This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGCGAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 90.55 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 144,377 (4.2%) Reads that were too short: 6,235 (0.2%) Reads written (passing filters): 138,142 (4.0%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 38,970,163 bp (3.7%) === Adapter 1 === Sequence: CGCGAT; Type: regular 5'; Length: 6; Trimmed: 144377 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1157 846.0 0 1157 7 59297 846.0 0 59297 8 750 846.0 0 750 9 65683 846.0 0 65683 10 80 846.0 0 80 11 31 846.0 0 31 12 22 846.0 0 22 13 27 846.0 0 27 14 28 846.0 0 28 15 31 846.0 0 31 16 36 846.0 0 36 17 27 846.0 0 27 18 29 846.0 0 29 19 23 846.0 0 23 20 31 846.0 0 31 21 30 846.0 0 30 22 33 846.0 0 33 23 28 846.0 0 28 24 35 846.0 0 35 25 22 846.0 0 22 26 34 846.0 0 34 27 27 846.0 0 27 28 23 846.0 0 23 29 26 846.0 0 26 30 29 846.0 0 29 31 28 846.0 0 28 32 31 846.0 0 31 33 18 846.0 0 18 34 21 846.0 0 21 35 27 846.0 0 27 36 27 846.0 0 27 37 28 846.0 0 28 38 27 846.0 0 27 39 27 846.0 0 27 40 20 846.0 0 20 41 26 846.0 0 26 42 40 846.0 0 40 43 52 846.0 0 52 44 56 846.0 0 56 45 21 846.0 0 21 46 17 846.0 0 17 47 21 846.0 0 21 48 33 846.0 0 33 49 25 846.0 0 25 50 21 846.0 0 21 51 18 846.0 0 18 52 27 846.0 0 27 53 19 846.0 0 19 54 28 846.0 0 28 55 59 846.0 0 59 56 31 846.0 0 31 57 42 846.0 0 42 58 39 846.0 0 39 59 26 846.0 0 26 60 15 846.0 0 15 61 31 846.0 0 31 62 23 846.0 0 23 63 25 846.0 0 25 64 24 846.0 0 24 65 41 846.0 0 41 66 34 846.0 0 34 67 13 846.0 0 13 68 15 846.0 0 15 69 32 846.0 0 32 70 19 846.0 0 19 71 16 846.0 0 16 72 24 846.0 0 24 73 27 846.0 0 27 74 22 846.0 0 22 75 32 846.0 0 32 76 17 846.0 0 17 77 23 846.0 0 23 78 13 846.0 0 13 79 23 846.0 0 23 80 26 846.0 0 26 81 42 846.0 0 42 82 22 846.0 0 22 83 13 846.0 0 13 84 17 846.0 0 17 85 13 846.0 0 13 86 22 846.0 0 22 87 34 846.0 0 34 88 21 846.0 0 21 89 27 846.0 0 27 90 17 846.0 0 17 91 29 846.0 0 29 92 208 846.0 0 208 93 107 846.0 0 107 94 184 846.0 0 184 95 34 846.0 0 34 96 52 846.0 0 52 97 36 846.0 0 36 98 19 846.0 0 19 99 21 846.0 0 21 100 30 846.0 0 30 101 28 846.0 0 28 102 13 846.0 0 13 103 18 846.0 0 18 104 17 846.0 0 17 105 20 846.0 0 20 106 17 846.0 0 17 107 34 846.0 0 34 108 25 846.0 0 25 109 26 846.0 0 26 110 34 846.0 0 34 111 23 846.0 0 23 112 31 846.0 0 31 113 35 846.0 0 35 114 22 846.0 0 22 115 27 846.0 0 27 116 26 846.0 0 26 117 26 846.0 0 26 118 27 846.0 0 27 119 44 846.0 0 44 120 32 846.0 0 32 121 30 846.0 0 30 122 25 846.0 0 25 123 106 846.0 0 106 124 227 846.0 0 227 125 477 846.0 0 477 126 28 846.0 0 28 127 26 846.0 0 26 128 18 846.0 0 18 129 29 846.0 0 29 130 62 846.0 0 62 131 49 846.0 0 49 132 26 846.0 0 26 133 31 846.0 0 31 134 29 846.0 0 29 135 25 846.0 0 25 136 34 846.0 0 34 137 25 846.0 0 25 138 35 846.0 0 35 139 23 846.0 0 23 140 22 846.0 0 22 141 32 846.0 0 32 142 33 846.0 0 33 143 36 846.0 0 36 144 25 846.0 0 25 145 28 846.0 0 28 146 36 846.0 0 36 147 163 846.0 0 163 148 29 846.0 0 29 149 34 846.0 0 34 150 22 846.0 0 22 151 20 846.0 0 20 152 33 846.0 0 33 153 23 846.0 0 23 154 37 846.0 0 37 155 21 846.0 0 21 156 25 846.0 0 25 157 27 846.0 0 27 158 67 846.0 0 67 159 32 846.0 0 32 160 32 846.0 0 32 161 33 846.0 0 33 162 35 846.0 0 35 163 34 846.0 0 34 164 44 846.0 0 44 165 38 846.0 0 38 166 38 846.0 0 38 167 48 846.0 0 48 168 43 846.0 0 43 169 28 846.0 0 28 170 40 846.0 0 40 171 37 846.0 0 37 172 47 846.0 0 47 173 328 846.0 0 328 174 137 846.0 0 137 175 82 846.0 0 82 176 87 846.0 0 87 177 96 846.0 0 96 178 198 846.0 0 198 179 187 846.0 0 187 180 221 846.0 0 221 181 107 846.0 0 107 182 80 846.0 0 80 183 48 846.0 0 48 184 54 846.0 0 54 185 67 846.0 0 67 186 75 846.0 0 75 187 33 846.0 0 33 188 41 846.0 0 41 189 30 846.0 0 30 190 35 846.0 0 35 191 46 846.0 0 46 192 74 846.0 0 74 193 124 846.0 0 124 194 221 846.0 0 221 195 83 846.0 0 83 196 49 846.0 0 49 197 42 846.0 0 42 198 427 846.0 0 427 199 1548 846.0 0 1548 200 589 846.0 0 589 201 124 846.0 0 124 202 49 846.0 0 49 203 25 846.0 0 25 204 57 846.0 0 57 205 30 846.0 0 30 206 144 846.0 0 144 207 38 846.0 0 38 208 57 846.0 0 57 209 47 846.0 0 47 210 23 846.0 0 23 211 30 846.0 0 30 212 29 846.0 0 29 213 26 846.0 0 26 214 41 846.0 0 41 215 81 846.0 0 81 216 62 846.0 0 62 217 39 846.0 0 39 218 43 846.0 0 43 219 136 846.0 0 136 220 118 846.0 0 118 221 76 846.0 0 76 222 118 846.0 0 118 223 92 846.0 0 92 224 95 846.0 0 95 225 51 846.0 0 51 226 46 846.0 0 46 227 61 846.0 0 61 228 54 846.0 0 54 229 47 846.0 0 47 230 40 846.0 0 40 231 40 846.0 0 40 232 38 846.0 0 38 233 28 846.0 0 28 234 28 846.0 0 28 235 31 846.0 0 31 236 44 846.0 0 44 237 45 846.0 0 45 238 141 846.0 0 141 239 178 846.0 0 178 240 783 846.0 0 783 241 398 846.0 0 398 242 168 846.0 0 168 243 53 846.0 0 53 244 31 846.0 0 31 245 41 846.0 0 41 246 55 846.0 0 55 247 68 846.0 0 68 248 113 846.0 0 113 249 84 846.0 0 84 250 39 846.0 0 39 251 26 846.0 0 26 252 23 846.0 0 23 253 39 846.0 0 39 254 39 846.0 0 39 255 38 846.0 0 38 256 73 846.0 0 73 257 63 846.0 0 63 258 70 846.0 0 70 259 45 846.0 0 45 260 40 846.0 0 40 261 32 846.0 0 32 262 30 846.0 0 30 263 32 846.0 0 32 264 40 846.0 0 40 265 32 846.0 0 32 266 30 846.0 0 30 267 47 846.0 0 47 268 63 846.0 0 63 269 47 846.0 0 47 270 15 846.0 0 15 271 24 846.0 0 24 272 25 846.0 0 25 273 26 846.0 0 26 274 41 846.0 0 41 275 55 846.0 0 55 276 41 846.0 0 41 277 31 846.0 0 31 278 44 846.0 0 44 279 35 846.0 0 35 280 62 846.0 0 62 281 29 846.0 0 29 282 25 846.0 0 25 283 35 846.0 0 35 284 38 846.0 0 38 285 37 846.0 0 37 286 67 846.0 0 67 287 92 846.0 0 92 288 89 846.0 0 89 289 58 846.0 0 58 290 66 846.0 0 66 291 38 846.0 0 38 292 50 846.0 0 50 293 45 846.0 0 45 294 50 846.0 0 50 295 31 846.0 0 31 296 22 846.0 0 22 297 18 846.0 0 18 298 12 846.0 0 12 299 20 846.0 0 20 300 31 846.0 0 31 301 13 846.0 0 13 Finished in 6.95 s (50 us/read; 1.19 M reads/minute). === Summary === Total reads processed: 138,142 Reads with adapters: 60,361 (43.7%) Reads that were too short: 11 (0.0%) Reads written (passing filters): 60,350 (43.7%) Total basepairs processed: 38,970,163 bp Total written (filtered): 16,419,097 bp (42.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 60361 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 5 0.0 3 0 0 0 1 4 18 27 0.0 3 0 0 25 2 19 718 0.0 3 0 703 14 1 20 58398 0.0 4 57589 768 31 7 3 21 94 0.0 4 16 77 1 22 8 0.0 4 1 1 5 0 1 23 15 0.0 4 0 0 0 15 24 3 0.0 4 0 0 0 0 3 53 1 0.0 4 0 0 0 0 1 54 1 0.0 4 0 0 0 0 1 86 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 126 15 0.0 4 0 0 0 0 15 127 1064 0.0 4 0 0 0 0 1064 128 1 0.0 4 0 0 0 0 1 164 1 0.0 4 0 0 0 0 1 194 3 0.0 4 0 0 0 0 3 228 2 0.0 4 0 0 0 0 2 230 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.04 s (34 us/read; 1.77 M reads/minute). === Summary === Total reads processed: 60,350 Reads with adapters: 1,509 (2.5%) Reads written (passing filters): 60,350 (100.0%) Total basepairs processed: 16,419,097 bp Total written (filtered): 16,331,328 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1509 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 10.5% C: 2.7% G: 78.8% T: 8.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 18 943.0 0 18 4 2 235.7 0 2 9 1 0.2 0 0 1 10 2 0.1 1 0 2 11 1169 0.0 1 3 1166 12 32 0.0 1 0 32 14 1 0.0 1 0 1 15 2 0.0 1 0 2 120 1 0.0 1 1 152 2 0.0 1 0 2 210 2 0.0 1 2 215 1 0.0 1 1 260 1 0.0 1 1 261 122 0.0 1 114 8 262 6 0.0 1 6 264 2 0.0 1 2 265 28 0.0 1 27 1 266 1 0.0 1 1 269 1 0.0 1 1 271 106 0.0 1 102 4 272 8 0.0 1 8 273 1 0.0 1 1