This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTTGCC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 69.30 s (26 us/read; 2.35 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 33,662 (1.2%) Reads that were too short: 4,399 (0.2%) Reads written (passing filters): 29,263 (1.1%) Total basepairs processed: 815,312,680 bp Total written (filtered): 7,615,348 bp (0.9%) === Adapter 1 === Sequence: GTTGCC; Type: regular 5'; Length: 6; Trimmed: 33662 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 270 661.3 0 270 7 7036 661.3 0 7036 8 11064 661.3 0 11064 9 86 661.3 0 86 10 33 661.3 0 33 11 49 661.3 0 49 12 35 661.3 0 35 13 45 661.3 0 45 14 46 661.3 0 46 15 30 661.3 0 30 16 31 661.3 0 31 17 39 661.3 0 39 18 19 661.3 0 19 19 35 661.3 0 35 20 28 661.3 0 28 21 26 661.3 0 26 22 34 661.3 0 34 23 24 661.3 0 24 24 30 661.3 0 30 25 29 661.3 0 29 26 32 661.3 0 32 27 36 661.3 0 36 28 23 661.3 0 23 29 31 661.3 0 31 30 26 661.3 0 26 31 27 661.3 0 27 32 42 661.3 0 42 33 33 661.3 0 33 34 30 661.3 0 30 35 40 661.3 0 40 36 45 661.3 0 45 37 32 661.3 0 32 38 49 661.3 0 49 39 41 661.3 0 41 40 42 661.3 0 42 41 31 661.3 0 31 42 31 661.3 0 31 43 32 661.3 0 32 44 31 661.3 0 31 45 29 661.3 0 29 46 33 661.3 0 33 47 43 661.3 0 43 48 1215 661.3 0 1215 49 88 661.3 0 88 50 19 661.3 0 19 51 29 661.3 0 29 52 20 661.3 0 20 53 27 661.3 0 27 54 42 661.3 0 42 55 45 661.3 0 45 56 30 661.3 0 30 57 26 661.3 0 26 58 23 661.3 0 23 59 31 661.3 0 31 60 68 661.3 0 68 61 29 661.3 0 29 62 27 661.3 0 27 63 32 661.3 0 32 64 29 661.3 0 29 65 37 661.3 0 37 66 36 661.3 0 36 67 35 661.3 0 35 68 44 661.3 0 44 69 257 661.3 0 257 70 104 661.3 0 104 71 318 661.3 0 318 72 584 661.3 0 584 73 236 661.3 0 236 74 205 661.3 0 205 75 33 661.3 0 33 76 18 661.3 0 18 77 13 661.3 0 13 78 25 661.3 0 25 79 56 661.3 0 56 80 45 661.3 0 45 81 88 661.3 0 88 82 58 661.3 0 58 83 88 661.3 0 88 84 428 661.3 0 428 85 114 661.3 0 114 86 162 661.3 0 162 87 22 661.3 0 22 88 31 661.3 0 31 89 30 661.3 0 30 90 35 661.3 0 35 91 58 661.3 0 58 92 20 661.3 0 20 93 26 661.3 0 26 94 39 661.3 0 39 95 41 661.3 0 41 96 30 661.3 0 30 97 36 661.3 0 36 98 24 661.3 0 24 99 31 661.3 0 31 100 32 661.3 0 32 101 34 661.3 0 34 102 42 661.3 0 42 103 33 661.3 0 33 104 16 661.3 0 16 105 19 661.3 0 19 106 24 661.3 0 24 107 30 661.3 0 30 108 30 661.3 0 30 109 30 661.3 0 30 110 74 661.3 0 74 111 258 661.3 0 258 112 30 661.3 0 30 113 19 661.3 0 19 114 39 661.3 0 39 115 37 661.3 0 37 116 25 661.3 0 25 117 26 661.3 0 26 118 22 661.3 0 22 119 37 661.3 0 37 120 29 661.3 0 29 121 35 661.3 0 35 122 29 661.3 0 29 123 37 661.3 0 37 124 25 661.3 0 25 125 83 661.3 0 83 126 27 661.3 0 27 127 18 661.3 0 18 128 20 661.3 0 20 129 26 661.3 0 26 130 28 661.3 0 28 131 156 661.3 0 156 132 41 661.3 0 41 133 29 661.3 0 29 134 29 661.3 0 29 135 18 661.3 0 18 136 30 661.3 0 30 137 35 661.3 0 35 138 40 661.3 0 40 139 25 661.3 0 25 140 29 661.3 0 29 141 19 661.3 0 19 142 47 661.3 0 47 143 30 661.3 0 30 144 20 661.3 0 20 145 27 661.3 0 27 146 23 661.3 0 23 147 55 661.3 0 55 148 86 661.3 0 86 149 53 661.3 0 53 150 70 661.3 0 70 151 31 661.3 0 31 152 32 661.3 0 32 153 34 661.3 0 34 154 30 661.3 0 30 155 24 661.3 0 24 156 50 661.3 0 50 157 51 661.3 0 51 158 47 661.3 0 47 159 53 661.3 0 53 160 29 661.3 0 29 161 42 661.3 0 42 162 30 661.3 0 30 163 27 661.3 0 27 164 27 661.3 0 27 165 33 661.3 0 33 166 25 661.3 0 25 167 31 661.3 0 31 168 79 661.3 0 79 169 54 661.3 0 54 170 57 661.3 0 57 171 37 661.3 0 37 172 28 661.3 0 28 173 25 661.3 0 25 174 21 661.3 0 21 175 32 661.3 0 32 176 134 661.3 0 134 177 52 661.3 0 52 178 83 661.3 0 83 179 21 661.3 0 21 180 22 661.3 0 22 181 23 661.3 0 23 182 69 661.3 0 69 183 43 661.3 0 43 184 48 661.3 0 48 185 33 661.3 0 33 186 31 661.3 0 31 187 34 661.3 0 34 188 50 661.3 0 50 189 40 661.3 0 40 190 42 661.3 0 42 191 24 661.3 0 24 192 28 661.3 0 28 193 29 661.3 0 29 194 29 661.3 0 29 195 58 661.3 0 58 196 48 661.3 0 48 197 64 661.3 0 64 198 114 661.3 0 114 199 163 661.3 0 163 200 25 661.3 0 25 201 16 661.3 0 16 202 48 661.3 0 48 203 106 661.3 0 106 204 155 661.3 0 155 205 103 661.3 0 103 206 76 661.3 0 76 207 31 661.3 0 31 208 22 661.3 0 22 209 39 661.3 0 39 210 61 661.3 0 61 211 41 661.3 0 41 212 43 661.3 0 43 213 91 661.3 0 91 214 131 661.3 0 131 215 142 661.3 0 142 216 36 661.3 0 36 217 47 661.3 0 47 218 35 661.3 0 35 219 37 661.3 0 37 220 26 661.3 0 26 221 39 661.3 0 39 222 59 661.3 0 59 223 165 661.3 0 165 224 387 661.3 0 387 225 292 661.3 0 292 226 25 661.3 0 25 227 26 661.3 0 26 228 38 661.3 0 38 229 22 661.3 0 22 230 16 661.3 0 16 231 23 661.3 0 23 232 20 661.3 0 20 233 29 661.3 0 29 234 30 661.3 0 30 235 33 661.3 0 33 236 31 661.3 0 31 237 24 661.3 0 24 238 25 661.3 0 25 239 27 661.3 0 27 240 37 661.3 0 37 241 24 661.3 0 24 242 34 661.3 0 34 243 43 661.3 0 43 244 49 661.3 0 49 245 41 661.3 0 41 246 40 661.3 0 40 247 30 661.3 0 30 248 20 661.3 0 20 249 15 661.3 0 15 250 16 661.3 0 16 251 26 661.3 0 26 252 18 661.3 0 18 253 25 661.3 0 25 254 40 661.3 0 40 255 46 661.3 0 46 256 31 661.3 0 31 257 30 661.3 0 30 258 65 661.3 0 65 259 64 661.3 0 64 260 54 661.3 0 54 261 28 661.3 0 28 262 23 661.3 0 23 263 36 661.3 0 36 264 38 661.3 0 38 265 19 661.3 0 19 266 21 661.3 0 21 267 20 661.3 0 20 268 13 661.3 0 13 269 18 661.3 0 18 270 21 661.3 0 21 271 47 661.3 0 47 272 24 661.3 0 24 273 30 661.3 0 30 274 19 661.3 0 19 275 28 661.3 0 28 276 28 661.3 0 28 277 27 661.3 0 27 278 38 661.3 0 38 279 18 661.3 0 18 280 28 661.3 0 28 281 21 661.3 0 21 282 36 661.3 0 36 283 46 661.3 0 46 284 43 661.3 0 43 285 30 661.3 0 30 286 27 661.3 0 27 287 37 661.3 0 37 288 18 661.3 0 18 289 20 661.3 0 20 290 16 661.3 0 16 291 22 661.3 0 22 292 17 661.3 0 17 293 15 661.3 0 15 294 16 661.3 0 16 295 12 661.3 0 12 296 20 661.3 0 20 297 28 661.3 0 28 298 36 661.3 0 36 299 36 661.3 0 36 300 25 661.3 0 25 301 25 661.3 0 25 Finished in 1.32 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 29,263 Reads with adapters: 6,786 (23.2%) Reads that were too short: 20 (0.1%) Reads written (passing filters): 6,766 (23.1%) Total basepairs processed: 7,615,348 bp Total written (filtered): 1,870,843 bp (24.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 6786 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 1 16 52 0.0 3 0 45 7 17 6659 0.0 3 6514 124 15 6 18 17 0.0 3 2 15 19 6 0.0 3 0 0 4 2 20 7 0.0 3 1 0 0 6 21 1 0.0 3 0 0 0 1 23 1 0.0 3 0 0 0 1 35 1 0.0 3 1 40 1 0.0 3 0 0 0 1 41 2 0.0 3 0 0 0 2 51 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 81 5 0.0 3 0 0 0 5 83 1 0.0 3 1 84 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 111 3 0.0 3 0 0 0 3 113 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 138 2 0.0 3 0 0 0 2 139 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 183 4 0.0 3 0 0 0 4 186 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 234 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 275 1 0.0 3 0 0 0 1 280 2 0.0 3 0 0 0 2 281 1 0.0 3 0 0 0 1 285 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.24 s (35 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 6,766 Reads with adapters: 531 (7.8%) Reads written (passing filters): 6,766 (100.0%) Total basepairs processed: 1,870,843 bp Total written (filtered): 1,869,247 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 531 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 20.9% C: 66.5% G: 10.0% T: 2.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 529 105.7 0 529 4 1 26.4 0 1 5 1 6.6 0 1