This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCAAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 70.65 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 334,782 (12.4%) Reads that were too short: 17,770 (0.7%) Reads written (passing filters): 317,012 (11.7%) Total basepairs processed: 815,312,680 bp Total written (filtered): 86,018,990 bp (10.6%) === Adapter 1 === Sequence: GTCAAT; Type: regular 5'; Length: 6; Trimmed: 334782 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2050 661.3 0 2050 7 26238 661.3 0 26238 8 3604 661.3 0 3604 9 180541 661.3 0 180541 10 331 661.3 0 331 11 70 661.3 0 70 12 98 661.3 0 98 13 82 661.3 0 82 14 78 661.3 0 78 15 75 661.3 0 75 16 104 661.3 0 104 17 82 661.3 0 82 18 85 661.3 0 85 19 70 661.3 0 70 20 97 661.3 0 97 21 88 661.3 0 88 22 81 661.3 0 81 23 102 661.3 0 102 24 95 661.3 0 95 25 112 661.3 0 112 26 129 661.3 0 129 27 109 661.3 0 109 28 156 661.3 0 156 29 247 661.3 0 247 30 153 661.3 0 153 31 132 661.3 0 132 32 202 661.3 0 202 33 284 661.3 0 284 34 137 661.3 0 137 35 166 661.3 0 166 36 201 661.3 0 201 37 176 661.3 0 176 38 214 661.3 0 214 39 176 661.3 0 176 40 209 661.3 0 209 41 242 661.3 0 242 42 4148 661.3 0 4148 43 4902 661.3 0 4902 44 4490 661.3 0 4490 45 112 661.3 0 112 46 99 661.3 0 99 47 139 661.3 0 139 48 285 661.3 0 285 49 110 661.3 0 110 50 140 661.3 0 140 51 95 661.3 0 95 52 150 661.3 0 150 53 229 661.3 0 229 54 140 661.3 0 140 55 128 661.3 0 128 56 83 661.3 0 83 57 105 661.3 0 105 58 128 661.3 0 128 59 207 661.3 0 207 60 253 661.3 0 253 61 392 661.3 0 392 62 537 661.3 0 537 63 959 661.3 0 959 64 2234 661.3 0 2234 65 28651 661.3 0 28651 66 10465 661.3 0 10465 67 24765 661.3 0 24765 68 172 661.3 0 172 69 104 661.3 0 104 70 60 661.3 0 60 71 57 661.3 0 57 72 79 661.3 0 79 73 86 661.3 0 86 74 68 661.3 0 68 75 75 661.3 0 75 76 68 661.3 0 68 77 102 661.3 0 102 78 77 661.3 0 77 79 80 661.3 0 80 80 82 661.3 0 82 81 86 661.3 0 86 82 99 661.3 0 99 83 87 661.3 0 87 84 70 661.3 0 70 85 74 661.3 0 74 86 71 661.3 0 71 87 118 661.3 0 118 88 93 661.3 0 93 89 96 661.3 0 96 90 86 661.3 0 86 91 70 661.3 0 70 92 71 661.3 0 71 93 113 661.3 0 113 94 120 661.3 0 120 95 115 661.3 0 115 96 89 661.3 0 89 97 189 661.3 0 189 98 118 661.3 0 118 99 95 661.3 0 95 100 143 661.3 0 143 101 115 661.3 0 115 102 212 661.3 0 212 103 115 661.3 0 115 104 123 661.3 0 123 105 114 661.3 0 114 106 102 661.3 0 102 107 98 661.3 0 98 108 76 661.3 0 76 109 72 661.3 0 72 110 99 661.3 0 99 111 134 661.3 0 134 112 104 661.3 0 104 113 118 661.3 0 118 114 94 661.3 0 94 115 97 661.3 0 97 116 75 661.3 0 75 117 110 661.3 0 110 118 101 661.3 0 101 119 127 661.3 0 127 120 111 661.3 0 111 121 112 661.3 0 112 122 81 661.3 0 81 123 94 661.3 0 94 124 102 661.3 0 102 125 91 661.3 0 91 126 126 661.3 0 126 127 135 661.3 0 135 128 123 661.3 0 123 129 108 661.3 0 108 130 87 661.3 0 87 131 98 661.3 0 98 132 119 661.3 0 119 133 199 661.3 0 199 134 183 661.3 0 183 135 119 661.3 0 119 136 162 661.3 0 162 137 163 661.3 0 163 138 292 661.3 0 292 139 378 661.3 0 378 140 169 661.3 0 169 141 146 661.3 0 146 142 106 661.3 0 106 143 73 661.3 0 73 144 131 661.3 0 131 145 110 661.3 0 110 146 105 661.3 0 105 147 117 661.3 0 117 148 115 661.3 0 115 149 109 661.3 0 109 150 121 661.3 0 121 151 106 661.3 0 106 152 84 661.3 0 84 153 82 661.3 0 82 154 100 661.3 0 100 155 99 661.3 0 99 156 98 661.3 0 98 157 138 661.3 0 138 158 114 661.3 0 114 159 177 661.3 0 177 160 134 661.3 0 134 161 144 661.3 0 144 162 93 661.3 0 93 163 147 661.3 0 147 164 116 661.3 0 116 165 118 661.3 0 118 166 131 661.3 0 131 167 93 661.3 0 93 168 155 661.3 0 155 169 126 661.3 0 126 170 105 661.3 0 105 171 113 661.3 0 113 172 157 661.3 0 157 173 177 661.3 0 177 174 145 661.3 0 145 175 88 661.3 0 88 176 107 661.3 0 107 177 104 661.3 0 104 178 133 661.3 0 133 179 149 661.3 0 149 180 127 661.3 0 127 181 126 661.3 0 126 182 121 661.3 0 121 183 121 661.3 0 121 184 185 661.3 0 185 185 254 661.3 0 254 186 177 661.3 0 177 187 151 661.3 0 151 188 118 661.3 0 118 189 157 661.3 0 157 190 137 661.3 0 137 191 134 661.3 0 134 192 96 661.3 0 96 193 108 661.3 0 108 194 136 661.3 0 136 195 128 661.3 0 128 196 125 661.3 0 125 197 124 661.3 0 124 198 83 661.3 0 83 199 158 661.3 0 158 200 206 661.3 0 206 201 161 661.3 0 161 202 86 661.3 0 86 203 120 661.3 0 120 204 127 661.3 0 127 205 118 661.3 0 118 206 157 661.3 0 157 207 187 661.3 0 187 208 169 661.3 0 169 209 176 661.3 0 176 210 134 661.3 0 134 211 115 661.3 0 115 212 126 661.3 0 126 213 119 661.3 0 119 214 132 661.3 0 132 215 177 661.3 0 177 216 177 661.3 0 177 217 242 661.3 0 242 218 155 661.3 0 155 219 188 661.3 0 188 220 234 661.3 0 234 221 165 661.3 0 165 222 192 661.3 0 192 223 149 661.3 0 149 224 216 661.3 0 216 225 184 661.3 0 184 226 170 661.3 0 170 227 163 661.3 0 163 228 163 661.3 0 163 229 214 661.3 0 214 230 178 661.3 0 178 231 139 661.3 0 139 232 117 661.3 0 117 233 121 661.3 0 121 234 135 661.3 0 135 235 142 661.3 0 142 236 195 661.3 0 195 237 170 661.3 0 170 238 155 661.3 0 155 239 148 661.3 0 148 240 207 661.3 0 207 241 188 661.3 0 188 242 217 661.3 0 217 243 219 661.3 0 219 244 238 661.3 0 238 245 217 661.3 0 217 246 157 661.3 0 157 247 174 661.3 0 174 248 203 661.3 0 203 249 284 661.3 0 284 250 129 661.3 0 129 251 139 661.3 0 139 252 84 661.3 0 84 253 148 661.3 0 148 254 205 661.3 0 205 255 388 661.3 0 388 256 505 661.3 0 505 257 477 661.3 0 477 258 234 661.3 0 234 259 230 661.3 0 230 260 271 661.3 0 271 261 450 661.3 0 450 262 274 661.3 0 274 263 146 661.3 0 146 264 152 661.3 0 152 265 217 661.3 0 217 266 234 661.3 0 234 267 188 661.3 0 188 268 182 661.3 0 182 269 237 661.3 0 237 270 159 661.3 0 159 271 116 661.3 0 116 272 184 661.3 0 184 273 100 661.3 0 100 274 159 661.3 0 159 275 185 661.3 0 185 276 212 661.3 0 212 277 142 661.3 0 142 278 97 661.3 0 97 279 118 661.3 0 118 280 197 661.3 0 197 281 201 661.3 0 201 282 124 661.3 0 124 283 78 661.3 0 78 284 114 661.3 0 114 285 113 661.3 0 113 286 111 661.3 0 111 287 156 661.3 0 156 288 285 661.3 0 285 289 215 661.3 0 215 290 93 661.3 0 93 291 78 661.3 0 78 292 161 661.3 0 161 293 155 661.3 0 155 294 309 661.3 0 309 295 213 661.3 0 213 296 158 661.3 0 158 297 96 661.3 0 96 298 78 661.3 0 78 299 72 661.3 0 72 300 132 661.3 0 132 301 110 661.3 0 110 Finished in 15.44 s (49 us/read; 1.23 M reads/minute). === Summary === Total reads processed: 317,012 Reads with adapters: 26,566 (8.4%) Reads that were too short: 19 (0.0%) Reads written (passing filters): 26,547 (8.4%) Total basepairs processed: 86,018,990 bp Total written (filtered): 7,192,469 bp (8.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 26566 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 3 0.0 3 0 0 0 2 1 18 10 0.0 3 0 0 9 1 19 287 0.0 3 0 275 10 1 1 20 25209 0.0 4 24898 299 8 3 1 21 49 0.0 4 9 40 22 13 0.0 4 2 3 7 0 1 23 224 0.0 4 217 3 1 2 1 24 2 0.0 4 0 0 0 0 2 26 1 0.0 4 0 0 0 1 35 1 0.0 4 0 0 0 0 1 37 1 0.0 4 1 51 1 0.0 4 0 0 0 0 1 63 2 0.0 4 0 0 0 0 2 67 1 0.0 4 0 0 0 1 69 1 0.0 4 0 0 0 0 1 92 2 0.0 4 0 0 0 0 2 98 1 0.0 4 0 0 0 0 1 102 1 0.0 4 0 0 0 0 1 112 2 0.0 4 0 0 0 0 2 113 2 0.0 4 0 0 0 0 2 114 1 0.0 4 0 0 0 0 1 115 1 0.0 4 0 0 0 0 1 119 1 0.0 4 0 0 0 0 1 125 4 0.0 4 0 0 0 0 4 126 13 0.0 4 0 0 0 0 13 127 706 0.0 4 0 0 0 0 706 128 2 0.0 4 0 0 0 0 2 133 1 0.0 4 1 135 1 0.0 4 1 138 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 153 2 0.0 4 0 0 0 0 2 167 1 0.0 4 0 0 0 0 1 182 1 0.0 4 1 193 2 0.0 4 0 0 0 0 2 203 1 0.0 4 0 0 0 0 1 212 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 230 1 0.0 4 0 0 0 0 1 232 1 0.0 4 0 0 0 0 1 235 3 0.0 4 0 0 0 0 3 255 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 260 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.87 s (33 us/read; 1.83 M reads/minute). === Summary === Total reads processed: 26,547 Reads with adapters: 273 (1.0%) Reads written (passing filters): 26,547 (100.0%) Total basepairs processed: 7,192,469 bp Total written (filtered): 7,187,526 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 273 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 4.4% C: 2.2% G: 89.7% T: 3.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 6 414.8 0 6 4 4 103.7 0 4 5 1 25.9 0 1 9 3 0.1 0 0 3 10 5 0.0 1 1 4 11 235 0.0 1 2 233 12 7 0.0 1 0 7 111 1 0.0 1 1 113 1 0.0 1 1 152 4 0.0 1 0 4 158 1 0.0 1 1 210 2 0.0 1 1 1 225 1 0.0 1 1 259 1 0.0 1 0 1 264 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.