This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCCGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 69.45 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 635,555 (23.5%) Reads that were too short: 356,027 (13.1%) Reads written (passing filters): 279,528 (10.3%) Total basepairs processed: 815,312,680 bp Total written (filtered): 73,522,856 bp (9.0%) === Adapter 1 === Sequence: GTCCGG; Type: regular 5'; Length: 6; Trimmed: 635555 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 15653 661.3 0 15653 7 210514 661.3 0 210514 8 320 661.3 0 320 9 92 661.3 0 92 10 46 661.3 0 46 11 30 661.3 0 30 12 27 661.3 0 27 13 45 661.3 0 45 14 20 661.3 0 20 15 19 661.3 0 19 16 27 661.3 0 27 17 41 661.3 0 41 18 34 661.3 0 34 19 35 661.3 0 35 20 35 661.3 0 35 21 48 661.3 0 48 22 29 661.3 0 29 23 20 661.3 0 20 24 29 661.3 0 29 25 35 661.3 0 35 26 28 661.3 0 28 27 25 661.3 0 25 28 31 661.3 0 31 29 31 661.3 0 31 30 25 661.3 0 25 31 59 661.3 0 59 32 728 661.3 0 728 33 63 661.3 0 63 34 52 661.3 0 52 35 44 661.3 0 44 36 37 661.3 0 37 37 24 661.3 0 24 38 33 661.3 0 33 39 32 661.3 0 32 40 16 661.3 0 16 41 32 661.3 0 32 42 34 661.3 0 34 43 39 661.3 0 39 44 41 661.3 0 41 45 39 661.3 0 39 46 18 661.3 0 18 47 22 661.3 0 22 48 33 661.3 0 33 49 18 661.3 0 18 50 20 661.3 0 20 51 24 661.3 0 24 52 30 661.3 0 30 53 35 661.3 0 35 54 28 661.3 0 28 55 59 661.3 0 59 56 34 661.3 0 34 57 51 661.3 0 51 58 33 661.3 0 33 59 37 661.3 0 37 60 36 661.3 0 36 61 55 661.3 0 55 62 28 661.3 0 28 63 20 661.3 0 20 64 17 661.3 0 17 65 45 661.3 0 45 66 32 661.3 0 32 67 35 661.3 0 35 68 36 661.3 0 36 69 31 661.3 0 31 70 28 661.3 0 28 71 33 661.3 0 33 72 41 661.3 0 41 73 211 661.3 0 211 74 31 661.3 0 31 75 189 661.3 0 189 76 100 661.3 0 100 77 123 661.3 0 123 78 18 661.3 0 18 79 38 661.3 0 38 80 29 661.3 0 29 81 31 661.3 0 31 82 21 661.3 0 21 83 25 661.3 0 25 84 24 661.3 0 24 85 26 661.3 0 26 86 12 661.3 0 12 87 24 661.3 0 24 88 23 661.3 0 23 89 21 661.3 0 21 90 27 661.3 0 27 91 30 661.3 0 30 92 26 661.3 0 26 93 37 661.3 0 37 94 31 661.3 0 31 95 34 661.3 0 34 96 40 661.3 0 40 97 37 661.3 0 37 98 33 661.3 0 33 99 47 661.3 0 47 100 92 661.3 0 92 101 25 661.3 0 25 102 52 661.3 0 52 103 56 661.3 0 56 104 41 661.3 0 41 105 32 661.3 0 32 106 38 661.3 0 38 107 40 661.3 0 40 108 26 661.3 0 26 109 25 661.3 0 25 110 48 661.3 0 48 111 46 661.3 0 46 112 67 661.3 0 67 113 368 661.3 0 368 114 541 661.3 0 541 115 2712 661.3 0 2712 116 38 661.3 0 38 117 20 661.3 0 20 118 21 661.3 0 21 119 25 661.3 0 25 120 18 661.3 0 18 121 107 661.3 0 107 122 22 661.3 0 22 123 30 661.3 0 30 124 28 661.3 0 28 125 23 661.3 0 23 126 31 661.3 0 31 127 156 661.3 0 156 128 29 661.3 0 29 129 47 661.3 0 47 130 35 661.3 0 35 131 38 661.3 0 38 132 38 661.3 0 38 133 63 661.3 0 63 134 77 661.3 0 77 135 54 661.3 0 54 136 58 661.3 0 58 137 74 661.3 0 74 138 103 661.3 0 103 139 52 661.3 0 52 140 78 661.3 0 78 141 61 661.3 0 61 142 261 661.3 0 261 143 200 661.3 0 200 144 557 661.3 0 557 145 65 661.3 0 65 146 92 661.3 0 92 147 801 661.3 0 801 148 3633 661.3 0 3633 149 1905 661.3 0 1905 150 161 661.3 0 161 151 16 661.3 0 16 152 16 661.3 0 16 153 25 661.3 0 25 154 16 661.3 0 16 155 26 661.3 0 26 156 25 661.3 0 25 157 25 661.3 0 25 158 33 661.3 0 33 159 17 661.3 0 17 160 25 661.3 0 25 161 34 661.3 0 34 162 29 661.3 0 29 163 21 661.3 0 21 164 19 661.3 0 19 165 28 661.3 0 28 166 18 661.3 0 18 167 27 661.3 0 27 168 37 661.3 0 37 169 33 661.3 0 33 170 28 661.3 0 28 171 23 661.3 0 23 172 25 661.3 0 25 173 32 661.3 0 32 174 22 661.3 0 22 175 21 661.3 0 21 176 37 661.3 0 37 177 32 661.3 0 32 178 37 661.3 0 37 179 57 661.3 0 57 180 47 661.3 0 47 181 90 661.3 0 90 182 77 661.3 0 77 183 85 661.3 0 85 184 63 661.3 0 63 185 103 661.3 0 103 186 170 661.3 0 170 187 315 661.3 0 315 188 284 661.3 0 284 189 861 661.3 0 861 190 639 661.3 0 639 191 987 661.3 0 987 192 1145 661.3 0 1145 193 611 661.3 0 611 194 641 661.3 0 641 195 975 661.3 0 975 196 761 661.3 0 761 197 701 661.3 0 701 198 854 661.3 0 854 199 1184 661.3 0 1184 200 6189 661.3 0 6189 201 18073 661.3 0 18073 202 7201 661.3 0 7201 203 1829 661.3 0 1829 204 1901 661.3 0 1901 205 14281 661.3 0 14281 206 32000 661.3 0 32000 207 57655 661.3 0 57655 208 14490 661.3 0 14490 209 6110 661.3 0 6110 210 1563 661.3 0 1563 211 2438 661.3 0 2438 212 2945 661.3 0 2945 213 2805 661.3 0 2805 214 771 661.3 0 771 215 943 661.3 0 943 216 1027 661.3 0 1027 217 754 661.3 0 754 218 1499 661.3 0 1499 219 3114 661.3 0 3114 220 3083 661.3 0 3083 221 3475 661.3 0 3475 222 3458 661.3 0 3458 223 2214 661.3 0 2214 224 2428 661.3 0 2428 225 6948 661.3 0 6948 226 36622 661.3 0 36622 227 72571 661.3 0 72571 228 39715 661.3 0 39715 229 239 661.3 0 239 230 83 661.3 0 83 231 181 661.3 0 181 232 25 661.3 0 25 233 14 661.3 0 14 234 25 661.3 0 25 235 21 661.3 0 21 236 22 661.3 0 22 237 20 661.3 0 20 238 30 661.3 0 30 239 18 661.3 0 18 240 26 661.3 0 26 241 20 661.3 0 20 242 23 661.3 0 23 243 25 661.3 0 25 244 74 661.3 0 74 245 88 661.3 0 88 246 104 661.3 0 104 247 79 661.3 0 79 248 131 661.3 0 131 249 290 661.3 0 290 250 66 661.3 0 66 251 218 661.3 0 218 252 250 661.3 0 250 253 146 661.3 0 146 254 103 661.3 0 103 255 246 661.3 0 246 256 169 661.3 0 169 257 316 661.3 0 316 258 160 661.3 0 160 259 284 661.3 0 284 260 233 661.3 0 233 261 367 661.3 0 367 262 3857 661.3 0 3857 263 7598 661.3 0 7598 264 15885 661.3 0 15885 265 78 661.3 0 78 266 36 661.3 0 36 267 18 661.3 0 18 268 17 661.3 0 17 269 15 661.3 0 15 270 19 661.3 0 19 271 9 661.3 0 9 272 48 661.3 0 48 273 10 661.3 0 10 274 65 661.3 0 65 275 42 661.3 0 42 276 11 661.3 0 11 277 6 661.3 0 6 278 9 661.3 0 9 279 13 661.3 0 13 280 14 661.3 0 14 281 9 661.3 0 9 282 11 661.3 0 11 283 12 661.3 0 12 284 10 661.3 0 10 285 16 661.3 0 16 286 9 661.3 0 9 287 16 661.3 0 16 288 12 661.3 0 12 289 18 661.3 0 18 290 22 661.3 0 22 291 9 661.3 0 9 292 22 661.3 0 22 293 23 661.3 0 23 294 38 661.3 0 38 295 13 661.3 0 13 296 18 661.3 0 18 297 20 661.3 0 20 298 19 661.3 0 19 299 15 661.3 0 15 300 10 661.3 0 10 301 19 661.3 0 19 Finished in 11.32 s (40 us/read; 1.48 M reads/minute). === Summary === Total reads processed: 279,528 Reads with adapters: 225,832 (80.8%) Reads that were too short: 4 (0.0%) Reads written (passing filters): 225,828 (80.8%) Total basepairs processed: 73,522,856 bp Total written (filtered): 61,893,336 bp (84.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 225832 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 4 0.0 3 0 0 0 3 1 18 117 0.0 3 0 0 114 3 19 3167 0.0 3 0 3080 77 6 4 20 221876 0.0 4 219012 2733 107 17 7 21 515 0.0 4 129 380 3 2 1 22 33 0.0 4 2 1 29 0 1 23 104 0.0 4 2 0 1 95 6 24 7 0.0 4 0 0 1 2 4 25 1 0.0 4 1 28 1 0.0 4 0 0 1 44 1 0.0 4 1 46 1 0.0 4 1 92 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 0 1 226 1 0.0 4 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 7.55 s (33 us/read; 1.79 M reads/minute). === Summary === Total reads processed: 225,828 Reads with adapters: 1,094 (0.5%) Reads written (passing filters): 225,828 (100.0%) Total basepairs processed: 61,893,336 bp Total written (filtered): 61,815,624 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1094 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 22.1% C: 6.9% G: 68.3% T: 2.6% none/other: 0.1% Overview of removed sequences length count expect max.err error counts 3 31 3528.6 0 31 4 9 882.1 0 9 5 5 220.5 0 5 10 6 0.2 1 0 6 11 687 0.1 1 6 681 12 67 0.0 1 3 64 13 2 0.0 1 0 2 15 1 0.0 1 0 1 25 12 0.0 1 9 3 26 1 0.0 1 1 91 2 0.0 1 2 152 12 0.0 1 0 12 153 2 0.0 1 0 2 158 1 0.0 1 1 171 1 0.0 1 1 193 2 0.0 1 0 2 194 1 0.0 1 0 1 210 9 0.0 1 1 8 259 1 0.0 1 1 260 11 0.0 1 11 261 108 0.0 1 105 3 262 75 0.0 1 71 4 263 4 0.0 1 3 1 264 34 0.0 1 32 2 265 6 0.0 1 5 1 271 4 0.0 1 4