This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATGGAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 69.58 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 129,253 (4.8%) Reads that were too short: 6,685 (0.2%) Reads written (passing filters): 122,568 (4.5%) Total basepairs processed: 815,312,680 bp Total written (filtered): 32,147,829 bp (3.9%) === Adapter 1 === Sequence: ATGGAT; Type: regular 5'; Length: 6; Trimmed: 129253 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1752 661.3 0 1752 7 78024 661.3 0 78024 8 129 661.3 0 129 9 48 661.3 0 48 10 33 661.3 0 33 11 52 661.3 0 52 12 36 661.3 0 36 13 44 661.3 0 44 14 34 661.3 0 34 15 45 661.3 0 45 16 76 661.3 0 76 17 137 661.3 0 137 18 100 661.3 0 100 19 32 661.3 0 32 20 28 661.3 0 28 21 39 661.3 0 39 22 48 661.3 0 48 23 38 661.3 0 38 24 34 661.3 0 34 25 42 661.3 0 42 26 34 661.3 0 34 27 29 661.3 0 29 28 45 661.3 0 45 29 50 661.3 0 50 30 50 661.3 0 50 31 44 661.3 0 44 32 103 661.3 0 103 33 869 661.3 0 869 34 38 661.3 0 38 35 37 661.3 0 37 36 36 661.3 0 36 37 41 661.3 0 41 38 49 661.3 0 49 39 44 661.3 0 44 40 26 661.3 0 26 41 42 661.3 0 42 42 44 661.3 0 44 43 43 661.3 0 43 44 48 661.3 0 48 45 46 661.3 0 46 46 228 661.3 0 228 47 85 661.3 0 85 48 171 661.3 0 171 49 51 661.3 0 51 50 40 661.3 0 40 51 54 661.3 0 54 52 44 661.3 0 44 53 35 661.3 0 35 54 36 661.3 0 36 55 28 661.3 0 28 56 51 661.3 0 51 57 45 661.3 0 45 58 44 661.3 0 44 59 40 661.3 0 40 60 43 661.3 0 43 61 37 661.3 0 37 62 24 661.3 0 24 63 60 661.3 0 60 64 38 661.3 0 38 65 50 661.3 0 50 66 38 661.3 0 38 67 46 661.3 0 46 68 46 661.3 0 46 69 33 661.3 0 33 70 41 661.3 0 41 71 32 661.3 0 32 72 43 661.3 0 43 73 41 661.3 0 41 74 48 661.3 0 48 75 37 661.3 0 37 76 59 661.3 0 59 77 55 661.3 0 55 78 64 661.3 0 64 79 118 661.3 0 118 80 277 661.3 0 277 81 417 661.3 0 417 82 108 661.3 0 108 83 148 661.3 0 148 84 232 661.3 0 232 85 148 661.3 0 148 86 106 661.3 0 106 87 154 661.3 0 154 88 125 661.3 0 125 89 170 661.3 0 170 90 159 661.3 0 159 91 173 661.3 0 173 92 236 661.3 0 236 93 373 661.3 0 373 94 5863 661.3 0 5863 95 6526 661.3 0 6526 96 12624 661.3 0 12624 97 754 661.3 0 754 98 1863 661.3 0 1863 99 66 661.3 0 66 100 74 661.3 0 74 101 59 661.3 0 59 102 45 661.3 0 45 103 37 661.3 0 37 104 45 661.3 0 45 105 34 661.3 0 34 106 42 661.3 0 42 107 45 661.3 0 45 108 68 661.3 0 68 109 84 661.3 0 84 110 283 661.3 0 283 111 215 661.3 0 215 112 232 661.3 0 232 113 110 661.3 0 110 114 53 661.3 0 53 115 72 661.3 0 72 116 63 661.3 0 63 117 64 661.3 0 64 118 62 661.3 0 62 119 55 661.3 0 55 120 58 661.3 0 58 121 48 661.3 0 48 122 56 661.3 0 56 123 43 661.3 0 43 124 141 661.3 0 141 125 82 661.3 0 82 126 323 661.3 0 323 127 83 661.3 0 83 128 71 661.3 0 71 129 64 661.3 0 64 130 89 661.3 0 89 131 174 661.3 0 174 132 146 661.3 0 146 133 99 661.3 0 99 134 56 661.3 0 56 135 51 661.3 0 51 136 62 661.3 0 62 137 219 661.3 0 219 138 189 661.3 0 189 139 256 661.3 0 256 140 72 661.3 0 72 141 87 661.3 0 87 142 67 661.3 0 67 143 88 661.3 0 88 144 85 661.3 0 85 145 88 661.3 0 88 146 101 661.3 0 101 147 61 661.3 0 61 148 47 661.3 0 47 149 54 661.3 0 54 150 34 661.3 0 34 151 36 661.3 0 36 152 30 661.3 0 30 153 25 661.3 0 25 154 36 661.3 0 36 155 47 661.3 0 47 156 51 661.3 0 51 157 50 661.3 0 50 158 160 661.3 0 160 159 58 661.3 0 58 160 63 661.3 0 63 161 57 661.3 0 57 162 37 661.3 0 37 163 31 661.3 0 31 164 40 661.3 0 40 165 42 661.3 0 42 166 49 661.3 0 49 167 52 661.3 0 52 168 39 661.3 0 39 169 43 661.3 0 43 170 43 661.3 0 43 171 37 661.3 0 37 172 40 661.3 0 40 173 42 661.3 0 42 174 36 661.3 0 36 175 53 661.3 0 53 176 53 661.3 0 53 177 44 661.3 0 44 178 32 661.3 0 32 179 32 661.3 0 32 180 66 661.3 0 66 181 54 661.3 0 54 182 47 661.3 0 47 183 49 661.3 0 49 184 55 661.3 0 55 185 69 661.3 0 69 186 60 661.3 0 60 187 80 661.3 0 80 188 39 661.3 0 39 189 29 661.3 0 29 190 39 661.3 0 39 191 47 661.3 0 47 192 120 661.3 0 120 193 112 661.3 0 112 194 294 661.3 0 294 195 50 661.3 0 50 196 32 661.3 0 32 197 34 661.3 0 34 198 54 661.3 0 54 199 62 661.3 0 62 200 48 661.3 0 48 201 18 661.3 0 18 202 28 661.3 0 28 203 51 661.3 0 51 204 97 661.3 0 97 205 382 661.3 0 382 206 205 661.3 0 205 207 172 661.3 0 172 208 43 661.3 0 43 209 61 661.3 0 61 210 37 661.3 0 37 211 65 661.3 0 65 212 47 661.3 0 47 213 67 661.3 0 67 214 54 661.3 0 54 215 34 661.3 0 34 216 49 661.3 0 49 217 45 661.3 0 45 218 70 661.3 0 70 219 62 661.3 0 62 220 57 661.3 0 57 221 35 661.3 0 35 222 42 661.3 0 42 223 59 661.3 0 59 224 57 661.3 0 57 225 35 661.3 0 35 226 41 661.3 0 41 227 73 661.3 0 73 228 65 661.3 0 65 229 64 661.3 0 64 230 53 661.3 0 53 231 34 661.3 0 34 232 52 661.3 0 52 233 48 661.3 0 48 234 43 661.3 0 43 235 47 661.3 0 47 236 81 661.3 0 81 237 74 661.3 0 74 238 75 661.3 0 75 239 86 661.3 0 86 240 63 661.3 0 63 241 47 661.3 0 47 242 37 661.3 0 37 243 51 661.3 0 51 244 41 661.3 0 41 245 54 661.3 0 54 246 31 661.3 0 31 247 60 661.3 0 60 248 33 661.3 0 33 249 37 661.3 0 37 250 26 661.3 0 26 251 14 661.3 0 14 252 51 661.3 0 51 253 70 661.3 0 70 254 44 661.3 0 44 255 45 661.3 0 45 256 44 661.3 0 44 257 58 661.3 0 58 258 61 661.3 0 61 259 45 661.3 0 45 260 35 661.3 0 35 261 72 661.3 0 72 262 74 661.3 0 74 263 85 661.3 0 85 264 45 661.3 0 45 265 55 661.3 0 55 266 44 661.3 0 44 267 76 661.3 0 76 268 48 661.3 0 48 269 26 661.3 0 26 270 21 661.3 0 21 271 170 661.3 0 170 272 488 661.3 0 488 273 112 661.3 0 112 274 44 661.3 0 44 275 41 661.3 0 41 276 41 661.3 0 41 277 37 661.3 0 37 278 49 661.3 0 49 279 54 661.3 0 54 280 58 661.3 0 58 281 49 661.3 0 49 282 34 661.3 0 34 283 57 661.3 0 57 284 59 661.3 0 59 285 50 661.3 0 50 286 115 661.3 0 115 287 86 661.3 0 86 288 110 661.3 0 110 289 53 661.3 0 53 290 46 661.3 0 46 291 91 661.3 0 91 292 90 661.3 0 90 293 70 661.3 0 70 294 45 661.3 0 45 295 46 661.3 0 46 296 58 661.3 0 58 297 50 661.3 0 50 298 82 661.3 0 82 299 107 661.3 0 107 300 87 661.3 0 87 301 23 661.3 0 23 Finished in 5.55 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 122,568 Reads with adapters: 79,748 (65.1%) Reads that were too short: 21 (0.0%) Reads written (passing filters): 79,727 (65.0%) Total basepairs processed: 32,147,829 bp Total written (filtered): 21,845,550 bp (68.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 79748 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 1 1 18 40 0.0 3 0 0 38 1 1 19 855 0.0 3 0 827 25 2 1 20 78526 0.0 4 77540 937 43 6 21 204 0.0 4 26 175 2 1 22 14 0.0 4 2 0 11 0 1 23 36 0.0 4 4 0 1 29 2 24 4 0.0 4 2 0 0 0 2 25 34 0.0 4 0 0 0 0 34 27 1 0.0 4 1 28 1 0.0 4 0 1 29 1 0.0 4 1 36 1 0.0 4 1 37 1 0.0 4 0 0 0 0 1 40 3 0.0 4 1 0 0 0 2 66 1 0.0 4 1 81 1 0.0 4 0 0 0 0 1 86 1 0.0 4 0 0 0 0 1 116 2 0.0 4 0 0 0 0 2 117 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 127 1 0.0 4 0 0 0 0 1 141 8 0.0 4 0 0 0 0 8 142 3 0.0 4 0 0 0 0 3 143 4 0.0 4 0 0 0 0 4 150 1 0.0 4 0 0 0 0 1 162 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.69 s (34 us/read; 1.78 M reads/minute). === Summary === Total reads processed: 79,727 Reads with adapters: 776 (1.0%) Reads written (passing filters): 79,727 (100.0%) Total basepairs processed: 21,845,550 bp Total written (filtered): 21,803,934 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 776 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 19.1% C: 1.5% G: 78.2% T: 1.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 16 1245.7 0 16 4 6 311.4 0 6 5 2 77.9 0 2 6 1 19.5 0 1 10 2 0.1 1 0 2 11 588 0.0 1 2 586 12 26 0.0 1 0 26 13 1 0.0 1 0 1 152 1 0.0 1 0 1 210 3 0.0 1 1 2 260 1 0.0 1 1 261 125 0.0 1 113 12 262 4 0.0 1 3 1