This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACAACC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 70.81 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 305,190 (11.3%) Reads that were too short: 142,765 (5.3%) Reads written (passing filters): 162,425 (6.0%) Total basepairs processed: 815,312,680 bp Total written (filtered): 35,644,304 bp (4.4%) === Adapter 1 === Sequence: ACAACC; Type: regular 5'; Length: 6; Trimmed: 305190 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 573 661.3 0 573 7 12612 661.3 0 12612 8 8876 661.3 0 8876 9 620 661.3 0 620 10 1321 661.3 0 1321 11 50470 661.3 0 50470 12 369 661.3 0 369 13 48 661.3 0 48 14 48 661.3 0 48 15 77 661.3 0 77 16 44 661.3 0 44 17 61 661.3 0 61 18 51 661.3 0 51 19 43 661.3 0 43 20 51 661.3 0 51 21 43 661.3 0 43 22 44 661.3 0 44 23 42 661.3 0 42 24 64 661.3 0 64 25 64 661.3 0 64 26 37 661.3 0 37 27 46 661.3 0 46 28 30 661.3 0 30 29 47 661.3 0 47 30 43 661.3 0 43 31 54 661.3 0 54 32 60 661.3 0 60 33 49 661.3 0 49 34 46 661.3 0 46 35 66 661.3 0 66 36 51 661.3 0 51 37 48 661.3 0 48 38 50 661.3 0 50 39 48 661.3 0 48 40 39 661.3 0 39 41 52 661.3 0 52 42 36 661.3 0 36 43 46 661.3 0 46 44 54 661.3 0 54 45 52 661.3 0 52 46 34 661.3 0 34 47 36 661.3 0 36 48 49 661.3 0 49 49 37 661.3 0 37 50 44 661.3 0 44 51 45 661.3 0 45 52 55 661.3 0 55 53 79 661.3 0 79 54 82 661.3 0 82 55 44 661.3 0 44 56 58 661.3 0 58 57 48 661.3 0 48 58 52 661.3 0 52 59 76 661.3 0 76 60 68 661.3 0 68 61 73 661.3 0 73 62 60 661.3 0 60 63 69 661.3 0 69 64 75 661.3 0 75 65 69 661.3 0 69 66 57 661.3 0 57 67 55 661.3 0 55 68 111 661.3 0 111 69 87 661.3 0 87 70 110 661.3 0 110 71 177 661.3 0 177 72 1013 661.3 0 1013 73 635 661.3 0 635 74 696 661.3 0 696 75 528 661.3 0 528 76 200 661.3 0 200 77 145 661.3 0 145 78 297 661.3 0 297 79 294 661.3 0 294 80 347 661.3 0 347 81 379 661.3 0 379 82 394 661.3 0 394 83 319 661.3 0 319 84 693 661.3 0 693 85 478 661.3 0 478 86 465 661.3 0 465 87 473 661.3 0 473 88 581 661.3 0 581 89 220 661.3 0 220 90 528 661.3 0 528 91 631 661.3 0 631 92 442 661.3 0 442 93 518 661.3 0 518 94 250 661.3 0 250 95 253 661.3 0 253 96 169 661.3 0 169 97 114 661.3 0 114 98 229 661.3 0 229 99 236 661.3 0 236 100 172 661.3 0 172 101 420 661.3 0 420 102 335 661.3 0 335 103 291 661.3 0 291 104 427 661.3 0 427 105 685 661.3 0 685 106 661 661.3 0 661 107 427 661.3 0 427 108 466 661.3 0 466 109 418 661.3 0 418 110 356 661.3 0 356 111 428 661.3 0 428 112 324 661.3 0 324 113 347 661.3 0 347 114 313 661.3 0 313 115 415 661.3 0 415 116 481 661.3 0 481 117 467 661.3 0 467 118 452 661.3 0 452 119 463 661.3 0 463 120 468 661.3 0 468 121 609 661.3 0 609 122 768 661.3 0 768 123 651 661.3 0 651 124 802 661.3 0 802 125 953 661.3 0 953 126 729 661.3 0 729 127 383 661.3 0 383 128 372 661.3 0 372 129 461 661.3 0 461 130 704 661.3 0 704 131 637 661.3 0 637 132 824 661.3 0 824 133 828 661.3 0 828 134 801 661.3 0 801 135 646 661.3 0 646 136 526 661.3 0 526 137 666 661.3 0 666 138 596 661.3 0 596 139 495 661.3 0 495 140 327 661.3 0 327 141 586 661.3 0 586 142 679 661.3 0 679 143 700 661.3 0 700 144 636 661.3 0 636 145 771 661.3 0 771 146 835 661.3 0 835 147 737 661.3 0 737 148 1065 661.3 0 1065 149 876 661.3 0 876 150 984 661.3 0 984 151 817 661.3 0 817 152 688 661.3 0 688 153 747 661.3 0 747 154 711 661.3 0 711 155 821 661.3 0 821 156 809 661.3 0 809 157 797 661.3 0 797 158 912 661.3 0 912 159 757 661.3 0 757 160 688 661.3 0 688 161 758 661.3 0 758 162 1026 661.3 0 1026 163 946 661.3 0 946 164 742 661.3 0 742 165 684 661.3 0 684 166 701 661.3 0 701 167 846 661.3 0 846 168 897 661.3 0 897 169 1176 661.3 0 1176 170 1516 661.3 0 1516 171 1638 661.3 0 1638 172 1076 661.3 0 1076 173 856 661.3 0 856 174 788 661.3 0 788 175 763 661.3 0 763 176 733 661.3 0 733 177 609 661.3 0 609 178 930 661.3 0 930 179 901 661.3 0 901 180 758 661.3 0 758 181 633 661.3 0 633 182 755 661.3 0 755 183 897 661.3 0 897 184 666 661.3 0 666 185 566 661.3 0 566 186 570 661.3 0 570 187 1093 661.3 0 1093 188 1317 661.3 0 1317 189 975 661.3 0 975 190 920 661.3 0 920 191 826 661.3 0 826 192 647 661.3 0 647 193 692 661.3 0 692 194 748 661.3 0 748 195 1218 661.3 0 1218 196 792 661.3 0 792 197 492 661.3 0 492 198 522 661.3 0 522 199 660 661.3 0 660 200 1133 661.3 0 1133 201 997 661.3 0 997 202 550 661.3 0 550 203 822 661.3 0 822 204 606 661.3 0 606 205 671 661.3 0 671 206 427 661.3 0 427 207 496 661.3 0 496 208 403 661.3 0 403 209 538 661.3 0 538 210 472 661.3 0 472 211 492 661.3 0 492 212 582 661.3 0 582 213 789 661.3 0 789 214 1435 661.3 0 1435 215 1604 661.3 0 1604 216 1173 661.3 0 1173 217 1149 661.3 0 1149 218 783 661.3 0 783 219 526 661.3 0 526 220 508 661.3 0 508 221 1033 661.3 0 1033 222 752 661.3 0 752 223 784 661.3 0 784 224 1057 661.3 0 1057 225 1352 661.3 0 1352 226 2869 661.3 0 2869 227 5772 661.3 0 5772 228 2614 661.3 0 2614 229 3342 661.3 0 3342 230 1820 661.3 0 1820 231 2715 661.3 0 2715 232 2424 661.3 0 2424 233 1906 661.3 0 1906 234 676 661.3 0 676 235 450 661.3 0 450 236 582 661.3 0 582 237 1163 661.3 0 1163 238 881 661.3 0 881 239 776 661.3 0 776 240 962 661.3 0 962 241 689 661.3 0 689 242 587 661.3 0 587 243 603 661.3 0 603 244 704 661.3 0 704 245 666 661.3 0 666 246 454 661.3 0 454 247 595 661.3 0 595 248 1234 661.3 0 1234 249 2060 661.3 0 2060 250 2186 661.3 0 2186 251 1092 661.3 0 1092 252 855 661.3 0 855 253 1023 661.3 0 1023 254 794 661.3 0 794 255 694 661.3 0 694 256 547 661.3 0 547 257 623 661.3 0 623 258 621 661.3 0 621 259 872 661.3 0 872 260 944 661.3 0 944 261 1171 661.3 0 1171 262 1128 661.3 0 1128 263 1697 661.3 0 1697 264 3557 661.3 0 3557 265 4425 661.3 0 4425 266 2678 661.3 0 2678 267 1690 661.3 0 1690 268 1814 661.3 0 1814 269 1173 661.3 0 1173 270 1279 661.3 0 1279 271 1329 661.3 0 1329 272 1343 661.3 0 1343 273 1637 661.3 0 1637 274 1365 661.3 0 1365 275 1833 661.3 0 1833 276 2445 661.3 0 2445 277 1533 661.3 0 1533 278 1029 661.3 0 1029 279 784 661.3 0 784 280 1236 661.3 0 1236 281 1842 661.3 0 1842 282 1280 661.3 0 1280 283 937 661.3 0 937 284 1151 661.3 0 1151 285 1094 661.3 0 1094 286 795 661.3 0 795 287 921 661.3 0 921 288 1079 661.3 0 1079 289 1104 661.3 0 1104 290 1529 661.3 0 1529 291 4486 661.3 0 4486 292 10337 661.3 0 10337 293 9429 661.3 0 9429 294 623 661.3 0 623 295 439 661.3 0 439 296 597 661.3 0 597 297 773 661.3 0 773 298 899 661.3 0 899 299 860 661.3 0 860 300 726 661.3 0 726 301 919 661.3 0 919 Finished in 6.13 s (38 us/read; 1.59 M reads/minute). === Summary === Total reads processed: 162,425 Reads with adapters: 12,415 (7.6%) Reads that were too short: 183 (0.1%) Reads written (passing filters): 12,232 (7.5%) Total basepairs processed: 35,644,304 bp Total written (filtered): 3,335,058 bp (9.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 12415 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 3 0.0 3 0 0 2 1 16 112 0.0 3 0 103 7 2 17 11721 0.0 3 11461 221 35 4 18 33 0.0 3 6 21 4 2 19 7 0.0 3 1 0 5 1 20 11 0.0 3 1 0 0 10 22 2 0.0 3 0 0 0 2 24 1 0.0 3 0 0 0 1 25 2 0.0 3 0 0 1 1 28 1 0.0 3 0 0 0 1 29 5 0.0 3 2 1 0 2 31 1 0.0 3 1 33 5 0.0 3 0 0 0 5 34 1 0.0 3 1 35 1 0.0 3 0 0 0 1 36 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 41 2 0.0 3 1 0 0 1 42 2 0.0 3 0 0 0 2 44 2 0.0 3 0 0 0 2 45 1 0.0 3 0 0 0 1 47 2 0.0 3 0 0 0 2 49 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 66 2 0.0 3 0 0 0 2 67 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 69 2 0.0 3 0 0 0 2 70 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 81 2 0.0 3 0 0 0 2 86 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 91 3 0.0 3 0 0 0 3 92 4 0.0 3 0 0 0 4 93 1 0.0 3 0 0 0 1 94 2 0.0 3 0 0 1 1 95 2 0.0 3 0 0 0 2 98 2 0.0 3 0 0 0 2 102 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 113 7 0.0 3 0 0 0 7 119 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 1 145 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 157 9 0.0 3 0 0 0 9 158 3 0.0 3 0 0 0 3 160 3 0.0 3 0 0 1 2 161 1 0.0 3 0 0 0 1 162 79 0.0 3 0 0 0 79 163 12 0.0 3 0 0 0 12 164 28 0.0 3 0 0 0 28 165 15 0.0 3 0 0 0 15 166 3 0.0 3 0 0 0 3 167 3 0.0 3 0 0 0 3 168 5 0.0 3 0 0 0 5 171 1 0.0 3 0 0 0 1 175 2 0.0 3 0 0 0 2 176 1 0.0 3 0 0 0 1 177 4 0.0 3 0 0 0 4 178 1 0.0 3 1 179 4 0.0 3 0 0 0 4 180 1 0.0 3 0 0 0 1 181 2 0.0 3 0 0 0 2 182 90 0.0 3 0 0 1 89 183 13 0.0 3 0 0 0 13 184 1 0.0 3 0 0 0 1 185 8 0.0 3 0 0 1 7 186 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 1 188 1 0.0 3 0 0 0 1 197 1 0.0 3 0 0 0 1 201 2 0.0 3 0 0 0 2 205 1 0.0 3 0 0 0 1 213 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 229 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 249 1 0.0 3 0 0 0 1 253 1 0.0 3 0 0 0 1 260 1 0.0 3 0 0 0 1 263 2 0.0 3 0 0 0 2 265 2 0.0 3 0 0 0 2 272 1 0.0 3 0 0 0 1 273 1 0.0 3 0 0 0 1 274 60 0.0 3 0 0 3 57 275 2 0.0 3 0 0 0 2 276 4 0.0 3 0 0 0 4 277 27 0.0 3 0 0 1 26 278 7 0.0 3 0 0 0 7 279 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 4 0.0 3 0 0 0 4 282 2 0.0 3 0 0 0 2 283 11 0.0 3 0 0 0 11 284 12 0.0 3 0 0 0 12 285 2 0.0 3 0 0 0 2 287 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.42 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 12,232 Reads with adapters: 642 (5.2%) Reads written (passing filters): 12,232 (100.0%) Total basepairs processed: 3,335,058 bp Total written (filtered): 3,333,121 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 642 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 34.4% C: 50.5% G: 7.5% T: 7.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 633 191.1 0 633 4 7 47.8 0 7 5 2 11.9 0 2