This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACACAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 69.93 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 623,874 (23.0%) Reads that were too short: 245,309 (9.1%) Reads written (passing filters): 378,565 (14.0%) Total basepairs processed: 815,312,680 bp Total written (filtered): 70,766,005 bp (8.7%) === Adapter 1 === Sequence: ACACAA; Type: regular 5'; Length: 6; Trimmed: 623874 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 987 661.3 0 987 7 15230 661.3 0 15230 8 1268 661.3 0 1268 9 50450 661.3 0 50450 10 354 661.3 0 354 11 31 661.3 0 31 12 33 661.3 0 33 13 21 661.3 0 21 14 37 661.3 0 37 15 18 661.3 0 18 16 19 661.3 0 19 17 39 661.3 0 39 18 24 661.3 0 24 19 21 661.3 0 21 20 29 661.3 0 29 21 26 661.3 0 26 22 26 661.3 0 26 23 25 661.3 0 25 24 49 661.3 0 49 25 25 661.3 0 25 26 30 661.3 0 30 27 29 661.3 0 29 28 54 661.3 0 54 29 38 661.3 0 38 30 42 661.3 0 42 31 55 661.3 0 55 32 67 661.3 0 67 33 49 661.3 0 49 34 37 661.3 0 37 35 24 661.3 0 24 36 38 661.3 0 38 37 38 661.3 0 38 38 21 661.3 0 21 39 50 661.3 0 50 40 129 661.3 0 129 41 58 661.3 0 58 42 122 661.3 0 122 43 175 661.3 0 175 44 55 661.3 0 55 45 47 661.3 0 47 46 39 661.3 0 39 47 38 661.3 0 38 48 43 661.3 0 43 49 52 661.3 0 52 50 168 661.3 0 168 51 1369 661.3 0 1369 52 1463 661.3 0 1463 53 27 661.3 0 27 54 33 661.3 0 33 55 40 661.3 0 40 56 26 661.3 0 26 57 50 661.3 0 50 58 34 661.3 0 34 59 37 661.3 0 37 60 29 661.3 0 29 61 36 661.3 0 36 62 54 661.3 0 54 63 59 661.3 0 59 64 53 661.3 0 53 65 58 661.3 0 58 66 137 661.3 0 137 67 58 661.3 0 58 68 175 661.3 0 175 69 180 661.3 0 180 70 93 661.3 0 93 71 396 661.3 0 396 72 2400 661.3 0 2400 73 3579 661.3 0 3579 74 2279 661.3 0 2279 75 2262 661.3 0 2262 76 1808 661.3 0 1808 77 1410 661.3 0 1410 78 1414 661.3 0 1414 79 1070 661.3 0 1070 80 1095 661.3 0 1095 81 766 661.3 0 766 82 1644 661.3 0 1644 83 1402 661.3 0 1402 84 1490 661.3 0 1490 85 1976 661.3 0 1976 86 1757 661.3 0 1757 87 1252 661.3 0 1252 88 1276 661.3 0 1276 89 1049 661.3 0 1049 90 1099 661.3 0 1099 91 2174 661.3 0 2174 92 1779 661.3 0 1779 93 1888 661.3 0 1888 94 1794 661.3 0 1794 95 1931 661.3 0 1931 96 1582 661.3 0 1582 97 1120 661.3 0 1120 98 628 661.3 0 628 99 865 661.3 0 865 100 786 661.3 0 786 101 1183 661.3 0 1183 102 1806 661.3 0 1806 103 3290 661.3 0 3290 104 4864 661.3 0 4864 105 5557 661.3 0 5557 106 4934 661.3 0 4934 107 3483 661.3 0 3483 108 2253 661.3 0 2253 109 2441 661.3 0 2441 110 1777 661.3 0 1777 111 2409 661.3 0 2409 112 2501 661.3 0 2501 113 2964 661.3 0 2964 114 2821 661.3 0 2821 115 2447 661.3 0 2447 116 2067 661.3 0 2067 117 1961 661.3 0 1961 118 2651 661.3 0 2651 119 2985 661.3 0 2985 120 2872 661.3 0 2872 121 2409 661.3 0 2409 122 1993 661.3 0 1993 123 2228 661.3 0 2228 124 2730 661.3 0 2730 125 3311 661.3 0 3311 126 3230 661.3 0 3230 127 2468 661.3 0 2468 128 2302 661.3 0 2302 129 2016 661.3 0 2016 130 2106 661.3 0 2106 131 1860 661.3 0 1860 132 2532 661.3 0 2532 133 4805 661.3 0 4805 134 4096 661.3 0 4096 135 3560 661.3 0 3560 136 2407 661.3 0 2407 137 1987 661.3 0 1987 138 1852 661.3 0 1852 139 1845 661.3 0 1845 140 2015 661.3 0 2015 141 2132 661.3 0 2132 142 2013 661.3 0 2013 143 2136 661.3 0 2136 144 2489 661.3 0 2489 145 2710 661.3 0 2710 146 2801 661.3 0 2801 147 2440 661.3 0 2440 148 3140 661.3 0 3140 149 2744 661.3 0 2744 150 2371 661.3 0 2371 151 2409 661.3 0 2409 152 2204 661.3 0 2204 153 2877 661.3 0 2877 154 2666 661.3 0 2666 155 2338 661.3 0 2338 156 2414 661.3 0 2414 157 2279 661.3 0 2279 158 2471 661.3 0 2471 159 2674 661.3 0 2674 160 2500 661.3 0 2500 161 2936 661.3 0 2936 162 4004 661.3 0 4004 163 3687 661.3 0 3687 164 3637 661.3 0 3637 165 2898 661.3 0 2898 166 2389 661.3 0 2389 167 2204 661.3 0 2204 168 2047 661.3 0 2047 169 2240 661.3 0 2240 170 2517 661.3 0 2517 171 2766 661.3 0 2766 172 2824 661.3 0 2824 173 2993 661.3 0 2993 174 2480 661.3 0 2480 175 1782 661.3 0 1782 176 1944 661.3 0 1944 177 1552 661.3 0 1552 178 2367 661.3 0 2367 179 2573 661.3 0 2573 180 2413 661.3 0 2413 181 2403 661.3 0 2403 182 2144 661.3 0 2144 183 2464 661.3 0 2464 184 2246 661.3 0 2246 185 2335 661.3 0 2335 186 2626 661.3 0 2626 187 2265 661.3 0 2265 188 2179 661.3 0 2179 189 2215 661.3 0 2215 190 2391 661.3 0 2391 191 2855 661.3 0 2855 192 2453 661.3 0 2453 193 2901 661.3 0 2901 194 2030 661.3 0 2030 195 1157 661.3 0 1157 196 1401 661.3 0 1401 197 1478 661.3 0 1478 198 2896 661.3 0 2896 199 2989 661.3 0 2989 200 1976 661.3 0 1976 201 1890 661.3 0 1890 202 1631 661.3 0 1631 203 1857 661.3 0 1857 204 1612 661.3 0 1612 205 1573 661.3 0 1573 206 1723 661.3 0 1723 207 1829 661.3 0 1829 208 1754 661.3 0 1754 209 1583 661.3 0 1583 210 1490 661.3 0 1490 211 1672 661.3 0 1672 212 2323 661.3 0 2323 213 12099 661.3 0 12099 214 3680 661.3 0 3680 215 9031 661.3 0 9031 216 2204 661.3 0 2204 217 2061 661.3 0 2061 218 2040 661.3 0 2040 219 2406 661.3 0 2406 220 2020 661.3 0 2020 221 1819 661.3 0 1819 222 2111 661.3 0 2111 223 1775 661.3 0 1775 224 2405 661.3 0 2405 225 2686 661.3 0 2686 226 2452 661.3 0 2452 227 2885 661.3 0 2885 228 2401 661.3 0 2401 229 2646 661.3 0 2646 230 2465 661.3 0 2465 231 1994 661.3 0 1994 232 1613 661.3 0 1613 233 1538 661.3 0 1538 234 1939 661.3 0 1939 235 2375 661.3 0 2375 236 2472 661.3 0 2472 237 1977 661.3 0 1977 238 2565 661.3 0 2565 239 1989 661.3 0 1989 240 1980 661.3 0 1980 241 1574 661.3 0 1574 242 1763 661.3 0 1763 243 1591 661.3 0 1591 244 1430 661.3 0 1430 245 1658 661.3 0 1658 246 2090 661.3 0 2090 247 3321 661.3 0 3321 248 3673 661.3 0 3673 249 2912 661.3 0 2912 250 3129 661.3 0 3129 251 2895 661.3 0 2895 252 2178 661.3 0 2178 253 2264 661.3 0 2264 254 1931 661.3 0 1931 255 1926 661.3 0 1926 256 2158 661.3 0 2158 257 2682 661.3 0 2682 258 2708 661.3 0 2708 259 2387 661.3 0 2387 260 1685 661.3 0 1685 261 1620 661.3 0 1620 262 1572 661.3 0 1572 263 1751 661.3 0 1751 264 2246 661.3 0 2246 265 2154 661.3 0 2154 266 2540 661.3 0 2540 267 2960 661.3 0 2960 268 2789 661.3 0 2789 269 2350 661.3 0 2350 270 1803 661.3 0 1803 271 2211 661.3 0 2211 272 1735 661.3 0 1735 273 2837 661.3 0 2837 274 2874 661.3 0 2874 275 2351 661.3 0 2351 276 1812 661.3 0 1812 277 1600 661.3 0 1600 278 2029 661.3 0 2029 279 2844 661.3 0 2844 280 2768 661.3 0 2768 281 2008 661.3 0 2008 282 2031 661.3 0 2031 283 1870 661.3 0 1870 284 2205 661.3 0 2205 285 2858 661.3 0 2858 286 3035 661.3 0 3035 287 2693 661.3 0 2693 288 2318 661.3 0 2318 289 2032 661.3 0 2032 290 3246 661.3 0 3246 291 4414 661.3 0 4414 292 2309 661.3 0 2309 293 2438 661.3 0 2438 294 2270 661.3 0 2270 295 2704 661.3 0 2704 296 2346 661.3 0 2346 297 2043 661.3 0 2043 298 1770 661.3 0 1770 299 2018 661.3 0 2018 300 2728 661.3 0 2728 301 6497 661.3 0 6497 Finished in 12.48 s (33 us/read; 1.82 M reads/minute). === Summary === Total reads processed: 378,565 Reads with adapters: 52,754 (13.9%) Reads that were too short: 130 (0.0%) Reads written (passing filters): 52,624 (13.9%) Total basepairs processed: 70,766,005 bp Total written (filtered): 14,458,071 bp (20.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 52754 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 6 0.0 2 0 0 0 6 15 19 0.0 3 0 0 15 4 16 428 0.0 3 0 402 19 7 17 51798 0.0 3 50525 1096 134 43 18 156 0.0 3 14 127 12 3 19 53 0.0 3 7 1 26 19 20 59 0.0 3 1 0 0 58 21 1 0.0 3 0 0 0 1 22 1 0.0 3 0 0 0 1 23 1 0.0 3 0 0 1 24 2 0.0 3 0 0 0 2 26 3 0.0 3 0 0 0 3 27 1 0.0 3 0 0 0 1 28 3 0.0 3 0 0 0 3 29 4 0.0 3 0 0 0 4 31 6 0.0 3 2 0 0 4 32 1 0.0 3 0 0 0 1 33 1 0.0 3 1 35 3 0.0 3 0 0 0 3 36 2 0.0 3 1 0 0 1 37 4 0.0 3 0 0 0 4 38 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 44 4 0.0 3 0 0 0 4 45 2 0.0 3 0 0 0 2 46 4 0.0 3 0 0 0 4 49 1 0.0 3 0 0 0 1 50 2 0.0 3 0 0 0 2 51 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 55 2 0.0 3 0 0 0 2 56 3 0.0 3 0 0 0 3 57 2 0.0 3 0 0 0 2 60 2 0.0 3 0 0 0 2 63 2 0.0 3 0 0 0 2 64 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 66 3 0.0 3 0 0 0 3 67 3 0.0 3 0 0 0 3 68 2 0.0 3 0 0 0 2 69 1 0.0 3 0 0 0 1 70 2 0.0 3 0 0 0 2 71 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 1 74 2 0.0 3 0 0 0 2 75 2 0.0 3 0 0 0 2 76 3 0.0 3 0 0 0 3 77 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 86 2 0.0 3 0 0 1 1 87 2 0.0 3 0 0 0 2 88 1 0.0 3 0 0 0 1 89 2 0.0 3 0 0 0 2 90 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 96 2 0.0 3 0 0 0 2 98 1 0.0 3 0 0 0 1 99 3 0.0 3 0 0 0 3 100 3 0.0 3 0 0 0 3 101 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 104 3 0.0 3 0 0 0 3 106 3 0.0 3 0 0 0 3 109 1 0.0 3 0 0 0 1 110 2 0.0 3 0 0 0 2 111 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 117 2 0.0 3 0 0 0 2 119 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 124 4 0.0 3 0 0 0 4 125 2 0.0 3 0 0 0 2 126 1 0.0 3 0 0 0 1 127 2 0.0 3 0 0 0 2 129 2 0.0 3 0 0 0 2 130 3 0.0 3 0 0 0 3 131 1 0.0 3 0 0 0 1 132 2 0.0 3 0 0 0 2 134 1 0.0 3 0 0 0 1 140 2 0.0 3 0 0 0 2 141 14 0.0 3 0 0 0 14 142 1 0.0 3 0 0 0 1 144 4 0.0 3 0 0 0 4 147 2 0.0 3 0 0 0 2 148 3 0.0 3 0 0 0 3 149 2 0.0 3 0 0 0 2 150 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 152 2 0.0 3 0 0 0 2 153 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 156 3 0.0 3 0 0 0 3 157 7 0.0 3 0 0 0 7 158 2 0.0 3 0 0 0 2 160 2 0.0 3 0 0 0 2 162 1 0.0 3 0 0 1 163 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 169 2 0.0 3 0 0 0 2 171 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 186 3 0.0 3 0 0 0 3 191 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 195 2 0.0 3 0 0 0 2 199 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 227 1 0.0 3 0 0 0 1 242 4 0.0 3 0 0 0 4 243 1 0.0 3 0 0 1 247 1 0.0 3 0 0 0 1 251 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 282 2 0.0 3 0 0 0 2 288 4 0.0 3 0 0 0 4 289 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.87 s (36 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 52,624 Reads with adapters: 2,155 (4.1%) Reads written (passing filters): 52,624 (100.0%) Total basepairs processed: 14,458,071 bp Total written (filtered): 14,451,556 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 2155 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 47.4% C: 6.0% G: 42.6% T: 4.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2110 822.2 0 2110 4 42 205.6 0 42 5 2 51.4 0 2 7 1 3.2 0 1