This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CATTAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 93.15 s (27 us/read; 2.23 M reads/minute). === Summary === Total reads processed: 3,465,239 Reads with adapters: 627,096 (18.1%) Reads that were too short: 21,704 (0.6%) Reads written (passing filters): 605,392 (17.5%) Total basepairs processed: 1,043,036,939 bp Total written (filtered): 169,167,380 bp (16.2%) === Adapter 1 === Sequence: CATTAG; Type: regular 5'; Length: 6; Trimmed: 627096 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 5079 846.0 0 5079 7 515448 846.0 0 515448 8 2446 846.0 0 2446 9 49 846.0 0 49 10 52 846.0 0 52 11 28 846.0 0 28 12 45 846.0 0 45 13 40 846.0 0 40 14 50 846.0 0 50 15 38 846.0 0 38 16 38 846.0 0 38 17 104 846.0 0 104 18 66 846.0 0 66 19 41 846.0 0 41 20 78 846.0 0 78 21 102 846.0 0 102 22 135 846.0 0 135 23 109 846.0 0 109 24 99 846.0 0 99 25 102 846.0 0 102 26 115 846.0 0 115 27 130 846.0 0 130 28 190 846.0 0 190 29 209 846.0 0 209 30 228 846.0 0 228 31 266 846.0 0 266 32 4485 846.0 0 4485 33 255 846.0 0 255 34 195 846.0 0 195 35 83 846.0 0 83 36 57 846.0 0 57 37 73 846.0 0 73 38 66 846.0 0 66 39 59 846.0 0 59 40 52 846.0 0 52 41 76 846.0 0 76 42 79 846.0 0 79 43 119 846.0 0 119 44 487 846.0 0 487 45 634 846.0 0 634 46 96 846.0 0 96 47 48 846.0 0 48 48 46 846.0 0 46 49 42 846.0 0 42 50 50 846.0 0 50 51 43 846.0 0 43 52 63 846.0 0 63 53 51 846.0 0 51 54 63 846.0 0 63 55 67 846.0 0 67 56 66 846.0 0 66 57 61 846.0 0 61 58 198 846.0 0 198 59 262 846.0 0 262 60 90 846.0 0 90 61 41 846.0 0 41 62 61 846.0 0 61 63 56 846.0 0 56 64 44 846.0 0 44 65 42 846.0 0 42 66 67 846.0 0 67 67 103 846.0 0 103 68 115 846.0 0 115 69 150 846.0 0 150 70 185 846.0 0 185 71 273 846.0 0 273 72 6914 846.0 0 6914 73 3639 846.0 0 3639 74 4752 846.0 0 4752 75 69 846.0 0 69 76 52 846.0 0 52 77 57 846.0 0 57 78 50 846.0 0 50 79 55 846.0 0 55 80 45 846.0 0 45 81 79 846.0 0 79 82 61 846.0 0 61 83 77 846.0 0 77 84 72 846.0 0 72 85 63 846.0 0 63 86 59 846.0 0 59 87 54 846.0 0 54 88 437 846.0 0 437 89 1182 846.0 0 1182 90 463 846.0 0 463 91 477 846.0 0 477 92 651 846.0 0 651 93 126 846.0 0 126 94 113 846.0 0 113 95 207 846.0 0 207 96 351 846.0 0 351 97 289 846.0 0 289 98 71 846.0 0 71 99 94 846.0 0 94 100 108 846.0 0 108 101 91 846.0 0 91 102 76 846.0 0 76 103 45 846.0 0 45 104 61 846.0 0 61 105 57 846.0 0 57 106 53 846.0 0 53 107 96 846.0 0 96 108 228 846.0 0 228 109 182 846.0 0 182 110 187 846.0 0 187 111 77 846.0 0 77 112 155 846.0 0 155 113 206 846.0 0 206 114 211 846.0 0 211 115 122 846.0 0 122 116 106 846.0 0 106 117 95 846.0 0 95 118 94 846.0 0 94 119 249 846.0 0 249 120 137 846.0 0 137 121 192 846.0 0 192 122 159 846.0 0 159 123 172 846.0 0 172 124 128 846.0 0 128 125 147 846.0 0 147 126 213 846.0 0 213 127 280 846.0 0 280 128 624 846.0 0 624 129 1177 846.0 0 1177 130 1016 846.0 0 1016 131 251 846.0 0 251 132 272 846.0 0 272 133 458 846.0 0 458 134 1164 846.0 0 1164 135 744 846.0 0 744 136 581 846.0 0 581 137 603 846.0 0 603 138 392 846.0 0 392 139 572 846.0 0 572 140 812 846.0 0 812 141 1070 846.0 0 1070 142 1039 846.0 0 1039 143 3971 846.0 0 3971 144 6534 846.0 0 6534 145 13964 846.0 0 13964 146 243 846.0 0 243 147 157 846.0 0 157 148 179 846.0 0 179 149 203 846.0 0 203 150 142 846.0 0 142 151 96 846.0 0 96 152 123 846.0 0 123 153 99 846.0 0 99 154 147 846.0 0 147 155 320 846.0 0 320 156 375 846.0 0 375 157 592 846.0 0 592 158 384 846.0 0 384 159 131 846.0 0 131 160 119 846.0 0 119 161 144 846.0 0 144 162 187 846.0 0 187 163 375 846.0 0 375 164 429 846.0 0 429 165 999 846.0 0 999 166 170 846.0 0 170 167 214 846.0 0 214 168 163 846.0 0 163 169 153 846.0 0 153 170 134 846.0 0 134 171 108 846.0 0 108 172 195 846.0 0 195 173 110 846.0 0 110 174 180 846.0 0 180 175 201 846.0 0 201 176 189 846.0 0 189 177 128 846.0 0 128 178 90 846.0 0 90 179 114 846.0 0 114 180 142 846.0 0 142 181 143 846.0 0 143 182 189 846.0 0 189 183 155 846.0 0 155 184 183 846.0 0 183 185 164 846.0 0 164 186 157 846.0 0 157 187 173 846.0 0 173 188 166 846.0 0 166 189 162 846.0 0 162 190 136 846.0 0 136 191 161 846.0 0 161 192 175 846.0 0 175 193 146 846.0 0 146 194 260 846.0 0 260 195 215 846.0 0 215 196 251 846.0 0 251 197 199 846.0 0 199 198 120 846.0 0 120 199 122 846.0 0 122 200 137 846.0 0 137 201 123 846.0 0 123 202 397 846.0 0 397 203 341 846.0 0 341 204 271 846.0 0 271 205 218 846.0 0 218 206 168 846.0 0 168 207 185 846.0 0 185 208 225 846.0 0 225 209 341 846.0 0 341 210 241 846.0 0 241 211 196 846.0 0 196 212 175 846.0 0 175 213 217 846.0 0 217 214 179 846.0 0 179 215 546 846.0 0 546 216 572 846.0 0 572 217 571 846.0 0 571 218 157 846.0 0 157 219 88 846.0 0 88 220 162 846.0 0 162 221 234 846.0 0 234 222 202 846.0 0 202 223 254 846.0 0 254 224 164 846.0 0 164 225 160 846.0 0 160 226 283 846.0 0 283 227 244 846.0 0 244 228 175 846.0 0 175 229 177 846.0 0 177 230 211 846.0 0 211 231 262 846.0 0 262 232 198 846.0 0 198 233 241 846.0 0 241 234 229 846.0 0 229 235 299 846.0 0 299 236 345 846.0 0 345 237 223 846.0 0 223 238 170 846.0 0 170 239 270 846.0 0 270 240 135 846.0 0 135 241 141 846.0 0 141 242 155 846.0 0 155 243 203 846.0 0 203 244 243 846.0 0 243 245 333 846.0 0 333 246 182 846.0 0 182 247 124 846.0 0 124 248 138 846.0 0 138 249 135 846.0 0 135 250 85 846.0 0 85 251 124 846.0 0 124 252 186 846.0 0 186 253 169 846.0 0 169 254 191 846.0 0 191 255 246 846.0 0 246 256 250 846.0 0 250 257 168 846.0 0 168 258 154 846.0 0 154 259 232 846.0 0 232 260 250 846.0 0 250 261 449 846.0 0 449 262 356 846.0 0 356 263 338 846.0 0 338 264 199 846.0 0 199 265 186 846.0 0 186 266 146 846.0 0 146 267 192 846.0 0 192 268 155 846.0 0 155 269 162 846.0 0 162 270 251 846.0 0 251 271 187 846.0 0 187 272 151 846.0 0 151 273 154 846.0 0 154 274 181 846.0 0 181 275 186 846.0 0 186 276 167 846.0 0 167 277 173 846.0 0 173 278 162 846.0 0 162 279 166 846.0 0 166 280 235 846.0 0 235 281 232 846.0 0 232 282 209 846.0 0 209 283 242 846.0 0 242 284 191 846.0 0 191 285 214 846.0 0 214 286 229 846.0 0 229 287 274 846.0 0 274 288 245 846.0 0 245 289 144 846.0 0 144 290 150 846.0 0 150 291 234 846.0 0 234 292 157 846.0 0 157 293 185 846.0 0 185 294 162 846.0 0 162 295 205 846.0 0 205 296 251 846.0 0 251 297 227 846.0 0 227 298 191 846.0 0 191 299 185 846.0 0 185 300 128 846.0 0 128 301 18 846.0 0 18 Finished in 29.97 s (50 us/read; 1.21 M reads/minute). === Summary === Total reads processed: 605,392 Reads with adapters: 2,055 (0.3%) Reads that were too short: 50 (0.0%) Reads written (passing filters): 2,005 (0.3%) Total basepairs processed: 169,167,380 bp Total written (filtered): 545,088 bp (0.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 2055 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 19 20 0.0 3 0 19 0 0 1 20 1947 0.0 4 1913 33 1 21 8 0.0 4 1 7 22 2 0.0 4 0 0 1 1 23 1 0.0 4 0 0 0 1 39 5 0.0 4 0 0 0 0 5 41 3 0.0 4 2 0 0 0 1 42 2 0.0 4 0 0 0 0 2 46 1 0.0 4 0 0 0 0 1 49 1 0.0 4 0 0 0 0 1 53 2 0.0 4 2 59 1 0.0 4 0 0 0 0 1 64 1 0.0 4 0 0 0 1 74 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 0 1 91 2 0.0 4 0 0 0 0 2 92 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 112 1 0.0 4 0 0 0 0 1 127 6 0.0 4 0 0 0 0 6 138 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 146 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 157 1 0.0 4 0 0 0 0 1 160 1 0.0 4 0 0 0 1 168 1 0.0 4 0 0 0 0 1 170 1 0.0 4 0 0 0 0 1 173 1 0.0 4 0 0 0 0 1 183 1 0.0 4 0 0 0 0 1 195 1 0.0 4 0 0 0 0 1 200 1 0.0 4 0 0 0 0 1 202 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 0 0 1 211 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 215 1 0.0 4 0 0 0 1 220 1 0.0 4 0 0 0 0 1 221 1 0.0 4 0 0 0 0 1 228 2 0.0 4 0 0 0 0 2 230 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 241 4 0.0 4 0 0 0 0 4 245 3 0.0 4 0 0 0 0 3 246 1 0.0 4 0 0 0 0 1 252 1 0.0 4 0 0 0 0 1 255 1 0.0 4 0 0 0 0 1 257 7 0.0 4 0 0 0 0 7 258 1 0.0 4 0 0 0 0 1 282 3 0.0 4 0 0 0 0 3 283 3 0.0 4 0 0 0 0 3 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.07 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 2,005 Reads with adapters: 719 (35.9%) Reads written (passing filters): 2,005 (100.0%) Total basepairs processed: 545,088 bp Total written (filtered): 357,484 bp (65.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 719 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 13.8% C: 14.0% G: 71.1% T: 1.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 10 3 0.0 1 1 2 11 22 0.0 1 0 22 236 1 0.0 1 1 259 2 0.0 1 2 262 2 0.0 1 2 263 1 0.0 1 1 266 1 0.0 1 1 269 6 0.0 1 6 270 649 0.0 1 636 13 271 24 0.0 1 24 272 7 0.0 1 6 1 273 1 0.0 1 1