This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCAAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 70.81 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 368,911 (13.6%) Reads that were too short: 20,001 (0.7%) Reads written (passing filters): 348,910 (12.9%) Total basepairs processed: 815,312,680 bp Total written (filtered): 95,117,531 bp (11.7%) === Adapter 1 === Sequence: GTCAAT; Type: regular 5'; Length: 6; Trimmed: 368911 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1681 661.3 0 1681 7 23335 661.3 0 23335 8 3267 661.3 0 3267 9 215050 661.3 0 215050 10 469 661.3 0 469 11 46 661.3 0 46 12 54 661.3 0 54 13 68 661.3 0 68 14 53 661.3 0 53 15 40 661.3 0 40 16 72 661.3 0 72 17 64 661.3 0 64 18 54 661.3 0 54 19 42 661.3 0 42 20 56 661.3 0 56 21 51 661.3 0 51 22 62 661.3 0 62 23 63 661.3 0 63 24 65 661.3 0 65 25 68 661.3 0 68 26 78 661.3 0 78 27 87 661.3 0 87 28 126 661.3 0 126 29 242 661.3 0 242 30 115 661.3 0 115 31 130 661.3 0 130 32 189 661.3 0 189 33 205 661.3 0 205 34 97 661.3 0 97 35 139 661.3 0 139 36 185 661.3 0 185 37 162 661.3 0 162 38 196 661.3 0 196 39 171 661.3 0 171 40 166 661.3 0 166 41 230 661.3 0 230 42 3974 661.3 0 3974 43 4452 661.3 0 4452 44 4971 661.3 0 4971 45 112 661.3 0 112 46 65 661.3 0 65 47 79 661.3 0 79 48 284 661.3 0 284 49 101 661.3 0 101 50 126 661.3 0 126 51 80 661.3 0 80 52 128 661.3 0 128 53 211 661.3 0 211 54 104 661.3 0 104 55 136 661.3 0 136 56 92 661.3 0 92 57 100 661.3 0 100 58 145 661.3 0 145 59 207 661.3 0 207 60 290 661.3 0 290 61 470 661.3 0 470 62 523 661.3 0 523 63 1052 661.3 0 1052 64 1797 661.3 0 1797 65 28851 661.3 0 28851 66 10752 661.3 0 10752 67 25325 661.3 0 25325 68 195 661.3 0 195 69 48 661.3 0 48 70 56 661.3 0 56 71 49 661.3 0 49 72 46 661.3 0 46 73 51 661.3 0 51 74 46 661.3 0 46 75 47 661.3 0 47 76 109 661.3 0 109 77 82 661.3 0 82 78 59 661.3 0 59 79 60 661.3 0 60 80 74 661.3 0 74 81 86 661.3 0 86 82 77 661.3 0 77 83 67 661.3 0 67 84 66 661.3 0 66 85 82 661.3 0 82 86 125 661.3 0 125 87 135 661.3 0 135 88 146 661.3 0 146 89 124 661.3 0 124 90 108 661.3 0 108 91 68 661.3 0 68 92 74 661.3 0 74 93 167 661.3 0 167 94 142 661.3 0 142 95 112 661.3 0 112 96 179 661.3 0 179 97 264 661.3 0 264 98 139 661.3 0 139 99 91 661.3 0 91 100 131 661.3 0 131 101 160 661.3 0 160 102 179 661.3 0 179 103 101 661.3 0 101 104 105 661.3 0 105 105 125 661.3 0 125 106 104 661.3 0 104 107 74 661.3 0 74 108 61 661.3 0 61 109 46 661.3 0 46 110 73 661.3 0 73 111 118 661.3 0 118 112 152 661.3 0 152 113 108 661.3 0 108 114 127 661.3 0 127 115 99 661.3 0 99 116 71 661.3 0 71 117 84 661.3 0 84 118 133 661.3 0 133 119 141 661.3 0 141 120 108 661.3 0 108 121 91 661.3 0 91 122 68 661.3 0 68 123 87 661.3 0 87 124 105 661.3 0 105 125 105 661.3 0 105 126 121 661.3 0 121 127 123 661.3 0 123 128 121 661.3 0 121 129 115 661.3 0 115 130 94 661.3 0 94 131 104 661.3 0 104 132 169 661.3 0 169 133 259 661.3 0 259 134 232 661.3 0 232 135 141 661.3 0 141 136 190 661.3 0 190 137 185 661.3 0 185 138 326 661.3 0 326 139 317 661.3 0 317 140 205 661.3 0 205 141 123 661.3 0 123 142 91 661.3 0 91 143 124 661.3 0 124 144 123 661.3 0 123 145 133 661.3 0 133 146 116 661.3 0 116 147 126 661.3 0 126 148 91 661.3 0 91 149 141 661.3 0 141 150 111 661.3 0 111 151 102 661.3 0 102 152 85 661.3 0 85 153 107 661.3 0 107 154 156 661.3 0 156 155 125 661.3 0 125 156 115 661.3 0 115 157 139 661.3 0 139 158 173 661.3 0 173 159 233 661.3 0 233 160 171 661.3 0 171 161 166 661.3 0 166 162 88 661.3 0 88 163 163 661.3 0 163 164 169 661.3 0 169 165 143 661.3 0 143 166 103 661.3 0 103 167 111 661.3 0 111 168 141 661.3 0 141 169 140 661.3 0 140 170 131 661.3 0 131 171 145 661.3 0 145 172 162 661.3 0 162 173 142 661.3 0 142 174 165 661.3 0 165 175 116 661.3 0 116 176 128 661.3 0 128 177 132 661.3 0 132 178 133 661.3 0 133 179 171 661.3 0 171 180 182 661.3 0 182 181 137 661.3 0 137 182 127 661.3 0 127 183 141 661.3 0 141 184 193 661.3 0 193 185 224 661.3 0 224 186 199 661.3 0 199 187 162 661.3 0 162 188 145 661.3 0 145 189 155 661.3 0 155 190 122 661.3 0 122 191 118 661.3 0 118 192 111 661.3 0 111 193 99 661.3 0 99 194 245 661.3 0 245 195 152 661.3 0 152 196 122 661.3 0 122 197 109 661.3 0 109 198 96 661.3 0 96 199 170 661.3 0 170 200 249 661.3 0 249 201 181 661.3 0 181 202 104 661.3 0 104 203 118 661.3 0 118 204 125 661.3 0 125 205 170 661.3 0 170 206 211 661.3 0 211 207 240 661.3 0 240 208 183 661.3 0 183 209 162 661.3 0 162 210 143 661.3 0 143 211 173 661.3 0 173 212 146 661.3 0 146 213 131 661.3 0 131 214 141 661.3 0 141 215 195 661.3 0 195 216 207 661.3 0 207 217 209 661.3 0 209 218 159 661.3 0 159 219 174 661.3 0 174 220 271 661.3 0 271 221 171 661.3 0 171 222 200 661.3 0 200 223 183 661.3 0 183 224 306 661.3 0 306 225 176 661.3 0 176 226 175 661.3 0 175 227 139 661.3 0 139 228 174 661.3 0 174 229 230 661.3 0 230 230 230 661.3 0 230 231 175 661.3 0 175 232 92 661.3 0 92 233 96 661.3 0 96 234 115 661.3 0 115 235 152 661.3 0 152 236 230 661.3 0 230 237 177 661.3 0 177 238 150 661.3 0 150 239 168 661.3 0 168 240 180 661.3 0 180 241 164 661.3 0 164 242 149 661.3 0 149 243 210 661.3 0 210 244 212 661.3 0 212 245 224 661.3 0 224 246 162 661.3 0 162 247 224 661.3 0 224 248 214 661.3 0 214 249 280 661.3 0 280 250 129 661.3 0 129 251 138 661.3 0 138 252 81 661.3 0 81 253 158 661.3 0 158 254 260 661.3 0 260 255 841 661.3 0 841 256 566 661.3 0 566 257 808 661.3 0 808 258 422 661.3 0 422 259 336 661.3 0 336 260 273 661.3 0 273 261 436 661.3 0 436 262 205 661.3 0 205 263 113 661.3 0 113 264 106 661.3 0 106 265 175 661.3 0 175 266 230 661.3 0 230 267 259 661.3 0 259 268 241 661.3 0 241 269 266 661.3 0 266 270 203 661.3 0 203 271 108 661.3 0 108 272 179 661.3 0 179 273 89 661.3 0 89 274 166 661.3 0 166 275 239 661.3 0 239 276 333 661.3 0 333 277 75 661.3 0 75 278 64 661.3 0 64 279 124 661.3 0 124 280 223 661.3 0 223 281 224 661.3 0 224 282 117 661.3 0 117 283 99 661.3 0 99 284 95 661.3 0 95 285 81 661.3 0 81 286 159 661.3 0 159 287 238 661.3 0 238 288 249 661.3 0 249 289 143 661.3 0 143 290 116 661.3 0 116 291 114 661.3 0 114 292 184 661.3 0 184 293 198 661.3 0 198 294 305 661.3 0 305 295 207 661.3 0 207 296 159 661.3 0 159 297 164 661.3 0 164 298 117 661.3 0 117 299 155 661.3 0 155 300 223 661.3 0 223 301 188 661.3 0 188 Finished in 14.15 s (41 us/read; 1.48 M reads/minute). === Summary === Total reads processed: 348,910 Reads with adapters: 220,670 (63.2%) Reads that were too short: 35 (0.0%) Reads written (passing filters): 220,635 (63.2%) Total basepairs processed: 95,117,531 bp Total written (filtered): 60,677,157 bp (63.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 220670 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 10 0.0 2 0 0 0 10 15 67 0.0 3 0 0 53 14 16 2076 0.0 3 0 1987 73 16 17 217033 0.0 3 212414 3997 478 144 18 612 0.0 3 16 451 51 94 19 77 0.0 3 2 10 45 20 20 710 0.0 3 303 7 1 399 21 1 0.0 3 0 1 26 1 0.0 3 0 0 0 1 28 1 0.0 3 1 29 1 0.0 3 1 31 4 0.0 3 4 35 1 0.0 3 1 38 1 0.0 3 1 41 1 0.0 3 1 51 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 61 2 0.0 3 0 0 0 2 74 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 125 4 0.0 3 0 0 0 4 134 1 0.0 3 0 0 0 1 142 2 0.0 3 0 0 0 2 143 7 0.0 3 0 0 0 7 144 5 0.0 3 0 0 0 5 149 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 163 2 0.0 3 0 0 0 2 167 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 183 2 0.0 3 0 0 0 2 184 2 0.0 3 0 0 0 2 186 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 1 205 1 0.0 3 0 0 0 1 216 3 0.0 3 0 0 0 3 223 1 0.0 3 0 0 0 1 224 3 0.0 3 0 0 0 3 226 1 0.0 3 0 0 0 1 227 1 0.0 3 0 0 0 1 230 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 1 258 2 0.0 3 0 0 0 2 270 1 0.0 3 0 0 0 1 275 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 282 2 0.0 3 0 0 0 2 284 3 0.0 3 0 0 0 3 285 1 0.0 3 0 0 0 1 286 1 0.0 3 0 0 0 1 287 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 289 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 7.82 s (35 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 220,635 Reads with adapters: 13,354 (6.1%) Reads written (passing filters): 220,635 (100.0%) Total basepairs processed: 60,677,157 bp Total written (filtered): 60,637,025 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 13354 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 22.5% C: 3.4% G: 70.5% T: 3.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 13285 3447.4 0 13285 4 68 861.9 0 68 5 1 215.5 0 1