This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCTCCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 70.59 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 81,020 (3.0%) Reads that were too short: 5,147 (0.2%) Reads written (passing filters): 75,873 (2.8%) Total basepairs processed: 815,312,680 bp Total written (filtered): 17,852,009 bp (2.2%) === Adapter 1 === Sequence: GCTCCG; Type: regular 5'; Length: 6; Trimmed: 81020 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 513 661.3 0 513 7 7416 661.3 0 7416 8 1229 661.3 0 1229 9 35269 661.3 0 35269 10 70 661.3 0 70 11 19 661.3 0 19 12 17 661.3 0 17 13 23 661.3 0 23 14 13 661.3 0 13 15 18 661.3 0 18 16 11 661.3 0 11 17 13 661.3 0 13 18 25 661.3 0 25 19 21 661.3 0 21 20 17 661.3 0 17 21 18 661.3 0 18 22 19 661.3 0 19 23 20 661.3 0 20 24 12 661.3 0 12 25 18 661.3 0 18 26 23 661.3 0 23 27 34 661.3 0 34 28 17 661.3 0 17 29 18 661.3 0 18 30 25 661.3 0 25 31 23 661.3 0 23 32 29 661.3 0 29 33 39 661.3 0 39 34 29 661.3 0 29 35 12 661.3 0 12 36 20 661.3 0 20 37 34 661.3 0 34 38 26 661.3 0 26 39 31 661.3 0 31 40 33 661.3 0 33 41 16 661.3 0 16 42 23 661.3 0 23 43 9 661.3 0 9 44 16 661.3 0 16 45 15 661.3 0 15 46 21 661.3 0 21 47 25 661.3 0 25 48 13 661.3 0 13 49 11 661.3 0 11 50 13 661.3 0 13 51 14 661.3 0 14 52 10 661.3 0 10 53 15 661.3 0 15 54 15 661.3 0 15 55 37 661.3 0 37 56 22 661.3 0 22 57 25 661.3 0 25 58 18 661.3 0 18 59 27 661.3 0 27 60 35 661.3 0 35 61 12 661.3 0 12 62 23 661.3 0 23 63 22 661.3 0 22 64 11 661.3 0 11 65 7 661.3 0 7 66 19 661.3 0 19 67 19 661.3 0 19 68 23 661.3 0 23 69 10 661.3 0 10 70 13 661.3 0 13 71 24 661.3 0 24 72 18 661.3 0 18 73 12 661.3 0 12 74 13 661.3 0 13 75 15 661.3 0 15 76 13 661.3 0 13 77 10 661.3 0 10 78 14 661.3 0 14 79 12 661.3 0 12 80 14 661.3 0 14 81 19 661.3 0 19 82 15 661.3 0 15 83 19 661.3 0 19 84 16 661.3 0 16 85 27 661.3 0 27 86 11 661.3 0 11 87 18 661.3 0 18 88 14 661.3 0 14 89 21 661.3 0 21 90 19 661.3 0 19 91 8 661.3 0 8 92 16 661.3 0 16 93 18 661.3 0 18 94 12 661.3 0 12 95 21 661.3 0 21 96 33 661.3 0 33 97 21 661.3 0 21 98 14 661.3 0 14 99 16 661.3 0 16 100 7 661.3 0 7 101 13 661.3 0 13 102 12 661.3 0 12 103 33 661.3 0 33 104 29 661.3 0 29 105 36 661.3 0 36 106 69 661.3 0 69 107 86 661.3 0 86 108 7 661.3 0 7 109 17 661.3 0 17 110 17 661.3 0 17 111 18 661.3 0 18 112 26 661.3 0 26 113 18 661.3 0 18 114 46 661.3 0 46 115 26 661.3 0 26 116 64 661.3 0 64 117 25 661.3 0 25 118 16 661.3 0 16 119 22 661.3 0 22 120 24 661.3 0 24 121 19 661.3 0 19 122 18 661.3 0 18 123 25 661.3 0 25 124 17 661.3 0 17 125 16 661.3 0 16 126 18 661.3 0 18 127 29 661.3 0 29 128 77 661.3 0 77 129 40 661.3 0 40 130 49 661.3 0 49 131 29 661.3 0 29 132 76 661.3 0 76 133 86 661.3 0 86 134 166 661.3 0 166 135 46 661.3 0 46 136 63 661.3 0 63 137 136 661.3 0 136 138 99 661.3 0 99 139 72 661.3 0 72 140 122 661.3 0 122 141 102 661.3 0 102 142 124 661.3 0 124 143 118 661.3 0 118 144 123 661.3 0 123 145 177 661.3 0 177 146 242 661.3 0 242 147 2493 661.3 0 2493 148 14862 661.3 0 14862 149 5511 661.3 0 5511 150 283 661.3 0 283 151 58 661.3 0 58 152 31 661.3 0 31 153 33 661.3 0 33 154 62 661.3 0 62 155 31 661.3 0 31 156 22 661.3 0 22 157 21 661.3 0 21 158 24 661.3 0 24 159 22 661.3 0 22 160 21 661.3 0 21 161 21 661.3 0 21 162 21 661.3 0 21 163 17 661.3 0 17 164 28 661.3 0 28 165 15 661.3 0 15 166 18 661.3 0 18 167 19 661.3 0 19 168 35 661.3 0 35 169 62 661.3 0 62 170 66 661.3 0 66 171 21 661.3 0 21 172 47 661.3 0 47 173 28 661.3 0 28 174 48 661.3 0 48 175 69 661.3 0 69 176 13 661.3 0 13 177 19 661.3 0 19 178 39 661.3 0 39 179 33 661.3 0 33 180 15 661.3 0 15 181 18 661.3 0 18 182 20 661.3 0 20 183 37 661.3 0 37 184 38 661.3 0 38 185 24 661.3 0 24 186 28 661.3 0 28 187 29 661.3 0 29 188 229 661.3 0 229 189 86 661.3 0 86 190 164 661.3 0 164 191 44 661.3 0 44 192 27 661.3 0 27 193 15 661.3 0 15 194 19 661.3 0 19 195 18 661.3 0 18 196 37 661.3 0 37 197 40 661.3 0 40 198 44 661.3 0 44 199 112 661.3 0 112 200 230 661.3 0 230 201 1630 661.3 0 1630 202 1798 661.3 0 1798 203 595 661.3 0 595 204 210 661.3 0 210 205 11 661.3 0 11 206 13 661.3 0 13 207 20 661.3 0 20 208 18 661.3 0 18 209 11 661.3 0 11 210 13 661.3 0 13 211 13 661.3 0 13 212 18 661.3 0 18 213 14 661.3 0 14 214 13 661.3 0 13 215 12 661.3 0 12 216 25 661.3 0 25 217 8 661.3 0 8 218 11 661.3 0 11 219 12 661.3 0 12 220 6 661.3 0 6 221 14 661.3 0 14 222 17 661.3 0 17 223 20 661.3 0 20 224 33 661.3 0 33 225 183 661.3 0 183 226 26 661.3 0 26 227 23 661.3 0 23 228 102 661.3 0 102 229 66 661.3 0 66 230 57 661.3 0 57 231 17 661.3 0 17 232 19 661.3 0 19 233 8 661.3 0 8 234 16 661.3 0 16 235 17 661.3 0 17 236 25 661.3 0 25 237 6 661.3 0 6 238 35 661.3 0 35 239 10 661.3 0 10 240 16 661.3 0 16 241 14 661.3 0 14 242 28 661.3 0 28 243 29 661.3 0 29 244 23 661.3 0 23 245 24 661.3 0 24 246 12 661.3 0 12 247 11 661.3 0 11 248 14 661.3 0 14 249 18 661.3 0 18 250 17 661.3 0 17 251 21 661.3 0 21 252 20 661.3 0 20 253 25 661.3 0 25 254 30 661.3 0 30 255 7 661.3 0 7 256 15 661.3 0 15 257 21 661.3 0 21 258 62 661.3 0 62 259 37 661.3 0 37 260 62 661.3 0 62 261 11 661.3 0 11 262 13 661.3 0 13 263 18 661.3 0 18 264 23 661.3 0 23 265 22 661.3 0 22 266 17 661.3 0 17 267 24 661.3 0 24 268 28 661.3 0 28 269 42 661.3 0 42 270 22 661.3 0 22 271 39 661.3 0 39 272 17 661.3 0 17 273 21 661.3 0 21 274 30 661.3 0 30 275 33 661.3 0 33 276 39 661.3 0 39 277 58 661.3 0 58 278 70 661.3 0 70 279 81 661.3 0 81 280 50 661.3 0 50 281 22 661.3 0 22 282 34 661.3 0 34 283 41 661.3 0 41 284 15 661.3 0 15 285 16 661.3 0 16 286 21 661.3 0 21 287 11 661.3 0 11 288 18 661.3 0 18 289 30 661.3 0 30 290 14 661.3 0 14 291 22 661.3 0 22 292 28 661.3 0 28 293 34 661.3 0 34 294 46 661.3 0 46 295 16 661.3 0 16 296 39 661.3 0 39 297 18 661.3 0 18 298 1 661.3 0 1 299 17 661.3 0 17 300 12 661.3 0 12 301 13 661.3 0 13 Finished in 3.34 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 75,873 Reads with adapters: 38,509 (50.8%) Reads that were too short: 3 (0.0%) Reads written (passing filters): 38,506 (50.8%) Total basepairs processed: 17,852,009 bp Total written (filtered): 10,476,293 bp (58.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 38509 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 2 0.0 3 0 0 0 0 2 17 3 0.0 3 0 0 0 1 2 18 25 0.0 3 0 0 22 0 3 19 533 0.0 3 0 522 8 2 1 20 36400 0.0 4 35935 442 18 2 3 21 122 0.0 4 34 87 0 0 1 22 1399 0.0 4 1382 11 5 0 1 23 17 0.0 4 0 1 0 16 24 3 0.0 4 0 0 0 0 3 79 1 0.0 4 0 0 0 0 1 143 1 0.0 4 0 0 0 0 1 154 1 0.0 4 0 0 0 0 1 166 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.32 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 38,506 Reads with adapters: 2,981 (7.7%) Reads written (passing filters): 38,506 (100.0%) Total basepairs processed: 10,476,293 bp Total written (filtered): 9,937,738 bp (94.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2981 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 66.6% C: 2.3% G: 30.8% T: 0.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 601.7 0 1 4 3 150.4 0 3 6 5 9.4 0 5 9 167 0.1 0 7 160 10 539 0.0 1 19 520 11 207 0.0 1 0 207 12 1 0.0 1 0 1 13 1 0.0 1 0 1 150 1 0.0 1 0 1 240 1 0.0 1 1 256 27 0.0 1 25 2 257 1595 0.0 1 1477 118 258 332 0.0 1 319 13 259 34 0.0 1 32 2 260 22 0.0 1 21 1 261 2 0.0 1 2 263 13 0.0 1 12 1 264 2 0.0 1 2 265 21 0.0 1 20 1 266 2 0.0 1 2 269 2 0.0 1 2 270 2 0.0 1 2 272 1 0.0 1 1