This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACATTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 90.41 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 64,333 (1.9%) Reads that were too short: 23,704 (0.7%) Reads written (passing filters): 40,629 (1.2%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 9,396,437 bp (0.9%) === Adapter 1 === Sequence: ACATTG; Type: regular 5'; Length: 6; Trimmed: 64333 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 264 846.0 0 264 7 8877 846.0 0 8877 8 12221 846.0 0 12221 9 27 846.0 0 27 10 11 846.0 0 11 11 11 846.0 0 11 12 38 846.0 0 38 13 4 846.0 0 4 14 10 846.0 0 10 15 6 846.0 0 6 16 7 846.0 0 7 17 8 846.0 0 8 18 40 846.0 0 40 19 23 846.0 0 23 20 14 846.0 0 14 21 14 846.0 0 14 22 20 846.0 0 20 23 9 846.0 0 9 24 9 846.0 0 9 25 15 846.0 0 15 26 12 846.0 0 12 27 16 846.0 0 16 28 8 846.0 0 8 29 19 846.0 0 19 30 28 846.0 0 28 31 16 846.0 0 16 32 21 846.0 0 21 33 22 846.0 0 22 34 14 846.0 0 14 35 13 846.0 0 13 36 240 846.0 0 240 37 55 846.0 0 55 38 90 846.0 0 90 39 35 846.0 0 35 40 14 846.0 0 14 41 11 846.0 0 11 42 16 846.0 0 16 43 141 846.0 0 141 44 177 846.0 0 177 45 65 846.0 0 65 46 9 846.0 0 9 47 9 846.0 0 9 48 6 846.0 0 6 49 18 846.0 0 18 50 16 846.0 0 16 51 5 846.0 0 5 52 20 846.0 0 20 53 21 846.0 0 21 54 13 846.0 0 13 55 20 846.0 0 20 56 18 846.0 0 18 57 17 846.0 0 17 58 35 846.0 0 35 59 28 846.0 0 28 60 10 846.0 0 10 61 14 846.0 0 14 62 8 846.0 0 8 63 13 846.0 0 13 64 6 846.0 0 6 65 15 846.0 0 15 66 13 846.0 0 13 67 7 846.0 0 7 68 13 846.0 0 13 69 8 846.0 0 8 70 14 846.0 0 14 71 30 846.0 0 30 72 15 846.0 0 15 73 23 846.0 0 23 74 8 846.0 0 8 75 9 846.0 0 9 76 16 846.0 0 16 77 7 846.0 0 7 78 18 846.0 0 18 79 23 846.0 0 23 80 22 846.0 0 22 81 21 846.0 0 21 82 41 846.0 0 41 83 34 846.0 0 34 84 24 846.0 0 24 85 8 846.0 0 8 86 15 846.0 0 15 87 28 846.0 0 28 88 42 846.0 0 42 89 63 846.0 0 63 90 75 846.0 0 75 91 96 846.0 0 96 92 59 846.0 0 59 93 105 846.0 0 105 94 192 846.0 0 192 95 317 846.0 0 317 96 132 846.0 0 132 97 121 846.0 0 121 98 96 846.0 0 96 99 115 846.0 0 115 100 78 846.0 0 78 101 75 846.0 0 75 102 27 846.0 0 27 103 9 846.0 0 9 104 9 846.0 0 9 105 19 846.0 0 19 106 37 846.0 0 37 107 118 846.0 0 118 108 73 846.0 0 73 109 91 846.0 0 91 110 34 846.0 0 34 111 29 846.0 0 29 112 44 846.0 0 44 113 74 846.0 0 74 114 128 846.0 0 128 115 115 846.0 0 115 116 68 846.0 0 68 117 39 846.0 0 39 118 51 846.0 0 51 119 45 846.0 0 45 120 165 846.0 0 165 121 161 846.0 0 161 122 170 846.0 0 170 123 82 846.0 0 82 124 61 846.0 0 61 125 92 846.0 0 92 126 104 846.0 0 104 127 117 846.0 0 117 128 457 846.0 0 457 129 227 846.0 0 227 130 167 846.0 0 167 131 104 846.0 0 104 132 252 846.0 0 252 133 110 846.0 0 110 134 173 846.0 0 173 135 1124 846.0 0 1124 136 776 846.0 0 776 137 591 846.0 0 591 138 94 846.0 0 94 139 135 846.0 0 135 140 190 846.0 0 190 141 208 846.0 0 208 142 303 846.0 0 303 143 216 846.0 0 216 144 146 846.0 0 146 145 206 846.0 0 206 146 124 846.0 0 124 147 141 846.0 0 141 148 162 846.0 0 162 149 154 846.0 0 154 150 154 846.0 0 154 151 99 846.0 0 99 152 79 846.0 0 79 153 96 846.0 0 96 154 161 846.0 0 161 155 126 846.0 0 126 156 172 846.0 0 172 157 125 846.0 0 125 158 127 846.0 0 127 159 170 846.0 0 170 160 81 846.0 0 81 161 102 846.0 0 102 162 63 846.0 0 63 163 123 846.0 0 123 164 140 846.0 0 140 165 146 846.0 0 146 166 197 846.0 0 197 167 205 846.0 0 205 168 175 846.0 0 175 169 124 846.0 0 124 170 74 846.0 0 74 171 77 846.0 0 77 172 79 846.0 0 79 173 114 846.0 0 114 174 217 846.0 0 217 175 159 846.0 0 159 176 86 846.0 0 86 177 94 846.0 0 94 178 96 846.0 0 96 179 106 846.0 0 106 180 154 846.0 0 154 181 199 846.0 0 199 182 191 846.0 0 191 183 158 846.0 0 158 184 165 846.0 0 165 185 166 846.0 0 166 186 146 846.0 0 146 187 107 846.0 0 107 188 88 846.0 0 88 189 192 846.0 0 192 190 150 846.0 0 150 191 259 846.0 0 259 192 203 846.0 0 203 193 146 846.0 0 146 194 192 846.0 0 192 195 359 846.0 0 359 196 258 846.0 0 258 197 132 846.0 0 132 198 63 846.0 0 63 199 63 846.0 0 63 200 107 846.0 0 107 201 437 846.0 0 437 202 497 846.0 0 497 203 465 846.0 0 465 204 267 846.0 0 267 205 157 846.0 0 157 206 105 846.0 0 105 207 189 846.0 0 189 208 322 846.0 0 322 209 318 846.0 0 318 210 248 846.0 0 248 211 252 846.0 0 252 212 237 846.0 0 237 213 213 846.0 0 213 214 217 846.0 0 217 215 256 846.0 0 256 216 309 846.0 0 309 217 213 846.0 0 213 218 112 846.0 0 112 219 116 846.0 0 116 220 221 846.0 0 221 221 351 846.0 0 351 222 416 846.0 0 416 223 176 846.0 0 176 224 178 846.0 0 178 225 674 846.0 0 674 226 209 846.0 0 209 227 159 846.0 0 159 228 137 846.0 0 137 229 170 846.0 0 170 230 179 846.0 0 179 231 141 846.0 0 141 232 172 846.0 0 172 233 216 846.0 0 216 234 260 846.0 0 260 235 316 846.0 0 316 236 331 846.0 0 331 237 212 846.0 0 212 238 148 846.0 0 148 239 136 846.0 0 136 240 136 846.0 0 136 241 184 846.0 0 184 242 288 846.0 0 288 243 356 846.0 0 356 244 345 846.0 0 345 245 191 846.0 0 191 246 92 846.0 0 92 247 54 846.0 0 54 248 32 846.0 0 32 249 59 846.0 0 59 250 119 846.0 0 119 251 270 846.0 0 270 252 195 846.0 0 195 253 363 846.0 0 363 254 260 846.0 0 260 255 249 846.0 0 249 256 158 846.0 0 158 257 153 846.0 0 153 258 115 846.0 0 115 259 186 846.0 0 186 260 190 846.0 0 190 261 203 846.0 0 203 262 181 846.0 0 181 263 167 846.0 0 167 264 197 846.0 0 197 265 151 846.0 0 151 266 147 846.0 0 147 267 97 846.0 0 97 268 69 846.0 0 69 269 765 846.0 0 765 270 406 846.0 0 406 271 435 846.0 0 435 272 263 846.0 0 263 273 233 846.0 0 233 274 213 846.0 0 213 275 216 846.0 0 216 276 224 846.0 0 224 277 176 846.0 0 176 278 183 846.0 0 183 279 163 846.0 0 163 280 268 846.0 0 268 281 259 846.0 0 259 282 272 846.0 0 272 283 210 846.0 0 210 284 452 846.0 0 452 285 463 846.0 0 463 286 291 846.0 0 291 287 87 846.0 0 87 288 70 846.0 0 70 289 179 846.0 0 179 290 406 846.0 0 406 291 315 846.0 0 315 292 174 846.0 0 174 293 114 846.0 0 114 294 182 846.0 0 182 295 198 846.0 0 198 296 309 846.0 0 309 297 369 846.0 0 369 298 462 846.0 0 462 299 259 846.0 0 259 300 357 846.0 0 357 301 229 846.0 0 229 Finished in 1.87 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 40,629 Reads with adapters: 12,539 (30.9%) Reads that were too short: 5 (0.0%) Reads written (passing filters): 12,534 (30.8%) Total basepairs processed: 9,396,437 bp Total written (filtered): 3,413,225 bp (36.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 12539 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 1 1 18 7 0.0 3 0 0 6 0 1 19 202 0.0 3 0 199 3 20 12206 0.0 4 12070 130 5 0 1 21 21 0.0 4 4 17 22 1 0.0 4 0 0 1 23 9 0.0 4 1 0 0 7 1 27 1 0.0 4 1 35 1 0.0 4 0 0 0 0 1 40 1 0.0 4 0 0 0 0 1 81 1 0.0 4 0 0 0 0 1 103 1 0.0 4 0 0 0 0 1 127 84 0.0 4 0 0 0 0 84 135 1 0.0 4 0 0 0 0 1 146 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.42 s (34 us/read; 1.79 M reads/minute). === Summary === Total reads processed: 12,534 Reads with adapters: 1,126 (9.0%) Reads written (passing filters): 12,534 (100.0%) Total basepairs processed: 3,413,225 bp Total written (filtered): 3,215,656 bp (94.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1126 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 50.3% C: 1.5% G: 35.8% T: 12.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 195.8 0 1 4 2 49.0 0 2 9 1 0.0 0 0 1 10 42 0.0 1 0 42 11 340 0.0 1 2 338 258 11 0.0 1 11 259 474 0.0 1 465 9 260 17 0.0 1 16 1 261 6 0.0 1 6 262 5 0.0 1 5 263 1 0.0 1 0 1 264 119 0.0 1 110 9 265 1 0.0 1 1 266 1 0.0 1 1 269 78 0.0 1 76 2 270 22 0.0 1 22 271 4 0.0 1 4 273 1 0.0 1 1