This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACCATA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 90.69 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 300,225 (8.7%) Reads that were too short: 135,144 (3.9%) Reads written (passing filters): 165,081 (4.8%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 27,125,877 bp (2.6%) === Adapter 1 === Sequence: ACCATA; Type: regular 5'; Length: 6; Trimmed: 300225 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 107 846.0 0 107 7 6802 846.0 0 6802 8 82 846.0 0 82 9 60 846.0 0 60 10 94 846.0 0 94 11 71 846.0 0 71 12 68 846.0 0 68 13 72 846.0 0 72 14 66 846.0 0 66 15 58 846.0 0 58 16 66 846.0 0 66 17 64 846.0 0 64 18 64 846.0 0 64 19 76 846.0 0 76 20 75 846.0 0 75 21 90 846.0 0 90 22 63 846.0 0 63 23 80 846.0 0 80 24 74 846.0 0 74 25 79 846.0 0 79 26 62 846.0 0 62 27 73 846.0 0 73 28 68 846.0 0 68 29 69 846.0 0 69 30 50 846.0 0 50 31 84 846.0 0 84 32 51 846.0 0 51 33 74 846.0 0 74 34 92 846.0 0 92 35 66 846.0 0 66 36 80 846.0 0 80 37 64 846.0 0 64 38 59 846.0 0 59 39 92 846.0 0 92 40 53 846.0 0 53 41 83 846.0 0 83 42 638 846.0 0 638 43 82 846.0 0 82 44 64 846.0 0 64 45 77 846.0 0 77 46 67 846.0 0 67 47 72 846.0 0 72 48 69 846.0 0 69 49 65 846.0 0 65 50 65 846.0 0 65 51 67 846.0 0 67 52 60 846.0 0 60 53 68 846.0 0 68 54 57 846.0 0 57 55 62 846.0 0 62 56 74 846.0 0 74 57 79 846.0 0 79 58 71 846.0 0 71 59 49 846.0 0 49 60 90 846.0 0 90 61 72 846.0 0 72 62 62 846.0 0 62 63 81 846.0 0 81 64 64 846.0 0 64 65 63 846.0 0 63 66 92 846.0 0 92 67 88 846.0 0 88 68 84 846.0 0 84 69 157 846.0 0 157 70 74 846.0 0 74 71 66 846.0 0 66 72 63 846.0 0 63 73 103 846.0 0 103 74 265 846.0 0 265 75 55 846.0 0 55 76 150 846.0 0 150 77 78 846.0 0 78 78 129 846.0 0 129 79 129 846.0 0 129 80 187 846.0 0 187 81 223 846.0 0 223 82 452 846.0 0 452 83 569 846.0 0 569 84 639 846.0 0 639 85 475 846.0 0 475 86 469 846.0 0 469 87 1724 846.0 0 1724 88 1033 846.0 0 1033 89 785 846.0 0 785 90 756 846.0 0 756 91 444 846.0 0 444 92 341 846.0 0 341 93 1674 846.0 0 1674 94 2092 846.0 0 2092 95 2061 846.0 0 2061 96 658 846.0 0 658 97 393 846.0 0 393 98 523 846.0 0 523 99 315 846.0 0 315 100 337 846.0 0 337 101 501 846.0 0 501 102 455 846.0 0 455 103 599 846.0 0 599 104 698 846.0 0 698 105 497 846.0 0 497 106 670 846.0 0 670 107 1550 846.0 0 1550 108 2052 846.0 0 2052 109 2668 846.0 0 2668 110 489 846.0 0 489 111 623 846.0 0 623 112 516 846.0 0 516 113 614 846.0 0 614 114 699 846.0 0 699 115 593 846.0 0 593 116 450 846.0 0 450 117 369 846.0 0 369 118 397 846.0 0 397 119 646 846.0 0 646 120 1243 846.0 0 1243 121 1051 846.0 0 1051 122 574 846.0 0 574 123 585 846.0 0 585 124 619 846.0 0 619 125 748 846.0 0 748 126 1012 846.0 0 1012 127 1102 846.0 0 1102 128 963 846.0 0 963 129 1280 846.0 0 1280 130 550 846.0 0 550 131 483 846.0 0 483 132 658 846.0 0 658 133 2030 846.0 0 2030 134 2970 846.0 0 2970 135 3521 846.0 0 3521 136 4998 846.0 0 4998 137 880 846.0 0 880 138 653 846.0 0 653 139 896 846.0 0 896 140 1126 846.0 0 1126 141 1409 846.0 0 1409 142 852 846.0 0 852 143 548 846.0 0 548 144 769 846.0 0 769 145 1275 846.0 0 1275 146 1351 846.0 0 1351 147 1283 846.0 0 1283 148 1373 846.0 0 1373 149 885 846.0 0 885 150 854 846.0 0 854 151 953 846.0 0 953 152 1558 846.0 0 1558 153 1762 846.0 0 1762 154 1549 846.0 0 1549 155 1936 846.0 0 1936 156 1965 846.0 0 1965 157 9315 846.0 0 9315 158 14616 846.0 0 14616 159 4657 846.0 0 4657 160 1418 846.0 0 1418 161 1296 846.0 0 1296 162 927 846.0 0 927 163 683 846.0 0 683 164 1024 846.0 0 1024 165 1634 846.0 0 1634 166 1500 846.0 0 1500 167 1005 846.0 0 1005 168 654 846.0 0 654 169 723 846.0 0 723 170 703 846.0 0 703 171 854 846.0 0 854 172 905 846.0 0 905 173 2013 846.0 0 2013 174 2590 846.0 0 2590 175 1415 846.0 0 1415 176 760 846.0 0 760 177 556 846.0 0 556 178 544 846.0 0 544 179 637 846.0 0 637 180 1147 846.0 0 1147 181 1521 846.0 0 1521 182 1227 846.0 0 1227 183 758 846.0 0 758 184 712 846.0 0 712 185 970 846.0 0 970 186 1869 846.0 0 1869 187 1160 846.0 0 1160 188 771 846.0 0 771 189 646 846.0 0 646 190 1060 846.0 0 1060 191 1509 846.0 0 1509 192 1344 846.0 0 1344 193 1224 846.0 0 1224 194 724 846.0 0 724 195 644 846.0 0 644 196 464 846.0 0 464 197 505 846.0 0 505 198 901 846.0 0 901 199 761 846.0 0 761 200 927 846.0 0 927 201 1226 846.0 0 1226 202 897 846.0 0 897 203 1580 846.0 0 1580 204 1024 846.0 0 1024 205 755 846.0 0 755 206 939 846.0 0 939 207 1014 846.0 0 1014 208 998 846.0 0 998 209 659 846.0 0 659 210 635 846.0 0 635 211 533 846.0 0 533 212 662 846.0 0 662 213 1008 846.0 0 1008 214 1153 846.0 0 1153 215 937 846.0 0 937 216 1111 846.0 0 1111 217 1044 846.0 0 1044 218 959 846.0 0 959 219 1032 846.0 0 1032 220 1307 846.0 0 1307 221 1450 846.0 0 1450 222 813 846.0 0 813 223 554 846.0 0 554 224 752 846.0 0 752 225 824 846.0 0 824 226 901 846.0 0 901 227 997 846.0 0 997 228 1044 846.0 0 1044 229 959 846.0 0 959 230 982 846.0 0 982 231 983 846.0 0 983 232 1051 846.0 0 1051 233 1230 846.0 0 1230 234 1022 846.0 0 1022 235 852 846.0 0 852 236 1132 846.0 0 1132 237 840 846.0 0 840 238 708 846.0 0 708 239 814 846.0 0 814 240 861 846.0 0 861 241 962 846.0 0 962 242 932 846.0 0 932 243 971 846.0 0 971 244 897 846.0 0 897 245 796 846.0 0 796 246 922 846.0 0 922 247 850 846.0 0 850 248 858 846.0 0 858 249 443 846.0 0 443 250 693 846.0 0 693 251 853 846.0 0 853 252 2029 846.0 0 2029 253 2823 846.0 0 2823 254 1272 846.0 0 1272 255 783 846.0 0 783 256 1049 846.0 0 1049 257 1211 846.0 0 1211 258 1030 846.0 0 1030 259 791 846.0 0 791 260 1034 846.0 0 1034 261 966 846.0 0 966 262 962 846.0 0 962 263 815 846.0 0 815 264 690 846.0 0 690 265 620 846.0 0 620 266 1374 846.0 0 1374 267 1329 846.0 0 1329 268 1687 846.0 0 1687 269 1566 846.0 0 1566 270 1001 846.0 0 1001 271 1026 846.0 0 1026 272 1156 846.0 0 1156 273 1010 846.0 0 1010 274 884 846.0 0 884 275 1098 846.0 0 1098 276 2752 846.0 0 2752 277 4886 846.0 0 4886 278 2276 846.0 0 2276 279 1839 846.0 0 1839 280 1651 846.0 0 1651 281 1917 846.0 0 1917 282 982 846.0 0 982 283 716 846.0 0 716 284 1291 846.0 0 1291 285 1209 846.0 0 1209 286 1129 846.0 0 1129 287 1141 846.0 0 1141 288 1306 846.0 0 1306 289 1044 846.0 0 1044 290 874 846.0 0 874 291 905 846.0 0 905 292 1213 846.0 0 1213 293 1505 846.0 0 1505 294 1974 846.0 0 1974 295 4312 846.0 0 4312 296 7313 846.0 0 7313 297 14596 846.0 0 14596 298 594 846.0 0 594 299 542 846.0 0 542 300 800 846.0 0 800 301 1978 846.0 0 1978 Finished in 5.81 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 165,081 Reads with adapters: 4,309 (2.6%) Reads that were too short: 40 (0.0%) Reads written (passing filters): 4,269 (2.6%) Total basepairs processed: 27,125,877 bp Total written (filtered): 1,167,547 bp (4.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 4309 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 1 0.0 3 0 0 1 19 19 0.0 3 0 18 0 0 1 20 4226 0.0 4 3842 370 13 1 21 4 0.0 4 1 3 22 2 0.0 4 0 0 0 0 2 23 7 0.0 4 0 0 0 4 3 31 1 0.0 4 0 0 0 0 1 32 1 0.0 4 0 0 0 0 1 35 1 0.0 4 0 0 0 0 1 37 1 0.0 4 0 0 0 0 1 40 1 0.0 4 0 0 0 0 1 44 1 0.0 4 0 0 0 0 1 46 1 0.0 4 0 0 0 0 1 52 1 0.0 4 0 0 0 0 1 55 1 0.0 4 0 0 0 0 1 58 1 0.0 4 0 0 0 0 1 60 1 0.0 4 0 0 0 0 1 61 1 0.0 4 0 0 0 0 1 65 1 0.0 4 0 0 0 0 1 66 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 0 1 76 1 0.0 4 0 0 0 0 1 77 1 0.0 4 0 0 0 0 1 80 1 0.0 4 0 0 0 0 1 81 3 0.0 4 0 0 0 0 3 82 1 0.0 4 0 0 0 0 1 84 3 0.0 4 0 0 0 0 3 86 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 92 3 0.0 4 0 0 0 0 3 93 1 0.0 4 0 0 0 0 1 100 1 0.0 4 0 0 0 0 1 101 2 0.0 4 0 0 0 0 2 102 2 0.0 4 0 0 0 0 2 103 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 0 1 115 1 0.0 4 0 0 0 0 1 129 1 0.0 4 0 0 0 0 1 133 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 0 1 158 1 0.0 4 0 0 0 0 1 163 1 0.0 4 0 0 0 0 1 178 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 1 211 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.14 s (33 us/read; 1.83 M reads/minute). === Summary === Total reads processed: 4,269 Reads with adapters: 95 (2.2%) Reads written (passing filters): 4,269 (100.0%) Total basepairs processed: 1,167,547 bp Total written (filtered): 1,160,861 bp (99.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 95 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 20.0% C: 1.1% G: 77.9% T: 1.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 5 1 4.2 0 1 11 69 0.0 1 0 69 12 2 0.0 1 1 1 152 1 0.0 1 0 1 261 18 0.0 1 17 1 262 4 0.0 1 4