This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GAAGCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 94.63 s (27 us/read; 2.20 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 256,213 (7.4%) Reads that were too short: 15,369 (0.4%) Reads written (passing filters): 240,844 (7.0%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 54,717,690 bp (5.2%) === Adapter 1 === Sequence: GAAGCT; Type: regular 5'; Length: 6; Trimmed: 256213 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 365 846.0 0 365 7 1275 846.0 0 1275 8 31877 846.0 0 31877 9 19265 846.0 0 19265 10 147 846.0 0 147 11 43 846.0 0 43 12 32 846.0 0 32 13 15 846.0 0 15 14 40 846.0 0 40 15 20 846.0 0 20 16 27 846.0 0 27 17 29 846.0 0 29 18 24 846.0 0 24 19 21 846.0 0 21 20 15 846.0 0 15 21 27 846.0 0 27 22 30 846.0 0 30 23 36 846.0 0 36 24 31 846.0 0 31 25 46 846.0 0 46 26 36 846.0 0 36 27 54 846.0 0 54 28 60 846.0 0 60 29 34 846.0 0 34 30 35 846.0 0 35 31 30 846.0 0 30 32 41 846.0 0 41 33 35 846.0 0 35 34 168 846.0 0 168 35 88 846.0 0 88 36 15 846.0 0 15 37 51 846.0 0 51 38 42 846.0 0 42 39 34 846.0 0 34 40 23 846.0 0 23 41 16 846.0 0 16 42 28 846.0 0 28 43 26 846.0 0 26 44 41 846.0 0 41 45 32 846.0 0 32 46 33 846.0 0 33 47 19 846.0 0 19 48 32 846.0 0 32 49 20 846.0 0 20 50 33 846.0 0 33 51 34 846.0 0 34 52 34 846.0 0 34 53 53 846.0 0 53 54 155 846.0 0 155 55 126 846.0 0 126 56 41 846.0 0 41 57 19 846.0 0 19 58 25 846.0 0 25 59 28 846.0 0 28 60 26 846.0 0 26 61 48 846.0 0 48 62 63 846.0 0 63 63 52 846.0 0 52 64 180 846.0 0 180 65 50 846.0 0 50 66 113 846.0 0 113 67 64 846.0 0 64 68 50 846.0 0 50 69 66 846.0 0 66 70 40 846.0 0 40 71 73 846.0 0 73 72 57 846.0 0 57 73 85 846.0 0 85 74 152 846.0 0 152 75 192 846.0 0 192 76 431 846.0 0 431 77 377 846.0 0 377 78 647 846.0 0 647 79 1302 846.0 0 1302 80 1527 846.0 0 1527 81 19091 846.0 0 19091 82 113845 846.0 0 113845 83 5608 846.0 0 5608 84 168 846.0 0 168 85 189 846.0 0 189 86 236 846.0 0 236 87 366 846.0 0 366 88 100 846.0 0 100 89 79 846.0 0 79 90 91 846.0 0 91 91 87 846.0 0 87 92 62 846.0 0 62 93 159 846.0 0 159 94 111 846.0 0 111 95 60 846.0 0 60 96 78 846.0 0 78 97 98 846.0 0 98 98 54 846.0 0 54 99 117 846.0 0 117 100 96 846.0 0 96 101 41 846.0 0 41 102 245 846.0 0 245 103 45 846.0 0 45 104 51 846.0 0 51 105 46 846.0 0 46 106 63 846.0 0 63 107 71 846.0 0 71 108 106 846.0 0 106 109 241 846.0 0 241 110 111 846.0 0 111 111 128 846.0 0 128 112 172 846.0 0 172 113 95 846.0 0 95 114 115 846.0 0 115 115 136 846.0 0 136 116 118 846.0 0 118 117 188 846.0 0 188 118 205 846.0 0 205 119 932 846.0 0 932 120 358 846.0 0 358 121 419 846.0 0 419 122 4306 846.0 0 4306 123 3083 846.0 0 3083 124 16969 846.0 0 16969 125 173 846.0 0 173 126 124 846.0 0 124 127 79 846.0 0 79 128 112 846.0 0 112 129 97 846.0 0 97 130 98 846.0 0 98 131 210 846.0 0 210 132 73 846.0 0 73 133 110 846.0 0 110 134 105 846.0 0 105 135 99 846.0 0 99 136 79 846.0 0 79 137 131 846.0 0 131 138 119 846.0 0 119 139 96 846.0 0 96 140 99 846.0 0 99 141 121 846.0 0 121 142 124 846.0 0 124 143 161 846.0 0 161 144 906 846.0 0 906 145 482 846.0 0 482 146 507 846.0 0 507 147 355 846.0 0 355 148 95 846.0 0 95 149 126 846.0 0 126 150 91 846.0 0 91 151 209 846.0 0 209 152 153 846.0 0 153 153 124 846.0 0 124 154 143 846.0 0 143 155 111 846.0 0 111 156 101 846.0 0 101 157 99 846.0 0 99 158 97 846.0 0 97 159 116 846.0 0 116 160 135 846.0 0 135 161 87 846.0 0 87 162 90 846.0 0 90 163 92 846.0 0 92 164 92 846.0 0 92 165 85 846.0 0 85 166 79 846.0 0 79 167 81 846.0 0 81 168 107 846.0 0 107 169 152 846.0 0 152 170 96 846.0 0 96 171 241 846.0 0 241 172 88 846.0 0 88 173 107 846.0 0 107 174 103 846.0 0 103 175 97 846.0 0 97 176 126 846.0 0 126 177 122 846.0 0 122 178 92 846.0 0 92 179 93 846.0 0 93 180 87 846.0 0 87 181 90 846.0 0 90 182 666 846.0 0 666 183 282 846.0 0 282 184 352 846.0 0 352 185 95 846.0 0 95 186 87 846.0 0 87 187 70 846.0 0 70 188 124 846.0 0 124 189 141 846.0 0 141 190 142 846.0 0 142 191 80 846.0 0 80 192 88 846.0 0 88 193 91 846.0 0 91 194 120 846.0 0 120 195 80 846.0 0 80 196 178 846.0 0 178 197 203 846.0 0 203 198 76 846.0 0 76 199 70 846.0 0 70 200 78 846.0 0 78 201 114 846.0 0 114 202 138 846.0 0 138 203 81 846.0 0 81 204 70 846.0 0 70 205 212 846.0 0 212 206 276 846.0 0 276 207 114 846.0 0 114 208 79 846.0 0 79 209 105 846.0 0 105 210 127 846.0 0 127 211 125 846.0 0 125 212 114 846.0 0 114 213 124 846.0 0 124 214 107 846.0 0 107 215 107 846.0 0 107 216 103 846.0 0 103 217 95 846.0 0 95 218 93 846.0 0 93 219 105 846.0 0 105 220 107 846.0 0 107 221 188 846.0 0 188 222 151 846.0 0 151 223 95 846.0 0 95 224 97 846.0 0 97 225 96 846.0 0 96 226 95 846.0 0 95 227 79 846.0 0 79 228 81 846.0 0 81 229 73 846.0 0 73 230 81 846.0 0 81 231 101 846.0 0 101 232 85 846.0 0 85 233 123 846.0 0 123 234 125 846.0 0 125 235 65 846.0 0 65 236 78 846.0 0 78 237 60 846.0 0 60 238 123 846.0 0 123 239 107 846.0 0 107 240 853 846.0 0 853 241 277 846.0 0 277 242 448 846.0 0 448 243 114 846.0 0 114 244 91 846.0 0 91 245 107 846.0 0 107 246 67 846.0 0 67 247 53 846.0 0 53 248 32 846.0 0 32 249 85 846.0 0 85 250 152 846.0 0 152 251 221 846.0 0 221 252 155 846.0 0 155 253 118 846.0 0 118 254 116 846.0 0 116 255 100 846.0 0 100 256 70 846.0 0 70 257 50 846.0 0 50 258 65 846.0 0 65 259 70 846.0 0 70 260 124 846.0 0 124 261 482 846.0 0 482 262 317 846.0 0 317 263 265 846.0 0 265 264 271 846.0 0 271 265 174 846.0 0 174 266 516 846.0 0 516 267 97 846.0 0 97 268 141 846.0 0 141 269 131 846.0 0 131 270 198 846.0 0 198 271 171 846.0 0 171 272 117 846.0 0 117 273 60 846.0 0 60 274 136 846.0 0 136 275 67 846.0 0 67 276 174 846.0 0 174 277 180 846.0 0 180 278 150 846.0 0 150 279 72 846.0 0 72 280 68 846.0 0 68 281 118 846.0 0 118 282 211 846.0 0 211 283 286 846.0 0 286 284 91 846.0 0 91 285 60 846.0 0 60 286 182 846.0 0 182 287 63 846.0 0 63 288 105 846.0 0 105 289 235 846.0 0 235 290 243 846.0 0 243 291 411 846.0 0 411 292 372 846.0 0 372 293 119 846.0 0 119 294 189 846.0 0 189 295 407 846.0 0 407 296 251 846.0 0 251 297 123 846.0 0 123 298 143 846.0 0 143 299 209 846.0 0 209 300 178 846.0 0 178 301 33 846.0 0 33 Finished in 9.43 s (39 us/read; 1.53 M reads/minute). === Summary === Total reads processed: 240,844 Reads with adapters: 32,779 (13.6%) Reads that were too short: 230 (0.1%) Reads written (passing filters): 32,549 (13.5%) Total basepairs processed: 54,717,690 bp Total written (filtered): 8,942,335 bp (16.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 32779 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 10 0.0 3 0 0 7 3 16 218 0.0 3 0 196 11 11 17 31889 0.0 3 31055 748 73 13 18 98 0.0 3 2 86 7 3 19 26 0.0 3 2 1 16 7 20 23 0.0 3 0 0 0 23 22 18 0.0 3 0 0 0 18 23 1 0.0 3 0 0 0 1 24 1 0.0 3 0 0 0 1 25 2 0.0 3 0 0 1 1 28 25 0.0 3 0 0 0 25 31 1 0.0 3 0 0 0 1 34 13 0.0 3 0 0 0 13 37 3 0.0 3 1 0 0 2 38 3 0.0 3 0 0 0 3 46 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 49 65 0.0 3 0 0 1 64 50 102 0.0 3 0 0 0 102 51 8 0.0 3 0 0 0 8 52 9 0.0 3 0 0 0 9 53 4 0.0 3 0 0 0 4 55 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 69 8 0.0 3 0 0 0 8 70 1 0.0 3 0 0 0 1 71 2 0.0 3 0 0 0 2 72 5 0.0 3 0 0 0 5 73 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 75 2 0.0 3 0 0 0 2 76 1 0.0 3 0 0 0 1 77 3 0.0 3 0 0 0 3 82 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 101 2 0.0 3 0 0 0 2 103 1 0.0 3 0 0 0 1 106 3 0.0 3 0 0 0 3 108 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 118 2 0.0 3 0 0 0 2 120 2 0.0 3 0 0 0 2 122 1 0.0 3 0 0 0 1 132 2 0.0 3 0 0 0 2 134 1 0.0 3 0 0 0 1 136 2 0.0 3 0 0 0 2 137 3 0.0 3 0 0 0 3 157 1 0.0 3 0 0 0 1 161 16 0.0 3 0 0 1 15 162 36 0.0 3 0 0 2 34 163 12 0.0 3 0 0 0 12 164 25 0.0 3 0 0 0 25 165 84 0.0 3 0 0 5 79 166 5 0.0 3 0 0 1 4 168 1 0.0 3 0 0 0 1 169 2 0.0 3 0 0 0 2 177 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 192 2 0.0 3 0 0 0 2 207 1 0.0 3 0 0 0 1 208 2 0.0 3 0 0 0 2 209 3 0.0 3 0 0 0 3 212 1 0.0 3 0 0 0 1 215 5 0.0 3 0 0 0 5 221 1 0.0 3 0 0 0 1 261 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 282 2 0.0 3 0 0 0 2 283 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.14 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 32,549 Reads with adapters: 744 (2.3%) Reads written (passing filters): 32,549 (100.0%) Total basepairs processed: 8,942,335 bp Total written (filtered): 8,940,065 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 744 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 58.5% C: 18.1% G: 15.9% T: 7.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 712 508.6 0 712 4 27 127.1 0 27 5 4 31.8 0 4 6 1 7.9 0 1