This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTTGCC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 70.23 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 32,151 (1.2%) Reads that were too short: 3,611 (0.1%) Reads written (passing filters): 28,540 (1.1%) Total basepairs processed: 815,312,680 bp Total written (filtered): 7,528,409 bp (0.9%) === Adapter 1 === Sequence: GTTGCC; Type: regular 5'; Length: 6; Trimmed: 32151 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 331 661.3 0 331 7 7037 661.3 0 7037 8 11857 661.3 0 11857 9 49 661.3 0 49 10 28 661.3 0 28 11 39 661.3 0 39 12 38 661.3 0 38 13 30 661.3 0 30 14 40 661.3 0 40 15 31 661.3 0 31 16 37 661.3 0 37 17 39 661.3 0 39 18 30 661.3 0 30 19 39 661.3 0 39 20 34 661.3 0 34 21 35 661.3 0 35 22 35 661.3 0 35 23 30 661.3 0 30 24 40 661.3 0 40 25 29 661.3 0 29 26 30 661.3 0 30 27 20 661.3 0 20 28 28 661.3 0 28 29 25 661.3 0 25 30 39 661.3 0 39 31 41 661.3 0 41 32 31 661.3 0 31 33 31 661.3 0 31 34 21 661.3 0 21 35 33 661.3 0 33 36 47 661.3 0 47 37 25 661.3 0 25 38 39 661.3 0 39 39 38 661.3 0 38 40 39 661.3 0 39 41 31 661.3 0 31 42 34 661.3 0 34 43 49 661.3 0 49 44 29 661.3 0 29 45 35 661.3 0 35 46 37 661.3 0 37 47 22 661.3 0 22 48 42 661.3 0 42 49 42 661.3 0 42 50 44 661.3 0 44 51 98 661.3 0 98 52 69 661.3 0 69 53 40 661.3 0 40 54 43 661.3 0 43 55 33 661.3 0 33 56 55 661.3 0 55 57 43 661.3 0 43 58 30 661.3 0 30 59 41 661.3 0 41 60 38 661.3 0 38 61 42 661.3 0 42 62 37 661.3 0 37 63 21 661.3 0 21 64 42 661.3 0 42 65 36 661.3 0 36 66 40 661.3 0 40 67 43 661.3 0 43 68 54 661.3 0 54 69 273 661.3 0 273 70 106 661.3 0 106 71 319 661.3 0 319 72 697 661.3 0 697 73 289 661.3 0 289 74 224 661.3 0 224 75 50 661.3 0 50 76 21 661.3 0 21 77 20 661.3 0 20 78 27 661.3 0 27 79 33 661.3 0 33 80 35 661.3 0 35 81 58 661.3 0 58 82 54 661.3 0 54 83 65 661.3 0 65 84 391 661.3 0 391 85 87 661.3 0 87 86 168 661.3 0 168 87 26 661.3 0 26 88 30 661.3 0 30 89 30 661.3 0 30 90 7 661.3 0 7 91 21 661.3 0 21 92 25 661.3 0 25 93 35 661.3 0 35 94 47 661.3 0 47 95 31 661.3 0 31 96 21 661.3 0 21 97 35 661.3 0 35 98 27 661.3 0 27 99 41 661.3 0 41 100 38 661.3 0 38 101 28 661.3 0 28 102 34 661.3 0 34 103 21 661.3 0 21 104 13 661.3 0 13 105 29 661.3 0 29 106 27 661.3 0 27 107 37 661.3 0 37 108 31 661.3 0 31 109 30 661.3 0 30 110 41 661.3 0 41 111 263 661.3 0 263 112 31 661.3 0 31 113 27 661.3 0 27 114 24 661.3 0 24 115 19 661.3 0 19 116 22 661.3 0 22 117 21 661.3 0 21 118 24 661.3 0 24 119 19 661.3 0 19 120 41 661.3 0 41 121 24 661.3 0 24 122 21 661.3 0 21 123 17 661.3 0 17 124 38 661.3 0 38 125 51 661.3 0 51 126 36 661.3 0 36 127 28 661.3 0 28 128 31 661.3 0 31 129 30 661.3 0 30 130 20 661.3 0 20 131 140 661.3 0 140 132 28 661.3 0 28 133 18 661.3 0 18 134 22 661.3 0 22 135 34 661.3 0 34 136 31 661.3 0 31 137 21 661.3 0 21 138 34 661.3 0 34 139 33 661.3 0 33 140 25 661.3 0 25 141 19 661.3 0 19 142 33 661.3 0 33 143 22 661.3 0 22 144 22 661.3 0 22 145 22 661.3 0 22 146 28 661.3 0 28 147 40 661.3 0 40 148 97 661.3 0 97 149 52 661.3 0 52 150 56 661.3 0 56 151 32 661.3 0 32 152 24 661.3 0 24 153 28 661.3 0 28 154 27 661.3 0 27 155 29 661.3 0 29 156 40 661.3 0 40 157 29 661.3 0 29 158 35 661.3 0 35 159 23 661.3 0 23 160 13 661.3 0 13 161 31 661.3 0 31 162 26 661.3 0 26 163 23 661.3 0 23 164 20 661.3 0 20 165 19 661.3 0 19 166 23 661.3 0 23 167 32 661.3 0 32 168 78 661.3 0 78 169 45 661.3 0 45 170 34 661.3 0 34 171 29 661.3 0 29 172 32 661.3 0 32 173 26 661.3 0 26 174 35 661.3 0 35 175 24 661.3 0 24 176 97 661.3 0 97 177 35 661.3 0 35 178 66 661.3 0 66 179 25 661.3 0 25 180 33 661.3 0 33 181 22 661.3 0 22 182 71 661.3 0 71 183 40 661.3 0 40 184 34 661.3 0 34 185 18 661.3 0 18 186 32 661.3 0 32 187 31 661.3 0 31 188 34 661.3 0 34 189 27 661.3 0 27 190 27 661.3 0 27 191 29 661.3 0 29 192 24 661.3 0 24 193 28 661.3 0 28 194 26 661.3 0 26 195 53 661.3 0 53 196 56 661.3 0 56 197 62 661.3 0 62 198 111 661.3 0 111 199 82 661.3 0 82 200 23 661.3 0 23 201 26 661.3 0 26 202 40 661.3 0 40 203 97 661.3 0 97 204 143 661.3 0 143 205 86 661.3 0 86 206 53 661.3 0 53 207 32 661.3 0 32 208 23 661.3 0 23 209 21 661.3 0 21 210 34 661.3 0 34 211 28 661.3 0 28 212 25 661.3 0 25 213 69 661.3 0 69 214 79 661.3 0 79 215 77 661.3 0 77 216 20 661.3 0 20 217 41 661.3 0 41 218 28 661.3 0 28 219 30 661.3 0 30 220 22 661.3 0 22 221 34 661.3 0 34 222 53 661.3 0 53 223 86 661.3 0 86 224 190 661.3 0 190 225 137 661.3 0 137 226 22 661.3 0 22 227 33 661.3 0 33 228 28 661.3 0 28 229 13 661.3 0 13 230 15 661.3 0 15 231 20 661.3 0 20 232 18 661.3 0 18 233 27 661.3 0 27 234 22 661.3 0 22 235 22 661.3 0 22 236 31 661.3 0 31 237 23 661.3 0 23 238 29 661.3 0 29 239 39 661.3 0 39 240 32 661.3 0 32 241 30 661.3 0 30 242 33 661.3 0 33 243 17 661.3 0 17 244 41 661.3 0 41 245 26 661.3 0 26 246 29 661.3 0 29 247 28 661.3 0 28 248 28 661.3 0 28 249 24 661.3 0 24 250 32 661.3 0 32 251 17 661.3 0 17 252 18 661.3 0 18 253 26 661.3 0 26 254 30 661.3 0 30 255 48 661.3 0 48 256 27 661.3 0 27 257 38 661.3 0 38 258 51 661.3 0 51 259 86 661.3 0 86 260 58 661.3 0 58 261 50 661.3 0 50 262 34 661.3 0 34 263 20 661.3 0 20 264 26 661.3 0 26 265 22 661.3 0 22 266 10 661.3 0 10 267 24 661.3 0 24 268 17 661.3 0 17 269 27 661.3 0 27 270 32 661.3 0 32 271 33 661.3 0 33 272 32 661.3 0 32 273 30 661.3 0 30 274 22 661.3 0 22 275 24 661.3 0 24 276 25 661.3 0 25 277 25 661.3 0 25 278 32 661.3 0 32 279 30 661.3 0 30 280 20 661.3 0 20 281 34 661.3 0 34 282 29 661.3 0 29 283 33 661.3 0 33 284 28 661.3 0 28 285 31 661.3 0 31 286 31 661.3 0 31 287 19 661.3 0 19 288 18 661.3 0 18 289 22 661.3 0 22 290 18 661.3 0 18 291 26 661.3 0 26 292 22 661.3 0 22 293 19 661.3 0 19 294 25 661.3 0 25 295 17 661.3 0 17 296 26 661.3 0 26 297 24 661.3 0 24 298 45 661.3 0 45 299 44 661.3 0 44 300 30 661.3 0 30 301 26 661.3 0 26 Finished in 1.43 s (50 us/read; 1.20 M reads/minute). === Summary === Total reads processed: 28,540 Reads with adapters: 12,538 (43.9%) Reads that were too short: 3 (0.0%) Reads written (passing filters): 12,535 (43.9%) Total basepairs processed: 7,528,409 bp Total written (filtered): 3,422,245 bp (45.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 12538 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 1 0.0 3 0 0 0 1 18 8 0.0 3 0 0 8 19 223 0.0 3 0 220 1 1 1 20 12252 0.0 4 12098 137 9 1 7 21 36 0.0 4 10 26 22 2 0.0 4 0 0 1 0 1 23 4 0.0 4 1 0 1 2 24 1 0.0 4 0 0 0 0 1 39 1 0.0 4 0 0 0 0 1 60 1 0.0 4 0 0 0 0 1 76 1 0.0 4 0 1 127 5 0.0 4 0 0 0 0 5 184 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.43 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 12,535 Reads with adapters: 1,172 (9.3%) Reads written (passing filters): 12,535 (100.0%) Total basepairs processed: 3,422,245 bp Total written (filtered): 3,218,141 bp (94.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1172 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 65.7% C: 0.4% G: 33.6% T: 0.2% none/other: 0.1% Overview of removed sequences length count expect max.err error counts 3 1 195.9 0 1 4 3 49.0 0 3 10 23 0.0 1 1 22 11 372 0.0 1 0 372 12 1 0.0 1 0 1 14 1 0.0 1 0 1 224 1 0.0 1 1 258 4 0.0 1 4 259 702 0.0 1 679 23 260 50 0.0 1 46 4 261 12 0.0 1 11 1 262 1 0.0 1 1 273 1 0.0 1 1