This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTATGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 71.13 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 243,280 (9.0%) Reads that were too short: 21,742 (0.8%) Reads written (passing filters): 221,538 (8.2%) Total basepairs processed: 815,312,680 bp Total written (filtered): 61,850,757 bp (7.6%) === Adapter 1 === Sequence: GTATGT; Type: regular 5'; Length: 6; Trimmed: 243280 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1203 661.3 0 1203 7 1538 661.3 0 1538 8 25222 661.3 0 25222 9 166824 661.3 0 166824 10 259 661.3 0 259 11 74 661.3 0 74 12 67 661.3 0 67 13 64 661.3 0 64 14 48 661.3 0 48 15 49 661.3 0 49 16 56 661.3 0 56 17 55 661.3 0 55 18 54 661.3 0 54 19 58 661.3 0 58 20 61 661.3 0 61 21 46 661.3 0 46 22 150 661.3 0 150 23 49 661.3 0 49 24 84 661.3 0 84 25 61 661.3 0 61 26 67 661.3 0 67 27 103 661.3 0 103 28 70 661.3 0 70 29 66 661.3 0 66 30 71 661.3 0 71 31 246 661.3 0 246 32 31 661.3 0 31 33 49 661.3 0 49 34 69 661.3 0 69 35 72 661.3 0 72 36 69 661.3 0 69 37 66 661.3 0 66 38 57 661.3 0 57 39 43 661.3 0 43 40 92 661.3 0 92 41 41 661.3 0 41 42 60 661.3 0 60 43 64 661.3 0 64 44 57 661.3 0 57 45 58 661.3 0 58 46 51 661.3 0 51 47 47 661.3 0 47 48 43 661.3 0 43 49 52 661.3 0 52 50 41 661.3 0 41 51 49 661.3 0 49 52 47 661.3 0 47 53 60 661.3 0 60 54 88 661.3 0 88 55 52 661.3 0 52 56 34 661.3 0 34 57 47 661.3 0 47 58 49 661.3 0 49 59 35 661.3 0 35 60 47 661.3 0 47 61 46 661.3 0 46 62 36 661.3 0 36 63 65 661.3 0 65 64 45 661.3 0 45 65 47 661.3 0 47 66 61 661.3 0 61 67 74 661.3 0 74 68 79 661.3 0 79 69 53 661.3 0 53 70 63 661.3 0 63 71 116 661.3 0 116 72 56 661.3 0 56 73 60 661.3 0 60 74 55 661.3 0 55 75 66 661.3 0 66 76 182 661.3 0 182 77 116 661.3 0 116 78 127 661.3 0 127 79 117 661.3 0 117 80 755 661.3 0 755 81 369 661.3 0 369 82 459 661.3 0 459 83 163 661.3 0 163 84 73 661.3 0 73 85 85 661.3 0 85 86 142 661.3 0 142 87 91 661.3 0 91 88 67 661.3 0 67 89 122 661.3 0 122 90 116 661.3 0 116 91 116 661.3 0 116 92 98 661.3 0 98 93 145 661.3 0 145 94 122 661.3 0 122 95 130 661.3 0 130 96 1146 661.3 0 1146 97 1371 661.3 0 1371 98 2807 661.3 0 2807 99 141 661.3 0 141 100 89 661.3 0 89 101 62 661.3 0 62 102 69 661.3 0 69 103 58 661.3 0 58 104 54 661.3 0 54 105 55 661.3 0 55 106 57 661.3 0 57 107 66 661.3 0 66 108 73 661.3 0 73 109 114 661.3 0 114 110 72 661.3 0 72 111 86 661.3 0 86 112 82 661.3 0 82 113 88 661.3 0 88 114 117 661.3 0 117 115 91 661.3 0 91 116 63 661.3 0 63 117 65 661.3 0 65 118 80 661.3 0 80 119 61 661.3 0 61 120 62 661.3 0 62 121 71 661.3 0 71 122 65 661.3 0 65 123 74 661.3 0 74 124 76 661.3 0 76 125 64 661.3 0 64 126 75 661.3 0 75 127 94 661.3 0 94 128 176 661.3 0 176 129 156 661.3 0 156 130 202 661.3 0 202 131 87 661.3 0 87 132 380 661.3 0 380 133 152 661.3 0 152 134 272 661.3 0 272 135 104 661.3 0 104 136 103 661.3 0 103 137 111 661.3 0 111 138 108 661.3 0 108 139 89 661.3 0 89 140 96 661.3 0 96 141 82 661.3 0 82 142 93 661.3 0 93 143 78 661.3 0 78 144 87 661.3 0 87 145 82 661.3 0 82 146 76 661.3 0 76 147 99 661.3 0 99 148 232 661.3 0 232 149 751 661.3 0 751 150 304 661.3 0 304 151 78 661.3 0 78 152 76 661.3 0 76 153 89 661.3 0 89 154 217 661.3 0 217 155 90 661.3 0 90 156 101 661.3 0 101 157 90 661.3 0 90 158 80 661.3 0 80 159 86 661.3 0 86 160 101 661.3 0 101 161 120 661.3 0 120 162 106 661.3 0 106 163 102 661.3 0 102 164 117 661.3 0 117 165 97 661.3 0 97 166 83 661.3 0 83 167 109 661.3 0 109 168 79 661.3 0 79 169 114 661.3 0 114 170 71 661.3 0 71 171 77 661.3 0 77 172 83 661.3 0 83 173 88 661.3 0 88 174 103 661.3 0 103 175 89 661.3 0 89 176 109 661.3 0 109 177 95 661.3 0 95 178 109 661.3 0 109 179 105 661.3 0 105 180 129 661.3 0 129 181 112 661.3 0 112 182 117 661.3 0 117 183 114 661.3 0 114 184 131 661.3 0 131 185 194 661.3 0 194 186 140 661.3 0 140 187 347 661.3 0 347 188 516 661.3 0 516 189 938 661.3 0 938 190 557 661.3 0 557 191 149 661.3 0 149 192 140 661.3 0 140 193 88 661.3 0 88 194 88 661.3 0 88 195 97 661.3 0 97 196 190 661.3 0 190 197 101 661.3 0 101 198 88 661.3 0 88 199 46 661.3 0 46 200 75 661.3 0 75 201 117 661.3 0 117 202 120 661.3 0 120 203 96 661.3 0 96 204 114 661.3 0 114 205 129 661.3 0 129 206 117 661.3 0 117 207 156 661.3 0 156 208 153 661.3 0 153 209 367 661.3 0 367 210 492 661.3 0 492 211 587 661.3 0 587 212 239 661.3 0 239 213 135 661.3 0 135 214 143 661.3 0 143 215 122 661.3 0 122 216 106 661.3 0 106 217 118 661.3 0 118 218 110 661.3 0 110 219 109 661.3 0 109 220 113 661.3 0 113 221 143 661.3 0 143 222 164 661.3 0 164 223 142 661.3 0 142 224 148 661.3 0 148 225 160 661.3 0 160 226 187 661.3 0 187 227 154 661.3 0 154 228 263 661.3 0 263 229 103 661.3 0 103 230 118 661.3 0 118 231 166 661.3 0 166 232 212 661.3 0 212 233 155 661.3 0 155 234 223 661.3 0 223 235 227 661.3 0 227 236 169 661.3 0 169 237 181 661.3 0 181 238 236 661.3 0 236 239 125 661.3 0 125 240 475 661.3 0 475 241 1193 661.3 0 1193 242 1918 661.3 0 1918 243 272 661.3 0 272 244 245 661.3 0 245 245 160 661.3 0 160 246 117 661.3 0 117 247 126 661.3 0 126 248 125 661.3 0 125 249 562 661.3 0 562 250 773 661.3 0 773 251 1561 661.3 0 1561 252 155 661.3 0 155 253 399 661.3 0 399 254 672 661.3 0 672 255 373 661.3 0 373 256 181 661.3 0 181 257 120 661.3 0 120 258 78 661.3 0 78 259 127 661.3 0 127 260 316 661.3 0 316 261 441 661.3 0 441 262 472 661.3 0 472 263 124 661.3 0 124 264 96 661.3 0 96 265 110 661.3 0 110 266 102 661.3 0 102 267 84 661.3 0 84 268 125 661.3 0 125 269 180 661.3 0 180 270 256 661.3 0 256 271 376 661.3 0 376 272 183 661.3 0 183 273 177 661.3 0 177 274 211 661.3 0 211 275 89 661.3 0 89 276 113 661.3 0 113 277 86 661.3 0 86 278 65 661.3 0 65 279 68 661.3 0 68 280 55 661.3 0 55 281 95 661.3 0 95 282 92 661.3 0 92 283 104 661.3 0 104 284 97 661.3 0 97 285 113 661.3 0 113 286 69 661.3 0 69 287 36 661.3 0 36 288 62 661.3 0 62 289 76 661.3 0 76 290 78 661.3 0 78 291 79 661.3 0 79 292 66 661.3 0 66 293 48 661.3 0 48 294 90 661.3 0 90 295 73 661.3 0 73 296 83 661.3 0 83 297 77 661.3 0 77 298 69 661.3 0 69 299 63 661.3 0 63 300 41 661.3 0 41 301 68 661.3 0 68 Finished in 9.80 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 221,538 Reads with adapters: 22,518 (10.2%) Reads that were too short: 87 (0.0%) Reads written (passing filters): 22,431 (10.1%) Total basepairs processed: 61,850,757 bp Total written (filtered): 6,184,580 bp (10.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 22518 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 13 0.0 3 0 0 11 2 16 323 0.0 3 0 304 13 6 17 21835 0.0 3 21250 522 52 11 18 66 0.0 3 3 50 2 11 19 27 0.0 3 0 3 18 6 20 114 0.0 3 79 2 0 33 22 1 0.0 3 0 1 24 2 0.0 3 1 0 0 1 26 1 0.0 3 0 0 0 1 28 1 0.0 3 0 0 0 1 34 1 0.0 3 0 0 0 1 35 1 0.0 3 1 40 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 59 2 0.0 3 0 0 0 2 66 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 70 18 0.0 3 0 0 0 18 73 3 0.0 3 0 0 0 3 77 1 0.0 3 0 0 0 1 86 3 0.0 3 0 0 0 3 112 1 0.0 3 0 0 1 136 1 0.0 3 1 145 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 156 2 0.0 3 0 0 0 2 159 1 0.0 3 0 0 0 1 162 2 0.0 3 0 0 0 2 164 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 192 9 0.0 3 0 0 0 9 203 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 218 1 0.0 3 0 0 0 1 221 6 0.0 3 0 0 0 6 226 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 270 3 0.0 3 0 0 0 3 280 3 0.0 3 0 0 0 3 281 11 0.0 3 0 0 0 11 282 24 0.0 3 0 0 0 24 283 7 0.0 3 0 0 0 7 284 1 0.0 3 0 0 0 1 287 2 0.0 3 0 0 0 2 288 8 0.0 3 0 0 0 8 289 1 0.0 3 0 0 0 1 290 4 0.0 3 0 0 0 4 291 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.78 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 22,431 Reads with adapters: 249 (1.1%) Reads written (passing filters): 22,431 (100.0%) Total basepairs processed: 6,184,580 bp Total written (filtered): 6,183,821 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 249 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 44.6% C: 18.5% G: 19.3% T: 17.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 239 350.5 0 239 4 8 87.6 0 8 5 2 21.9 0 2