This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCGGTT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 68.00 s (25 us/read; 2.39 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 423,022 (15.6%) Reads that were too short: 378,368 (14.0%) Reads written (passing filters): 44,654 (1.6%) Total basepairs processed: 815,312,680 bp Total written (filtered): 11,627,362 bp (1.4%) === Adapter 1 === Sequence: GCGGTT; Type: regular 5'; Length: 6; Trimmed: 423022 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 320 661.3 0 320 7 865 661.3 0 865 8 32247 661.3 0 32247 9 103 661.3 0 103 10 27 661.3 0 27 11 22 661.3 0 22 12 24 661.3 0 24 13 31 661.3 0 31 14 24 661.3 0 24 15 44 661.3 0 44 16 32 661.3 0 32 17 33 661.3 0 33 18 54 661.3 0 54 19 29 661.3 0 29 20 64 661.3 0 64 21 46 661.3 0 46 22 79 661.3 0 79 23 22 661.3 0 22 24 30 661.3 0 30 25 28 661.3 0 28 26 31 661.3 0 31 27 31 661.3 0 31 28 40 661.3 0 40 29 33 661.3 0 33 30 61 661.3 0 61 31 24 661.3 0 24 32 38 661.3 0 38 33 84 661.3 0 84 34 25 661.3 0 25 35 24 661.3 0 24 36 34 661.3 0 34 37 25 661.3 0 25 38 28 661.3 0 28 39 29 661.3 0 29 40 27 661.3 0 27 41 15 661.3 0 15 42 24 661.3 0 24 43 27 661.3 0 27 44 28 661.3 0 28 45 53 661.3 0 53 46 29 661.3 0 29 47 29 661.3 0 29 48 37 661.3 0 37 49 32 661.3 0 32 50 24 661.3 0 24 51 32 661.3 0 32 52 35 661.3 0 35 53 38 661.3 0 38 54 26 661.3 0 26 55 23 661.3 0 23 56 17 661.3 0 17 57 32 661.3 0 32 58 20 661.3 0 20 59 19 661.3 0 19 60 35 661.3 0 35 61 26 661.3 0 26 62 23 661.3 0 23 63 44 661.3 0 44 64 17 661.3 0 17 65 34 661.3 0 34 66 34 661.3 0 34 67 27 661.3 0 27 68 25 661.3 0 25 69 26 661.3 0 26 70 23 661.3 0 23 71 23 661.3 0 23 72 31 661.3 0 31 73 28 661.3 0 28 74 27 661.3 0 27 75 36 661.3 0 36 76 22 661.3 0 22 77 41 661.3 0 41 78 36 661.3 0 36 79 34 661.3 0 34 80 33 661.3 0 33 81 19 661.3 0 19 82 19 661.3 0 19 83 26 661.3 0 26 84 32 661.3 0 32 85 30 661.3 0 30 86 31 661.3 0 31 87 42 661.3 0 42 88 33 661.3 0 33 89 41 661.3 0 41 90 45 661.3 0 45 91 35 661.3 0 35 92 28 661.3 0 28 93 123 661.3 0 123 94 70 661.3 0 70 95 41 661.3 0 41 96 30 661.3 0 30 97 42 661.3 0 42 98 34 661.3 0 34 99 46 661.3 0 46 100 19 661.3 0 19 101 15 661.3 0 15 102 39 661.3 0 39 103 28 661.3 0 28 104 24 661.3 0 24 105 28 661.3 0 28 106 26 661.3 0 26 107 22 661.3 0 22 108 27 661.3 0 27 109 29 661.3 0 29 110 19 661.3 0 19 111 17 661.3 0 17 112 18 661.3 0 18 113 17 661.3 0 17 114 22 661.3 0 22 115 26 661.3 0 26 116 35 661.3 0 35 117 25 661.3 0 25 118 25 661.3 0 25 119 31 661.3 0 31 120 46 661.3 0 46 121 33 661.3 0 33 122 40 661.3 0 40 123 39 661.3 0 39 124 72 661.3 0 72 125 64 661.3 0 64 126 136 661.3 0 136 127 51 661.3 0 51 128 56 661.3 0 56 129 60 661.3 0 60 130 103 661.3 0 103 131 62 661.3 0 62 132 265 661.3 0 265 133 153 661.3 0 153 134 145 661.3 0 145 135 24 661.3 0 24 136 22 661.3 0 22 137 18 661.3 0 18 138 29 661.3 0 29 139 24 661.3 0 24 140 18 661.3 0 18 141 34 661.3 0 34 142 33 661.3 0 33 143 26 661.3 0 26 144 27 661.3 0 27 145 30 661.3 0 30 146 36 661.3 0 36 147 16 661.3 0 16 148 35 661.3 0 35 149 14 661.3 0 14 150 29 661.3 0 29 151 25 661.3 0 25 152 17 661.3 0 17 153 30 661.3 0 30 154 25 661.3 0 25 155 23 661.3 0 23 156 28 661.3 0 28 157 25 661.3 0 25 158 24 661.3 0 24 159 15 661.3 0 15 160 17 661.3 0 17 161 17 661.3 0 17 162 22 661.3 0 22 163 19 661.3 0 19 164 30 661.3 0 30 165 18 661.3 0 18 166 37 661.3 0 37 167 35 661.3 0 35 168 20 661.3 0 20 169 20 661.3 0 20 170 17 661.3 0 17 171 25 661.3 0 25 172 27 661.3 0 27 173 90 661.3 0 90 174 68 661.3 0 68 175 69 661.3 0 69 176 38 661.3 0 38 177 31 661.3 0 31 178 30 661.3 0 30 179 51 661.3 0 51 180 71 661.3 0 71 181 120 661.3 0 120 182 51 661.3 0 51 183 59 661.3 0 59 184 47 661.3 0 47 185 458 661.3 0 458 186 585 661.3 0 585 187 417 661.3 0 417 188 49 661.3 0 49 189 40 661.3 0 40 190 47 661.3 0 47 191 57 661.3 0 57 192 113 661.3 0 113 193 1062 661.3 0 1062 194 387 661.3 0 387 195 744 661.3 0 744 196 57 661.3 0 57 197 46 661.3 0 46 198 33 661.3 0 33 199 76 661.3 0 76 200 189 661.3 0 189 201 231 661.3 0 231 202 83 661.3 0 83 203 34 661.3 0 34 204 27 661.3 0 27 205 36 661.3 0 36 206 53 661.3 0 53 207 44 661.3 0 44 208 41 661.3 0 41 209 31 661.3 0 31 210 67 661.3 0 67 211 58 661.3 0 58 212 56 661.3 0 56 213 89 661.3 0 89 214 87 661.3 0 87 215 83 661.3 0 83 216 77 661.3 0 77 217 118 661.3 0 118 218 106 661.3 0 106 219 233 661.3 0 233 220 206 661.3 0 206 221 287 661.3 0 287 222 322 661.3 0 322 223 479 661.3 0 479 224 963 661.3 0 963 225 368 661.3 0 368 226 584 661.3 0 584 227 779 661.3 0 779 228 507 661.3 0 507 229 514 661.3 0 514 230 668 661.3 0 668 231 774 661.3 0 774 232 3143 661.3 0 3143 233 13859 661.3 0 13859 234 5095 661.3 0 5095 235 874 661.3 0 874 236 1097 661.3 0 1097 237 12260 661.3 0 12260 238 16006 661.3 0 16006 239 37823 661.3 0 37823 240 2978 661.3 0 2978 241 8712 661.3 0 8712 242 634 661.3 0 634 243 1030 661.3 0 1030 244 2066 661.3 0 2066 245 1038 661.3 0 1038 246 782 661.3 0 782 247 1307 661.3 0 1307 248 921 661.3 0 921 249 963 661.3 0 963 250 1570 661.3 0 1570 251 2360 661.3 0 2360 252 5293 661.3 0 5293 253 5063 661.3 0 5063 254 8576 661.3 0 8576 255 2157 661.3 0 2157 256 2622 661.3 0 2622 257 24525 661.3 0 24525 258 67240 661.3 0 67240 259 111275 661.3 0 111275 260 27665 661.3 0 27665 261 173 661.3 0 173 262 29 661.3 0 29 263 19 661.3 0 19 264 24 661.3 0 24 265 19 661.3 0 19 266 23 661.3 0 23 267 21 661.3 0 21 268 45 661.3 0 45 269 43 661.3 0 43 270 46 661.3 0 46 271 17 661.3 0 17 272 25 661.3 0 25 273 23 661.3 0 23 274 19 661.3 0 19 275 12 661.3 0 12 276 37 661.3 0 37 277 19 661.3 0 19 278 7 661.3 0 7 279 23 661.3 0 23 280 27 661.3 0 27 281 27 661.3 0 27 282 40 661.3 0 40 283 80 661.3 0 80 284 58 661.3 0 58 285 28 661.3 0 28 286 40 661.3 0 40 287 132 661.3 0 132 288 25 661.3 0 25 289 28 661.3 0 28 290 40 661.3 0 40 291 115 661.3 0 115 292 74 661.3 0 74 293 91 661.3 0 91 294 39 661.3 0 39 295 28 661.3 0 28 296 10 661.3 0 10 297 22 661.3 0 22 298 8 661.3 0 8 299 29 661.3 0 29 300 30 661.3 0 30 301 65 661.3 0 65 Finished in 1.95 s (44 us/read; 1.37 M reads/minute). === Summary === Total reads processed: 44,654 Reads with adapters: 33,288 (74.5%) Reads that were too short: 13 (0.0%) Reads written (passing filters): 33,275 (74.5%) Total basepairs processed: 11,627,362 bp Total written (filtered): 9,183,039 bp (79.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 33288 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 15 0.0 3 0 0 11 4 16 236 0.0 3 0 220 9 7 17 32502 0.0 3 31630 766 76 30 18 104 0.0 3 2 65 11 26 19 349 0.0 3 305 5 19 20 20 58 0.0 3 3 0 0 55 24 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 60 2 0.0 3 0 0 0 2 63 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 95 2 0.0 3 0 0 0 2 100 1 0.0 3 0 0 0 1 107 3 0.0 3 0 0 0 3 119 1 0.0 3 0 0 0 1 162 2 0.0 3 0 0 0 2 165 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 276 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.17 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 33,275 Reads with adapters: 888 (2.7%) Reads written (passing filters): 33,275 (100.0%) Total basepairs processed: 9,183,039 bp Total written (filtered): 9,180,331 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 888 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 65.9% C: 12.4% G: 13.3% T: 8.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 851 519.9 0 851 4 31 130.0 0 31 5 5 32.5 0 5 6 1 8.1 0 1