This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCAAGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 88.39 s (26 us/read; 2.35 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 64,586 (1.9%) Reads that were too short: 10,212 (0.3%) Reads written (passing filters): 54,374 (1.6%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 12,032,893 bp (1.2%) === Adapter 1 === Sequence: CCAAGG; Type: regular 5'; Length: 6; Trimmed: 64586 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 843 846.0 0 843 7 277 846.0 0 277 8 14778 846.0 0 14778 9 13684 846.0 0 13684 10 23 846.0 0 23 11 6 846.0 0 6 12 1 846.0 0 1 13 7 846.0 0 7 14 3 846.0 0 3 15 4 846.0 0 4 16 5 846.0 0 5 17 5 846.0 0 5 18 15 846.0 0 15 19 11 846.0 0 11 20 18 846.0 0 18 21 11 846.0 0 11 22 1 846.0 0 1 23 8 846.0 0 8 24 1 846.0 0 1 25 6 846.0 0 6 26 2 846.0 0 2 27 7 846.0 0 7 28 5 846.0 0 5 29 3 846.0 0 3 30 6 846.0 0 6 31 5 846.0 0 5 32 6 846.0 0 6 33 7 846.0 0 7 34 2 846.0 0 2 35 8 846.0 0 8 36 3 846.0 0 3 37 4 846.0 0 4 38 4 846.0 0 4 39 5 846.0 0 5 40 5 846.0 0 5 41 5 846.0 0 5 42 5 846.0 0 5 43 10 846.0 0 10 44 10 846.0 0 10 45 44 846.0 0 44 46 11 846.0 0 11 47 1 846.0 0 1 48 8 846.0 0 8 49 7 846.0 0 7 50 12 846.0 0 12 51 9 846.0 0 9 52 2 846.0 0 2 53 4 846.0 0 4 54 10 846.0 0 10 55 3 846.0 0 3 56 6 846.0 0 6 57 3 846.0 0 3 58 9 846.0 0 9 59 4 846.0 0 4 60 16 846.0 0 16 61 2 846.0 0 2 62 7 846.0 0 7 63 5 846.0 0 5 64 7 846.0 0 7 65 8 846.0 0 8 66 22 846.0 0 22 67 5 846.0 0 5 68 5 846.0 0 5 69 4 846.0 0 4 70 4 846.0 0 4 71 4 846.0 0 4 72 10 846.0 0 10 73 17 846.0 0 17 74 26 846.0 0 26 75 36 846.0 0 36 76 80 846.0 0 80 77 2100 846.0 0 2100 78 421 846.0 0 421 79 624 846.0 0 624 80 4 846.0 0 4 81 3 846.0 0 3 82 5 846.0 0 5 83 10 846.0 0 10 84 10 846.0 0 10 85 6 846.0 0 6 86 10 846.0 0 10 87 4 846.0 0 4 88 13 846.0 0 13 89 12 846.0 0 12 90 29 846.0 0 29 91 37 846.0 0 37 92 48 846.0 0 48 93 48 846.0 0 48 94 26 846.0 0 26 95 45 846.0 0 45 96 35 846.0 0 35 97 39 846.0 0 39 98 48 846.0 0 48 99 16 846.0 0 16 100 12 846.0 0 12 101 8 846.0 0 8 102 22 846.0 0 22 103 1 846.0 0 1 105 7 846.0 0 7 106 24 846.0 0 24 107 18 846.0 0 18 108 10 846.0 0 10 109 12 846.0 0 12 110 9 846.0 0 9 111 8 846.0 0 8 112 7 846.0 0 7 113 17 846.0 0 17 114 67 846.0 0 67 115 84 846.0 0 84 116 51 846.0 0 51 117 32 846.0 0 32 118 27 846.0 0 27 119 12 846.0 0 12 120 13 846.0 0 13 121 22 846.0 0 22 122 38 846.0 0 38 123 29 846.0 0 29 124 17 846.0 0 17 125 11 846.0 0 11 126 34 846.0 0 34 127 25 846.0 0 25 128 39 846.0 0 39 129 81 846.0 0 81 130 15 846.0 0 15 131 15 846.0 0 15 132 12 846.0 0 12 133 22 846.0 0 22 134 48 846.0 0 48 135 165 846.0 0 165 136 193 846.0 0 193 137 103 846.0 0 103 138 39 846.0 0 39 139 147 846.0 0 147 140 104 846.0 0 104 141 36 846.0 0 36 142 234 846.0 0 234 143 46 846.0 0 46 144 29 846.0 0 29 145 15 846.0 0 15 146 26 846.0 0 26 147 17 846.0 0 17 148 80 846.0 0 80 149 24 846.0 0 24 150 30 846.0 0 30 151 20 846.0 0 20 152 20 846.0 0 20 153 28 846.0 0 28 154 33 846.0 0 33 155 35 846.0 0 35 156 31 846.0 0 31 157 38 846.0 0 38 158 110 846.0 0 110 159 31 846.0 0 31 160 18 846.0 0 18 161 26 846.0 0 26 162 36 846.0 0 36 163 43 846.0 0 43 164 50 846.0 0 50 165 32 846.0 0 32 166 50 846.0 0 50 167 42 846.0 0 42 168 34 846.0 0 34 169 33 846.0 0 33 170 27 846.0 0 27 171 36 846.0 0 36 172 93 846.0 0 93 173 207 846.0 0 207 174 223 846.0 0 223 175 373 846.0 0 373 176 68 846.0 0 68 177 71 846.0 0 71 178 85 846.0 0 85 179 52 846.0 0 52 180 235 846.0 0 235 181 348 846.0 0 348 182 225 846.0 0 225 183 59 846.0 0 59 184 54 846.0 0 54 185 50 846.0 0 50 186 47 846.0 0 47 187 82 846.0 0 82 188 93 846.0 0 93 189 156 846.0 0 156 190 331 846.0 0 331 191 2927 846.0 0 2927 192 9074 846.0 0 9074 193 2346 846.0 0 2346 194 95 846.0 0 95 195 43 846.0 0 43 196 45 846.0 0 45 197 78 846.0 0 78 198 42 846.0 0 42 199 63 846.0 0 63 200 58 846.0 0 58 201 48 846.0 0 48 202 287 846.0 0 287 203 264 846.0 0 264 204 223 846.0 0 223 205 164 846.0 0 164 206 29 846.0 0 29 207 40 846.0 0 40 208 84 846.0 0 84 209 62 846.0 0 62 210 58 846.0 0 58 211 26 846.0 0 26 212 17 846.0 0 17 213 22 846.0 0 22 214 21 846.0 0 21 215 26 846.0 0 26 216 39 846.0 0 39 217 32 846.0 0 32 218 22 846.0 0 22 219 23 846.0 0 23 220 18 846.0 0 18 221 36 846.0 0 36 222 17 846.0 0 17 223 18 846.0 0 18 224 19 846.0 0 19 225 38 846.0 0 38 226 34 846.0 0 34 227 31 846.0 0 31 228 23 846.0 0 23 229 24 846.0 0 24 230 20 846.0 0 20 231 24 846.0 0 24 232 25 846.0 0 25 233 26 846.0 0 26 234 43 846.0 0 43 235 49 846.0 0 49 236 28 846.0 0 28 237 23 846.0 0 23 238 30 846.0 0 30 239 13 846.0 0 13 240 13 846.0 0 13 241 12 846.0 0 12 242 21 846.0 0 21 243 6 846.0 0 6 244 19 846.0 0 19 245 16 846.0 0 16 246 21 846.0 0 21 247 18 846.0 0 18 248 24 846.0 0 24 249 45 846.0 0 45 250 110 846.0 0 110 251 58 846.0 0 58 252 59 846.0 0 59 253 56 846.0 0 56 254 70 846.0 0 70 255 34 846.0 0 34 256 26 846.0 0 26 257 28 846.0 0 28 258 239 846.0 0 239 259 476 846.0 0 476 260 251 846.0 0 251 261 315 846.0 0 315 262 356 846.0 0 356 263 136 846.0 0 136 264 499 846.0 0 499 265 203 846.0 0 203 266 501 846.0 0 501 267 24 846.0 0 24 268 37 846.0 0 37 269 99 846.0 0 99 270 387 846.0 0 387 271 38 846.0 0 38 272 38 846.0 0 38 273 17 846.0 0 17 274 19 846.0 0 19 275 33 846.0 0 33 276 45 846.0 0 45 277 44 846.0 0 44 278 37 846.0 0 37 279 29 846.0 0 29 280 27 846.0 0 27 281 67 846.0 0 67 282 32 846.0 0 32 283 59 846.0 0 59 284 274 846.0 0 274 285 291 846.0 0 291 286 487 846.0 0 487 287 39 846.0 0 39 288 60 846.0 0 60 289 618 846.0 0 618 290 580 846.0 0 580 291 90 846.0 0 90 292 43 846.0 0 43 293 65 846.0 0 65 294 19 846.0 0 19 295 381 846.0 0 381 296 273 846.0 0 273 297 304 846.0 0 304 298 34 846.0 0 34 299 14 846.0 0 14 300 11 846.0 0 11 301 7 846.0 0 7 Finished in 2.43 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 54,374 Reads with adapters: 14,216 (26.1%) Reads that were too short: 6 (0.0%) Reads written (passing filters): 14,210 (26.1%) Total basepairs processed: 12,032,893 bp Total written (filtered): 3,865,371 bp (32.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 14216 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 31 0.0 3 0 0 0 0 31 18 6 0.0 3 0 0 4 0 2 19 288 0.0 3 0 280 4 2 2 20 13822 0.0 4 13636 182 2 0 2 21 40 0.0 4 17 21 0 0 2 22 4 0.0 4 0 1 2 1 23 7 0.0 4 3 0 0 4 24 1 0.0 4 0 0 0 0 1 26 1 0.0 4 0 0 0 1 29 1 0.0 4 1 33 1 0.0 4 0 0 0 0 1 58 1 0.0 4 0 0 0 1 67 6 0.0 4 0 0 0 0 6 69 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 127 1 0.0 4 0 0 0 0 1 130 1 0.0 4 0 0 0 0 1 228 2 0.0 4 0 0 0 0 2 259 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.48 s (34 us/read; 1.78 M reads/minute). === Summary === Total reads processed: 14,210 Reads with adapters: 383 (2.7%) Reads written (passing filters): 14,210 (100.0%) Total basepairs processed: 3,865,371 bp Total written (filtered): 3,856,820 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 383 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.1% C: 4.2% G: 92.2% T: 1.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 25 222.0 0 25 4 9 55.5 0 9 6 1 3.5 0 1 9 37 0.1 0 2 35 10 257 0.0 1 9 248 11 34 0.0 1 0 34 122 1 0.0 1 0 1 257 1 0.0 1 1 258 1 0.0 1 1 261 7 0.0 1 7 262 1 0.0 1 1 270 8 0.0 1 8 271 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.