This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GATATT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 89.13 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 69,004 (2.0%) Reads that were too short: 23,866 (0.7%) Reads written (passing filters): 45,138 (1.3%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 9,486,173 bp (0.9%) === Adapter 1 === Sequence: GATATT; Type: regular 5'; Length: 6; Trimmed: 69004 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 104 846.0 0 104 7 3438 846.0 0 3438 8 140 846.0 0 140 9 6406 846.0 0 6406 10 78 846.0 0 78 11 74 846.0 0 74 12 45 846.0 0 45 13 63 846.0 0 63 14 79 846.0 0 79 15 60 846.0 0 60 16 64 846.0 0 64 17 79 846.0 0 79 18 72 846.0 0 72 19 65 846.0 0 65 20 65 846.0 0 65 21 80 846.0 0 80 22 65 846.0 0 65 23 64 846.0 0 64 24 79 846.0 0 79 25 86 846.0 0 86 26 90 846.0 0 90 27 67 846.0 0 67 28 61 846.0 0 61 29 88 846.0 0 88 30 66 846.0 0 66 31 66 846.0 0 66 32 65 846.0 0 65 33 80 846.0 0 80 34 107 846.0 0 107 35 451 846.0 0 451 36 264 846.0 0 264 37 471 846.0 0 471 38 66 846.0 0 66 39 61 846.0 0 61 40 64 846.0 0 64 41 74 846.0 0 74 42 585 846.0 0 585 43 93 846.0 0 93 44 72 846.0 0 72 45 66 846.0 0 66 46 66 846.0 0 66 47 43 846.0 0 43 48 50 846.0 0 50 49 60 846.0 0 60 50 58 846.0 0 58 51 60 846.0 0 60 52 75 846.0 0 75 53 73 846.0 0 73 54 56 846.0 0 56 55 56 846.0 0 56 56 68 846.0 0 68 57 58 846.0 0 58 58 51 846.0 0 51 59 77 846.0 0 77 60 83 846.0 0 83 61 50 846.0 0 50 62 55 846.0 0 55 63 67 846.0 0 67 64 304 846.0 0 304 65 69 846.0 0 69 66 71 846.0 0 71 67 67 846.0 0 67 68 104 846.0 0 104 69 956 846.0 0 956 70 298 846.0 0 298 71 316 846.0 0 316 72 48 846.0 0 48 73 63 846.0 0 63 74 54 846.0 0 54 75 69 846.0 0 69 76 70 846.0 0 70 77 57 846.0 0 57 78 61 846.0 0 61 79 53 846.0 0 53 80 58 846.0 0 58 81 84 846.0 0 84 82 79 846.0 0 79 83 85 846.0 0 85 84 75 846.0 0 75 85 84 846.0 0 84 86 85 846.0 0 85 87 70 846.0 0 70 88 144 846.0 0 144 89 275 846.0 0 275 90 356 846.0 0 356 91 83 846.0 0 83 92 87 846.0 0 87 93 95 846.0 0 95 94 217 846.0 0 217 95 159 846.0 0 159 96 288 846.0 0 288 97 474 846.0 0 474 98 623 846.0 0 623 99 1808 846.0 0 1808 100 2871 846.0 0 2871 101 469 846.0 0 469 102 164 846.0 0 164 103 101 846.0 0 101 104 81 846.0 0 81 105 268 846.0 0 268 106 155 846.0 0 155 107 187 846.0 0 187 108 120 846.0 0 120 109 112 846.0 0 112 110 348 846.0 0 348 111 559 846.0 0 559 112 421 846.0 0 421 113 171 846.0 0 171 114 153 846.0 0 153 115 94 846.0 0 94 116 103 846.0 0 103 117 84 846.0 0 84 118 121 846.0 0 121 119 133 846.0 0 133 120 119 846.0 0 119 121 306 846.0 0 306 122 360 846.0 0 360 123 282 846.0 0 282 124 143 846.0 0 143 125 153 846.0 0 153 126 153 846.0 0 153 127 187 846.0 0 187 128 156 846.0 0 156 129 173 846.0 0 173 130 138 846.0 0 138 131 228 846.0 0 228 132 163 846.0 0 163 133 182 846.0 0 182 134 198 846.0 0 198 135 141 846.0 0 141 136 129 846.0 0 129 137 113 846.0 0 113 138 130 846.0 0 130 139 273 846.0 0 273 140 293 846.0 0 293 141 589 846.0 0 589 142 164 846.0 0 164 143 287 846.0 0 287 144 138 846.0 0 138 145 139 846.0 0 139 146 273 846.0 0 273 147 242 846.0 0 242 148 204 846.0 0 204 149 143 846.0 0 143 150 82 846.0 0 82 151 99 846.0 0 99 152 119 846.0 0 119 153 133 846.0 0 133 154 144 846.0 0 144 155 168 846.0 0 168 156 116 846.0 0 116 157 157 846.0 0 157 158 148 846.0 0 148 159 375 846.0 0 375 160 232 846.0 0 232 161 325 846.0 0 325 162 114 846.0 0 114 163 136 846.0 0 136 164 153 846.0 0 153 165 141 846.0 0 141 166 123 846.0 0 123 167 152 846.0 0 152 168 237 846.0 0 237 169 160 846.0 0 160 170 158 846.0 0 158 171 140 846.0 0 140 172 165 846.0 0 165 173 205 846.0 0 205 174 157 846.0 0 157 175 154 846.0 0 154 176 139 846.0 0 139 177 141 846.0 0 141 178 133 846.0 0 133 179 166 846.0 0 166 180 165 846.0 0 165 181 135 846.0 0 135 182 179 846.0 0 179 183 172 846.0 0 172 184 148 846.0 0 148 185 192 846.0 0 192 186 158 846.0 0 158 187 136 846.0 0 136 188 144 846.0 0 144 189 134 846.0 0 134 190 157 846.0 0 157 191 149 846.0 0 149 192 163 846.0 0 163 193 207 846.0 0 207 194 311 846.0 0 311 195 308 846.0 0 308 196 181 846.0 0 181 197 363 846.0 0 363 198 97 846.0 0 97 199 136 846.0 0 136 200 319 846.0 0 319 201 240 846.0 0 240 202 405 846.0 0 405 203 231 846.0 0 231 204 179 846.0 0 179 205 181 846.0 0 181 206 560 846.0 0 560 207 888 846.0 0 888 208 686 846.0 0 686 209 441 846.0 0 441 210 264 846.0 0 264 211 222 846.0 0 222 212 163 846.0 0 163 213 194 846.0 0 194 214 191 846.0 0 191 215 159 846.0 0 159 216 160 846.0 0 160 217 111 846.0 0 111 218 163 846.0 0 163 219 131 846.0 0 131 220 222 846.0 0 222 221 272 846.0 0 272 222 267 846.0 0 267 223 236 846.0 0 236 224 170 846.0 0 170 225 163 846.0 0 163 226 165 846.0 0 165 227 146 846.0 0 146 228 167 846.0 0 167 229 241 846.0 0 241 230 231 846.0 0 231 231 218 846.0 0 218 232 243 846.0 0 243 233 202 846.0 0 202 234 229 846.0 0 229 235 273 846.0 0 273 236 378 846.0 0 378 237 496 846.0 0 496 238 265 846.0 0 265 239 354 846.0 0 354 240 372 846.0 0 372 241 640 846.0 0 640 242 768 846.0 0 768 243 311 846.0 0 311 244 179 846.0 0 179 245 150 846.0 0 150 246 88 846.0 0 88 247 99 846.0 0 99 248 264 846.0 0 264 249 430 846.0 0 430 250 608 846.0 0 608 251 155 846.0 0 155 252 146 846.0 0 146 253 128 846.0 0 128 254 159 846.0 0 159 255 192 846.0 0 192 256 232 846.0 0 232 257 178 846.0 0 178 258 207 846.0 0 207 259 360 846.0 0 360 260 484 846.0 0 484 261 409 846.0 0 409 262 327 846.0 0 327 263 154 846.0 0 154 264 160 846.0 0 160 265 145 846.0 0 145 266 113 846.0 0 113 267 93 846.0 0 93 268 118 846.0 0 118 269 155 846.0 0 155 270 211 846.0 0 211 271 155 846.0 0 155 272 150 846.0 0 150 273 205 846.0 0 205 274 272 846.0 0 272 275 274 846.0 0 274 276 141 846.0 0 141 277 107 846.0 0 107 278 149 846.0 0 149 279 139 846.0 0 139 280 176 846.0 0 176 281 231 846.0 0 231 282 220 846.0 0 220 283 298 846.0 0 298 284 168 846.0 0 168 285 109 846.0 0 109 286 103 846.0 0 103 287 96 846.0 0 96 288 158 846.0 0 158 289 254 846.0 0 254 290 176 846.0 0 176 291 146 846.0 0 146 292 105 846.0 0 105 293 127 846.0 0 127 294 137 846.0 0 137 295 248 846.0 0 248 296 229 846.0 0 229 297 191 846.0 0 191 298 241 846.0 0 241 299 155 846.0 0 155 300 234 846.0 0 234 301 170 846.0 0 170 Finished in 1.94 s (43 us/read; 1.40 M reads/minute). === Summary === Total reads processed: 45,138 Reads with adapters: 3,399 (7.5%) Reads that were too short: 14 (0.0%) Reads written (passing filters): 3,385 (7.5%) Total basepairs processed: 9,486,173 bp Total written (filtered): 924,415 bp (9.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 3399 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 1 0.0 3 0 0 1 19 36 0.0 3 0 35 0 0 1 20 3303 0.0 4 3259 38 5 0 1 21 15 0.0 4 6 8 0 1 22 1 0.0 4 1 23 1 0.0 4 0 0 0 1 41 1 0.0 4 0 0 0 0 1 43 1 0.0 4 0 0 0 0 1 50 1 0.0 4 0 0 0 0 1 60 1 0.0 4 0 0 0 0 1 80 1 0.0 4 0 0 0 0 1 88 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 25 0.0 4 0 0 0 0 25 141 1 0.0 4 0 0 0 0 1 144 1 0.0 4 0 0 0 0 1 145 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 154 1 0.0 4 0 0 0 0 1 159 1 0.0 4 0 0 0 0 1 197 1 0.0 4 0 0 0 0 1 198 1 0.0 4 0 0 0 0 1 231 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.11 s (32 us/read; 1.85 M reads/minute). === Summary === Total reads processed: 3,385 Reads with adapters: 23 (0.7%) Reads written (passing filters): 3,385 (100.0%) Total basepairs processed: 924,415 bp Total written (filtered): 923,012 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 23 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 26.1% C: 4.3% G: 65.2% T: 4.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 3 52.9 0 3 11 14 0.0 1 0 14 12 1 0.0 1 0 1 171 1 0.0 1 1 261 1 0.0 1 1 263 1 0.0 1 1 264 1 0.0 1 1 269 1 0.0 1 1