This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCCTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 73.45 s (27 us/read; 2.21 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 237,082 (8.8%) Reads that were too short: 10,705 (0.4%) Reads written (passing filters): 226,377 (8.4%) Total basepairs processed: 815,312,680 bp Total written (filtered): 53,356,466 bp (6.5%) === Adapter 1 === Sequence: AGCCTC; Type: regular 5'; Length: 6; Trimmed: 237082 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 383 661.3 0 383 7 536 661.3 0 536 8 13677 661.3 0 13677 9 115 661.3 0 115 10 76 661.3 0 76 11 72 661.3 0 72 12 203 661.3 0 203 13 86 661.3 0 86 14 82 661.3 0 82 15 91 661.3 0 91 16 103 661.3 0 103 17 63 661.3 0 63 18 94 661.3 0 94 19 87 661.3 0 87 20 113 661.3 0 113 21 101 661.3 0 101 22 90 661.3 0 90 23 113 661.3 0 113 24 111 661.3 0 111 25 101 661.3 0 101 26 91 661.3 0 91 27 85 661.3 0 85 28 80 661.3 0 80 29 245 661.3 0 245 30 101 661.3 0 101 31 65 661.3 0 65 32 78 661.3 0 78 33 78 661.3 0 78 34 77 661.3 0 77 35 97 661.3 0 97 36 120 661.3 0 120 37 126 661.3 0 126 38 187 661.3 0 187 39 490 661.3 0 490 40 131 661.3 0 131 41 248 661.3 0 248 42 126 661.3 0 126 43 296 661.3 0 296 44 607 661.3 0 607 45 192 661.3 0 192 46 193 661.3 0 193 47 133 661.3 0 133 48 132 661.3 0 132 49 207 661.3 0 207 50 383 661.3 0 383 51 569 661.3 0 569 52 792 661.3 0 792 53 1235 661.3 0 1235 54 2240 661.3 0 2240 55 8474 661.3 0 8474 56 100999 661.3 0 100999 57 10501 661.3 0 10501 58 51651 661.3 0 51651 59 264 661.3 0 264 60 83 661.3 0 83 61 74 661.3 0 74 62 86 661.3 0 86 63 83 661.3 0 83 64 78 661.3 0 78 65 52 661.3 0 52 66 72 661.3 0 72 67 80 661.3 0 80 68 150 661.3 0 150 69 62 661.3 0 62 70 62 661.3 0 62 71 72 661.3 0 72 72 69 661.3 0 69 73 56 661.3 0 56 74 62 661.3 0 62 75 86 661.3 0 86 76 42 661.3 0 42 77 52 661.3 0 52 78 68 661.3 0 68 79 56 661.3 0 56 80 65 661.3 0 65 81 84 661.3 0 84 82 76 661.3 0 76 83 153 661.3 0 153 84 117 661.3 0 117 85 78 661.3 0 78 86 65 661.3 0 65 87 205 661.3 0 205 88 131 661.3 0 131 89 161 661.3 0 161 90 72 661.3 0 72 91 59 661.3 0 59 92 67 661.3 0 67 93 104 661.3 0 104 94 65 661.3 0 65 95 53 661.3 0 53 96 93 661.3 0 93 97 68 661.3 0 68 98 55 661.3 0 55 99 61 661.3 0 61 100 80 661.3 0 80 101 81 661.3 0 81 102 64 661.3 0 64 103 92 661.3 0 92 104 108 661.3 0 108 105 131 661.3 0 131 106 53 661.3 0 53 107 67 661.3 0 67 108 73 661.3 0 73 109 81 661.3 0 81 110 58 661.3 0 58 111 77 661.3 0 77 112 44 661.3 0 44 113 50 661.3 0 50 114 95 661.3 0 95 115 60 661.3 0 60 116 66 661.3 0 66 117 75 661.3 0 75 118 80 661.3 0 80 119 114 661.3 0 114 120 153 661.3 0 153 121 83 661.3 0 83 122 68 661.3 0 68 123 109 661.3 0 109 124 733 661.3 0 733 125 909 661.3 0 909 126 1160 661.3 0 1160 127 268 661.3 0 268 128 88 661.3 0 88 129 72 661.3 0 72 130 77 661.3 0 77 131 74 661.3 0 74 132 207 661.3 0 207 133 91 661.3 0 91 134 85 661.3 0 85 135 115 661.3 0 115 136 60 661.3 0 60 137 62 661.3 0 62 138 90 661.3 0 90 139 75 661.3 0 75 140 71 661.3 0 71 141 81 661.3 0 81 142 102 661.3 0 102 143 94 661.3 0 94 144 94 661.3 0 94 145 117 661.3 0 117 146 165 661.3 0 165 147 1207 661.3 0 1207 148 291 661.3 0 291 149 440 661.3 0 440 150 785 661.3 0 785 151 252 661.3 0 252 152 229 661.3 0 229 153 82 661.3 0 82 154 54 661.3 0 54 155 87 661.3 0 87 156 145 661.3 0 145 157 99 661.3 0 99 158 98 661.3 0 98 159 81 661.3 0 81 160 174 661.3 0 174 161 125 661.3 0 125 162 122 661.3 0 122 163 104 661.3 0 104 164 95 661.3 0 95 165 123 661.3 0 123 166 200 661.3 0 200 167 1586 661.3 0 1586 168 472 661.3 0 472 169 428 661.3 0 428 170 241 661.3 0 241 171 140 661.3 0 140 172 179 661.3 0 179 173 222 661.3 0 222 174 172 661.3 0 172 175 198 661.3 0 198 176 254 661.3 0 254 177 284 661.3 0 284 178 98 661.3 0 98 179 102 661.3 0 102 180 134 661.3 0 134 181 101 661.3 0 101 182 81 661.3 0 81 183 120 661.3 0 120 184 85 661.3 0 85 185 80 661.3 0 80 186 119 661.3 0 119 187 99 661.3 0 99 188 152 661.3 0 152 189 111 661.3 0 111 190 92 661.3 0 92 191 136 661.3 0 136 192 185 661.3 0 185 193 114 661.3 0 114 194 129 661.3 0 129 195 327 661.3 0 327 196 316 661.3 0 316 197 397 661.3 0 397 198 288 661.3 0 288 199 695 661.3 0 695 200 4252 661.3 0 4252 201 1476 661.3 0 1476 202 2385 661.3 0 2385 203 153 661.3 0 153 204 57 661.3 0 57 205 70 661.3 0 70 206 73 661.3 0 73 207 84 661.3 0 84 208 73 661.3 0 73 209 72 661.3 0 72 210 75 661.3 0 75 211 67 661.3 0 67 212 77 661.3 0 77 213 98 661.3 0 98 214 91 661.3 0 91 215 67 661.3 0 67 216 74 661.3 0 74 217 78 661.3 0 78 218 68 661.3 0 68 219 95 661.3 0 95 220 103 661.3 0 103 221 106 661.3 0 106 222 73 661.3 0 73 223 63 661.3 0 63 224 90 661.3 0 90 225 76 661.3 0 76 226 63 661.3 0 63 227 86 661.3 0 86 228 99 661.3 0 99 229 271 661.3 0 271 230 108 661.3 0 108 231 135 661.3 0 135 232 207 661.3 0 207 233 191 661.3 0 191 234 173 661.3 0 173 235 71 661.3 0 71 236 62 661.3 0 62 237 53 661.3 0 53 238 70 661.3 0 70 239 94 661.3 0 94 240 71 661.3 0 71 241 92 661.3 0 92 242 134 661.3 0 134 243 77 661.3 0 77 244 72 661.3 0 72 245 60 661.3 0 60 246 64 661.3 0 64 247 56 661.3 0 56 248 63 661.3 0 63 249 64 661.3 0 64 250 54 661.3 0 54 251 64 661.3 0 64 252 79 661.3 0 79 253 85 661.3 0 85 254 72 661.3 0 72 255 71 661.3 0 71 256 84 661.3 0 84 257 53 661.3 0 53 258 79 661.3 0 79 259 55 661.3 0 55 260 65 661.3 0 65 261 76 661.3 0 76 262 75 661.3 0 75 263 88 661.3 0 88 264 64 661.3 0 64 265 98 661.3 0 98 266 77 661.3 0 77 267 58 661.3 0 58 268 57 661.3 0 57 269 69 661.3 0 69 270 60 661.3 0 60 271 61 661.3 0 61 272 78 661.3 0 78 273 97 661.3 0 97 274 103 661.3 0 103 275 107 661.3 0 107 276 99 661.3 0 99 277 79 661.3 0 79 278 90 661.3 0 90 279 89 661.3 0 89 280 78 661.3 0 78 281 80 661.3 0 80 282 96 661.3 0 96 283 117 661.3 0 117 284 87 661.3 0 87 285 64 661.3 0 64 286 54 661.3 0 54 287 70 661.3 0 70 288 57 661.3 0 57 289 71 661.3 0 71 290 63 661.3 0 63 291 97 661.3 0 97 292 144 661.3 0 144 293 110 661.3 0 110 294 51 661.3 0 51 295 41 661.3 0 41 296 47 661.3 0 47 297 42 661.3 0 42 298 59 661.3 0 59 299 62 661.3 0 62 300 118 661.3 0 118 301 107 661.3 0 107 Finished in 9.85 s (44 us/read; 1.38 M reads/minute). === Summary === Total reads processed: 226,377 Reads with adapters: 14,384 (6.4%) Reads that were too short: 5 (0.0%) Reads written (passing filters): 14,379 (6.4%) Total basepairs processed: 53,356,466 bp Total written (filtered): 3,925,465 bp (7.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 14384 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 2 1 18 19 0.0 3 0 0 12 1 6 19 168 0.0 3 0 163 3 1 1 20 13936 0.0 4 13784 142 7 1 2 21 44 0.0 4 6 36 1 0 1 22 3 0.0 4 1 1 1 23 201 0.0 4 195 2 0 3 1 24 2 0.0 4 0 0 0 0 2 66 1 0.0 4 0 0 0 0 1 71 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 0 1 103 1 0.0 4 0 0 0 0 1 122 1 0.0 4 0 0 0 0 1 149 1 0.0 4 0 1 172 1 0.0 4 1 202 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.49 s (34 us/read; 1.76 M reads/minute). === Summary === Total reads processed: 14,379 Reads with adapters: 632 (4.4%) Reads written (passing filters): 14,379 (100.0%) Total basepairs processed: 3,925,465 bp Total written (filtered): 3,907,517 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 632 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 5.4% C: 1.6% G: 90.3% T: 2.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 3 224.7 0 3 4 1 56.2 0 1 10 73 0.0 1 4 69 11 506 0.0 1 1 505 12 1 0.0 1 0 1 19 1 0.0 1 1 20 1 0.0 1 1 21 2 0.0 1 1 1 256 1 0.0 1 1 258 1 0.0 1 1 260 27 0.0 1 27 266 1 0.0 1 1 267 10 0.0 1 7 3 268 2 0.0 1 2 269 1 0.0 1 0 1 271 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.