This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCCGCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 87.63 s (25 us/read; 2.37 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 55,897 (1.6%) Reads that were too short: 15,926 (0.5%) Reads written (passing filters): 39,971 (1.2%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 8,699,572 bp (0.8%) === Adapter 1 === Sequence: TCCGCT; Type: regular 5'; Length: 6; Trimmed: 55897 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 208 846.0 0 208 7 367 846.0 0 367 8 15024 846.0 0 15024 9 72 846.0 0 72 10 59 846.0 0 59 11 15 846.0 0 15 12 15 846.0 0 15 13 15 846.0 0 15 14 13 846.0 0 13 15 13 846.0 0 13 16 59 846.0 0 59 17 58 846.0 0 58 18 63 846.0 0 63 19 17 846.0 0 17 20 11 846.0 0 11 21 14 846.0 0 14 22 11 846.0 0 11 23 26 846.0 0 26 24 11 846.0 0 11 25 34 846.0 0 34 26 15 846.0 0 15 27 12 846.0 0 12 28 16 846.0 0 16 29 15 846.0 0 15 30 16 846.0 0 16 31 16 846.0 0 16 32 46 846.0 0 46 33 26 846.0 0 26 34 100 846.0 0 100 35 23 846.0 0 23 36 19 846.0 0 19 37 129 846.0 0 129 38 140 846.0 0 140 39 46 846.0 0 46 40 43 846.0 0 43 41 127 846.0 0 127 42 160 846.0 0 160 43 25 846.0 0 25 44 15 846.0 0 15 45 16 846.0 0 16 46 18 846.0 0 18 47 14 846.0 0 14 48 24 846.0 0 24 49 42 846.0 0 42 50 19 846.0 0 19 51 24 846.0 0 24 52 346 846.0 0 346 53 186 846.0 0 186 54 51 846.0 0 51 55 17 846.0 0 17 56 16 846.0 0 16 57 23 846.0 0 23 58 8 846.0 0 8 59 19 846.0 0 19 60 26 846.0 0 26 61 88 846.0 0 88 62 29 846.0 0 29 63 9 846.0 0 9 64 10 846.0 0 10 65 24 846.0 0 24 66 25 846.0 0 25 67 23 846.0 0 23 68 15 846.0 0 15 69 12 846.0 0 12 70 13 846.0 0 13 71 76 846.0 0 76 72 119 846.0 0 119 73 203 846.0 0 203 74 7 846.0 0 7 75 10 846.0 0 10 76 13 846.0 0 13 77 15 846.0 0 15 78 12 846.0 0 12 79 16 846.0 0 16 80 16 846.0 0 16 81 26 846.0 0 26 82 25 846.0 0 25 83 24 846.0 0 24 84 281 846.0 0 281 85 77 846.0 0 77 86 89 846.0 0 89 87 23 846.0 0 23 88 17 846.0 0 17 89 8 846.0 0 8 90 39 846.0 0 39 91 40 846.0 0 40 92 42 846.0 0 42 93 179 846.0 0 179 94 190 846.0 0 190 95 59 846.0 0 59 96 39 846.0 0 39 97 29 846.0 0 29 98 21 846.0 0 21 99 28 846.0 0 28 100 11 846.0 0 11 101 13 846.0 0 13 102 31 846.0 0 31 103 18 846.0 0 18 104 43 846.0 0 43 105 37 846.0 0 37 106 27 846.0 0 27 107 28 846.0 0 28 108 196 846.0 0 196 109 36 846.0 0 36 110 120 846.0 0 120 111 515 846.0 0 515 112 42 846.0 0 42 113 39 846.0 0 39 114 43 846.0 0 43 115 32 846.0 0 32 116 26 846.0 0 26 117 48 846.0 0 48 118 64 846.0 0 64 119 78 846.0 0 78 120 24 846.0 0 24 121 12 846.0 0 12 122 31 846.0 0 31 123 34 846.0 0 34 124 37 846.0 0 37 125 67 846.0 0 67 126 47 846.0 0 47 127 203 846.0 0 203 128 162 846.0 0 162 129 79 846.0 0 79 130 27 846.0 0 27 131 34 846.0 0 34 132 64 846.0 0 64 133 63 846.0 0 63 134 50 846.0 0 50 135 33 846.0 0 33 136 33 846.0 0 33 137 33 846.0 0 33 138 61 846.0 0 61 139 62 846.0 0 62 140 84 846.0 0 84 141 147 846.0 0 147 142 120 846.0 0 120 143 248 846.0 0 248 144 6575 846.0 0 6575 145 2892 846.0 0 2892 146 3091 846.0 0 3091 147 167 846.0 0 167 148 17 846.0 0 17 149 41 846.0 0 41 150 62 846.0 0 62 151 172 846.0 0 172 152 115 846.0 0 115 153 120 846.0 0 120 154 113 846.0 0 113 155 48 846.0 0 48 156 61 846.0 0 61 157 189 846.0 0 189 158 84 846.0 0 84 159 160 846.0 0 160 160 32 846.0 0 32 161 35 846.0 0 35 162 31 846.0 0 31 163 16 846.0 0 16 164 34 846.0 0 34 165 67 846.0 0 67 166 44 846.0 0 44 167 24 846.0 0 24 168 23 846.0 0 23 169 42 846.0 0 42 170 63 846.0 0 63 171 122 846.0 0 122 172 98 846.0 0 98 173 77 846.0 0 77 174 52 846.0 0 52 175 46 846.0 0 46 176 70 846.0 0 70 177 62 846.0 0 62 178 59 846.0 0 59 179 288 846.0 0 288 180 297 846.0 0 297 181 151 846.0 0 151 182 32 846.0 0 32 183 24 846.0 0 24 184 29 846.0 0 29 185 41 846.0 0 41 186 38 846.0 0 38 187 28 846.0 0 28 188 21 846.0 0 21 189 27 846.0 0 27 190 69 846.0 0 69 191 236 846.0 0 236 192 124 846.0 0 124 193 42 846.0 0 42 194 36 846.0 0 36 195 22 846.0 0 22 196 105 846.0 0 105 197 105 846.0 0 105 198 124 846.0 0 124 199 62 846.0 0 62 200 24 846.0 0 24 201 26 846.0 0 26 202 26 846.0 0 26 203 23 846.0 0 23 204 29 846.0 0 29 205 41 846.0 0 41 206 43 846.0 0 43 207 30 846.0 0 30 208 19 846.0 0 19 209 15 846.0 0 15 210 26 846.0 0 26 211 24 846.0 0 24 212 30 846.0 0 30 213 38 846.0 0 38 214 26 846.0 0 26 215 36 846.0 0 36 216 25 846.0 0 25 217 41 846.0 0 41 218 32 846.0 0 32 219 43 846.0 0 43 220 21 846.0 0 21 221 35 846.0 0 35 222 24 846.0 0 24 223 31 846.0 0 31 224 36 846.0 0 36 225 51 846.0 0 51 226 585 846.0 0 585 227 42 846.0 0 42 228 76 846.0 0 76 229 50 846.0 0 50 230 60 846.0 0 60 231 239 846.0 0 239 232 44 846.0 0 44 233 32 846.0 0 32 234 35 846.0 0 35 235 148 846.0 0 148 236 195 846.0 0 195 237 234 846.0 0 234 238 120 846.0 0 120 239 48 846.0 0 48 240 284 846.0 0 284 241 2431 846.0 0 2431 242 140 846.0 0 140 243 21 846.0 0 21 244 30 846.0 0 30 245 60 846.0 0 60 246 48 846.0 0 48 247 73 846.0 0 73 248 57 846.0 0 57 249 79 846.0 0 79 250 506 846.0 0 506 251 85 846.0 0 85 252 150 846.0 0 150 253 135 846.0 0 135 254 74 846.0 0 74 255 79 846.0 0 79 256 75 846.0 0 75 257 47 846.0 0 47 258 67 846.0 0 67 259 27 846.0 0 27 260 305 846.0 0 305 261 2290 846.0 0 2290 262 3457 846.0 0 3457 263 297 846.0 0 297 264 39 846.0 0 39 265 53 846.0 0 53 266 59 846.0 0 59 267 59 846.0 0 59 268 71 846.0 0 71 269 26 846.0 0 26 270 65 846.0 0 65 271 51 846.0 0 51 272 51 846.0 0 51 273 25 846.0 0 25 274 18 846.0 0 18 275 8 846.0 0 8 276 24 846.0 0 24 277 65 846.0 0 65 278 35 846.0 0 35 279 38 846.0 0 38 280 42 846.0 0 42 281 70 846.0 0 70 282 34 846.0 0 34 283 24 846.0 0 24 284 199 846.0 0 199 285 106 846.0 0 106 286 39 846.0 0 39 287 23 846.0 0 23 288 53 846.0 0 53 289 47 846.0 0 47 290 26 846.0 0 26 291 110 846.0 0 110 292 421 846.0 0 421 293 231 846.0 0 231 294 107 846.0 0 107 295 90 846.0 0 90 296 77 846.0 0 77 297 18 846.0 0 18 298 14 846.0 0 14 299 13 846.0 0 13 300 8 846.0 0 8 301 17 846.0 0 17 Finished in 1.60 s (40 us/read; 1.50 M reads/minute). === Summary === Total reads processed: 39,971 Reads with adapters: 14,027 (35.1%) Reads that were too short: 17 (0.0%) Reads written (passing filters): 14,010 (35.1%) Total basepairs processed: 8,699,572 bp Total written (filtered): 3,863,592 bp (44.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 14027 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 7 0.0 3 0 0 4 3 16 110 0.0 3 0 95 9 6 17 13804 0.0 3 13416 347 34 7 18 35 0.0 3 0 33 1 1 19 17 0.0 3 1 1 8 7 20 9 0.0 3 0 0 0 9 25 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 32 1 0.0 3 1 33 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 55 2 0.0 3 0 0 0 2 73 1 0.0 3 0 0 0 1 81 2 0.0 3 0 0 0 2 85 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 109 2 0.0 3 0 0 0 2 111 2 0.0 3 0 0 0 2 118 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 120 3 0.0 3 0 0 0 3 121 1 0.0 3 0 0 0 1 124 2 0.0 3 0 0 1 1 128 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 175 2 0.0 3 0 0 0 2 178 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 228 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.49 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 14,010 Reads with adapters: 204 (1.5%) Reads written (passing filters): 14,010 (100.0%) Total basepairs processed: 3,863,592 bp Total written (filtered): 3,862,975 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 204 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 34.8% C: 44.1% G: 9.8% T: 11.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 199 218.9 0 199 4 5 54.7 0 5