This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCCTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 91.95 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 161,400 (4.7%) Reads that were too short: 12,235 (0.4%) Reads written (passing filters): 149,165 (4.3%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 26,265,118 bp (2.5%) === Adapter 1 === Sequence: AGCCTC; Type: regular 5'; Length: 6; Trimmed: 161400 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 60 846.0 0 60 7 2034 846.0 0 2034 8 79 846.0 0 79 9 67 846.0 0 67 10 73 846.0 0 73 11 2745 846.0 0 2745 12 78 846.0 0 78 13 54 846.0 0 54 14 258 846.0 0 258 15 53 846.0 0 53 16 57 846.0 0 57 17 50 846.0 0 50 18 82 846.0 0 82 19 52 846.0 0 52 20 54 846.0 0 54 21 47 846.0 0 47 22 71 846.0 0 71 23 56 846.0 0 56 24 60 846.0 0 60 25 61 846.0 0 61 26 54 846.0 0 54 27 58 846.0 0 58 28 61 846.0 0 61 29 94 846.0 0 94 30 39 846.0 0 39 31 39 846.0 0 39 32 62 846.0 0 62 33 47 846.0 0 47 34 63 846.0 0 63 35 47 846.0 0 47 36 60 846.0 0 60 37 81 846.0 0 81 38 85 846.0 0 85 39 161 846.0 0 161 40 64 846.0 0 64 41 73 846.0 0 73 42 57 846.0 0 57 43 64 846.0 0 64 44 110 846.0 0 110 45 85 846.0 0 85 46 68 846.0 0 68 47 66 846.0 0 66 48 71 846.0 0 71 49 111 846.0 0 111 50 134 846.0 0 134 51 203 846.0 0 203 52 313 846.0 0 313 53 365 846.0 0 365 54 554 846.0 0 554 55 820 846.0 0 820 56 29385 846.0 0 29385 57 12193 846.0 0 12193 58 15473 846.0 0 15473 59 144 846.0 0 144 60 63 846.0 0 63 61 51 846.0 0 51 62 261 846.0 0 261 63 87 846.0 0 87 64 76 846.0 0 76 65 46 846.0 0 46 66 38 846.0 0 38 67 48 846.0 0 48 68 48 846.0 0 48 69 52 846.0 0 52 70 71 846.0 0 71 71 44 846.0 0 44 72 72 846.0 0 72 73 75 846.0 0 75 74 85 846.0 0 85 75 51 846.0 0 51 76 42 846.0 0 42 77 43 846.0 0 43 78 56 846.0 0 56 79 47 846.0 0 47 80 117 846.0 0 117 81 91 846.0 0 91 82 89 846.0 0 89 83 201 846.0 0 201 84 250 846.0 0 250 85 97 846.0 0 97 86 105 846.0 0 105 87 343 846.0 0 343 88 283 846.0 0 283 89 655 846.0 0 655 90 130 846.0 0 130 91 50 846.0 0 50 92 69 846.0 0 69 93 94 846.0 0 94 94 73 846.0 0 73 95 130 846.0 0 130 96 56 846.0 0 56 97 44 846.0 0 44 98 49 846.0 0 49 99 51 846.0 0 51 100 54 846.0 0 54 101 59 846.0 0 59 102 57 846.0 0 57 103 240 846.0 0 240 104 67 846.0 0 67 105 93 846.0 0 93 106 46 846.0 0 46 107 41 846.0 0 41 108 50 846.0 0 50 109 55 846.0 0 55 110 55 846.0 0 55 111 108 846.0 0 108 112 52 846.0 0 52 113 49 846.0 0 49 114 54 846.0 0 54 115 43 846.0 0 43 116 59 846.0 0 59 117 53 846.0 0 53 118 62 846.0 0 62 119 71 846.0 0 71 120 92 846.0 0 92 121 71 846.0 0 71 122 58 846.0 0 58 123 83 846.0 0 83 124 313 846.0 0 313 125 312 846.0 0 312 126 822 846.0 0 822 127 94 846.0 0 94 128 114 846.0 0 114 129 67 846.0 0 67 130 70 846.0 0 70 131 53 846.0 0 53 132 76 846.0 0 76 133 72 846.0 0 72 134 66 846.0 0 66 135 67 846.0 0 67 136 75 846.0 0 75 137 72 846.0 0 72 138 92 846.0 0 92 139 90 846.0 0 90 140 70 846.0 0 70 141 87 846.0 0 87 142 68 846.0 0 68 143 89 846.0 0 89 144 87 846.0 0 87 145 105 846.0 0 105 146 300 846.0 0 300 147 724 846.0 0 724 148 376 846.0 0 376 149 379 846.0 0 379 150 1013 846.0 0 1013 151 252 846.0 0 252 152 279 846.0 0 279 153 116 846.0 0 116 154 68 846.0 0 68 155 76 846.0 0 76 156 146 846.0 0 146 157 81 846.0 0 81 158 121 846.0 0 121 159 79 846.0 0 79 160 236 846.0 0 236 161 159 846.0 0 159 162 145 846.0 0 145 163 107 846.0 0 107 164 105 846.0 0 105 165 119 846.0 0 119 166 135 846.0 0 135 167 765 846.0 0 765 168 627 846.0 0 627 169 772 846.0 0 772 170 321 846.0 0 321 171 182 846.0 0 182 172 186 846.0 0 186 173 312 846.0 0 312 174 188 846.0 0 188 175 386 846.0 0 386 176 389 846.0 0 389 177 391 846.0 0 391 178 180 846.0 0 180 179 97 846.0 0 97 180 162 846.0 0 162 181 157 846.0 0 157 182 113 846.0 0 113 183 149 846.0 0 149 184 101 846.0 0 101 185 162 846.0 0 162 186 135 846.0 0 135 187 92 846.0 0 92 188 103 846.0 0 103 189 136 846.0 0 136 190 125 846.0 0 125 191 158 846.0 0 158 192 197 846.0 0 197 193 201 846.0 0 201 194 226 846.0 0 226 195 942 846.0 0 942 196 497 846.0 0 497 197 597 846.0 0 597 198 670 846.0 0 670 199 737 846.0 0 737 200 4887 846.0 0 4887 201 52445 846.0 0 52445 202 2380 846.0 0 2380 203 135 846.0 0 135 204 68 846.0 0 68 205 69 846.0 0 69 206 70 846.0 0 70 207 82 846.0 0 82 208 55 846.0 0 55 209 48 846.0 0 48 210 43 846.0 0 43 211 65 846.0 0 65 212 71 846.0 0 71 213 86 846.0 0 86 214 92 846.0 0 92 215 62 846.0 0 62 216 82 846.0 0 82 217 78 846.0 0 78 218 64 846.0 0 64 219 129 846.0 0 129 220 149 846.0 0 149 221 109 846.0 0 109 222 57 846.0 0 57 223 69 846.0 0 69 224 92 846.0 0 92 225 84 846.0 0 84 226 75 846.0 0 75 227 78 846.0 0 78 228 90 846.0 0 90 229 356 846.0 0 356 230 205 846.0 0 205 231 160 846.0 0 160 232 280 846.0 0 280 233 596 846.0 0 596 234 195 846.0 0 195 235 54 846.0 0 54 236 50 846.0 0 50 237 50 846.0 0 50 238 50 846.0 0 50 239 51 846.0 0 51 240 90 846.0 0 90 241 74 846.0 0 74 242 74 846.0 0 74 243 77 846.0 0 77 244 74 846.0 0 74 245 61 846.0 0 61 246 66 846.0 0 66 247 47 846.0 0 47 248 44 846.0 0 44 249 36 846.0 0 36 250 65 846.0 0 65 251 98 846.0 0 98 252 76 846.0 0 76 253 110 846.0 0 110 254 77 846.0 0 77 255 94 846.0 0 94 256 102 846.0 0 102 257 70 846.0 0 70 258 36 846.0 0 36 259 38 846.0 0 38 260 30 846.0 0 30 261 49 846.0 0 49 262 84 846.0 0 84 263 85 846.0 0 85 264 95 846.0 0 95 265 104 846.0 0 104 266 94 846.0 0 94 267 170 846.0 0 170 268 117 846.0 0 117 269 56 846.0 0 56 270 71 846.0 0 71 271 69 846.0 0 69 272 65 846.0 0 65 273 288 846.0 0 288 274 84 846.0 0 84 275 69 846.0 0 69 276 104 846.0 0 104 277 81 846.0 0 81 278 173 846.0 0 173 279 496 846.0 0 496 280 174 846.0 0 174 281 80 846.0 0 80 282 72 846.0 0 72 283 105 846.0 0 105 284 67 846.0 0 67 285 45 846.0 0 45 286 26 846.0 0 26 287 62 846.0 0 62 288 48 846.0 0 48 289 54 846.0 0 54 290 62 846.0 0 62 291 120 846.0 0 120 292 209 846.0 0 209 293 321 846.0 0 321 294 146 846.0 0 146 295 46 846.0 0 46 296 38 846.0 0 38 297 41 846.0 0 41 298 25 846.0 0 25 299 31 846.0 0 31 300 85 846.0 0 85 301 56 846.0 0 56 Finished in 5.02 s (34 us/read; 1.78 M reads/minute). === Summary === Total reads processed: 149,165 Reads with adapters: 2,257 (1.5%) Reads that were too short: 157 (0.1%) Reads written (passing filters): 2,100 (1.4%) Total basepairs processed: 26,265,118 bp Total written (filtered): 567,270 bp (2.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 2257 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 10 0.0 3 0 9 0 1 17 1986 0.0 3 1934 45 4 3 18 4 0.0 3 0 4 19 3 0.0 3 0 0 2 1 20 3 0.0 3 0 0 0 3 22 3 0.0 3 2 0 1 32 1 0.0 3 0 0 0 1 45 3 0.0 3 0 0 0 3 47 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 53 20 0.0 3 0 0 0 20 56 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 65 2 0.0 3 0 0 0 2 68 7 0.0 3 0 0 1 6 69 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 80 7 0.0 3 0 0 0 7 81 4 0.0 3 0 0 0 4 83 3 0.0 3 0 0 1 2 84 1 0.0 3 0 0 0 1 86 4 0.0 3 0 0 0 4 87 1 0.0 3 0 0 0 1 88 22 0.0 3 0 0 1 21 89 20 0.0 3 0 0 0 20 92 2 0.0 3 0 0 0 2 95 6 0.0 3 0 0 0 6 97 1 0.0 3 0 0 0 1 101 2 0.0 3 0 0 0 2 106 1 0.0 3 0 0 0 1 108 3 0.0 3 0 0 0 3 111 5 0.0 3 0 0 0 5 116 3 0.0 3 0 0 0 3 117 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 136 3 0.0 3 0 0 0 3 141 1 0.0 3 0 0 0 1 142 2 0.0 3 0 0 0 2 143 3 0.0 3 0 0 0 3 145 3 0.0 3 0 0 0 3 148 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 160 12 0.0 3 0 0 0 12 162 1 0.0 3 0 0 0 1 163 2 0.0 3 0 0 0 2 165 16 0.0 3 0 0 0 16 166 2 0.0 3 0 0 0 2 167 1 0.0 3 0 0 0 1 168 3 0.0 3 0 0 0 3 169 1 0.0 3 0 0 0 1 179 3 0.0 3 0 0 1 2 180 6 0.0 3 0 0 0 6 182 1 0.0 3 0 0 0 1 183 5 0.0 3 0 0 0 5 186 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 209 1 0.0 3 0 0 0 1 219 1 0.0 3 0 0 0 1 221 15 0.0 3 0 0 0 15 222 1 0.0 3 0 0 0 1 224 1 0.0 3 0 0 0 1 228 2 0.0 3 0 0 0 2 231 5 0.0 3 0 0 0 5 232 6 0.0 3 0 0 0 6 233 3 0.0 3 0 0 0 3 238 2 0.0 3 0 0 0 2 254 1 0.0 3 0 0 0 1 272 3 0.0 3 0 0 0 3 274 1 0.0 3 0 0 0 1 276 1 0.0 3 0 0 0 1 282 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.08 s (38 us/read; 1.57 M reads/minute). === Summary === Total reads processed: 2,100 Reads with adapters: 114 (5.4%) Reads written (passing filters): 2,100 (100.0%) Total basepairs processed: 567,270 bp Total written (filtered): 566,928 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 114 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 34.2% C: 48.2% G: 12.3% T: 5.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 114 32.8 0 114