This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCTTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 88.41 s (26 us/read; 2.35 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 152,325 (4.4%) Reads that were too short: 66,536 (1.9%) Reads written (passing filters): 85,789 (2.5%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 22,594,491 bp (2.2%) === Adapter 1 === Sequence: GTCTTA; Type: regular 5'; Length: 6; Trimmed: 152325 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 333 846.0 0 333 7 630 846.0 0 630 8 27042 846.0 0 27042 9 31787 846.0 0 31787 10 71 846.0 0 71 11 14 846.0 0 14 12 9 846.0 0 9 13 6 846.0 0 6 14 7 846.0 0 7 15 10 846.0 0 10 16 7 846.0 0 7 17 8 846.0 0 8 18 19 846.0 0 19 19 59 846.0 0 59 20 21 846.0 0 21 21 519 846.0 0 519 22 69 846.0 0 69 23 26 846.0 0 26 24 8 846.0 0 8 25 10 846.0 0 10 26 9 846.0 0 9 27 21 846.0 0 21 28 20 846.0 0 20 29 15 846.0 0 15 30 21 846.0 0 21 31 5 846.0 0 5 32 23 846.0 0 23 33 39 846.0 0 39 34 12 846.0 0 12 35 11 846.0 0 11 36 106 846.0 0 106 37 6 846.0 0 6 38 19 846.0 0 19 39 9 846.0 0 9 40 4 846.0 0 4 41 7 846.0 0 7 42 7 846.0 0 7 43 5 846.0 0 5 44 13 846.0 0 13 45 10 846.0 0 10 46 9 846.0 0 9 47 6 846.0 0 6 48 13 846.0 0 13 49 24 846.0 0 24 50 9 846.0 0 9 51 8 846.0 0 8 52 11 846.0 0 11 53 10 846.0 0 10 54 6 846.0 0 6 55 17 846.0 0 17 56 34 846.0 0 34 57 100 846.0 0 100 58 4887 846.0 0 4887 59 161 846.0 0 161 60 10 846.0 0 10 61 24 846.0 0 24 62 17 846.0 0 17 63 40 846.0 0 40 64 43 846.0 0 43 65 36 846.0 0 36 66 13 846.0 0 13 67 12 846.0 0 12 68 22 846.0 0 22 69 63 846.0 0 63 70 68 846.0 0 68 71 40 846.0 0 40 72 15 846.0 0 15 73 28 846.0 0 28 74 38 846.0 0 38 75 73 846.0 0 73 76 80 846.0 0 80 77 26 846.0 0 26 78 30 846.0 0 30 79 36 846.0 0 36 80 71 846.0 0 71 81 44 846.0 0 44 82 46 846.0 0 46 83 49 846.0 0 49 84 51 846.0 0 51 85 66 846.0 0 66 86 65 846.0 0 65 87 92 846.0 0 92 88 219 846.0 0 219 89 6094 846.0 0 6094 90 1204 846.0 0 1204 91 913 846.0 0 913 92 12 846.0 0 12 93 19 846.0 0 19 94 39 846.0 0 39 95 32 846.0 0 32 96 65 846.0 0 65 97 73 846.0 0 73 98 164 846.0 0 164 99 55 846.0 0 55 100 62 846.0 0 62 101 94 846.0 0 94 102 97 846.0 0 97 103 61 846.0 0 61 104 25 846.0 0 25 105 24 846.0 0 24 106 9 846.0 0 9 107 13 846.0 0 13 108 14 846.0 0 14 109 19 846.0 0 19 110 20 846.0 0 20 111 49 846.0 0 49 112 54 846.0 0 54 113 84 846.0 0 84 114 218 846.0 0 218 115 46 846.0 0 46 116 45 846.0 0 45 117 15 846.0 0 15 118 16 846.0 0 16 119 11 846.0 0 11 120 24 846.0 0 24 121 39 846.0 0 39 122 52 846.0 0 52 123 22 846.0 0 22 124 42 846.0 0 42 125 101 846.0 0 101 126 60 846.0 0 60 127 37 846.0 0 37 128 37 846.0 0 37 129 34 846.0 0 34 130 30 846.0 0 30 131 35 846.0 0 35 132 52 846.0 0 52 133 168 846.0 0 168 134 55 846.0 0 55 135 45 846.0 0 45 136 22 846.0 0 22 137 30 846.0 0 30 138 33 846.0 0 33 139 86 846.0 0 86 140 49 846.0 0 49 141 58 846.0 0 58 142 38 846.0 0 38 143 26 846.0 0 26 144 50 846.0 0 50 145 84 846.0 0 84 146 83 846.0 0 83 147 75 846.0 0 75 148 384 846.0 0 384 149 260 846.0 0 260 150 294 846.0 0 294 151 28 846.0 0 28 152 34 846.0 0 34 153 50 846.0 0 50 154 36 846.0 0 36 155 27 846.0 0 27 156 38 846.0 0 38 157 64 846.0 0 64 158 52 846.0 0 52 159 64 846.0 0 64 160 46 846.0 0 46 161 53 846.0 0 53 162 55 846.0 0 55 163 27 846.0 0 27 164 45 846.0 0 45 165 43 846.0 0 43 166 38 846.0 0 38 167 43 846.0 0 43 168 557 846.0 0 557 169 217 846.0 0 217 170 421 846.0 0 421 171 23 846.0 0 23 172 35 846.0 0 35 173 40 846.0 0 40 174 63 846.0 0 63 175 59 846.0 0 59 176 1133 846.0 0 1133 177 1252 846.0 0 1252 178 506 846.0 0 506 179 44 846.0 0 44 180 43 846.0 0 43 181 23 846.0 0 23 182 56 846.0 0 56 183 40 846.0 0 40 184 44 846.0 0 44 185 53 846.0 0 53 186 57 846.0 0 57 187 41 846.0 0 41 188 22 846.0 0 22 189 35 846.0 0 35 190 33 846.0 0 33 191 44 846.0 0 44 192 34 846.0 0 34 193 63 846.0 0 63 194 69 846.0 0 69 195 48 846.0 0 48 196 42 846.0 0 42 197 31 846.0 0 31 198 35 846.0 0 35 199 30 846.0 0 30 200 15 846.0 0 15 201 58 846.0 0 58 202 57 846.0 0 57 203 71 846.0 0 71 204 66 846.0 0 66 205 78 846.0 0 78 206 68 846.0 0 68 207 273 846.0 0 273 208 484 846.0 0 484 209 200 846.0 0 200 210 83 846.0 0 83 211 60 846.0 0 60 212 53 846.0 0 53 213 62 846.0 0 62 214 188 846.0 0 188 215 120 846.0 0 120 216 127 846.0 0 127 217 102 846.0 0 102 218 144 846.0 0 144 219 308 846.0 0 308 220 4086 846.0 0 4086 221 3635 846.0 0 3635 222 2317 846.0 0 2317 223 59 846.0 0 59 224 59 846.0 0 59 225 71 846.0 0 71 226 76 846.0 0 76 227 60 846.0 0 60 228 68 846.0 0 68 229 49 846.0 0 49 230 96 846.0 0 96 231 106 846.0 0 106 232 84 846.0 0 84 233 57 846.0 0 57 234 79 846.0 0 79 235 55 846.0 0 55 236 52 846.0 0 52 237 84 846.0 0 84 238 127 846.0 0 127 239 241 846.0 0 241 240 214 846.0 0 214 241 302 846.0 0 302 242 134 846.0 0 134 243 92 846.0 0 92 244 124 846.0 0 124 245 88 846.0 0 88 246 191 846.0 0 191 247 75 846.0 0 75 248 64 846.0 0 64 249 40 846.0 0 40 250 36 846.0 0 36 251 44 846.0 0 44 252 35 846.0 0 35 253 76 846.0 0 76 254 99 846.0 0 99 255 163 846.0 0 163 256 277 846.0 0 277 257 367 846.0 0 367 258 112 846.0 0 112 259 73 846.0 0 73 260 78 846.0 0 78 261 78 846.0 0 78 262 59 846.0 0 59 263 79 846.0 0 79 264 88 846.0 0 88 265 113 846.0 0 113 266 188 846.0 0 188 267 85 846.0 0 85 268 127 846.0 0 127 269 449 846.0 0 449 270 316 846.0 0 316 271 268 846.0 0 268 272 135 846.0 0 135 273 136 846.0 0 136 274 519 846.0 0 519 275 685 846.0 0 685 276 1206 846.0 0 1206 277 91 846.0 0 91 278 107 846.0 0 107 279 196 846.0 0 196 280 310 846.0 0 310 281 751 846.0 0 751 282 928 846.0 0 928 283 19654 846.0 0 19654 284 11903 846.0 0 11903 285 9142 846.0 0 9142 286 531 846.0 0 531 287 81 846.0 0 81 288 419 846.0 0 419 289 69 846.0 0 69 290 92 846.0 0 92 291 58 846.0 0 58 292 100 846.0 0 100 293 170 846.0 0 170 294 167 846.0 0 167 295 83 846.0 0 83 296 255 846.0 0 255 297 27 846.0 0 27 298 30 846.0 0 30 299 30 846.0 0 30 300 16 846.0 0 16 301 106 846.0 0 106 Finished in 4.19 s (49 us/read; 1.23 M reads/minute). === Summary === Total reads processed: 85,789 Reads with adapters: 32,561 (38.0%) Reads that were too short: 14 (0.0%) Reads written (passing filters): 32,547 (37.9%) Total basepairs processed: 22,594,491 bp Total written (filtered): 8,843,931 bp (39.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 32561 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 0 2 18 5 0.0 3 0 0 5 19 108 0.0 3 0 103 2 2 1 20 32223 0.0 4 31870 345 5 2 1 21 81 0.0 4 13 66 2 22 5 0.0 4 1 0 4 23 16 0.0 4 1 0 0 14 1 24 11 0.0 4 0 0 0 0 11 25 1 0.0 4 1 26 1 0.0 4 1 37 1 0.0 4 1 54 1 0.0 4 0 0 0 0 1 56 1 0.0 4 0 0 0 0 1 67 2 0.0 4 0 0 0 0 2 86 1 0.0 4 1 91 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 122 1 0.0 4 0 0 0 0 1 127 88 0.0 4 0 0 0 0 88 128 1 0.0 4 0 0 0 0 1 139 1 0.0 4 0 0 0 0 1 146 1 0.0 4 0 0 0 0 1 195 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 210 2 0.0 4 0 0 0 0 2 227 1 0.0 4 0 0 0 0 1 237 1 0.0 4 0 0 0 0 1 242 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.10 s (34 us/read; 1.78 M reads/minute). === Summary === Total reads processed: 32,547 Reads with adapters: 4,397 (13.5%) Reads written (passing filters): 32,547 (100.0%) Total basepairs processed: 8,843,931 bp Total written (filtered): 7,886,975 bp (89.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 4397 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.4% C: 80.5% G: 18.3% T: 0.8% none/other: 0.0% WARNING: The adapter is preceded by "C" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "C" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 11 508.5 0 11 4 5 127.1 0 5 5 2 31.8 0 2 6 2 7.9 0 2 9 102 0.1 0 7 95 10 473 0.0 1 17 456 11 151 0.0 1 2 149 150 3 0.0 1 0 3 199 1 0.0 1 1 228 3 0.0 1 3 244 1 0.0 1 0 1 255 2 0.0 1 2 256 581 0.0 1 559 22 257 10 0.0 1 10 258 4 0.0 1 4 260 11 0.0 1 11 261 2959 0.0 1 2839 120 262 37 0.0 1 33 4 263 14 0.0 1 14 264 23 0.0 1 23 269 1 0.0 1 1 271 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.