This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGTGGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 88.10 s (25 us/read; 2.36 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 33,344 (1.0%) Reads that were too short: 23,603 (0.7%) Reads written (passing filters): 9,741 (0.3%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 2,232,793 bp (0.2%) === Adapter 1 === Sequence: TGTGGC; Type: regular 5'; Length: 6; Trimmed: 33344 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 51 846.0 0 51 7 54 846.0 0 54 8 61 846.0 0 61 9 3216 846.0 0 3216 10 28 846.0 0 28 11 33 846.0 0 33 12 21 846.0 0 21 13 18 846.0 0 18 14 18 846.0 0 18 15 16 846.0 0 16 16 20 846.0 0 20 17 37 846.0 0 37 18 38 846.0 0 38 19 131 846.0 0 131 20 22 846.0 0 22 21 101 846.0 0 101 22 19 846.0 0 19 23 22 846.0 0 22 24 24 846.0 0 24 25 21 846.0 0 21 26 23 846.0 0 23 27 24 846.0 0 24 28 22 846.0 0 22 29 15 846.0 0 15 30 54 846.0 0 54 31 14 846.0 0 14 32 41 846.0 0 41 33 32 846.0 0 32 34 42 846.0 0 42 35 17 846.0 0 17 36 15 846.0 0 15 37 24 846.0 0 24 38 26 846.0 0 26 39 28 846.0 0 28 40 30 846.0 0 30 41 30 846.0 0 30 42 22 846.0 0 22 43 22 846.0 0 22 44 24 846.0 0 24 45 25 846.0 0 25 46 39 846.0 0 39 47 231 846.0 0 231 48 29 846.0 0 29 49 23 846.0 0 23 50 15 846.0 0 15 51 24 846.0 0 24 52 18 846.0 0 18 53 66 846.0 0 66 54 27 846.0 0 27 55 25 846.0 0 25 56 12 846.0 0 12 57 15 846.0 0 15 58 24 846.0 0 24 59 58 846.0 0 58 60 24 846.0 0 24 61 31 846.0 0 31 62 28 846.0 0 28 63 26 846.0 0 26 64 22 846.0 0 22 65 10 846.0 0 10 66 32 846.0 0 32 67 23 846.0 0 23 68 28 846.0 0 28 69 24 846.0 0 24 70 33 846.0 0 33 71 101 846.0 0 101 72 23 846.0 0 23 73 84 846.0 0 84 74 25 846.0 0 25 75 13 846.0 0 13 76 14 846.0 0 14 77 14 846.0 0 14 78 25 846.0 0 25 79 27 846.0 0 27 80 29 846.0 0 29 81 24 846.0 0 24 82 24 846.0 0 24 83 20 846.0 0 20 84 31 846.0 0 31 85 28 846.0 0 28 86 34 846.0 0 34 87 21 846.0 0 21 88 16 846.0 0 16 89 25 846.0 0 25 90 23 846.0 0 23 91 17 846.0 0 17 92 42 846.0 0 42 93 48 846.0 0 48 94 34 846.0 0 34 95 35 846.0 0 35 96 26 846.0 0 26 97 29 846.0 0 29 98 26 846.0 0 26 99 34 846.0 0 34 100 24 846.0 0 24 101 17 846.0 0 17 102 23 846.0 0 23 103 24 846.0 0 24 104 13 846.0 0 13 105 15 846.0 0 15 106 28 846.0 0 28 107 22 846.0 0 22 108 21 846.0 0 21 109 15 846.0 0 15 110 24 846.0 0 24 111 17 846.0 0 17 112 18 846.0 0 18 113 22 846.0 0 22 114 12 846.0 0 12 115 17 846.0 0 17 116 28 846.0 0 28 117 42 846.0 0 42 118 24 846.0 0 24 119 11 846.0 0 11 120 256 846.0 0 256 121 27 846.0 0 27 122 18 846.0 0 18 123 39 846.0 0 39 124 13 846.0 0 13 125 20 846.0 0 20 126 21 846.0 0 21 127 18 846.0 0 18 128 23 846.0 0 23 129 43 846.0 0 43 130 186 846.0 0 186 131 87 846.0 0 87 132 310 846.0 0 310 133 59 846.0 0 59 134 15 846.0 0 15 135 27 846.0 0 27 136 27 846.0 0 27 137 110 846.0 0 110 138 53 846.0 0 53 139 27 846.0 0 27 140 25 846.0 0 25 141 21 846.0 0 21 142 23 846.0 0 23 143 23 846.0 0 23 144 26 846.0 0 26 145 15 846.0 0 15 146 20 846.0 0 20 147 23 846.0 0 23 148 14 846.0 0 14 149 21 846.0 0 21 150 22 846.0 0 22 151 29 846.0 0 29 152 18 846.0 0 18 153 44 846.0 0 44 154 27 846.0 0 27 155 21 846.0 0 21 156 33 846.0 0 33 157 25 846.0 0 25 158 24 846.0 0 24 159 21 846.0 0 21 160 16 846.0 0 16 161 17 846.0 0 17 162 19 846.0 0 19 163 24 846.0 0 24 164 20 846.0 0 20 165 32 846.0 0 32 166 36 846.0 0 36 167 22 846.0 0 22 168 27 846.0 0 27 169 29 846.0 0 29 170 29 846.0 0 29 171 19 846.0 0 19 172 14 846.0 0 14 173 19 846.0 0 19 174 21 846.0 0 21 175 22 846.0 0 22 176 16 846.0 0 16 177 25 846.0 0 25 178 25 846.0 0 25 179 25 846.0 0 25 180 19 846.0 0 19 181 20 846.0 0 20 182 19 846.0 0 19 183 20 846.0 0 20 184 33 846.0 0 33 185 34 846.0 0 34 186 74 846.0 0 74 187 59 846.0 0 59 188 66 846.0 0 66 189 29 846.0 0 29 190 30 846.0 0 30 191 33 846.0 0 33 192 21 846.0 0 21 193 49 846.0 0 49 194 47 846.0 0 47 195 56 846.0 0 56 196 21 846.0 0 21 197 28 846.0 0 28 198 25 846.0 0 25 199 16 846.0 0 16 200 24 846.0 0 24 201 30 846.0 0 30 202 30 846.0 0 30 203 111 846.0 0 111 204 46 846.0 0 46 205 49 846.0 0 49 206 24 846.0 0 24 207 46 846.0 0 46 208 31 846.0 0 31 209 15 846.0 0 15 210 19 846.0 0 19 211 52 846.0 0 52 212 171 846.0 0 171 213 169 846.0 0 169 214 154 846.0 0 154 215 37 846.0 0 37 216 111 846.0 0 111 217 39 846.0 0 39 218 91 846.0 0 91 219 190 846.0 0 190 220 37 846.0 0 37 221 20 846.0 0 20 222 17 846.0 0 17 223 28 846.0 0 28 224 26 846.0 0 26 225 26 846.0 0 26 226 46 846.0 0 46 227 22 846.0 0 22 228 30 846.0 0 30 229 42 846.0 0 42 230 35 846.0 0 35 231 28 846.0 0 28 232 58 846.0 0 58 233 92 846.0 0 92 234 53 846.0 0 53 235 93 846.0 0 93 236 117 846.0 0 117 237 86 846.0 0 86 238 60 846.0 0 60 239 47 846.0 0 47 240 24 846.0 0 24 241 51 846.0 0 51 242 30 846.0 0 30 243 336 846.0 0 336 244 122 846.0 0 122 245 243 846.0 0 243 246 83 846.0 0 83 247 54 846.0 0 54 248 58 846.0 0 58 249 30 846.0 0 30 250 41 846.0 0 41 251 40 846.0 0 40 252 39 846.0 0 39 253 46 846.0 0 46 254 57 846.0 0 57 255 84 846.0 0 84 256 248 846.0 0 248 257 93 846.0 0 93 258 67 846.0 0 67 259 52 846.0 0 52 260 117 846.0 0 117 261 225 846.0 0 225 262 524 846.0 0 524 263 62 846.0 0 62 264 38 846.0 0 38 265 81 846.0 0 81 266 32 846.0 0 32 267 41 846.0 0 41 268 52 846.0 0 52 269 36 846.0 0 36 270 30 846.0 0 30 271 44 846.0 0 44 272 104 846.0 0 104 273 199 846.0 0 199 274 70 846.0 0 70 275 98 846.0 0 98 276 130 846.0 0 130 277 83 846.0 0 83 278 77 846.0 0 77 279 171 846.0 0 171 280 90 846.0 0 90 281 171 846.0 0 171 282 135 846.0 0 135 283 184 846.0 0 184 284 183 846.0 0 183 285 221 846.0 0 221 286 2178 846.0 0 2178 287 4256 846.0 0 4256 288 9320 846.0 0 9320 289 74 846.0 0 74 290 23 846.0 0 23 291 33 846.0 0 33 292 26 846.0 0 26 293 28 846.0 0 28 294 23 846.0 0 23 295 23 846.0 0 23 296 60 846.0 0 60 297 58 846.0 0 58 298 99 846.0 0 99 299 19 846.0 0 19 300 23 846.0 0 23 301 16 846.0 0 16 Finished in 0.41 s (42 us/read; 1.43 M reads/minute). === Summary === Total reads processed: 9,741 Reads with adapters: 3,316 (34.0%) Reads that were too short: 10 (0.1%) Reads written (passing filters): 3,306 (33.9%) Total basepairs processed: 2,232,793 bp Total written (filtered): 907,041 bp (40.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 3316 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 25 0.0 3 0 24 1 17 3247 0.0 3 3139 93 12 3 18 11 0.0 3 0 8 2 1 19 4 0.0 3 0 0 1 3 20 2 0.0 3 1 0 0 1 32 1 0.0 3 0 0 0 1 44 7 0.0 3 0 0 0 7 63 3 0.0 3 0 0 1 2 64 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 171 2 0.0 3 0 0 0 2 234 1 0.0 3 0 0 0 1 247 1 0.0 3 0 0 0 1 253 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.11 s (33 us/read; 1.80 M reads/minute). === Summary === Total reads processed: 3,306 Reads with adapters: 143 (4.3%) Reads written (passing filters): 3,306 (100.0%) Total basepairs processed: 907,041 bp Total written (filtered): 906,609 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 143 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 46.9% C: 14.0% G: 37.1% T: 2.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 140 51.7 0 140 4 3 12.9 0 3