This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCGTTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 90.70 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 56,097 (1.6%) Reads that were too short: 15,673 (0.5%) Reads written (passing filters): 40,424 (1.2%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 9,592,714 bp (0.9%) === Adapter 1 === Sequence: TCGTTA; Type: regular 5'; Length: 6; Trimmed: 56097 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 280 846.0 0 280 7 328 846.0 0 328 8 20993 846.0 0 20993 9 76 846.0 0 76 10 29 846.0 0 29 11 24 846.0 0 24 12 34 846.0 0 34 13 24 846.0 0 24 14 31 846.0 0 31 15 18 846.0 0 18 16 39 846.0 0 39 17 23 846.0 0 23 18 40 846.0 0 40 19 44 846.0 0 44 20 28 846.0 0 28 21 39 846.0 0 39 22 44 846.0 0 44 23 42 846.0 0 42 24 24 846.0 0 24 25 38 846.0 0 38 26 25 846.0 0 25 27 29 846.0 0 29 28 16 846.0 0 16 29 35 846.0 0 35 30 42 846.0 0 42 31 43 846.0 0 43 32 35 846.0 0 35 33 52 846.0 0 52 34 39 846.0 0 39 35 29 846.0 0 29 36 32 846.0 0 32 37 40 846.0 0 40 38 38 846.0 0 38 39 29 846.0 0 29 40 36 846.0 0 36 41 42 846.0 0 42 42 51 846.0 0 51 43 29 846.0 0 29 44 34 846.0 0 34 45 30 846.0 0 30 46 33 846.0 0 33 47 32 846.0 0 32 48 21 846.0 0 21 49 32 846.0 0 32 50 28 846.0 0 28 51 38 846.0 0 38 52 37 846.0 0 37 53 39 846.0 0 39 54 41 846.0 0 41 55 33 846.0 0 33 56 36 846.0 0 36 57 68 846.0 0 68 58 45 846.0 0 45 59 26 846.0 0 26 60 62 846.0 0 62 61 37 846.0 0 37 62 36 846.0 0 36 63 45 846.0 0 45 64 32 846.0 0 32 65 35 846.0 0 35 66 47 846.0 0 47 67 67 846.0 0 67 68 44 846.0 0 44 69 30 846.0 0 30 70 40 846.0 0 40 71 39 846.0 0 39 72 33 846.0 0 33 73 38 846.0 0 38 74 149 846.0 0 149 75 63 846.0 0 63 76 69 846.0 0 69 77 40 846.0 0 40 78 115 846.0 0 115 79 68 846.0 0 68 80 50 846.0 0 50 81 43 846.0 0 43 82 59 846.0 0 59 83 65 846.0 0 65 84 71 846.0 0 71 85 96 846.0 0 96 86 50 846.0 0 50 87 119 846.0 0 119 88 105 846.0 0 105 89 67 846.0 0 67 90 46 846.0 0 46 91 110 846.0 0 110 92 66 846.0 0 66 93 115 846.0 0 115 94 182 846.0 0 182 95 286 846.0 0 286 96 131 846.0 0 131 97 381 846.0 0 381 98 1001 846.0 0 1001 99 353 846.0 0 353 100 295 846.0 0 295 101 74 846.0 0 74 102 57 846.0 0 57 103 45 846.0 0 45 104 38 846.0 0 38 105 42 846.0 0 42 106 38 846.0 0 38 107 52 846.0 0 52 108 54 846.0 0 54 109 101 846.0 0 101 110 71 846.0 0 71 111 112 846.0 0 112 112 410 846.0 0 410 113 285 846.0 0 285 114 88 846.0 0 88 115 93 846.0 0 93 116 76 846.0 0 76 117 69 846.0 0 69 118 30 846.0 0 30 119 76 846.0 0 76 120 83 846.0 0 83 121 76 846.0 0 76 122 61 846.0 0 61 123 48 846.0 0 48 124 64 846.0 0 64 125 86 846.0 0 86 126 83 846.0 0 83 127 77 846.0 0 77 128 95 846.0 0 95 129 205 846.0 0 205 130 105 846.0 0 105 131 149 846.0 0 149 132 776 846.0 0 776 133 161 846.0 0 161 134 254 846.0 0 254 135 117 846.0 0 117 136 116 846.0 0 116 137 70 846.0 0 70 138 88 846.0 0 88 139 64 846.0 0 64 140 83 846.0 0 83 141 86 846.0 0 86 142 76 846.0 0 76 143 45 846.0 0 45 144 78 846.0 0 78 145 68 846.0 0 68 146 154 846.0 0 154 147 123 846.0 0 123 148 112 846.0 0 112 149 87 846.0 0 87 150 75 846.0 0 75 151 64 846.0 0 64 152 66 846.0 0 66 153 50 846.0 0 50 154 184 846.0 0 184 155 147 846.0 0 147 156 147 846.0 0 147 157 95 846.0 0 95 158 63 846.0 0 63 159 92 846.0 0 92 160 75 846.0 0 75 161 74 846.0 0 74 162 59 846.0 0 59 163 45 846.0 0 45 164 73 846.0 0 73 165 81 846.0 0 81 166 168 846.0 0 168 167 117 846.0 0 117 168 92 846.0 0 92 169 80 846.0 0 80 170 54 846.0 0 54 171 52 846.0 0 52 172 61 846.0 0 61 173 70 846.0 0 70 174 167 846.0 0 167 175 186 846.0 0 186 176 66 846.0 0 66 177 84 846.0 0 84 178 71 846.0 0 71 179 154 846.0 0 154 180 151 846.0 0 151 181 179 846.0 0 179 182 93 846.0 0 93 183 85 846.0 0 85 184 96 846.0 0 96 185 134 846.0 0 134 186 137 846.0 0 137 187 91 846.0 0 91 188 79 846.0 0 79 189 66 846.0 0 66 190 74 846.0 0 74 191 83 846.0 0 83 192 102 846.0 0 102 193 229 846.0 0 229 194 529 846.0 0 529 195 461 846.0 0 461 196 379 846.0 0 379 197 108 846.0 0 108 198 159 846.0 0 159 199 256 846.0 0 256 200 234 846.0 0 234 201 193 846.0 0 193 202 179 846.0 0 179 203 63 846.0 0 63 204 100 846.0 0 100 205 125 846.0 0 125 206 126 846.0 0 126 207 147 846.0 0 147 208 172 846.0 0 172 209 113 846.0 0 113 210 126 846.0 0 126 211 88 846.0 0 88 212 106 846.0 0 106 213 83 846.0 0 83 214 102 846.0 0 102 215 156 846.0 0 156 216 140 846.0 0 140 217 141 846.0 0 141 218 72 846.0 0 72 219 126 846.0 0 126 220 638 846.0 0 638 221 330 846.0 0 330 222 364 846.0 0 364 223 184 846.0 0 184 224 91 846.0 0 91 225 75 846.0 0 75 226 98 846.0 0 98 227 96 846.0 0 96 228 119 846.0 0 119 229 100 846.0 0 100 230 64 846.0 0 64 231 103 846.0 0 103 232 71 846.0 0 71 233 85 846.0 0 85 234 91 846.0 0 91 235 126 846.0 0 126 236 115 846.0 0 115 237 251 846.0 0 251 238 308 846.0 0 308 239 181 846.0 0 181 240 96 846.0 0 96 241 138 846.0 0 138 242 118 846.0 0 118 243 325 846.0 0 325 244 398 846.0 0 398 245 173 846.0 0 173 246 146 846.0 0 146 247 114 846.0 0 114 248 108 846.0 0 108 249 65 846.0 0 65 250 48 846.0 0 48 251 67 846.0 0 67 252 158 846.0 0 158 253 176 846.0 0 176 254 453 846.0 0 453 255 372 846.0 0 372 256 1007 846.0 0 1007 257 199 846.0 0 199 258 209 846.0 0 209 259 96 846.0 0 96 260 68 846.0 0 68 261 63 846.0 0 63 262 525 846.0 0 525 263 321 846.0 0 321 264 1294 846.0 0 1294 265 98 846.0 0 98 266 99 846.0 0 99 267 121 846.0 0 121 268 316 846.0 0 316 269 212 846.0 0 212 270 257 846.0 0 257 271 66 846.0 0 66 272 59 846.0 0 59 273 86 846.0 0 86 274 94 846.0 0 94 275 55 846.0 0 55 276 64 846.0 0 64 277 47 846.0 0 47 278 43 846.0 0 43 279 103 846.0 0 103 280 133 846.0 0 133 281 142 846.0 0 142 282 161 846.0 0 161 283 59 846.0 0 59 284 70 846.0 0 70 285 68 846.0 0 68 286 83 846.0 0 83 287 69 846.0 0 69 288 60 846.0 0 60 289 67 846.0 0 67 290 38 846.0 0 38 291 75 846.0 0 75 292 57 846.0 0 57 293 80 846.0 0 80 294 97 846.0 0 97 295 81 846.0 0 81 296 34 846.0 0 34 297 38 846.0 0 38 298 30 846.0 0 30 299 20 846.0 0 20 300 36 846.0 0 36 301 63 846.0 0 63 Finished in 1.69 s (42 us/read; 1.44 M reads/minute). === Summary === Total reads processed: 40,424 Reads with adapters: 21,488 (53.2%) Reads that were too short: 18 (0.0%) Reads written (passing filters): 21,470 (53.1%) Total basepairs processed: 9,592,714 bp Total written (filtered): 5,922,718 bp (61.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 21488 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 6 0.0 3 0 0 4 2 16 251 0.0 3 0 234 12 5 17 21006 0.0 3 20461 486 46 13 18 122 0.0 3 46 57 9 10 19 19 0.0 3 2 1 11 5 20 42 0.0 3 0 0 1 41 21 1 0.0 3 0 0 0 1 25 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 40 1 0.0 3 1 42 1 0.0 3 0 1 43 1 0.0 3 0 0 0 1 59 6 0.0 3 0 0 0 6 62 3 0.0 3 0 0 0 3 63 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 76 5 0.0 3 0 0 0 5 77 3 0.0 3 0 0 0 3 96 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 175 2 0.0 3 0 0 1 1 182 1 0.0 3 0 0 0 1 187 2 0.0 3 0 0 0 2 188 3 0.0 3 0 0 0 3 189 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.75 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 21,470 Reads with adapters: 401 (1.9%) Reads written (passing filters): 21,470 (100.0%) Total basepairs processed: 5,922,718 bp Total written (filtered): 5,921,507 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 401 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 50.1% C: 18.5% G: 26.9% T: 4.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 395 335.5 0 395 4 4 83.9 0 4 5 2 21.0 0 2