This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTAGTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 71.34 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 103,548 (3.8%) Reads that were too short: 10,055 (0.4%) Reads written (passing filters): 93,493 (3.5%) Total basepairs processed: 815,312,680 bp Total written (filtered): 24,761,015 bp (3.0%) === Adapter 1 === Sequence: GTAGTG; Type: regular 5'; Length: 6; Trimmed: 103548 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1076 661.3 0 1076 7 41592 661.3 0 41592 8 726 661.3 0 726 9 25409 661.3 0 25409 10 50 661.3 0 50 11 33 661.3 0 33 12 16 661.3 0 16 13 13 661.3 0 13 14 17 661.3 0 17 15 19 661.3 0 19 16 24 661.3 0 24 17 1081 661.3 0 1081 18 177 661.3 0 177 19 14 661.3 0 14 20 22 661.3 0 22 21 17 661.3 0 17 22 145 661.3 0 145 23 66 661.3 0 66 24 12 661.3 0 12 25 23 661.3 0 23 26 31 661.3 0 31 27 43 661.3 0 43 28 108 661.3 0 108 29 115 661.3 0 115 30 834 661.3 0 834 31 157 661.3 0 157 32 14 661.3 0 14 33 26 661.3 0 26 34 15 661.3 0 15 35 28 661.3 0 28 36 23 661.3 0 23 37 12 661.3 0 12 38 16 661.3 0 16 39 16 661.3 0 16 40 10 661.3 0 10 41 12 661.3 0 12 42 25 661.3 0 25 43 16 661.3 0 16 44 16 661.3 0 16 45 34 661.3 0 34 46 16 661.3 0 16 47 12 661.3 0 12 48 26 661.3 0 26 49 15 661.3 0 15 50 24 661.3 0 24 51 216 661.3 0 216 52 24 661.3 0 24 53 21 661.3 0 21 54 13 661.3 0 13 55 18 661.3 0 18 56 11 661.3 0 11 57 15 661.3 0 15 58 26 661.3 0 26 59 16 661.3 0 16 60 19 661.3 0 19 61 21 661.3 0 21 62 19 661.3 0 19 63 21 661.3 0 21 64 20 661.3 0 20 65 19 661.3 0 19 66 23 661.3 0 23 67 37 661.3 0 37 68 44 661.3 0 44 69 515 661.3 0 515 70 143 661.3 0 143 71 353 661.3 0 353 72 21 661.3 0 21 73 27 661.3 0 27 74 24 661.3 0 24 75 35 661.3 0 35 76 32 661.3 0 32 77 199 661.3 0 199 78 115 661.3 0 115 79 107 661.3 0 107 80 49 661.3 0 49 81 39 661.3 0 39 82 34 661.3 0 34 83 47 661.3 0 47 84 52 661.3 0 52 85 48 661.3 0 48 86 32 661.3 0 32 87 36 661.3 0 36 88 41 661.3 0 41 89 44 661.3 0 44 90 51 661.3 0 51 91 48 661.3 0 48 92 31 661.3 0 31 93 69 661.3 0 69 94 58 661.3 0 58 95 31 661.3 0 31 96 40 661.3 0 40 97 79 661.3 0 79 98 87 661.3 0 87 99 221 661.3 0 221 100 39 661.3 0 39 101 77 661.3 0 77 102 30 661.3 0 30 103 22 661.3 0 22 104 28 661.3 0 28 105 28 661.3 0 28 106 23 661.3 0 23 107 53 661.3 0 53 108 47 661.3 0 47 109 59 661.3 0 59 110 50 661.3 0 50 111 68 661.3 0 68 112 66 661.3 0 66 113 60 661.3 0 60 114 253 661.3 0 253 115 2003 661.3 0 2003 116 2451 661.3 0 2451 117 44 661.3 0 44 118 32 661.3 0 32 119 35 661.3 0 35 120 26 661.3 0 26 121 37 661.3 0 37 122 31 661.3 0 31 123 39 661.3 0 39 124 32 661.3 0 32 125 48 661.3 0 48 126 57 661.3 0 57 127 75 661.3 0 75 128 63 661.3 0 63 129 77 661.3 0 77 130 85 661.3 0 85 131 80 661.3 0 80 132 73 661.3 0 73 133 85 661.3 0 85 134 105 661.3 0 105 135 109 661.3 0 109 136 81 661.3 0 81 137 123 661.3 0 123 138 314 661.3 0 314 139 728 661.3 0 728 140 669 661.3 0 669 141 1512 661.3 0 1512 142 473 661.3 0 473 143 779 661.3 0 779 144 832 661.3 0 832 145 833 661.3 0 833 146 54 661.3 0 54 147 60 661.3 0 60 148 464 661.3 0 464 149 303 661.3 0 303 150 367 661.3 0 367 151 825 661.3 0 825 152 329 661.3 0 329 153 46 661.3 0 46 154 109 661.3 0 109 155 51 661.3 0 51 156 75 661.3 0 75 157 56 661.3 0 56 158 49 661.3 0 49 159 79 661.3 0 79 160 42 661.3 0 42 161 50 661.3 0 50 162 45 661.3 0 45 163 41 661.3 0 41 164 63 661.3 0 63 165 44 661.3 0 44 166 41 661.3 0 41 167 30 661.3 0 30 168 47 661.3 0 47 169 45 661.3 0 45 170 44 661.3 0 44 171 33 661.3 0 33 172 39 661.3 0 39 173 31 661.3 0 31 174 48 661.3 0 48 175 31 661.3 0 31 176 66 661.3 0 66 177 49 661.3 0 49 178 45 661.3 0 45 179 40 661.3 0 40 180 56 661.3 0 56 181 36 661.3 0 36 182 48 661.3 0 48 183 62 661.3 0 62 184 81 661.3 0 81 185 73 661.3 0 73 186 58 661.3 0 58 187 39 661.3 0 39 188 53 661.3 0 53 189 113 661.3 0 113 190 102 661.3 0 102 191 158 661.3 0 158 192 74 661.3 0 74 193 58 661.3 0 58 194 44 661.3 0 44 195 63 661.3 0 63 196 87 661.3 0 87 197 66 661.3 0 66 198 31 661.3 0 31 199 28 661.3 0 28 200 46 661.3 0 46 201 75 661.3 0 75 202 49 661.3 0 49 203 43 661.3 0 43 204 62 661.3 0 62 205 63 661.3 0 63 206 67 661.3 0 67 207 52 661.3 0 52 208 58 661.3 0 58 209 102 661.3 0 102 210 115 661.3 0 115 211 155 661.3 0 155 212 83 661.3 0 83 213 82 661.3 0 82 214 91 661.3 0 91 215 96 661.3 0 96 216 71 661.3 0 71 217 76 661.3 0 76 218 56 661.3 0 56 219 53 661.3 0 53 220 58 661.3 0 58 221 91 661.3 0 91 222 94 661.3 0 94 223 83 661.3 0 83 224 63 661.3 0 63 225 87 661.3 0 87 226 55 661.3 0 55 227 64 661.3 0 64 228 46 661.3 0 46 229 39 661.3 0 39 230 47 661.3 0 47 231 62 661.3 0 62 232 72 661.3 0 72 233 104 661.3 0 104 234 103 661.3 0 103 235 138 661.3 0 138 236 98 661.3 0 98 237 77 661.3 0 77 238 98 661.3 0 98 239 47 661.3 0 47 240 74 661.3 0 74 241 94 661.3 0 94 242 83 661.3 0 83 243 64 661.3 0 64 244 90 661.3 0 90 245 83 661.3 0 83 246 68 661.3 0 68 247 36 661.3 0 36 248 64 661.3 0 64 249 200 661.3 0 200 250 231 661.3 0 231 251 428 661.3 0 428 252 54 661.3 0 54 253 167 661.3 0 167 254 261 661.3 0 261 255 428 661.3 0 428 256 55 661.3 0 55 257 37 661.3 0 37 258 35 661.3 0 35 259 56 661.3 0 56 260 60 661.3 0 60 261 54 661.3 0 54 262 88 661.3 0 88 263 39 661.3 0 39 264 48 661.3 0 48 265 57 661.3 0 57 266 64 661.3 0 64 267 47 661.3 0 47 268 67 661.3 0 67 269 668 661.3 0 668 270 965 661.3 0 965 271 114 661.3 0 114 272 72 661.3 0 72 273 112 661.3 0 112 274 97 661.3 0 97 275 170 661.3 0 170 276 83 661.3 0 83 277 66 661.3 0 66 278 47 661.3 0 47 279 20 661.3 0 20 280 49 661.3 0 49 281 76 661.3 0 76 282 96 661.3 0 96 283 82 661.3 0 82 284 57 661.3 0 57 285 59 661.3 0 59 286 43 661.3 0 43 287 51 661.3 0 51 288 52 661.3 0 52 289 108 661.3 0 108 290 84 661.3 0 84 291 68 661.3 0 68 292 64 661.3 0 64 293 54 661.3 0 54 294 64 661.3 0 64 295 70 661.3 0 70 296 92 661.3 0 92 297 82 661.3 0 82 298 110 661.3 0 110 299 82 661.3 0 82 300 108 661.3 0 108 301 58 661.3 0 58 Finished in 4.20 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 93,493 Reads with adapters: 26,230 (28.1%) Reads that were too short: 103 (0.1%) Reads written (passing filters): 26,127 (27.9%) Total basepairs processed: 24,761,015 bp Total written (filtered): 7,160,301 bp (28.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 26230 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 9 0.0 3 0 0 8 1 16 262 0.0 3 0 243 13 6 17 25526 0.0 3 24894 550 62 20 18 91 0.0 3 9 55 14 13 19 25 0.0 3 0 0 9 16 20 40 0.0 3 0 0 0 40 21 1 0.0 3 0 1 34 83 0.0 3 0 0 0 83 37 3 0.0 3 0 0 0 3 42 1 0.0 3 0 0 0 1 43 2 0.0 3 0 0 0 2 46 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 54 2 0.0 3 0 0 0 2 57 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 62 2 0.0 3 0 0 0 2 69 2 0.0 3 0 0 0 2 77 24 0.0 3 0 0 0 24 80 4 0.0 3 0 0 0 4 85 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 1 97 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 1 135 2 0.0 3 0 0 0 2 138 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 144 3 0.0 3 0 0 1 2 145 1 0.0 3 0 0 0 1 146 44 0.0 3 0 0 1 43 147 1 0.0 3 0 0 0 1 149 10 0.0 3 0 0 0 10 156 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 162 20 0.0 3 0 0 0 20 163 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 178 5 0.0 3 0 0 0 5 179 4 0.0 3 0 0 1 3 183 2 0.0 3 0 0 0 2 184 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 192 7 0.0 3 0 0 0 7 198 1 0.0 3 0 0 0 1 204 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 219 1 0.0 3 0 0 0 1 220 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 271 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 4 0.0 3 0 0 0 4 282 9 0.0 3 0 0 0 9 283 1 0.0 3 0 0 0 1 285 2 0.0 3 0 0 0 2 287 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 289 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.92 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 26,127 Reads with adapters: 1,791 (6.9%) Reads written (passing filters): 26,127 (100.0%) Total basepairs processed: 7,160,301 bp Total written (filtered): 7,154,907 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1791 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 62.7% C: 5.4% G: 28.2% T: 3.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1770 408.2 0 1770 4 21 102.1 0 21