This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGTATA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 69.99 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 78,424 (2.9%) Reads that were too short: 26,666 (1.0%) Reads written (passing filters): 51,758 (1.9%) Total basepairs processed: 815,312,680 bp Total written (filtered): 10,352,459 bp (1.3%) === Adapter 1 === Sequence: TGTATA; Type: regular 5'; Length: 6; Trimmed: 78424 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 245 661.3 0 245 7 5265 661.3 0 5265 8 116 661.3 0 116 9 6130 661.3 0 6130 10 21 661.3 0 21 11 3 661.3 0 3 12 6 661.3 0 6 13 9 661.3 0 9 14 6 661.3 0 6 15 1 661.3 0 1 16 8 661.3 0 8 17 11 661.3 0 11 18 5 661.3 0 5 19 31 661.3 0 31 20 4 661.3 0 4 21 46 661.3 0 46 22 14 661.3 0 14 23 29 661.3 0 29 24 15 661.3 0 15 25 53 661.3 0 53 26 14 661.3 0 14 27 8 661.3 0 8 28 6 661.3 0 6 29 10 661.3 0 10 30 14 661.3 0 14 31 10 661.3 0 10 32 41 661.3 0 41 33 26 661.3 0 26 34 660 661.3 0 660 35 151 661.3 0 151 36 17 661.3 0 17 37 7 661.3 0 7 38 11 661.3 0 11 39 4 661.3 0 4 40 8 661.3 0 8 41 9 661.3 0 9 42 15 661.3 0 15 43 4 661.3 0 4 44 4 661.3 0 4 45 9 661.3 0 9 46 3 661.3 0 3 47 5 661.3 0 5 48 12 661.3 0 12 49 8 661.3 0 8 50 7 661.3 0 7 51 9 661.3 0 9 52 10 661.3 0 10 53 39 661.3 0 39 54 8 661.3 0 8 55 5 661.3 0 5 56 10 661.3 0 10 57 5 661.3 0 5 58 11 661.3 0 11 59 6 661.3 0 6 60 12 661.3 0 12 61 6 661.3 0 6 62 2 661.3 0 2 63 9 661.3 0 9 64 10 661.3 0 10 65 11 661.3 0 11 66 13 661.3 0 13 67 11 661.3 0 11 68 10 661.3 0 10 69 6 661.3 0 6 70 5 661.3 0 5 71 5 661.3 0 5 72 9 661.3 0 9 73 9 661.3 0 9 74 16 661.3 0 16 75 12 661.3 0 12 76 14 661.3 0 14 77 63 661.3 0 63 78 56 661.3 0 56 79 98 661.3 0 98 80 66 661.3 0 66 81 73 661.3 0 73 82 84 661.3 0 84 83 69 661.3 0 69 84 47 661.3 0 47 85 63 661.3 0 63 86 60 661.3 0 60 87 125 661.3 0 125 88 91 661.3 0 91 89 126 661.3 0 126 90 97 661.3 0 97 91 37 661.3 0 37 92 69 661.3 0 69 93 127 661.3 0 127 94 97 661.3 0 97 95 333 661.3 0 333 96 294 661.3 0 294 97 296 661.3 0 296 98 366 661.3 0 366 99 534 661.3 0 534 100 423 661.3 0 423 101 464 661.3 0 464 102 233 661.3 0 233 103 184 661.3 0 184 104 102 661.3 0 102 105 103 661.3 0 103 106 108 661.3 0 108 107 100 661.3 0 100 108 63 661.3 0 63 109 110 661.3 0 110 110 161 661.3 0 161 111 738 661.3 0 738 112 1720 661.3 0 1720 113 2345 661.3 0 2345 114 5141 661.3 0 5141 115 2472 661.3 0 2472 116 132 661.3 0 132 117 91 661.3 0 91 118 100 661.3 0 100 119 126 661.3 0 126 120 90 661.3 0 90 121 97 661.3 0 97 122 74 661.3 0 74 123 111 661.3 0 111 124 109 661.3 0 109 125 120 661.3 0 120 126 151 661.3 0 151 127 179 661.3 0 179 128 166 661.3 0 166 129 604 661.3 0 604 130 4902 661.3 0 4902 131 725 661.3 0 725 132 241 661.3 0 241 133 209 661.3 0 209 134 152 661.3 0 152 135 203 661.3 0 203 136 138 661.3 0 138 137 215 661.3 0 215 138 113 661.3 0 113 139 147 661.3 0 147 140 136 661.3 0 136 141 126 661.3 0 126 142 373 661.3 0 373 143 446 661.3 0 446 144 606 661.3 0 606 145 207 661.3 0 207 146 140 661.3 0 140 147 166 661.3 0 166 148 149 661.3 0 149 149 151 661.3 0 151 150 145 661.3 0 145 151 110 661.3 0 110 152 117 661.3 0 117 153 118 661.3 0 118 154 102 661.3 0 102 155 147 661.3 0 147 156 161 661.3 0 161 157 194 661.3 0 194 158 190 661.3 0 190 159 166 661.3 0 166 160 175 661.3 0 175 161 176 661.3 0 176 162 138 661.3 0 138 163 116 661.3 0 116 164 143 661.3 0 143 165 153 661.3 0 153 166 131 661.3 0 131 167 158 661.3 0 158 168 203 661.3 0 203 169 170 661.3 0 170 170 138 661.3 0 138 171 102 661.3 0 102 172 129 661.3 0 129 173 118 661.3 0 118 174 95 661.3 0 95 175 159 661.3 0 159 176 149 661.3 0 149 177 180 661.3 0 180 178 185 661.3 0 185 179 143 661.3 0 143 180 116 661.3 0 116 181 182 661.3 0 182 182 128 661.3 0 128 183 179 661.3 0 179 184 182 661.3 0 182 185 245 661.3 0 245 186 266 661.3 0 266 187 213 661.3 0 213 188 346 661.3 0 346 189 279 661.3 0 279 190 190 661.3 0 190 191 108 661.3 0 108 192 132 661.3 0 132 193 281 661.3 0 281 194 235 661.3 0 235 195 407 661.3 0 407 196 168 661.3 0 168 197 135 661.3 0 135 198 149 661.3 0 149 199 201 661.3 0 201 200 254 661.3 0 254 201 174 661.3 0 174 202 120 661.3 0 120 203 85 661.3 0 85 204 186 661.3 0 186 205 221 661.3 0 221 206 269 661.3 0 269 207 301 661.3 0 301 208 267 661.3 0 267 209 212 661.3 0 212 210 177 661.3 0 177 211 195 661.3 0 195 212 243 661.3 0 243 213 228 661.3 0 228 214 254 661.3 0 254 215 205 661.3 0 205 216 208 661.3 0 208 217 182 661.3 0 182 218 205 661.3 0 205 219 253 661.3 0 253 220 285 661.3 0 285 221 299 661.3 0 299 222 227 661.3 0 227 223 188 661.3 0 188 224 801 661.3 0 801 225 307 661.3 0 307 226 370 661.3 0 370 227 224 661.3 0 224 228 217 661.3 0 217 229 252 661.3 0 252 230 262 661.3 0 262 231 223 661.3 0 223 232 188 661.3 0 188 233 193 661.3 0 193 234 282 661.3 0 282 235 323 661.3 0 323 236 284 661.3 0 284 237 401 661.3 0 401 238 298 661.3 0 298 239 368 661.3 0 368 240 296 661.3 0 296 241 304 661.3 0 304 242 171 661.3 0 171 243 235 661.3 0 235 244 247 661.3 0 247 245 299 661.3 0 299 246 314 661.3 0 314 247 294 661.3 0 294 248 405 661.3 0 405 249 209 661.3 0 209 250 141 661.3 0 141 251 49 661.3 0 49 252 64 661.3 0 64 253 79 661.3 0 79 254 258 661.3 0 258 255 334 661.3 0 334 256 380 661.3 0 380 257 535 661.3 0 535 258 572 661.3 0 572 259 440 661.3 0 440 260 220 661.3 0 220 261 95 661.3 0 95 262 146 661.3 0 146 263 138 661.3 0 138 264 162 661.3 0 162 265 178 661.3 0 178 266 216 661.3 0 216 267 283 661.3 0 283 268 374 661.3 0 374 269 237 661.3 0 237 270 216 661.3 0 216 271 85 661.3 0 85 272 131 661.3 0 131 273 251 661.3 0 251 274 309 661.3 0 309 275 355 661.3 0 355 276 262 661.3 0 262 277 204 661.3 0 204 278 128 661.3 0 128 279 258 661.3 0 258 280 356 661.3 0 356 281 402 661.3 0 402 282 668 661.3 0 668 283 239 661.3 0 239 284 355 661.3 0 355 285 865 661.3 0 865 286 170 661.3 0 170 287 259 661.3 0 259 288 269 661.3 0 269 289 170 661.3 0 170 290 88 661.3 0 88 291 141 661.3 0 141 292 335 661.3 0 335 293 411 661.3 0 411 294 586 661.3 0 586 295 398 661.3 0 398 296 329 661.3 0 329 297 196 661.3 0 196 298 151 661.3 0 151 299 167 661.3 0 167 300 173 661.3 0 173 301 261 661.3 0 261 Finished in 1.93 s (37 us/read; 1.61 M reads/minute). === Summary === Total reads processed: 51,758 Reads with adapters: 6,388 (12.3%) Reads that were too short: 39 (0.1%) Reads written (passing filters): 6,349 (12.3%) Total basepairs processed: 10,352,459 bp Total written (filtered): 1,735,472 bp (16.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 6388 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 4 0.0 3 0 0 2 2 16 88 0.0 3 0 87 1 17 6152 0.0 3 5987 141 15 9 18 23 0.0 3 2 14 4 3 19 6 0.0 3 2 0 3 1 20 6 0.0 3 1 0 0 5 21 1 0.0 3 1 24 1 0.0 3 0 0 0 1 27 1 0.0 3 0 0 0 1 29 8 0.0 3 0 0 0 8 30 1 0.0 3 0 0 1 32 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 62 29 0.0 3 0 0 0 29 65 9 0.0 3 0 0 0 9 72 1 0.0 3 0 0 0 1 73 3 0.0 3 0 0 0 3 74 1 0.0 3 0 0 0 1 75 1 0.0 3 1 76 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 117 2 0.0 3 0 1 0 1 122 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 1 140 2 0.0 3 0 0 0 2 141 4 0.0 3 0 0 0 4 142 4 0.0 3 0 0 0 4 144 6 0.0 3 0 0 0 6 148 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 163 2 0.0 3 0 0 0 2 174 2 0.0 3 0 0 0 2 184 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 282 2 0.0 3 0 0 0 2 283 2 0.0 3 0 0 0 2 289 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.22 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 6,349 Reads with adapters: 403 (6.3%) Reads written (passing filters): 6,349 (100.0%) Total basepairs processed: 1,735,472 bp Total written (filtered): 1,734,255 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 403 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 46.2% C: 4.0% G: 46.2% T: 3.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 396 99.2 0 396 4 6 24.8 0 6 5 1 6.2 0 1