This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACAGGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 69.99 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 40,397 (1.5%) Reads that were too short: 10,035 (0.4%) Reads written (passing filters): 30,362 (1.1%) Total basepairs processed: 815,312,680 bp Total written (filtered): 6,543,714 bp (0.8%) === Adapter 1 === Sequence: ACAGGT; Type: regular 5'; Length: 6; Trimmed: 40397 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 143 661.3 0 143 7 215 661.3 0 215 8 6828 661.3 0 6828 9 29 661.3 0 29 10 35 661.3 0 35 11 30 661.3 0 30 12 24 661.3 0 24 13 46 661.3 0 46 14 24 661.3 0 24 15 31 661.3 0 31 16 26 661.3 0 26 17 66 661.3 0 66 18 55 661.3 0 55 19 30 661.3 0 30 20 45 661.3 0 45 21 42 661.3 0 42 22 50 661.3 0 50 23 25 661.3 0 25 24 30 661.3 0 30 25 25 661.3 0 25 26 27 661.3 0 27 27 35 661.3 0 35 28 30 661.3 0 30 29 34 661.3 0 34 30 24 661.3 0 24 31 31 661.3 0 31 32 39 661.3 0 39 33 37 661.3 0 37 34 41 661.3 0 41 35 54 661.3 0 54 36 43 661.3 0 43 37 26 661.3 0 26 38 34 661.3 0 34 39 43 661.3 0 43 40 32 661.3 0 32 41 34 661.3 0 34 42 46 661.3 0 46 43 23 661.3 0 23 44 46 661.3 0 46 45 30 661.3 0 30 46 30 661.3 0 30 47 46 661.3 0 46 48 43 661.3 0 43 49 25 661.3 0 25 50 24 661.3 0 24 51 49 661.3 0 49 52 28 661.3 0 28 53 35 661.3 0 35 54 54 661.3 0 54 55 42 661.3 0 42 56 30 661.3 0 30 57 42 661.3 0 42 58 56 661.3 0 56 59 50 661.3 0 50 60 35 661.3 0 35 61 45 661.3 0 45 62 35 661.3 0 35 63 32 661.3 0 32 64 36 661.3 0 36 65 48 661.3 0 48 66 62 661.3 0 62 67 70 661.3 0 70 68 87 661.3 0 87 69 169 661.3 0 169 70 244 661.3 0 244 71 3706 661.3 0 3706 72 1160 661.3 0 1160 73 2052 661.3 0 2052 74 51 661.3 0 51 75 19 661.3 0 19 76 25 661.3 0 25 77 39 661.3 0 39 78 35 661.3 0 35 79 56 661.3 0 56 80 35 661.3 0 35 81 34 661.3 0 34 82 25 661.3 0 25 83 34 661.3 0 34 84 39 661.3 0 39 85 44 661.3 0 44 86 41 661.3 0 41 87 42 661.3 0 42 88 26 661.3 0 26 89 36 661.3 0 36 90 63 661.3 0 63 91 63 661.3 0 63 92 51 661.3 0 51 93 422 661.3 0 422 94 134 661.3 0 134 95 147 661.3 0 147 96 677 661.3 0 677 97 331 661.3 0 331 98 1061 661.3 0 1061 99 124 661.3 0 124 100 56 661.3 0 56 101 46 661.3 0 46 102 37 661.3 0 37 103 42 661.3 0 42 104 37 661.3 0 37 105 29 661.3 0 29 106 49 661.3 0 49 107 42 661.3 0 42 108 66 661.3 0 66 109 77 661.3 0 77 110 43 661.3 0 43 111 44 661.3 0 44 112 40 661.3 0 40 113 49 661.3 0 49 114 76 661.3 0 76 115 92 661.3 0 92 116 58 661.3 0 58 117 53 661.3 0 53 118 74 661.3 0 74 119 57 661.3 0 57 120 48 661.3 0 48 121 64 661.3 0 64 122 49 661.3 0 49 123 76 661.3 0 76 124 393 661.3 0 393 125 79 661.3 0 79 126 149 661.3 0 149 127 81 661.3 0 81 128 76 661.3 0 76 129 72 661.3 0 72 130 122 661.3 0 122 131 80 661.3 0 80 132 91 661.3 0 91 133 76 661.3 0 76 134 75 661.3 0 75 135 100 661.3 0 100 136 95 661.3 0 95 137 92 661.3 0 92 138 80 661.3 0 80 139 60 661.3 0 60 140 94 661.3 0 94 141 71 661.3 0 71 142 58 661.3 0 58 143 101 661.3 0 101 144 108 661.3 0 108 145 93 661.3 0 93 146 63 661.3 0 63 147 50 661.3 0 50 148 47 661.3 0 47 149 53 661.3 0 53 150 78 661.3 0 78 151 67 661.3 0 67 152 59 661.3 0 59 153 85 661.3 0 85 154 106 661.3 0 106 155 114 661.3 0 114 156 101 661.3 0 101 157 72 661.3 0 72 158 61 661.3 0 61 159 64 661.3 0 64 160 66 661.3 0 66 161 70 661.3 0 70 162 47 661.3 0 47 163 71 661.3 0 71 164 94 661.3 0 94 165 105 661.3 0 105 166 112 661.3 0 112 167 77 661.3 0 77 168 81 661.3 0 81 169 68 661.3 0 68 170 72 661.3 0 72 171 73 661.3 0 73 172 114 661.3 0 114 173 154 661.3 0 154 174 135 661.3 0 135 175 161 661.3 0 161 176 62 661.3 0 62 177 69 661.3 0 69 178 78 661.3 0 78 179 95 661.3 0 95 180 92 661.3 0 92 181 94 661.3 0 94 182 86 661.3 0 86 183 94 661.3 0 94 184 95 661.3 0 95 185 96 661.3 0 96 186 65 661.3 0 65 187 67 661.3 0 67 188 70 661.3 0 70 189 75 661.3 0 75 190 158 661.3 0 158 191 277 661.3 0 277 192 664 661.3 0 664 193 438 661.3 0 438 194 540 661.3 0 540 195 121 661.3 0 121 196 80 661.3 0 80 197 92 661.3 0 92 198 85 661.3 0 85 199 60 661.3 0 60 200 66 661.3 0 66 201 76 661.3 0 76 202 69 661.3 0 69 203 151 661.3 0 151 204 241 661.3 0 241 205 134 661.3 0 134 206 86 661.3 0 86 207 151 661.3 0 151 208 66 661.3 0 66 209 73 661.3 0 73 210 137 661.3 0 137 211 138 661.3 0 138 212 109 661.3 0 109 213 111 661.3 0 111 214 78 661.3 0 78 215 92 661.3 0 92 216 101 661.3 0 101 217 121 661.3 0 121 218 84 661.3 0 84 219 70 661.3 0 70 220 72 661.3 0 72 221 91 661.3 0 91 222 82 661.3 0 82 223 75 661.3 0 75 224 74 661.3 0 74 225 123 661.3 0 123 226 123 661.3 0 123 227 92 661.3 0 92 228 72 661.3 0 72 229 73 661.3 0 73 230 59 661.3 0 59 231 75 661.3 0 75 232 75 661.3 0 75 233 78 661.3 0 78 234 93 661.3 0 93 235 110 661.3 0 110 236 109 661.3 0 109 237 115 661.3 0 115 238 92 661.3 0 92 239 58 661.3 0 58 240 76 661.3 0 76 241 83 661.3 0 83 242 90 661.3 0 90 243 73 661.3 0 73 244 61 661.3 0 61 245 99 661.3 0 99 246 53 661.3 0 53 247 138 661.3 0 138 248 35 661.3 0 35 249 47 661.3 0 47 250 68 661.3 0 68 251 105 661.3 0 105 252 76 661.3 0 76 253 164 661.3 0 164 254 118 661.3 0 118 255 109 661.3 0 109 256 95 661.3 0 95 257 91 661.3 0 91 258 86 661.3 0 86 259 87 661.3 0 87 260 116 661.3 0 116 261 97 661.3 0 97 262 116 661.3 0 116 263 91 661.3 0 91 264 104 661.3 0 104 265 150 661.3 0 150 266 89 661.3 0 89 267 58 661.3 0 58 268 46 661.3 0 46 269 81 661.3 0 81 270 125 661.3 0 125 271 190 661.3 0 190 272 334 661.3 0 334 273 137 661.3 0 137 274 90 661.3 0 90 275 78 661.3 0 78 276 126 661.3 0 126 277 110 661.3 0 110 278 146 661.3 0 146 279 69 661.3 0 69 280 83 661.3 0 83 281 66 661.3 0 66 282 87 661.3 0 87 283 70 661.3 0 70 284 106 661.3 0 106 285 95 661.3 0 95 286 98 661.3 0 98 287 49 661.3 0 49 288 60 661.3 0 60 289 129 661.3 0 129 290 165 661.3 0 165 291 197 661.3 0 197 292 104 661.3 0 104 293 73 661.3 0 73 294 41 661.3 0 41 295 70 661.3 0 70 296 66 661.3 0 66 297 130 661.3 0 130 298 128 661.3 0 128 299 121 661.3 0 121 300 166 661.3 0 166 301 71 661.3 0 71 Finished in 1.32 s (43 us/read; 1.38 M reads/minute). === Summary === Total reads processed: 30,362 Reads with adapters: 7,035 (23.2%) Reads that were too short: 2 (0.0%) Reads written (passing filters): 7,033 (23.2%) Total basepairs processed: 6,543,714 bp Total written (filtered): 1,920,340 bp (29.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 7035 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 46 0.0 3 0 0 0 1 45 18 2 0.0 3 0 0 2 19 156 0.0 3 0 154 2 20 6806 0.0 4 6732 71 2 0 1 21 13 0.0 4 4 9 22 4 0.0 4 0 0 3 0 1 23 4 0.0 4 0 0 0 3 1 24 1 0.0 4 1 47 1 0.0 4 0 0 0 0 1 51 1 0.0 4 0 0 0 0 1 119 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.24 s (34 us/read; 1.76 M reads/minute). === Summary === Total reads processed: 7,033 Reads with adapters: 508 (7.2%) Reads written (passing filters): 7,033 (100.0%) Total basepairs processed: 1,920,340 bp Total written (filtered): 1,852,812 bp (96.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 508 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 48.2% C: 0.8% G: 50.0% T: 1.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 109.9 0 1 10 18 0.0 1 1 17 11 240 0.0 1 2 238 259 179 0.0 1 176 3 260 14 0.0 1 13 1 261 2 0.0 1 2 262 48 0.0 1 43 5 263 2 0.0 1 2 268 2 0.0 1 2 271 1 0.0 1 1 273 1 0.0 1 1