This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGTGGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 68.01 s (25 us/read; 2.39 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 134,983 (5.0%) Reads that were too short: 72,117 (2.7%) Reads written (passing filters): 62,866 (2.3%) Total basepairs processed: 815,312,680 bp Total written (filtered): 17,768,191 bp (2.2%) === Adapter 1 === Sequence: TGTGGC; Type: regular 5'; Length: 6; Trimmed: 134983 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2198 661.3 0 2198 7 51982 661.3 0 51982 8 88 661.3 0 88 9 40 661.3 0 40 10 42 661.3 0 42 11 62 661.3 0 62 12 234 661.3 0 234 13 35 661.3 0 35 14 50 661.3 0 50 15 41 661.3 0 41 16 44 661.3 0 44 17 55 661.3 0 55 18 52 661.3 0 52 19 51 661.3 0 51 20 40 661.3 0 40 21 41 661.3 0 41 22 38 661.3 0 38 23 28 661.3 0 28 24 46 661.3 0 46 25 40 661.3 0 40 26 41 661.3 0 41 27 34 661.3 0 34 28 47 661.3 0 47 29 48 661.3 0 48 30 52 661.3 0 52 31 34 661.3 0 34 32 71 661.3 0 71 33 69 661.3 0 69 34 55 661.3 0 55 35 42 661.3 0 42 36 46 661.3 0 46 37 60 661.3 0 60 38 61 661.3 0 61 39 58 661.3 0 58 40 56 661.3 0 56 41 58 661.3 0 58 42 51 661.3 0 51 43 61 661.3 0 61 44 40 661.3 0 40 45 55 661.3 0 55 46 44 661.3 0 44 47 57 661.3 0 57 48 40 661.3 0 40 49 50 661.3 0 50 50 54 661.3 0 54 51 43 661.3 0 43 52 564 661.3 0 564 53 84 661.3 0 84 54 75 661.3 0 75 55 53 661.3 0 53 56 37 661.3 0 37 57 46 661.3 0 46 58 62 661.3 0 62 59 33 661.3 0 33 60 42 661.3 0 42 61 56 661.3 0 56 62 49 661.3 0 49 63 57 661.3 0 57 64 52 661.3 0 52 65 48 661.3 0 48 66 61 661.3 0 61 67 53 661.3 0 53 68 40 661.3 0 40 69 43 661.3 0 43 70 59 661.3 0 59 71 84 661.3 0 84 72 64 661.3 0 64 73 93 661.3 0 93 74 28 661.3 0 28 75 36 661.3 0 36 76 27 661.3 0 27 77 59 661.3 0 59 78 30 661.3 0 30 79 35 661.3 0 35 80 37 661.3 0 37 81 36 661.3 0 36 82 39 661.3 0 39 83 28 661.3 0 28 84 37 661.3 0 37 85 21 661.3 0 21 86 36 661.3 0 36 87 22 661.3 0 22 88 28 661.3 0 28 89 38 661.3 0 38 90 38 661.3 0 38 91 32 661.3 0 32 92 32 661.3 0 32 93 40 661.3 0 40 94 37 661.3 0 37 95 27 661.3 0 27 96 48 661.3 0 48 97 32 661.3 0 32 98 26 661.3 0 26 99 30 661.3 0 30 100 39 661.3 0 39 101 31 661.3 0 31 102 27 661.3 0 27 103 41 661.3 0 41 104 17 661.3 0 17 105 31 661.3 0 31 106 27 661.3 0 27 107 19 661.3 0 19 108 24 661.3 0 24 109 35 661.3 0 35 110 37 661.3 0 37 111 36 661.3 0 36 112 22 661.3 0 22 113 33 661.3 0 33 114 27 661.3 0 27 115 18 661.3 0 18 116 33 661.3 0 33 117 59 661.3 0 59 118 31 661.3 0 31 119 25 661.3 0 25 120 55 661.3 0 55 121 33 661.3 0 33 122 20 661.3 0 20 123 36 661.3 0 36 124 147 661.3 0 147 125 19 661.3 0 19 126 31 661.3 0 31 127 53 661.3 0 53 128 23 661.3 0 23 129 22 661.3 0 22 130 62 661.3 0 62 131 168 661.3 0 168 132 439 661.3 0 439 133 60 661.3 0 60 134 35 661.3 0 35 135 20 661.3 0 20 136 24 661.3 0 24 137 108 661.3 0 108 138 124 661.3 0 124 139 31 661.3 0 31 140 30 661.3 0 30 141 24 661.3 0 24 142 33 661.3 0 33 143 29 661.3 0 29 144 22 661.3 0 22 145 37 661.3 0 37 146 25 661.3 0 25 147 25 661.3 0 25 148 33 661.3 0 33 149 23 661.3 0 23 150 20 661.3 0 20 151 39 661.3 0 39 152 55 661.3 0 55 153 83 661.3 0 83 154 22 661.3 0 22 155 34 661.3 0 34 156 19 661.3 0 19 157 28 661.3 0 28 158 28 661.3 0 28 159 23 661.3 0 23 160 15 661.3 0 15 161 14 661.3 0 14 162 23 661.3 0 23 163 22 661.3 0 22 164 19 661.3 0 19 165 19 661.3 0 19 166 16 661.3 0 16 167 20 661.3 0 20 168 21 661.3 0 21 169 30 661.3 0 30 170 18 661.3 0 18 171 15 661.3 0 15 172 12 661.3 0 12 173 29 661.3 0 29 174 18 661.3 0 18 175 20 661.3 0 20 176 38 661.3 0 38 177 34 661.3 0 34 178 49 661.3 0 49 179 25 661.3 0 25 180 15 661.3 0 15 181 23 661.3 0 23 182 20 661.3 0 20 183 22 661.3 0 22 184 18 661.3 0 18 185 16 661.3 0 16 186 36 661.3 0 36 187 21 661.3 0 21 188 27 661.3 0 27 189 31 661.3 0 31 190 22 661.3 0 22 191 16 661.3 0 16 192 17 661.3 0 17 193 25 661.3 0 25 194 16 661.3 0 16 195 18 661.3 0 18 196 24 661.3 0 24 197 32 661.3 0 32 198 21 661.3 0 21 199 20 661.3 0 20 200 23 661.3 0 23 201 24 661.3 0 24 202 39 661.3 0 39 203 217 661.3 0 217 204 156 661.3 0 156 205 112 661.3 0 112 206 23 661.3 0 23 207 27 661.3 0 27 208 24 661.3 0 24 209 25 661.3 0 25 210 25 661.3 0 25 211 32 661.3 0 32 212 114 661.3 0 114 213 180 661.3 0 180 214 156 661.3 0 156 215 24 661.3 0 24 216 28 661.3 0 28 217 17 661.3 0 17 218 29 661.3 0 29 219 32 661.3 0 32 220 44 661.3 0 44 221 11 661.3 0 11 222 23 661.3 0 23 223 16 661.3 0 16 224 22 661.3 0 22 225 66 661.3 0 66 226 34 661.3 0 34 227 23 661.3 0 23 228 70 661.3 0 70 229 21 661.3 0 21 230 19 661.3 0 19 231 20 661.3 0 20 232 37 661.3 0 37 233 28 661.3 0 28 234 39 661.3 0 39 235 108 661.3 0 108 236 345 661.3 0 345 237 306 661.3 0 306 238 307 661.3 0 307 239 30 661.3 0 30 240 22 661.3 0 22 241 21 661.3 0 21 242 16 661.3 0 16 243 43 661.3 0 43 244 49 661.3 0 49 245 78 661.3 0 78 246 60 661.3 0 60 247 44 661.3 0 44 248 23 661.3 0 23 249 32 661.3 0 32 250 38 661.3 0 38 251 31 661.3 0 31 252 37 661.3 0 37 253 44 661.3 0 44 254 46 661.3 0 46 255 49 661.3 0 49 256 70 661.3 0 70 257 62 661.3 0 62 258 66 661.3 0 66 259 64 661.3 0 64 260 308 661.3 0 308 261 237 661.3 0 237 262 2216 661.3 0 2216 263 197 661.3 0 197 264 224 661.3 0 224 265 200 661.3 0 200 266 55 661.3 0 55 267 61 661.3 0 61 268 93 661.3 0 93 269 112 661.3 0 112 270 106 661.3 0 106 271 102 661.3 0 102 272 216 661.3 0 216 273 571 661.3 0 571 274 164 661.3 0 164 275 221 661.3 0 221 276 486 661.3 0 486 277 240 661.3 0 240 278 189 661.3 0 189 279 472 661.3 0 472 280 402 661.3 0 402 281 377 661.3 0 377 282 300 661.3 0 300 283 327 661.3 0 327 284 414 661.3 0 414 285 647 661.3 0 647 286 8479 661.3 0 8479 287 19497 661.3 0 19497 288 30903 661.3 0 30903 289 155 661.3 0 155 290 24 661.3 0 24 291 28 661.3 0 28 292 31 661.3 0 31 293 17 661.3 0 17 294 17 661.3 0 17 295 84 661.3 0 84 296 67 661.3 0 67 297 48 661.3 0 48 298 35 661.3 0 35 299 25 661.3 0 25 300 33 661.3 0 33 301 13 661.3 0 13 Finished in 2.96 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 62,866 Reads with adapters: 53,584 (85.2%) Reads that were too short: 5 (0.0%) Reads written (passing filters): 53,579 (85.2%) Total basepairs processed: 17,768,191 bp Total written (filtered): 14,682,950 bp (82.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 53584 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 1 2 18 17 0.0 3 0 0 17 19 666 0.0 3 0 650 15 0 1 20 52754 0.0 4 52127 590 31 3 3 21 102 0.0 4 10 91 1 22 7 0.0 4 0 0 6 1 23 24 0.0 4 1 0 1 20 2 24 4 0.0 4 1 0 0 0 3 30 1 0.0 4 1 129 1 0.0 4 0 0 0 0 1 133 4 0.0 4 0 0 0 0 4 163 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.81 s (34 us/read; 1.78 M reads/minute). === Summary === Total reads processed: 53,579 Reads with adapters: 3,006 (5.6%) Reads written (passing filters): 53,579 (100.0%) Total basepairs processed: 14,682,950 bp Total written (filtered): 13,977,326 bp (95.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3006 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 27.9% C: 0.3% G: 13.0% T: 0.4% none/other: 58.3% Overview of removed sequences length count expect max.err error counts 3 9 837.2 0 9 4 1 209.3 0 1 10 3 0.1 1 0 3 11 381 0.0 1 1 380 12 13 0.0 1 0 13 20 1 0.0 1 1 140 1 0.0 1 0 1 210 1 0.0 1 0 1 225 3 0.0 1 3 259 1 0.0 1 1 260 3 0.0 1 3 261 413 0.0 1 391 22 262 385 0.0 1 347 38 263 29 0.0 1 27 2 271 5 0.0 1 4 1 272 2 0.0 1 2 273 9 0.0 1 8 1 274 1664 0.0 1 1598 66 275 82 0.0 1 79 3