This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCACT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 72.37 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 226,189 (8.4%) Reads that were too short: 37,837 (1.4%) Reads written (passing filters): 188,352 (7.0%) Total basepairs processed: 815,312,680 bp Total written (filtered): 36,998,325 bp (4.5%) === Adapter 1 === Sequence: AGCACT; Type: regular 5'; Length: 6; Trimmed: 226189 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 313 661.3 0 313 7 14596 661.3 0 14596 8 18404 661.3 0 18404 9 91 661.3 0 91 10 26 661.3 0 26 11 43 661.3 0 43 12 106 661.3 0 106 13 52 661.3 0 52 14 229 661.3 0 229 15 26 661.3 0 26 16 27 661.3 0 27 17 25 661.3 0 25 18 37 661.3 0 37 19 27 661.3 0 27 20 24 661.3 0 24 21 34 661.3 0 34 22 41 661.3 0 41 23 22 661.3 0 22 24 37 661.3 0 37 25 71 661.3 0 71 26 79 661.3 0 79 27 133 661.3 0 133 28 32 661.3 0 32 29 37 661.3 0 37 30 45 661.3 0 45 31 37 661.3 0 37 32 40 661.3 0 40 33 40 661.3 0 40 34 30 661.3 0 30 35 63 661.3 0 63 36 39 661.3 0 39 37 52 661.3 0 52 38 29 661.3 0 29 39 19 661.3 0 19 40 64 661.3 0 64 41 42 661.3 0 42 42 25 661.3 0 25 43 75 661.3 0 75 44 43 661.3 0 43 45 22 661.3 0 22 46 30 661.3 0 30 47 40 661.3 0 40 48 45 661.3 0 45 49 43 661.3 0 43 50 45 661.3 0 45 51 34 661.3 0 34 52 36 661.3 0 36 53 35 661.3 0 35 54 51 661.3 0 51 55 47 661.3 0 47 56 106 661.3 0 106 57 35 661.3 0 35 58 37 661.3 0 37 59 29 661.3 0 29 60 44 661.3 0 44 61 38 661.3 0 38 62 37 661.3 0 37 63 31 661.3 0 31 64 44 661.3 0 44 65 163 661.3 0 163 66 47 661.3 0 47 67 88 661.3 0 88 68 56 661.3 0 56 69 42 661.3 0 42 70 34 661.3 0 34 71 21 661.3 0 21 72 48 661.3 0 48 73 49 661.3 0 49 74 60 661.3 0 60 75 49 661.3 0 49 76 44 661.3 0 44 77 49 661.3 0 49 78 47 661.3 0 47 79 95 661.3 0 95 80 92 661.3 0 92 81 154 661.3 0 154 82 110 661.3 0 110 83 97 661.3 0 97 84 127 661.3 0 127 85 95 661.3 0 95 86 185 661.3 0 185 87 318 661.3 0 318 88 313 661.3 0 313 89 287 661.3 0 287 90 459 661.3 0 459 91 795 661.3 0 795 92 371 661.3 0 371 93 435 661.3 0 435 94 590 661.3 0 590 95 366 661.3 0 366 96 297 661.3 0 297 97 397 661.3 0 397 98 473 661.3 0 473 99 507 661.3 0 507 100 460 661.3 0 460 101 574 661.3 0 574 102 721 661.3 0 721 103 937 661.3 0 937 104 12939 661.3 0 12939 105 29848 661.3 0 29848 106 37772 661.3 0 37772 107 208 661.3 0 208 108 52 661.3 0 52 109 53 661.3 0 53 110 114 661.3 0 114 111 176 661.3 0 176 112 137 661.3 0 137 113 173 661.3 0 173 114 135 661.3 0 135 115 67 661.3 0 67 116 109 661.3 0 109 117 97 661.3 0 97 118 111 661.3 0 111 119 231 661.3 0 231 120 213 661.3 0 213 121 191 661.3 0 191 122 122 661.3 0 122 123 131 661.3 0 131 124 141 661.3 0 141 125 210 661.3 0 210 126 284 661.3 0 284 127 232 661.3 0 232 128 167 661.3 0 167 129 144 661.3 0 144 130 133 661.3 0 133 131 108 661.3 0 108 132 187 661.3 0 187 133 149 661.3 0 149 134 144 661.3 0 144 135 125 661.3 0 125 136 126 661.3 0 126 137 115 661.3 0 115 138 233 661.3 0 233 139 255 661.3 0 255 140 230 661.3 0 230 141 158 661.3 0 158 142 361 661.3 0 361 143 182 661.3 0 182 144 234 661.3 0 234 145 221 661.3 0 221 146 243 661.3 0 243 147 300 661.3 0 300 148 170 661.3 0 170 149 178 661.3 0 178 150 134 661.3 0 134 151 233 661.3 0 233 152 333 661.3 0 333 153 329 661.3 0 329 154 318 661.3 0 318 155 435 661.3 0 435 156 450 661.3 0 450 157 734 661.3 0 734 158 1260 661.3 0 1260 159 19825 661.3 0 19825 160 3550 661.3 0 3550 161 14934 661.3 0 14934 162 244 661.3 0 244 163 226 661.3 0 226 164 182 661.3 0 182 165 164 661.3 0 164 166 161 661.3 0 161 167 383 661.3 0 383 168 240 661.3 0 240 169 364 661.3 0 364 170 124 661.3 0 124 171 187 661.3 0 187 172 286 661.3 0 286 173 280 661.3 0 280 174 296 661.3 0 296 175 172 661.3 0 172 176 143 661.3 0 143 177 159 661.3 0 159 178 191 661.3 0 191 179 221 661.3 0 221 180 242 661.3 0 242 181 284 661.3 0 284 182 204 661.3 0 204 183 241 661.3 0 241 184 444 661.3 0 444 185 502 661.3 0 502 186 348 661.3 0 348 187 268 661.3 0 268 188 223 661.3 0 223 189 376 661.3 0 376 190 219 661.3 0 219 191 188 661.3 0 188 192 177 661.3 0 177 193 119 661.3 0 119 194 165 661.3 0 165 195 202 661.3 0 202 196 168 661.3 0 168 197 162 661.3 0 162 198 200 661.3 0 200 199 268 661.3 0 268 200 2301 661.3 0 2301 201 548 661.3 0 548 202 404 661.3 0 404 203 196 661.3 0 196 204 185 661.3 0 185 205 209 661.3 0 209 206 285 661.3 0 285 207 250 661.3 0 250 208 185 661.3 0 185 209 172 661.3 0 172 210 169 661.3 0 169 211 176 661.3 0 176 212 197 661.3 0 197 213 221 661.3 0 221 214 204 661.3 0 204 215 333 661.3 0 333 216 207 661.3 0 207 217 220 661.3 0 220 218 225 661.3 0 225 219 367 661.3 0 367 220 425 661.3 0 425 221 394 661.3 0 394 222 186 661.3 0 186 223 208 661.3 0 208 224 267 661.3 0 267 225 228 661.3 0 228 226 274 661.3 0 274 227 231 661.3 0 231 228 378 661.3 0 378 229 308 661.3 0 308 230 304 661.3 0 304 231 209 661.3 0 209 232 206 661.3 0 206 233 205 661.3 0 205 234 164 661.3 0 164 235 145 661.3 0 145 236 257 661.3 0 257 237 332 661.3 0 332 238 267 661.3 0 267 239 457 661.3 0 457 240 6147 661.3 0 6147 241 1944 661.3 0 1944 242 1131 661.3 0 1131 243 565 661.3 0 565 244 259 661.3 0 259 245 166 661.3 0 166 246 173 661.3 0 173 247 199 661.3 0 199 248 186 661.3 0 186 249 246 661.3 0 246 250 234 661.3 0 234 251 248 661.3 0 248 252 251 661.3 0 251 253 415 661.3 0 415 254 361 661.3 0 361 255 360 661.3 0 360 256 263 661.3 0 263 257 204 661.3 0 204 258 161 661.3 0 161 259 119 661.3 0 119 260 141 661.3 0 141 261 115 661.3 0 115 262 136 661.3 0 136 263 235 661.3 0 235 264 245 661.3 0 245 265 302 661.3 0 302 266 226 661.3 0 226 267 208 661.3 0 208 268 375 661.3 0 375 269 349 661.3 0 349 270 230 661.3 0 230 271 221 661.3 0 221 272 277 661.3 0 277 273 264 661.3 0 264 274 199 661.3 0 199 275 415 661.3 0 415 276 337 661.3 0 337 277 212 661.3 0 212 278 436 661.3 0 436 279 369 661.3 0 369 280 385 661.3 0 385 281 810 661.3 0 810 282 1534 661.3 0 1534 283 2170 661.3 0 2170 284 330 661.3 0 330 285 179 661.3 0 179 286 154 661.3 0 154 287 172 661.3 0 172 288 215 661.3 0 215 289 196 661.3 0 196 290 334 661.3 0 334 291 244 661.3 0 244 292 266 661.3 0 266 293 247 661.3 0 247 294 256 661.3 0 256 295 170 661.3 0 170 296 174 661.3 0 174 297 179 661.3 0 179 298 153 661.3 0 153 299 303 661.3 0 303 300 579 661.3 0 579 301 213 661.3 0 213 Finished in 6.68 s (35 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 188,352 Reads with adapters: 14,545 (7.7%) Reads that were too short: 162 (0.1%) Reads written (passing filters): 14,383 (7.6%) Total basepairs processed: 36,998,325 bp Total written (filtered): 3,960,883 bp (10.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 14545 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 2 0.0 3 0 0 2 16 115 0.0 3 0 108 3 4 17 14038 0.0 3 13731 277 18 12 18 49 0.0 3 1 40 4 4 19 15 0.0 3 1 0 9 5 20 21 0.0 3 0 0 0 21 21 1 0.0 3 1 23 1 0.0 3 0 0 0 1 24 1 0.0 3 0 0 0 1 28 1 0.0 3 1 32 2 0.0 3 0 0 0 2 33 2 0.0 3 0 0 0 2 39 1 0.0 3 0 0 0 1 40 2 0.0 3 0 0 0 2 44 2 0.0 3 0 0 0 2 46 8 0.0 3 0 0 0 8 47 1 0.0 3 0 0 0 1 49 2 0.0 3 0 0 0 2 54 3 0.0 3 0 0 0 3 58 1 0.0 3 0 0 0 1 59 2 0.0 3 0 0 0 2 63 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 78 2 0.0 3 0 0 0 2 81 1 0.0 3 0 0 0 1 83 1 0.0 3 1 84 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 86 2 0.0 3 0 0 0 2 87 31 0.0 3 0 0 0 31 88 41 0.0 3 0 0 0 41 90 11 0.0 3 0 0 2 9 91 8 0.0 3 0 0 0 8 95 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 99 2 0.0 3 0 0 0 2 100 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 111 3 0.0 3 0 0 0 3 114 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 126 2 0.0 3 0 0 0 2 128 2 0.0 3 0 0 0 2 130 9 0.0 3 0 0 0 9 131 11 0.0 3 0 0 0 11 132 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 137 3 0.0 3 0 0 0 3 138 2 0.0 3 0 0 0 2 139 3 0.0 3 0 0 0 3 140 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 153 2 0.0 3 0 0 1 1 154 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 163 2 0.0 3 0 0 0 2 164 3 0.0 3 0 0 0 3 174 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 178 3 0.0 3 0 0 0 3 187 1 0.0 3 0 0 0 1 188 4 0.0 3 0 0 0 4 189 76 0.0 3 0 0 3 73 190 3 0.0 3 0 0 0 3 192 5 0.0 3 0 0 0 5 194 1 0.0 3 0 0 0 1 199 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 219 1 0.0 3 0 0 0 1 270 2 0.0 3 0 0 0 2 281 3 0.0 3 0 0 0 3 282 2 0.0 3 0 0 1 1 283 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.51 s (35 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 14,383 Reads with adapters: 692 (4.8%) Reads written (passing filters): 14,383 (100.0%) Total basepairs processed: 3,960,883 bp Total written (filtered): 3,958,798 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 692 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 26.6% C: 57.5% G: 11.6% T: 4.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 685 224.7 0 685 4 5 56.2 0 5 5 2 14.0 0 2