This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACAGGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 89.34 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 66,773 (1.9%) Reads that were too short: 19,679 (0.6%) Reads written (passing filters): 47,094 (1.4%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 10,255,618 bp (1.0%) === Adapter 1 === Sequence: ACAGGT; Type: regular 5'; Length: 6; Trimmed: 66773 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 169 846.0 0 169 7 15658 846.0 0 15658 8 79 846.0 0 79 9 18 846.0 0 18 10 21 846.0 0 21 11 18 846.0 0 18 12 18 846.0 0 18 13 25 846.0 0 25 14 26 846.0 0 26 15 19 846.0 0 19 16 23 846.0 0 23 17 23 846.0 0 23 18 64 846.0 0 64 19 53 846.0 0 53 20 38 846.0 0 38 21 36 846.0 0 36 22 32 846.0 0 32 23 29 846.0 0 29 24 17 846.0 0 17 25 30 846.0 0 30 26 28 846.0 0 28 27 36 846.0 0 36 28 41 846.0 0 41 29 24 846.0 0 24 30 20 846.0 0 20 31 35 846.0 0 35 32 26 846.0 0 26 33 26 846.0 0 26 34 25 846.0 0 25 35 25 846.0 0 25 36 20 846.0 0 20 37 26 846.0 0 26 38 20 846.0 0 20 39 28 846.0 0 28 40 19 846.0 0 19 41 40 846.0 0 40 42 20 846.0 0 20 43 24 846.0 0 24 44 46 846.0 0 46 45 29 846.0 0 29 46 24 846.0 0 24 47 27 846.0 0 27 48 29 846.0 0 29 49 23 846.0 0 23 50 16 846.0 0 16 51 13 846.0 0 13 52 53 846.0 0 53 53 21 846.0 0 21 54 42 846.0 0 42 55 32 846.0 0 32 56 29 846.0 0 29 57 18 846.0 0 18 58 30 846.0 0 30 59 34 846.0 0 34 60 26 846.0 0 26 61 25 846.0 0 25 62 22 846.0 0 22 63 17 846.0 0 17 64 32 846.0 0 32 65 38 846.0 0 38 66 48 846.0 0 48 67 57 846.0 0 57 68 59 846.0 0 59 69 86 846.0 0 86 70 100 846.0 0 100 71 2171 846.0 0 2171 72 3079 846.0 0 3079 73 1549 846.0 0 1549 74 73 846.0 0 73 75 44 846.0 0 44 76 58 846.0 0 58 77 155 846.0 0 155 78 191 846.0 0 191 79 95 846.0 0 95 80 61 846.0 0 61 81 48 846.0 0 48 82 83 846.0 0 83 83 132 846.0 0 132 84 164 846.0 0 164 85 203 846.0 0 203 86 249 846.0 0 249 87 133 846.0 0 133 88 52 846.0 0 52 89 54 846.0 0 54 90 120 846.0 0 120 91 94 846.0 0 94 92 156 846.0 0 156 93 623 846.0 0 623 94 340 846.0 0 340 95 180 846.0 0 180 96 860 846.0 0 860 97 403 846.0 0 403 98 572 846.0 0 572 99 109 846.0 0 109 100 44 846.0 0 44 101 51 846.0 0 51 102 40 846.0 0 40 103 28 846.0 0 28 104 23 846.0 0 23 105 32 846.0 0 32 106 25 846.0 0 25 107 48 846.0 0 48 108 105 846.0 0 105 109 89 846.0 0 89 110 60 846.0 0 60 111 80 846.0 0 80 112 75 846.0 0 75 113 55 846.0 0 55 114 189 846.0 0 189 115 161 846.0 0 161 116 118 846.0 0 118 117 84 846.0 0 84 118 97 846.0 0 97 119 123 846.0 0 123 120 104 846.0 0 104 121 101 846.0 0 101 122 80 846.0 0 80 123 120 846.0 0 120 124 272 846.0 0 272 125 305 846.0 0 305 126 313 846.0 0 313 127 147 846.0 0 147 128 173 846.0 0 173 129 211 846.0 0 211 130 227 846.0 0 227 131 139 846.0 0 139 132 160 846.0 0 160 133 149 846.0 0 149 134 160 846.0 0 160 135 324 846.0 0 324 136 196 846.0 0 196 137 141 846.0 0 141 138 105 846.0 0 105 139 129 846.0 0 129 140 113 846.0 0 113 141 108 846.0 0 108 142 118 846.0 0 118 143 130 846.0 0 130 144 179 846.0 0 179 145 150 846.0 0 150 146 114 846.0 0 114 147 125 846.0 0 125 148 84 846.0 0 84 149 113 846.0 0 113 150 122 846.0 0 122 151 119 846.0 0 119 152 92 846.0 0 92 153 180 846.0 0 180 154 240 846.0 0 240 155 259 846.0 0 259 156 212 846.0 0 212 157 119 846.0 0 119 158 147 846.0 0 147 159 139 846.0 0 139 160 102 846.0 0 102 161 118 846.0 0 118 162 92 846.0 0 92 163 128 846.0 0 128 164 179 846.0 0 179 165 275 846.0 0 275 166 186 846.0 0 186 167 144 846.0 0 144 168 116 846.0 0 116 169 113 846.0 0 113 170 125 846.0 0 125 171 135 846.0 0 135 172 181 846.0 0 181 173 276 846.0 0 276 174 301 846.0 0 301 175 205 846.0 0 205 176 140 846.0 0 140 177 118 846.0 0 118 178 186 846.0 0 186 179 161 846.0 0 161 180 178 846.0 0 178 181 177 846.0 0 177 182 176 846.0 0 176 183 156 846.0 0 156 184 173 846.0 0 173 185 203 846.0 0 203 186 216 846.0 0 216 187 93 846.0 0 93 188 103 846.0 0 103 189 141 846.0 0 141 190 259 846.0 0 259 191 390 846.0 0 390 192 715 846.0 0 715 193 1026 846.0 0 1026 194 947 846.0 0 947 195 159 846.0 0 159 196 177 846.0 0 177 197 136 846.0 0 136 198 159 846.0 0 159 199 102 846.0 0 102 200 120 846.0 0 120 201 155 846.0 0 155 202 136 846.0 0 136 203 298 846.0 0 298 204 303 846.0 0 303 205 164 846.0 0 164 206 121 846.0 0 121 207 119 846.0 0 119 208 137 846.0 0 137 209 215 846.0 0 215 210 354 846.0 0 354 211 295 846.0 0 295 212 225 846.0 0 225 213 141 846.0 0 141 214 124 846.0 0 124 215 163 846.0 0 163 216 305 846.0 0 305 217 220 846.0 0 220 218 135 846.0 0 135 219 133 846.0 0 133 220 145 846.0 0 145 221 164 846.0 0 164 222 179 846.0 0 179 223 146 846.0 0 146 224 222 846.0 0 222 225 554 846.0 0 554 226 223 846.0 0 223 227 149 846.0 0 149 228 101 846.0 0 101 229 127 846.0 0 127 230 97 846.0 0 97 231 131 846.0 0 131 232 157 846.0 0 157 233 167 846.0 0 167 234 125 846.0 0 125 235 133 846.0 0 133 236 161 846.0 0 161 237 189 846.0 0 189 238 145 846.0 0 145 239 70 846.0 0 70 240 143 846.0 0 143 241 193 846.0 0 193 242 162 846.0 0 162 243 137 846.0 0 137 244 166 846.0 0 166 245 168 846.0 0 168 246 99 846.0 0 99 247 104 846.0 0 104 248 47 846.0 0 47 249 115 846.0 0 115 250 136 846.0 0 136 251 199 846.0 0 199 252 148 846.0 0 148 253 358 846.0 0 358 254 197 846.0 0 197 255 179 846.0 0 179 256 158 846.0 0 158 257 81 846.0 0 81 258 93 846.0 0 93 259 112 846.0 0 112 260 93 846.0 0 93 261 137 846.0 0 137 262 168 846.0 0 168 263 186 846.0 0 186 264 217 846.0 0 217 265 212 846.0 0 212 266 131 846.0 0 131 267 107 846.0 0 107 268 72 846.0 0 72 269 107 846.0 0 107 270 190 846.0 0 190 271 355 846.0 0 355 272 1150 846.0 0 1150 273 217 846.0 0 217 274 130 846.0 0 130 275 117 846.0 0 117 276 210 846.0 0 210 277 194 846.0 0 194 278 204 846.0 0 204 279 125 846.0 0 125 280 119 846.0 0 119 281 107 846.0 0 107 282 232 846.0 0 232 283 144 846.0 0 144 284 206 846.0 0 206 285 185 846.0 0 185 286 118 846.0 0 118 287 125 846.0 0 125 288 185 846.0 0 185 289 216 846.0 0 216 290 889 846.0 0 889 291 360 846.0 0 360 292 1086 846.0 0 1086 293 128 846.0 0 128 294 141 846.0 0 141 295 137 846.0 0 137 296 147 846.0 0 147 297 183 846.0 0 183 298 151 846.0 0 151 299 133 846.0 0 133 300 302 846.0 0 302 301 95 846.0 0 95 Finished in 1.87 s (40 us/read; 1.51 M reads/minute). === Summary === Total reads processed: 47,094 Reads with adapters: 15,789 (33.5%) Reads that were too short: 29 (0.1%) Reads written (passing filters): 15,760 (33.5%) Total basepairs processed: 10,255,618 bp Total written (filtered): 4,355,770 bp (42.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 15789 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 3 0.0 3 0 0 2 1 16 100 0.0 3 0 90 8 2 17 12319 0.0 3 12038 251 24 6 18 61 0.0 3 3 49 4 5 19 3234 0.0 3 3156 62 13 3 20 15 0.0 3 3 6 0 6 21 3 0.0 3 1 0 1 1 22 5 0.0 3 0 0 0 5 38 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 67 8 0.0 3 0 0 0 8 68 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 93 2 0.0 3 0 0 0 2 95 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 1 179 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 218 6 0.0 3 0 0 1 5 223 1 0.0 3 0 0 0 1 224 1 0.0 3 0 0 0 1 230 1 0.0 3 0 0 0 1 252 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.56 s (36 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 15,760 Reads with adapters: 1,050 (6.7%) Reads written (passing filters): 15,760 (100.0%) Total basepairs processed: 4,355,770 bp Total written (filtered): 4,352,610 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1050 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 35.0% C: 54.4% G: 7.0% T: 3.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1041 246.2 0 1041 4 8 61.6 0 8 5 1 15.4 0 1