This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATGGAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 89.07 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 107,089 (3.1%) Reads that were too short: 12,614 (0.4%) Reads written (passing filters): 94,475 (2.7%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 23,585,767 bp (2.3%) === Adapter 1 === Sequence: ATGGAT; Type: regular 5'; Length: 6; Trimmed: 107089 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 468 846.0 0 468 7 751 846.0 0 751 8 713 846.0 0 713 9 51279 846.0 0 51279 10 130 846.0 0 130 11 78 846.0 0 78 12 33 846.0 0 33 13 46 846.0 0 46 14 28 846.0 0 28 15 25 846.0 0 25 16 175 846.0 0 175 17 60 846.0 0 60 18 83 846.0 0 83 19 31 846.0 0 31 20 40 846.0 0 40 21 41 846.0 0 41 22 21 846.0 0 21 23 40 846.0 0 40 24 82 846.0 0 82 25 21 846.0 0 21 26 21 846.0 0 21 27 22 846.0 0 22 28 21 846.0 0 21 29 31 846.0 0 31 30 29 846.0 0 29 31 47 846.0 0 47 32 43 846.0 0 43 33 343 846.0 0 343 34 35 846.0 0 35 35 36 846.0 0 36 36 31 846.0 0 31 37 41 846.0 0 41 38 40 846.0 0 40 39 32 846.0 0 32 40 30 846.0 0 30 41 39 846.0 0 39 42 40 846.0 0 40 43 30 846.0 0 30 44 25 846.0 0 25 45 34 846.0 0 34 46 79 846.0 0 79 47 82 846.0 0 82 48 80 846.0 0 80 49 34 846.0 0 34 50 44 846.0 0 44 51 30 846.0 0 30 52 30 846.0 0 30 53 21 846.0 0 21 54 28 846.0 0 28 55 28 846.0 0 28 56 43 846.0 0 43 57 20 846.0 0 20 58 36 846.0 0 36 59 25 846.0 0 25 60 22 846.0 0 22 61 25 846.0 0 25 62 30 846.0 0 30 63 32 846.0 0 32 64 31 846.0 0 31 65 30 846.0 0 30 66 31 846.0 0 31 67 33 846.0 0 33 68 41 846.0 0 41 69 36 846.0 0 36 70 44 846.0 0 44 71 40 846.0 0 40 72 39 846.0 0 39 73 45 846.0 0 45 74 50 846.0 0 50 75 45 846.0 0 45 76 91 846.0 0 91 77 140 846.0 0 140 78 173 846.0 0 173 79 216 846.0 0 216 80 261 846.0 0 261 81 514 846.0 0 514 82 172 846.0 0 172 83 173 846.0 0 173 84 271 846.0 0 271 85 179 846.0 0 179 86 254 846.0 0 254 87 254 846.0 0 254 88 160 846.0 0 160 89 175 846.0 0 175 90 143 846.0 0 143 91 197 846.0 0 197 92 297 846.0 0 297 93 430 846.0 0 430 94 6643 846.0 0 6643 95 7131 846.0 0 7131 96 7341 846.0 0 7341 97 322 846.0 0 322 98 446 846.0 0 446 99 71 846.0 0 71 100 88 846.0 0 88 101 118 846.0 0 118 102 46 846.0 0 46 103 47 846.0 0 47 104 35 846.0 0 35 105 29 846.0 0 29 106 34 846.0 0 34 107 31 846.0 0 31 108 45 846.0 0 45 109 71 846.0 0 71 110 412 846.0 0 412 111 234 846.0 0 234 112 212 846.0 0 212 113 97 846.0 0 97 114 56 846.0 0 56 115 93 846.0 0 93 116 115 846.0 0 115 117 98 846.0 0 98 118 105 846.0 0 105 119 119 846.0 0 119 120 84 846.0 0 84 121 91 846.0 0 91 122 72 846.0 0 72 123 68 846.0 0 68 124 101 846.0 0 101 125 134 846.0 0 134 126 145 846.0 0 145 127 97 846.0 0 97 128 218 846.0 0 218 129 208 846.0 0 208 130 142 846.0 0 142 131 536 846.0 0 536 132 209 846.0 0 209 133 166 846.0 0 166 134 113 846.0 0 113 135 75 846.0 0 75 136 113 846.0 0 113 137 192 846.0 0 192 138 248 846.0 0 248 139 209 846.0 0 209 140 116 846.0 0 116 141 104 846.0 0 104 142 140 846.0 0 140 143 220 846.0 0 220 144 159 846.0 0 159 145 153 846.0 0 153 146 157 846.0 0 157 147 95 846.0 0 95 148 96 846.0 0 96 149 84 846.0 0 84 150 89 846.0 0 89 151 85 846.0 0 85 152 75 846.0 0 75 153 72 846.0 0 72 154 92 846.0 0 92 155 96 846.0 0 96 156 93 846.0 0 93 157 113 846.0 0 113 158 150 846.0 0 150 159 102 846.0 0 102 160 169 846.0 0 169 161 85 846.0 0 85 162 77 846.0 0 77 163 75 846.0 0 75 164 77 846.0 0 77 165 96 846.0 0 96 166 102 846.0 0 102 167 117 846.0 0 117 168 85 846.0 0 85 169 79 846.0 0 79 170 94 846.0 0 94 171 90 846.0 0 90 172 82 846.0 0 82 173 82 846.0 0 82 174 103 846.0 0 103 175 81 846.0 0 81 176 90 846.0 0 90 177 75 846.0 0 75 178 68 846.0 0 68 179 90 846.0 0 90 180 171 846.0 0 171 181 101 846.0 0 101 182 102 846.0 0 102 183 103 846.0 0 103 184 97 846.0 0 97 185 114 846.0 0 114 186 159 846.0 0 159 187 259 846.0 0 259 188 89 846.0 0 89 189 58 846.0 0 58 190 78 846.0 0 78 191 125 846.0 0 125 192 204 846.0 0 204 193 157 846.0 0 157 194 347 846.0 0 347 195 90 846.0 0 90 196 57 846.0 0 57 197 65 846.0 0 65 198 113 846.0 0 113 199 128 846.0 0 128 200 79 846.0 0 79 201 42 846.0 0 42 202 75 846.0 0 75 203 115 846.0 0 115 204 138 846.0 0 138 205 288 846.0 0 288 206 344 846.0 0 344 207 187 846.0 0 187 208 98 846.0 0 98 209 107 846.0 0 107 210 115 846.0 0 115 211 128 846.0 0 128 212 121 846.0 0 121 213 126 846.0 0 126 214 104 846.0 0 104 215 76 846.0 0 76 216 101 846.0 0 101 217 112 846.0 0 112 218 121 846.0 0 121 219 119 846.0 0 119 220 107 846.0 0 107 221 83 846.0 0 83 222 78 846.0 0 78 223 112 846.0 0 112 224 107 846.0 0 107 225 67 846.0 0 67 226 77 846.0 0 77 227 97 846.0 0 97 228 127 846.0 0 127 229 119 846.0 0 119 230 81 846.0 0 81 231 77 846.0 0 77 232 93 846.0 0 93 233 94 846.0 0 94 234 79 846.0 0 79 235 106 846.0 0 106 236 171 846.0 0 171 237 100 846.0 0 100 238 143 846.0 0 143 239 174 846.0 0 174 240 141 846.0 0 141 241 71 846.0 0 71 242 73 846.0 0 73 243 86 846.0 0 86 244 96 846.0 0 96 245 109 846.0 0 109 246 103 846.0 0 103 247 133 846.0 0 133 248 64 846.0 0 64 249 59 846.0 0 59 250 43 846.0 0 43 251 64 846.0 0 64 252 95 846.0 0 95 253 123 846.0 0 123 254 81 846.0 0 81 255 98 846.0 0 98 256 91 846.0 0 91 257 204 846.0 0 204 258 103 846.0 0 103 259 65 846.0 0 65 260 49 846.0 0 49 261 107 846.0 0 107 262 104 846.0 0 104 263 98 846.0 0 98 264 118 846.0 0 118 265 112 846.0 0 112 266 144 846.0 0 144 267 129 846.0 0 129 268 116 846.0 0 116 269 59 846.0 0 59 270 54 846.0 0 54 271 207 846.0 0 207 272 998 846.0 0 998 273 220 846.0 0 220 274 123 846.0 0 123 275 94 846.0 0 94 276 67 846.0 0 67 277 58 846.0 0 58 278 92 846.0 0 92 279 141 846.0 0 141 280 142 846.0 0 142 281 65 846.0 0 65 282 42 846.0 0 42 283 116 846.0 0 116 284 87 846.0 0 87 285 103 846.0 0 103 286 155 846.0 0 155 287 149 846.0 0 149 288 219 846.0 0 219 289 105 846.0 0 105 290 113 846.0 0 113 291 108 846.0 0 108 292 390 846.0 0 390 293 173 846.0 0 173 294 89 846.0 0 89 295 62 846.0 0 62 296 89 846.0 0 89 297 100 846.0 0 100 298 488 846.0 0 488 299 116 846.0 0 116 300 110 846.0 0 110 301 34 846.0 0 34 Finished in 4.07 s (43 us/read; 1.39 M reads/minute). === Summary === Total reads processed: 94,475 Reads with adapters: 52,957 (56.1%) Reads that were too short: 17 (0.0%) Reads written (passing filters): 52,940 (56.0%) Total basepairs processed: 23,585,767 bp Total written (filtered): 14,556,282 bp (61.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 52957 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 16 0.0 3 0 0 16 16 511 0.0 3 0 492 14 5 17 52112 0.0 3 50791 1188 109 24 18 97 0.0 3 3 80 8 6 19 26 0.0 3 0 1 17 8 20 140 0.0 3 103 8 0 29 30 7 0.0 3 0 0 0 7 33 2 0.0 3 0 0 0 2 34 1 0.0 3 1 41 1 0.0 3 0 0 0 1 44 2 0.0 3 0 0 0 2 49 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 92 5 0.0 3 0 0 0 5 95 2 0.0 3 0 0 0 2 104 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 116 2 0.0 3 0 0 0 2 118 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 142 3 0.0 3 0 0 0 3 143 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 200 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 259 1 0.0 3 0 0 0 1 266 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.88 s (36 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 52,940 Reads with adapters: 2,311 (4.4%) Reads written (passing filters): 52,940 (100.0%) Total basepairs processed: 14,556,282 bp Total written (filtered): 14,549,315 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 2311 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 63.0% C: 8.9% G: 25.0% T: 3.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2280 827.2 0 2280 4 29 206.8 0 29 5 1 51.7 0 1 6 1 12.9 0 1