.0 0 347 255 364 846.0 0 364 256 327 846.0 0 327 257 414 846.0 0 414 258 551 846.0 0 551 259 446 846.0 0 446 260 360 846.0 0 360 261 354 846.0 0 354 262 387 846.0 0 387 263 502 846.0 0 502 264 1082 846.0 0 1082 265 668 846.0 0 668 266 407 846.0 0 407 267 162 846.0 0 162 268 206 846.0 0 206 269 216 846.0 0 216 270 485 846.0 0 485 271 534 846.0 0 534 272 759 846.0 0 759 273 799 846.0 0 799 274 507 846.0 0 507 275 422 846.0 0 422 276 402 846.0 0 402 277 463 846.0 0 463 278 358 846.0 0 358 279 237 846.0 0 237 280 276 846.0 0 276 281 471 846.0 0 471 282 467 846.0 0 467 283 597 846.0 0 597 284 1024 846.0 0 1024 285 1796 846.0 0 1796 286 982 846.0 0 982 287 405 846.0 0 405 288 254 846.0 0 254 289 319 846.0 0 319 290 315 846.0 0 315 291 361 846.0 0 361 292 261 846.0 0 261 293 213 846.0 0 213 294 191 846.0 0 191 295 167 846.0 0 167 296 257 846.0 0 257 297 468 846.0 0 468 298 640 846.0 0 640 299 775 846.0 0 775 300 680 846.0 0 680 301 286 846.0 0 286 Finished in 2.82 s (40 us/read; 1.50 M reads/minute). === Summary === Total reads processed: 70,595 Reads with adapters: 7,501 (10.6%) Reads that were too short: 18 (0.0%) Reads written (passing filters): 7,483 (10.6%) Total basepairs processed: 13,783,052 bp Total written (filtered): 2,043,327 bp (14.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 7501 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 1 0.0 3 0 0 1 19 31 0.0 3 0 31 20 7278 0.0 4 7171 100 6 1 21 15 0.0 4 1 12 0 2 22 2 0.0 4 2 23 94 0.0 4 83 3 0 6 2 43 1 0.0 4 1 46 1 0.0 4 0 0 0 0 1 49 1 0.0 4 0 0 0 0 1 57 1 0.0 4 0 0 0 0 1 59 1 0.0 4 0 0 0 0 1 60 1 0.0 4 0 0 0 0 1 66 1 0.0 4 0 0 0 0 1 67 1 0.0 4 0 0 0 0 1 70 1 0.0 4 0 0 0 0 1 78 2 0.0 4 0 0 0 0 2 79 1 0.0 4 0 0 0 0 1 83 2 0.0 4 0 0 0 0 2 86 1 0.0 4 0 0 0 0 1 93 1 0.0 4 0 0 0 0 1 99 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 0 1 113 1 0.0 4 0 0 0 0 1 122 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 54 0.0 4 0 0 0 0 54 144 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 154 1 0.0 4 0 0 0 0 1 189 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.25 s (33 us/read; 1.80 M reads/minute). === Summary === Total reads processed: 7,483 Reads with adapters: 86 (1.1%) Reads written (passing filters): 7,483 (100.0%) Total basepairs processed: 2,043,327 bp Total written (filtered): 2,038,739 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 86 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 14.0% C: 5.8% G: 79.1% T: 1.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 4 116.9 0 4 10 4 0.0 1 1 3 11 60 0.0 1 1 59 12 3 0.0 1 0 3 210 1 0.0 1 0 1 257 6 0.0 1 6 259 1 0.0 1 1 260 4 0.0 1 4 262 1 0.0 1 1 263 1 0.0 1 1 264 1 0.0 1 1