This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTCTGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 89.84 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 195,758 (5.6%) Reads that were too short: 12,300 (0.4%) Reads written (passing filters): 183,458 (5.3%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 31,783,326 bp (3.0%) === Adapter 1 === Sequence: CTCTGC; Type: regular 5'; Length: 6; Trimmed: 195758 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 146 846.0 0 146 7 6919 846.0 0 6919 8 18 846.0 0 18 9 47 846.0 0 47 10 23 846.0 0 23 11 28 846.0 0 28 12 24 846.0 0 24 13 17 846.0 0 17 14 281 846.0 0 281 15 38 846.0 0 38 16 25 846.0 0 25 17 30 846.0 0 30 18 20 846.0 0 20 19 22 846.0 0 22 20 27 846.0 0 27 21 24 846.0 0 24 22 44 846.0 0 44 23 18 846.0 0 18 24 60 846.0 0 60 25 40 846.0 0 40 26 18 846.0 0 18 27 23 846.0 0 23 28 22 846.0 0 22 29 17 846.0 0 17 30 23 846.0 0 23 31 32 846.0 0 32 32 22 846.0 0 22 33 29 846.0 0 29 34 28 846.0 0 28 35 27 846.0 0 27 36 23 846.0 0 23 37 15 846.0 0 15 38 26 846.0 0 26 39 33 846.0 0 33 40 30 846.0 0 30 41 45 846.0 0 45 42 38 846.0 0 38 43 26 846.0 0 26 44 21 846.0 0 21 45 24 846.0 0 24 46 39 846.0 0 39 47 25 846.0 0 25 48 36 846.0 0 36 49 26 846.0 0 26 50 28 846.0 0 28 51 25 846.0 0 25 52 29 846.0 0 29 53 24 846.0 0 24 54 30 846.0 0 30 55 21 846.0 0 21 56 51 846.0 0 51 57 44 846.0 0 44 58 83 846.0 0 83 59 63 846.0 0 63 60 75 846.0 0 75 61 51 846.0 0 51 62 27 846.0 0 27 63 26 846.0 0 26 64 22 846.0 0 22 65 21 846.0 0 21 66 14 846.0 0 14 67 24 846.0 0 24 68 23 846.0 0 23 69 24 846.0 0 24 70 31 846.0 0 31 71 12 846.0 0 12 72 40 846.0 0 40 73 38 846.0 0 38 74 32 846.0 0 32 75 29 846.0 0 29 76 25 846.0 0 25 77 87 846.0 0 87 78 35 846.0 0 35 79 41 846.0 0 41 80 22 846.0 0 22 81 39 846.0 0 39 82 47 846.0 0 47 83 91 846.0 0 91 84 308 846.0 0 308 85 66 846.0 0 66 86 41 846.0 0 41 87 104 846.0 0 104 88 32 846.0 0 32 89 70 846.0 0 70 90 95 846.0 0 95 91 60 846.0 0 60 92 145 846.0 0 145 93 189 846.0 0 189 94 228 846.0 0 228 95 47 846.0 0 47 96 89 846.0 0 89 97 168 846.0 0 168 98 72 846.0 0 72 99 109 846.0 0 109 100 85 846.0 0 85 101 99 846.0 0 99 102 146 846.0 0 146 103 62 846.0 0 62 104 63 846.0 0 63 105 52 846.0 0 52 106 108 846.0 0 108 107 144 846.0 0 144 108 225 846.0 0 225 109 214 846.0 0 214 110 402 846.0 0 402 111 480 846.0 0 480 112 726 846.0 0 726 113 1208 846.0 0 1208 114 40543 846.0 0 40543 115 14792 846.0 0 14792 116 21036 846.0 0 21036 117 249 846.0 0 249 118 103 846.0 0 103 119 34 846.0 0 34 120 47 846.0 0 47 121 42 846.0 0 42 122 36 846.0 0 36 123 61 846.0 0 61 124 37 846.0 0 37 125 39 846.0 0 39 126 129 846.0 0 129 127 108 846.0 0 108 128 273 846.0 0 273 129 248 846.0 0 248 130 354 846.0 0 354 131 119 846.0 0 119 132 60 846.0 0 60 133 53 846.0 0 53 134 56 846.0 0 56 135 119 846.0 0 119 136 143 846.0 0 143 137 226 846.0 0 226 138 99 846.0 0 99 139 82 846.0 0 82 140 270 846.0 0 270 141 534 846.0 0 534 142 574 846.0 0 574 143 324 846.0 0 324 144 217 846.0 0 217 145 319 846.0 0 319 146 663 846.0 0 663 147 19822 846.0 0 19822 148 1270 846.0 0 1270 149 8663 846.0 0 8663 150 532 846.0 0 532 151 503 846.0 0 503 152 531 846.0 0 531 153 5296 846.0 0 5296 154 41549 846.0 0 41549 155 2709 846.0 0 2709 156 310 846.0 0 310 157 160 846.0 0 160 158 136 846.0 0 136 159 50 846.0 0 50 160 49 846.0 0 49 161 88 846.0 0 88 162 122 846.0 0 122 163 138 846.0 0 138 164 111 846.0 0 111 165 76 846.0 0 76 166 61 846.0 0 61 167 87 846.0 0 87 168 224 846.0 0 224 169 558 846.0 0 558 170 181 846.0 0 181 171 128 846.0 0 128 172 52 846.0 0 52 173 47 846.0 0 47 174 57 846.0 0 57 175 69 846.0 0 69 176 103 846.0 0 103 177 65 846.0 0 65 178 75 846.0 0 75 179 47 846.0 0 47 180 56 846.0 0 56 181 107 846.0 0 107 182 142 846.0 0 142 183 408 846.0 0 408 184 142 846.0 0 142 185 204 846.0 0 204 186 103 846.0 0 103 187 81 846.0 0 81 188 41 846.0 0 41 189 67 846.0 0 67 190 86 846.0 0 86 191 62 846.0 0 62 192 82 846.0 0 82 193 41 846.0 0 41 194 37 846.0 0 37 195 39 846.0 0 39 196 53 846.0 0 53 197 42 846.0 0 42 198 55 846.0 0 55 199 39 846.0 0 39 200 45 846.0 0 45 201 58 846.0 0 58 202 382 846.0 0 382 203 234 846.0 0 234 204 281 846.0 0 281 205 73 846.0 0 73 206 42 846.0 0 42 207 39 846.0 0 39 208 35 846.0 0 35 209 64 846.0 0 64 210 52 846.0 0 52 211 78 846.0 0 78 212 51 846.0 0 51 213 55 846.0 0 55 214 77 846.0 0 77 215 112 846.0 0 112 216 224 846.0 0 224 217 366 846.0 0 366 218 144 846.0 0 144 219 37 846.0 0 37 220 46 846.0 0 46 221 49 846.0 0 49 222 47 846.0 0 47 223 63 846.0 0 63 224 66 846.0 0 66 225 96 846.0 0 96 226 128 846.0 0 128 227 120 846.0 0 120 228 164 846.0 0 164 229 37 846.0 0 37 230 46 846.0 0 46 231 47 846.0 0 47 232 64 846.0 0 64 233 79 846.0 0 79 234 94 846.0 0 94 235 93 846.0 0 93 236 113 846.0 0 113 237 67 846.0 0 67 238 57 846.0 0 57 239 33 846.0 0 33 240 46 846.0 0 46 241 40 846.0 0 40 242 22 846.0 0 22 243 55 846.0 0 55 244 100 846.0 0 100 245 118 846.0 0 118 246 105 846.0 0 105 247 49 846.0 0 49 248 45 846.0 0 45 249 503 846.0 0 503 250 46 846.0 0 46 251 100 846.0 0 100 252 75 846.0 0 75 253 96 846.0 0 96 254 152 846.0 0 152 255 136 846.0 0 136 256 70 846.0 0 70 257 117 846.0 0 117 258 42 846.0 0 42 259 44 846.0 0 44 260 51 846.0 0 51 261 43 846.0 0 43 262 38 846.0 0 38 263 55 846.0 0 55 264 61 846.0 0 61 265 82 846.0 0 82 266 78 846.0 0 78 267 79 846.0 0 79 268 65 846.0 0 65 269 50 846.0 0 50 270 84 846.0 0 84 271 101 846.0 0 101 272 63 846.0 0 63 273 204 846.0 0 204 274 171 846.0 0 171 275 251 846.0 0 251 276 106 846.0 0 106 277 75 846.0 0 75 278 53 846.0 0 53 279 93 846.0 0 93 280 375 846.0 0 375 281 1499 846.0 0 1499 282 433 846.0 0 433 283 503 846.0 0 503 284 67 846.0 0 67 285 89 846.0 0 89 286 53 846.0 0 53 287 37 846.0 0 37 288 32 846.0 0 32 289 58 846.0 0 58 290 152 846.0 0 152 291 481 846.0 0 481 292 227 846.0 0 227 293 118 846.0 0 118 294 135 846.0 0 135 295 89 846.0 0 89 296 104 846.0 0 104 297 105 846.0 0 105 298 63 846.0 0 63 299 32 846.0 0 32 300 13 846.0 0 13 301 16 846.0 0 16 Finished in 6.14 s (33 us/read; 1.79 M reads/minute). === Summary === Total reads processed: 183,458 Reads with adapters: 7,115 (3.9%) Reads that were too short: 99 (0.1%) Reads written (passing filters): 7,016 (3.8%) Total basepairs processed: 31,783,326 bp Total written (filtered): 1,933,870 bp (6.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 7115 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 1 16 51 0.0 3 0 50 0 1 17 6864 0.0 3 6748 100 10 6 18 27 0.0 3 1 21 4 1 19 6 0.0 3 0 0 3 3 20 6 0.0 3 0 0 0 6 22 3 0.0 3 0 0 1 2 23 1 0.0 3 0 0 0 1 24 1 0.0 3 1 31 2 0.0 3 0 0 0 2 32 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 34 2 0.0 3 0 0 0 2 44 1 0.0 3 0 0 0 1 49 2 0.0 3 0 0 0 2 50 1 0.0 3 0 0 0 1 51 5 0.0 3 0 0 0 5 52 1 0.0 3 0 0 0 1 54 3 0.0 3 0 0 0 3 57 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 60 3 0.0 3 0 0 0 3 64 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 85 24 0.0 3 0 0 0 24 86 2 0.0 3 0 0 1 1 88 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 98 2 0.0 3 0 0 0 2 99 1 0.0 3 0 0 0 1 110 3 0.0 3 0 0 0 3 121 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 129 3 0.0 3 0 0 0 3 134 1 0.0 3 0 0 0 1 135 14 0.0 3 0 0 0 14 136 8 0.0 3 0 0 0 8 141 1 0.0 3 0 0 0 1 142 6 0.0 3 0 0 0 6 145 3 0.0 3 0 0 0 3 148 13 0.0 3 0 0 2 11 152 2 0.0 3 0 0 0 2 153 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 174 9 0.0 3 0 0 0 9 175 15 0.0 3 0 0 0 15 178 2 0.0 3 0 0 0 2 180 1 0.0 3 0 0 0 1 181 5 0.0 3 0 0 0 5 185 1 0.0 3 0 0 0 1 251 1 0.0 3 0 0 0 1 269 1 0.0 3 0 0 0 1 273 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.26 s (37 us/read; 1.62 M reads/minute). === Summary === Total reads processed: 7,016 Reads with adapters: 493 (7.0%) Reads written (passing filters): 7,016 (100.0%) Total basepairs processed: 1,933,870 bp Total written (filtered): 1,932,346 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 493 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 50.9% C: 25.8% G: 15.6% T: 7.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 461 109.6 0 461 4 25 27.4 0 25 5 5 6.9 0 5 8 2 0.1 0 2