This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCCGCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 69.07 s (25 us/read; 2.35 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 64,276 (2.4%) Reads that were too short: 5,670 (0.2%) Reads written (passing filters): 58,606 (2.2%) Total basepairs processed: 815,312,680 bp Total written (filtered): 13,334,875 bp (1.6%) === Adapter 1 === Sequence: TCCGCT; Type: regular 5'; Length: 6; Trimmed: 64276 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 568 661.3 0 568 7 324 661.3 0 324 8 711 661.3 0 711 9 21827 661.3 0 21827 10 34 661.3 0 34 11 14 661.3 0 14 12 19 661.3 0 19 13 15 661.3 0 15 14 12 661.3 0 12 15 16 661.3 0 16 16 10 661.3 0 10 17 18 661.3 0 18 18 5 661.3 0 5 19 8 661.3 0 8 20 17 661.3 0 17 21 12 661.3 0 12 22 14 661.3 0 14 23 16 661.3 0 16 24 18 661.3 0 18 25 12 661.3 0 12 26 13 661.3 0 13 27 11 661.3 0 11 28 13 661.3 0 13 29 27 661.3 0 27 30 24 661.3 0 24 31 26 661.3 0 26 32 124 661.3 0 124 33 22 661.3 0 22 34 27 661.3 0 27 35 34 661.3 0 34 36 24 661.3 0 24 37 145 661.3 0 145 38 32 661.3 0 32 39 216 661.3 0 216 40 38 661.3 0 38 41 15 661.3 0 15 42 132 661.3 0 132 43 9 661.3 0 9 44 17 661.3 0 17 45 15 661.3 0 15 46 12 661.3 0 12 47 15 661.3 0 15 48 14 661.3 0 14 49 26 661.3 0 26 50 15 661.3 0 15 51 13 661.3 0 13 52 130 661.3 0 130 53 32 661.3 0 32 54 87 661.3 0 87 55 18 661.3 0 18 56 6 661.3 0 6 57 9 661.3 0 9 58 15 661.3 0 15 59 22 661.3 0 22 60 35 661.3 0 35 61 87 661.3 0 87 62 9287 661.3 0 9287 63 45 661.3 0 45 64 41 661.3 0 41 65 25 661.3 0 25 66 11 661.3 0 11 67 6 661.3 0 6 68 23 661.3 0 23 69 11 661.3 0 11 70 12 661.3 0 12 71 13 661.3 0 13 72 126 661.3 0 126 73 163 661.3 0 163 74 19 661.3 0 19 75 8 661.3 0 8 76 9 661.3 0 9 77 11 661.3 0 11 78 14 661.3 0 14 79 8 661.3 0 8 80 12 661.3 0 12 81 20 661.3 0 20 82 15 661.3 0 15 83 21 661.3 0 21 84 86 661.3 0 86 85 29 661.3 0 29 86 74 661.3 0 74 87 20 661.3 0 20 88 8 661.3 0 8 89 11 661.3 0 11 90 25 661.3 0 25 91 21 661.3 0 21 92 33 661.3 0 33 93 31 661.3 0 31 94 54 661.3 0 54 95 36 661.3 0 36 96 62 661.3 0 62 97 139 661.3 0 139 98 12 661.3 0 12 99 17 661.3 0 17 100 12 661.3 0 12 101 9 661.3 0 9 102 13 661.3 0 13 103 24 661.3 0 24 104 126 661.3 0 126 105 32 661.3 0 32 106 48 661.3 0 48 107 23 661.3 0 23 108 60 661.3 0 60 109 31 661.3 0 31 110 21 661.3 0 21 111 40 661.3 0 40 112 28 661.3 0 28 113 19 661.3 0 19 114 37 661.3 0 37 115 23 661.3 0 23 116 29 661.3 0 29 117 72 661.3 0 72 118 36 661.3 0 36 119 51 661.3 0 51 120 12 661.3 0 12 121 24 661.3 0 24 122 24 661.3 0 24 123 14 661.3 0 14 124 22 661.3 0 22 125 29 661.3 0 29 126 40 661.3 0 40 127 246 661.3 0 246 128 60 661.3 0 60 129 156 661.3 0 156 130 35 661.3 0 35 131 47 661.3 0 47 132 105 661.3 0 105 133 65 661.3 0 65 134 43 661.3 0 43 135 25 661.3 0 25 136 26 661.3 0 26 137 34 661.3 0 34 138 45 661.3 0 45 139 119 661.3 0 119 140 122 661.3 0 122 141 162 661.3 0 162 142 244 661.3 0 244 143 554 661.3 0 554 144 7649 661.3 0 7649 145 2746 661.3 0 2746 146 6816 661.3 0 6816 147 48 661.3 0 48 148 13 661.3 0 13 149 27 661.3 0 27 150 24 661.3 0 24 151 100 661.3 0 100 152 57 661.3 0 57 153 75 661.3 0 75 154 40 661.3 0 40 155 25 661.3 0 25 156 42 661.3 0 42 157 117 661.3 0 117 158 98 661.3 0 98 159 161 661.3 0 161 160 17 661.3 0 17 161 21 661.3 0 21 162 18 661.3 0 18 163 16 661.3 0 16 164 30 661.3 0 30 165 18 661.3 0 18 166 20 661.3 0 20 167 13 661.3 0 13 168 15 661.3 0 15 169 18 661.3 0 18 170 31 661.3 0 31 171 75 661.3 0 75 172 69 661.3 0 69 173 43 661.3 0 43 174 27 661.3 0 27 175 31 661.3 0 31 176 39 661.3 0 39 177 56 661.3 0 56 178 59 661.3 0 59 179 230 661.3 0 230 180 61 661.3 0 61 181 77 661.3 0 77 182 13 661.3 0 13 183 38 661.3 0 38 184 20 661.3 0 20 185 42 661.3 0 42 186 29 661.3 0 29 187 16 661.3 0 16 188 18 661.3 0 18 189 31 661.3 0 31 190 122 661.3 0 122 191 40 661.3 0 40 192 76 661.3 0 76 193 27 661.3 0 27 194 26 661.3 0 26 195 16 661.3 0 16 196 84 661.3 0 84 197 61 661.3 0 61 198 85 661.3 0 85 199 92 661.3 0 92 200 22 661.3 0 22 201 11 661.3 0 11 202 16 661.3 0 16 203 9 661.3 0 9 204 12 661.3 0 12 205 25 661.3 0 25 206 26 661.3 0 26 207 16 661.3 0 16 208 8 661.3 0 8 209 13 661.3 0 13 210 16 661.3 0 16 211 6 661.3 0 6 212 17 661.3 0 17 213 16 661.3 0 16 214 24 661.3 0 24 215 15 661.3 0 15 216 18 661.3 0 18 217 47 661.3 0 47 218 81 661.3 0 81 219 23 661.3 0 23 220 17 661.3 0 17 221 27 661.3 0 27 222 12 661.3 0 12 223 16 661.3 0 16 224 15 661.3 0 15 225 12 661.3 0 12 226 37 661.3 0 37 227 33 661.3 0 33 228 67 661.3 0 67 229 34 661.3 0 34 230 101 661.3 0 101 231 46 661.3 0 46 232 44 661.3 0 44 233 28 661.3 0 28 234 41 661.3 0 41 235 126 661.3 0 126 236 220 661.3 0 220 237 113 661.3 0 113 238 126 661.3 0 126 239 33 661.3 0 33 240 102 661.3 0 102 241 67 661.3 0 67 242 55 661.3 0 55 243 14 661.3 0 14 244 10 661.3 0 10 245 34 661.3 0 34 246 24 661.3 0 24 247 95 661.3 0 95 248 50 661.3 0 50 249 64 661.3 0 64 250 14 661.3 0 14 251 34 661.3 0 34 252 41 661.3 0 41 253 18 661.3 0 18 254 39 661.3 0 39 255 26 661.3 0 26 256 29 661.3 0 29 257 21 661.3 0 21 258 19 661.3 0 19 259 46 661.3 0 46 260 364 661.3 0 364 261 351 661.3 0 351 262 407 661.3 0 407 263 234 661.3 0 234 264 26 661.3 0 26 265 29 661.3 0 29 266 25 661.3 0 25 267 72 661.3 0 72 268 31 661.3 0 31 269 22 661.3 0 22 270 15 661.3 0 15 271 61 661.3 0 61 272 74 661.3 0 74 273 25 661.3 0 25 274 14 661.3 0 14 275 22 661.3 0 22 276 28 661.3 0 28 277 42 661.3 0 42 278 20 661.3 0 20 279 21 661.3 0 21 280 31 661.3 0 31 281 23 661.3 0 23 282 42 661.3 0 42 283 223 661.3 0 223 284 271 661.3 0 271 285 189 661.3 0 189 286 76 661.3 0 76 287 33 661.3 0 33 288 119 661.3 0 119 289 25 661.3 0 25 290 31 661.3 0 31 291 70 661.3 0 70 292 86 661.3 0 86 293 84 661.3 0 84 294 36 661.3 0 36 295 16 661.3 0 16 296 19 661.3 0 19 297 14 661.3 0 14 298 22 661.3 0 22 299 16 661.3 0 16 300 5 661.3 0 5 301 18 661.3 0 18 Finished in 2.57 s (44 us/read; 1.37 M reads/minute). === Summary === Total reads processed: 58,606 Reads with adapters: 23,346 (39.8%) Reads that were too short: 5 (0.0%) Reads written (passing filters): 23,341 (39.8%) Total basepairs processed: 13,334,875 bp Total written (filtered): 6,349,863 bp (47.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 23346 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 4 0.0 3 0 0 0 0 4 18 12 0.0 3 0 0 12 19 371 0.0 3 0 364 4 0 3 20 22534 0.0 4 22198 328 7 0 1 21 41 0.0 4 3 37 0 1 22 11 0.0 4 2 2 3 0 4 23 356 0.0 4 341 4 2 9 24 2 0.0 4 0 1 0 0 1 26 1 0.0 4 0 0 1 45 1 0.0 4 0 0 0 0 1 63 1 0.0 4 0 0 0 0 1 68 1 0.0 4 0 1 70 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 0 0 0 1 100 3 0.0 4 0 0 0 0 3 117 1 0.0 4 0 0 0 0 1 149 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 170 1 0.0 4 0 0 0 0 1 176 1 0.0 4 0 0 0 0 1 198 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.78 s (33 us/read; 1.80 M reads/minute). === Summary === Total reads processed: 23,341 Reads with adapters: 1,172 (5.0%) Reads written (passing filters): 23,341 (100.0%) Total basepairs processed: 6,349,863 bp Total written (filtered): 6,198,997 bp (97.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1172 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 24.1% C: 1.6% G: 70.2% T: 4.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 364.7 0 1 4 7 91.2 0 7 6 65 5.7 0 65 9 54 0.1 0 1 53 10 397 0.0 1 17 380 11 88 0.0 1 0 88 12 1 0.0 1 0 1 150 3 0.0 1 0 3 151 1 0.0 1 0 1 218 2 0.0 1 1 1 223 3 0.0 1 3 224 1 0.0 1 1 240 17 0.0 1 15 2 257 242 0.0 1 229 13 258 15 0.0 1 14 1 259 5 0.0 1 5 260 1 0.0 1 1 261 1 0.0 1 1 262 1 0.0 1 1 265 233 0.0 1 226 7 266 11 0.0 1 11 268 4 0.0 1 4 269 16 0.0 1 15 1 270 3 0.0 1 3