This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCGTTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 71.04 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 61,370 (2.3%) Reads that were too short: 14,999 (0.6%) Reads written (passing filters): 46,371 (1.7%) Total basepairs processed: 815,312,680 bp Total written (filtered): 11,824,102 bp (1.5%) === Adapter 1 === Sequence: TCGTTA; Type: regular 5'; Length: 6; Trimmed: 61370 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 214 661.3 0 214 7 307 661.3 0 307 8 867 661.3 0 867 9 30605 661.3 0 30605 10 77 661.3 0 77 11 29 661.3 0 29 12 26 661.3 0 26 13 29 661.3 0 29 14 26 661.3 0 26 15 17 661.3 0 17 16 37 661.3 0 37 17 25 661.3 0 25 18 29 661.3 0 29 19 25 661.3 0 25 20 38 661.3 0 38 21 33 661.3 0 33 22 18 661.3 0 18 23 39 661.3 0 39 24 31 661.3 0 31 25 29 661.3 0 29 26 15 661.3 0 15 27 22 661.3 0 22 28 31 661.3 0 31 29 45 661.3 0 45 30 28 661.3 0 28 31 19 661.3 0 19 32 33 661.3 0 33 33 35 661.3 0 35 34 42 661.3 0 42 35 28 661.3 0 28 36 31 661.3 0 31 37 24 661.3 0 24 38 57 661.3 0 57 39 29 661.3 0 29 40 60 661.3 0 60 41 59 661.3 0 59 42 41 661.3 0 41 43 25 661.3 0 25 44 36 661.3 0 36 45 21 661.3 0 21 46 31 661.3 0 31 47 42 661.3 0 42 48 36 661.3 0 36 49 49 661.3 0 49 50 34 661.3 0 34 51 32 661.3 0 32 52 43 661.3 0 43 53 27 661.3 0 27 54 28 661.3 0 28 55 29 661.3 0 29 56 29 661.3 0 29 57 32 661.3 0 32 58 28 661.3 0 28 59 32 661.3 0 32 60 55 661.3 0 55 61 43 661.3 0 43 62 40 661.3 0 40 63 29 661.3 0 29 64 47 661.3 0 47 65 55 661.3 0 55 66 72 661.3 0 72 67 44 661.3 0 44 68 47 661.3 0 47 69 44 661.3 0 44 70 38 661.3 0 38 71 48 661.3 0 48 72 43 661.3 0 43 73 55 661.3 0 55 74 86 661.3 0 86 75 36 661.3 0 36 76 57 661.3 0 57 77 31 661.3 0 31 78 44 661.3 0 44 79 42 661.3 0 42 80 50 661.3 0 50 81 34 661.3 0 34 82 30 661.3 0 30 83 35 661.3 0 35 84 44 661.3 0 44 85 33 661.3 0 33 86 34 661.3 0 34 87 36 661.3 0 36 88 32 661.3 0 32 89 41 661.3 0 41 90 26 661.3 0 26 91 59 661.3 0 59 92 34 661.3 0 34 93 50 661.3 0 50 94 144 661.3 0 144 95 128 661.3 0 128 96 61 661.3 0 61 97 226 661.3 0 226 98 245 661.3 0 245 99 186 661.3 0 186 100 224 661.3 0 224 101 52 661.3 0 52 102 39 661.3 0 39 103 39 661.3 0 39 104 57 661.3 0 57 105 36 661.3 0 36 106 33 661.3 0 33 107 40 661.3 0 40 108 49 661.3 0 49 109 70 661.3 0 70 110 63 661.3 0 63 111 261 661.3 0 261 112 679 661.3 0 679 113 403 661.3 0 403 114 36 661.3 0 36 115 38 661.3 0 38 116 47 661.3 0 47 117 48 661.3 0 48 118 27 661.3 0 27 119 53 661.3 0 53 120 50 661.3 0 50 121 46 661.3 0 46 122 60 661.3 0 60 123 43 661.3 0 43 124 51 661.3 0 51 125 60 661.3 0 60 126 65 661.3 0 65 127 56 661.3 0 56 128 220 661.3 0 220 129 157 661.3 0 157 130 56 661.3 0 56 131 121 661.3 0 121 132 182 661.3 0 182 133 289 661.3 0 289 134 174 661.3 0 174 135 98 661.3 0 98 136 107 661.3 0 107 137 45 661.3 0 45 138 54 661.3 0 54 139 48 661.3 0 48 140 62 661.3 0 62 141 55 661.3 0 55 142 41 661.3 0 41 143 35 661.3 0 35 144 40 661.3 0 40 145 57 661.3 0 57 146 80 661.3 0 80 147 55 661.3 0 55 148 49 661.3 0 49 149 52 661.3 0 52 150 45 661.3 0 45 151 52 661.3 0 52 152 38 661.3 0 38 153 36 661.3 0 36 154 43 661.3 0 43 155 123 661.3 0 123 156 134 661.3 0 134 157 73 661.3 0 73 158 49 661.3 0 49 159 48 661.3 0 48 160 42 661.3 0 42 161 56 661.3 0 56 162 34 661.3 0 34 163 34 661.3 0 34 164 56 661.3 0 56 165 45 661.3 0 45 166 101 661.3 0 101 167 59 661.3 0 59 168 63 661.3 0 63 169 42 661.3 0 42 170 49 661.3 0 49 171 41 661.3 0 41 172 40 661.3 0 40 173 47 661.3 0 47 174 98 661.3 0 98 175 82 661.3 0 82 176 67 661.3 0 67 177 59 661.3 0 59 178 83 661.3 0 83 179 213 661.3 0 213 180 136 661.3 0 136 181 185 661.3 0 185 182 76 661.3 0 76 183 70 661.3 0 70 184 73 661.3 0 73 185 86 661.3 0 86 186 79 661.3 0 79 187 72 661.3 0 72 188 61 661.3 0 61 189 51 661.3 0 51 190 68 661.3 0 68 191 58 661.3 0 58 192 82 661.3 0 82 193 192 661.3 0 192 194 715 661.3 0 715 195 352 661.3 0 352 196 474 661.3 0 474 197 67 661.3 0 67 198 94 661.3 0 94 199 159 661.3 0 159 200 168 661.3 0 168 201 161 661.3 0 161 202 117 661.3 0 117 203 55 661.3 0 55 204 85 661.3 0 85 205 83 661.3 0 83 206 71 661.3 0 71 207 93 661.3 0 93 208 70 661.3 0 70 209 71 661.3 0 71 210 62 661.3 0 62 211 64 661.3 0 64 212 76 661.3 0 76 213 65 661.3 0 65 214 66 661.3 0 66 215 110 661.3 0 110 216 115 661.3 0 115 217 152 661.3 0 152 218 55 661.3 0 55 219 67 661.3 0 67 220 317 661.3 0 317 221 267 661.3 0 267 222 268 661.3 0 268 223 134 661.3 0 134 224 75 661.3 0 75 225 45 661.3 0 45 226 71 661.3 0 71 227 90 661.3 0 90 228 72 661.3 0 72 229 88 661.3 0 88 230 58 661.3 0 58 231 54 661.3 0 54 232 42 661.3 0 42 233 61 661.3 0 61 234 57 661.3 0 57 235 85 661.3 0 85 236 70 661.3 0 70 237 321 661.3 0 321 238 595 661.3 0 595 239 814 661.3 0 814 240 78 661.3 0 78 241 106 661.3 0 106 242 114 661.3 0 114 243 137 661.3 0 137 244 189 661.3 0 189 245 89 661.3 0 89 246 81 661.3 0 81 247 75 661.3 0 75 248 66 661.3 0 66 249 107 661.3 0 107 250 135 661.3 0 135 251 71 661.3 0 71 252 189 661.3 0 189 253 108 661.3 0 108 254 269 661.3 0 269 255 185 661.3 0 185 256 285 661.3 0 285 257 162 661.3 0 162 258 127 661.3 0 127 259 106 661.3 0 106 260 88 661.3 0 88 261 77 661.3 0 77 262 466 661.3 0 466 263 958 661.3 0 958 264 2009 661.3 0 2009 265 82 661.3 0 82 266 89 661.3 0 89 267 65 661.3 0 65 268 312 661.3 0 312 269 360 661.3 0 360 270 406 661.3 0 406 271 60 661.3 0 60 272 55 661.3 0 55 273 64 661.3 0 64 274 56 661.3 0 56 275 51 661.3 0 51 276 63 661.3 0 63 277 52 661.3 0 52 278 40 661.3 0 40 279 64 661.3 0 64 280 75 661.3 0 75 281 92 661.3 0 92 282 75 661.3 0 75 283 66 661.3 0 66 284 59 661.3 0 59 285 80 661.3 0 80 286 85 661.3 0 85 287 72 661.3 0 72 288 52 661.3 0 52 289 46 661.3 0 46 290 43 661.3 0 43 291 64 661.3 0 64 292 59 661.3 0 59 293 120 661.3 0 120 294 510 661.3 0 510 295 99 661.3 0 99 296 37 661.3 0 37 297 22 661.3 0 22 298 36 661.3 0 36 299 24 661.3 0 24 300 44 661.3 0 44 301 82 661.3 0 82 Finished in 2.12 s (46 us/read; 1.31 M reads/minute). === Summary === Total reads processed: 46,371 Reads with adapters: 31,912 (68.8%) Reads that were too short: 6 (0.0%) Reads written (passing filters): 31,906 (68.8%) Total basepairs processed: 11,824,102 bp Total written (filtered): 8,680,234 bp (73.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 31912 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 7 0.0 3 0 0 0 0 7 18 13 0.0 3 0 0 12 1 19 483 0.0 3 0 472 9 1 1 20 31265 0.0 4 30824 427 9 2 3 21 87 0.0 4 12 73 1 1 22 6 0.0 4 1 0 5 23 21 0.0 4 3 0 0 16 2 24 20 0.0 4 0 0 0 1 19 52 1 0.0 4 0 0 0 0 1 53 1 0.0 4 1 61 1 0.0 4 0 0 0 1 82 1 0.0 4 0 0 0 0 1 93 1 0.0 4 0 0 0 0 1 122 1 0.0 4 0 0 0 0 1 131 1 0.0 4 0 0 0 0 1 148 1 0.0 4 0 0 0 0 1 176 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.09 s (34 us/read; 1.76 M reads/minute). === Summary === Total reads processed: 31,906 Reads with adapters: 7,140 (22.4%) Reads written (passing filters): 31,906 (100.0%) Total basepairs processed: 8,680,234 bp Total written (filtered): 7,004,640 bp (80.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 7140 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.2% C: 0.3% G: 99.4% T: 0.1% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1 498.5 0 1 4 5 124.6 0 5 6 1 7.8 0 1 8 1 0.5 0 1 9 68 0.1 0 3 65 10 515 0.0 1 15 500 11 158 0.0 1 1 157 12 1 0.0 1 0 1 150 1 0.0 1 0 1 208 3 0.0 1 2 1 257 1 0.0 1 0 1 258 3 0.0 1 1 2 259 1 0.0 1 1 260 25 0.0 1 24 1 261 5995 0.0 1 5748 247 262 243 0.0 1 229 14 263 49 0.0 1 45 4 264 63 0.0 1 57 6 265 1 0.0 1 1 266 4 0.0 1 4 272 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.