This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTATGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 70.48 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 254,342 (9.4%) Reads that were too short: 13,524 (0.5%) Reads written (passing filters): 240,818 (8.9%) Total basepairs processed: 815,312,680 bp Total written (filtered): 68,205,935 bp (8.4%) === Adapter 1 === Sequence: GTATGT; Type: regular 5'; Length: 6; Trimmed: 254342 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1205 661.3 0 1205 7 1650 661.3 0 1650 8 29451 661.3 0 29451 9 188294 661.3 0 188294 10 328 661.3 0 328 11 62 661.3 0 62 12 52 661.3 0 52 13 43 661.3 0 43 14 31 661.3 0 31 15 37 661.3 0 37 16 28 661.3 0 28 17 46 661.3 0 46 18 39 661.3 0 39 19 51 661.3 0 51 20 42 661.3 0 42 21 28 661.3 0 28 22 37 661.3 0 37 23 30 661.3 0 30 24 50 661.3 0 50 25 48 661.3 0 48 26 28 661.3 0 28 27 31 661.3 0 31 28 35 661.3 0 35 29 42 661.3 0 42 30 38 661.3 0 38 31 35 661.3 0 35 32 25 661.3 0 25 33 32 661.3 0 32 34 36 661.3 0 36 35 39 661.3 0 39 36 33 661.3 0 33 37 31 661.3 0 31 38 40 661.3 0 40 39 44 661.3 0 44 40 79 661.3 0 79 41 17 661.3 0 17 42 32 661.3 0 32 43 38 661.3 0 38 44 58 661.3 0 58 45 50 661.3 0 50 46 36 661.3 0 36 47 42 661.3 0 42 48 39 661.3 0 39 49 399 661.3 0 399 50 27 661.3 0 27 51 122 661.3 0 122 52 93 661.3 0 93 53 41 661.3 0 41 54 28 661.3 0 28 55 32 661.3 0 32 56 30 661.3 0 30 57 39 661.3 0 39 58 40 661.3 0 40 59 32 661.3 0 32 60 18 661.3 0 18 61 42 661.3 0 42 62 45 661.3 0 45 63 49 661.3 0 49 64 51 661.3 0 51 65 49 661.3 0 49 66 37 661.3 0 37 67 54 661.3 0 54 68 31 661.3 0 31 69 48 661.3 0 48 70 54 661.3 0 54 71 96 661.3 0 96 72 49 661.3 0 49 73 45 661.3 0 45 74 46 661.3 0 46 75 38 661.3 0 38 76 53 661.3 0 53 77 68 661.3 0 68 78 69 661.3 0 69 79 110 661.3 0 110 80 727 661.3 0 727 81 289 661.3 0 289 82 324 661.3 0 324 83 91 661.3 0 91 84 37 661.3 0 37 85 76 661.3 0 76 86 77 661.3 0 77 87 61 661.3 0 61 88 41 661.3 0 41 89 64 661.3 0 64 90 52 661.3 0 52 91 63 661.3 0 63 92 57 661.3 0 57 93 80 661.3 0 80 94 80 661.3 0 80 95 93 661.3 0 93 96 923 661.3 0 923 97 1207 661.3 0 1207 98 2241 661.3 0 2241 99 85 661.3 0 85 100 50 661.3 0 50 101 45 661.3 0 45 102 48 661.3 0 48 103 38 661.3 0 38 104 38 661.3 0 38 105 39 661.3 0 39 106 48 661.3 0 48 107 40 661.3 0 40 108 60 661.3 0 60 109 86 661.3 0 86 110 43 661.3 0 43 111 67 661.3 0 67 112 44 661.3 0 44 113 65 661.3 0 65 114 83 661.3 0 83 115 51 661.3 0 51 116 46 661.3 0 46 117 49 661.3 0 49 118 68 661.3 0 68 119 59 661.3 0 59 120 57 661.3 0 57 121 53 661.3 0 53 122 47 661.3 0 47 123 51 661.3 0 51 124 58 661.3 0 58 125 45 661.3 0 45 126 43 661.3 0 43 127 70 661.3 0 70 128 143 661.3 0 143 129 87 661.3 0 87 130 158 661.3 0 158 131 78 661.3 0 78 132 321 661.3 0 321 133 113 661.3 0 113 134 194 661.3 0 194 135 79 661.3 0 79 136 63 661.3 0 63 137 69 661.3 0 69 138 112 661.3 0 112 139 69 661.3 0 69 140 68 661.3 0 68 141 69 661.3 0 69 142 57 661.3 0 57 143 65 661.3 0 65 144 66 661.3 0 66 145 56 661.3 0 56 146 60 661.3 0 60 147 60 661.3 0 60 148 226 661.3 0 226 149 705 661.3 0 705 150 244 661.3 0 244 151 41 661.3 0 41 152 40 661.3 0 40 153 56 661.3 0 56 154 139 661.3 0 139 155 58 661.3 0 58 156 49 661.3 0 49 157 64 661.3 0 64 158 71 661.3 0 71 159 40 661.3 0 40 160 50 661.3 0 50 161 56 661.3 0 56 162 52 661.3 0 52 163 47 661.3 0 47 164 60 661.3 0 60 165 57 661.3 0 57 166 58 661.3 0 58 167 60 661.3 0 60 168 41 661.3 0 41 169 54 661.3 0 54 170 71 661.3 0 71 171 54 661.3 0 54 172 62 661.3 0 62 173 53 661.3 0 53 174 57 661.3 0 57 175 62 661.3 0 62 176 73 661.3 0 73 177 60 661.3 0 60 178 50 661.3 0 50 179 66 661.3 0 66 180 75 661.3 0 75 181 80 661.3 0 80 182 65 661.3 0 65 183 57 661.3 0 57 184 101 661.3 0 101 185 107 661.3 0 107 186 95 661.3 0 95 187 272 661.3 0 272 188 458 661.3 0 458 189 827 661.3 0 827 190 556 661.3 0 556 191 98 661.3 0 98 192 63 661.3 0 63 193 61 661.3 0 61 194 65 661.3 0 65 195 57 661.3 0 57 196 100 661.3 0 100 197 57 661.3 0 57 198 54 661.3 0 54 199 44 661.3 0 44 200 53 661.3 0 53 201 84 661.3 0 84 202 60 661.3 0 60 203 71 661.3 0 71 204 70 661.3 0 70 205 83 661.3 0 83 206 89 661.3 0 89 207 93 661.3 0 93 208 98 661.3 0 98 209 285 661.3 0 285 210 313 661.3 0 313 211 356 661.3 0 356 212 178 661.3 0 178 213 82 661.3 0 82 214 73 661.3 0 73 215 88 661.3 0 88 216 59 661.3 0 59 217 70 661.3 0 70 218 61 661.3 0 61 219 95 661.3 0 95 220 80 661.3 0 80 221 89 661.3 0 89 222 102 661.3 0 102 223 90 661.3 0 90 224 88 661.3 0 88 225 108 661.3 0 108 226 131 661.3 0 131 227 97 661.3 0 97 228 122 661.3 0 122 229 80 661.3 0 80 230 108 661.3 0 108 231 89 661.3 0 89 232 138 661.3 0 138 233 127 661.3 0 127 234 198 661.3 0 198 235 173 661.3 0 173 236 156 661.3 0 156 237 147 661.3 0 147 238 148 661.3 0 148 239 99 661.3 0 99 240 211 661.3 0 211 241 404 661.3 0 404 242 674 661.3 0 674 243 160 661.3 0 160 244 154 661.3 0 154 245 112 661.3 0 112 246 82 661.3 0 82 247 86 661.3 0 86 248 83 661.3 0 83 249 257 661.3 0 257 250 445 661.3 0 445 251 832 661.3 0 832 252 95 661.3 0 95 253 281 661.3 0 281 254 510 661.3 0 510 255 298 661.3 0 298 256 152 661.3 0 152 257 112 661.3 0 112 258 77 661.3 0 77 259 71 661.3 0 71 260 165 661.3 0 165 261 215 661.3 0 215 262 192 661.3 0 192 263 83 661.3 0 83 264 81 661.3 0 81 265 84 661.3 0 84 266 81 661.3 0 81 267 36 661.3 0 36 268 120 661.3 0 120 269 128 661.3 0 128 270 175 661.3 0 175 271 178 661.3 0 178 272 114 661.3 0 114 273 87 661.3 0 87 274 123 661.3 0 123 275 93 661.3 0 93 276 65 661.3 0 65 277 84 661.3 0 84 278 78 661.3 0 78 279 61 661.3 0 61 280 65 661.3 0 65 281 100 661.3 0 100 282 133 661.3 0 133 283 77 661.3 0 77 284 93 661.3 0 93 285 92 661.3 0 92 286 59 661.3 0 59 287 38 661.3 0 38 288 69 661.3 0 69 289 87 661.3 0 87 290 73 661.3 0 73 291 47 661.3 0 47 292 68 661.3 0 68 293 52 661.3 0 52 294 48 661.3 0 48 295 52 661.3 0 52 296 56 661.3 0 56 297 55 661.3 0 55 298 70 661.3 0 70 299 66 661.3 0 66 300 81 661.3 0 81 301 110 661.3 0 110 Finished in 10.51 s (44 us/read; 1.37 M reads/minute). === Summary === Total reads processed: 240,818 Reads with adapters: 195,690 (81.3%) Reads that were too short: 9 (0.0%) Reads written (passing filters): 195,681 (81.3%) Total basepairs processed: 68,205,935 bp Total written (filtered): 53,235,890 bp (78.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 195690 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 11 0.0 3 0 0 0 4 7 18 87 0.0 3 0 0 86 0 1 19 2670 0.0 3 0 2619 40 6 5 20 192380 0.0 4 189917 2363 78 17 5 21 389 0.0 4 61 320 8 22 31 0.0 4 1 1 27 1 1 23 84 0.0 4 1 0 0 75 8 24 8 0.0 4 5 0 0 1 2 25 4 0.0 4 3 0 0 1 27 1 0.0 4 1 28 3 0.0 4 2 0 0 1 29 1 0.0 4 1 37 1 0.0 4 1 38 1 0.0 4 0 0 0 0 1 52 1 0.0 4 0 0 0 0 1 58 2 0.0 4 0 0 0 0 2 76 1 0.0 4 0 0 0 0 1 87 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 2 0.0 4 0 0 0 0 2 130 4 0.0 4 0 0 0 0 4 152 1 0.0 4 0 0 0 0 1 181 1 0.0 4 0 0 0 0 1 192 1 0.0 4 0 0 0 0 1 193 1 0.0 4 0 0 0 0 1 284 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 6.62 s (34 us/read; 1.77 M reads/minute). === Summary === Total reads processed: 195,681 Reads with adapters: 5,234 (2.7%) Reads written (passing filters): 195,681 (100.0%) Total basepairs processed: 53,235,890 bp Total written (filtered): 53,176,876 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 5234 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.0% C: 1.9% G: 94.9% T: 1.1% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 9 3057.5 0 9 4 32 764.4 0 32 5 5 191.1 0 5 6 5 47.8 0 5 8 1 3.0 0 1 9 445 0.7 0 29 416 10 3085 0.2 1 105 2980 11 1607 0.0 1 0 1607 12 6 0.0 1 0 6 13 3 0.0 1 0 3 22 4 0.0 1 3 1 125 1 0.0 1 0 1 150 6 0.0 1 0 6 156 3 0.0 1 3 169 1 0.0 1 1 177 1 0.0 1 0 1 208 18 0.0 1 13 5 210 1 0.0 1 1 272 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.