This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACACAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 71.05 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 586,910 (21.7%) Reads that were too short: 215,698 (8.0%) Reads written (passing filters): 371,212 (13.7%) Total basepairs processed: 815,312,680 bp Total written (filtered): 68,813,724 bp (8.4%) === Adapter 1 === Sequence: ACACAA; Type: regular 5'; Length: 6; Trimmed: 586910 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 989 661.3 0 989 7 17195 661.3 0 17195 8 1027 661.3 0 1027 9 44638 661.3 0 44638 10 77 661.3 0 77 11 18 661.3 0 18 12 16 661.3 0 16 13 14 661.3 0 14 14 18 661.3 0 18 15 15 661.3 0 15 16 29 661.3 0 29 17 25 661.3 0 25 18 11 661.3 0 11 19 30 661.3 0 30 20 17 661.3 0 17 21 26 661.3 0 26 22 24 661.3 0 24 23 20 661.3 0 20 24 15 661.3 0 15 25 26 661.3 0 26 26 22 661.3 0 22 27 26 661.3 0 26 28 39 661.3 0 39 29 28 661.3 0 28 30 27 661.3 0 27 31 33 661.3 0 33 32 78 661.3 0 78 33 35 661.3 0 35 34 30 661.3 0 30 35 20 661.3 0 20 36 29 661.3 0 29 37 37 661.3 0 37 38 19 661.3 0 19 39 22 661.3 0 22 40 116 661.3 0 116 41 52 661.3 0 52 42 90 661.3 0 90 43 95 661.3 0 95 44 32 661.3 0 32 45 47 661.3 0 47 46 36 661.3 0 36 47 28 661.3 0 28 48 46 661.3 0 46 49 54 661.3 0 54 50 136 661.3 0 136 51 1518 661.3 0 1518 52 1401 661.3 0 1401 53 15 661.3 0 15 54 11 661.3 0 11 55 16 661.3 0 16 56 28 661.3 0 28 57 24 661.3 0 24 58 23 661.3 0 23 59 14 661.3 0 14 60 14 661.3 0 14 61 17 661.3 0 17 62 16 661.3 0 16 63 29 661.3 0 29 64 21 661.3 0 21 65 15 661.3 0 15 66 32 661.3 0 32 67 30 661.3 0 30 68 96 661.3 0 96 69 108 661.3 0 108 70 29 661.3 0 29 71 59 661.3 0 59 72 33 661.3 0 33 73 29 661.3 0 29 74 29 661.3 0 29 75 39 661.3 0 39 76 35 661.3 0 35 77 46 661.3 0 46 78 64 661.3 0 64 79 277 661.3 0 277 80 925 661.3 0 925 81 1264 661.3 0 1264 82 1935 661.3 0 1935 83 3053 661.3 0 3053 84 2955 661.3 0 2955 85 4673 661.3 0 4673 86 3346 661.3 0 3346 87 2150 661.3 0 2150 88 1696 661.3 0 1696 89 1464 661.3 0 1464 90 1350 661.3 0 1350 91 3976 661.3 0 3976 92 2073 661.3 0 2073 93 3047 661.3 0 3047 94 1798 661.3 0 1798 95 3474 661.3 0 3474 96 2448 661.3 0 2448 97 983 661.3 0 983 98 804 661.3 0 804 99 935 661.3 0 935 100 843 661.3 0 843 101 1572 661.3 0 1572 102 1899 661.3 0 1899 103 3113 661.3 0 3113 104 3962 661.3 0 3962 105 4938 661.3 0 4938 106 3427 661.3 0 3427 107 3275 661.3 0 3275 108 1762 661.3 0 1762 109 2740 661.3 0 2740 110 1855 661.3 0 1855 111 2691 661.3 0 2691 112 2762 661.3 0 2762 113 3104 661.3 0 3104 114 2747 661.3 0 2747 115 2545 661.3 0 2545 116 2180 661.3 0 2180 117 1949 661.3 0 1949 118 2899 661.3 0 2899 119 3215 661.3 0 3215 120 2866 661.3 0 2866 121 2495 661.3 0 2495 122 2209 661.3 0 2209 123 2373 661.3 0 2373 124 2782 661.3 0 2782 125 3578 661.3 0 3578 126 3491 661.3 0 3491 127 2684 661.3 0 2684 128 2463 661.3 0 2463 129 1912 661.3 0 1912 130 2242 661.3 0 2242 131 1943 661.3 0 1943 132 2534 661.3 0 2534 133 4940 661.3 0 4940 134 4359 661.3 0 4359 135 3449 661.3 0 3449 136 2213 661.3 0 2213 137 1944 661.3 0 1944 138 1880 661.3 0 1880 139 1965 661.3 0 1965 140 2192 661.3 0 2192 141 2139 661.3 0 2139 142 2002 661.3 0 2002 143 1990 661.3 0 1990 144 2141 661.3 0 2141 145 2559 661.3 0 2559 146 2815 661.3 0 2815 147 2637 661.3 0 2637 148 3317 661.3 0 3317 149 3185 661.3 0 3185 150 2668 661.3 0 2668 151 2779 661.3 0 2779 152 2771 661.3 0 2771 153 3224 661.3 0 3224 154 2972 661.3 0 2972 155 2573 661.3 0 2573 156 2439 661.3 0 2439 157 2222 661.3 0 2222 158 2251 661.3 0 2251 159 2550 661.3 0 2550 160 2698 661.3 0 2698 161 2948 661.3 0 2948 162 4349 661.3 0 4349 163 4227 661.3 0 4227 164 3625 661.3 0 3625 165 2837 661.3 0 2837 166 2026 661.3 0 2026 167 2080 661.3 0 2080 168 1752 661.3 0 1752 169 2170 661.3 0 2170 170 2417 661.3 0 2417 171 2478 661.3 0 2478 172 2734 661.3 0 2734 173 2801 661.3 0 2801 174 2303 661.3 0 2303 175 1790 661.3 0 1790 176 1737 661.3 0 1737 177 1601 661.3 0 1601 178 2274 661.3 0 2274 179 2368 661.3 0 2368 180 2370 661.3 0 2370 181 2410 661.3 0 2410 182 2106 661.3 0 2106 183 2354 661.3 0 2354 184 1973 661.3 0 1973 185 2131 661.3 0 2131 186 2223 661.3 0 2223 187 1952 661.3 0 1952 188 1928 661.3 0 1928 189 2172 661.3 0 2172 190 2591 661.3 0 2591 191 2778 661.3 0 2778 192 2631 661.3 0 2631 193 2580 661.3 0 2580 194 1657 661.3 0 1657 195 1270 661.3 0 1270 196 1334 661.3 0 1334 197 1499 661.3 0 1499 198 2648 661.3 0 2648 199 2713 661.3 0 2713 200 1780 661.3 0 1780 201 1847 661.3 0 1847 202 1587 661.3 0 1587 203 1984 661.3 0 1984 204 1676 661.3 0 1676 205 1576 661.3 0 1576 206 1793 661.3 0 1793 207 1816 661.3 0 1816 208 1684 661.3 0 1684 209 1653 661.3 0 1653 210 1512 661.3 0 1512 211 1830 661.3 0 1830 212 2677 661.3 0 2677 213 14136 661.3 0 14136 214 4156 661.3 0 4156 215 9784 661.3 0 9784 216 2277 661.3 0 2277 217 2077 661.3 0 2077 218 2123 661.3 0 2123 219 2182 661.3 0 2182 220 1920 661.3 0 1920 221 1681 661.3 0 1681 222 2051 661.3 0 2051 223 1830 661.3 0 1830 224 2111 661.3 0 2111 225 2491 661.3 0 2491 226 2290 661.3 0 2290 227 2724 661.3 0 2724 228 2126 661.3 0 2126 229 2439 661.3 0 2439 230 1954 661.3 0 1954 231 1807 661.3 0 1807 232 1493 661.3 0 1493 233 1455 661.3 0 1455 234 1606 661.3 0 1606 235 1897 661.3 0 1897 236 1782 661.3 0 1782 237 1685 661.3 0 1685 238 1876 661.3 0 1876 239 1809 661.3 0 1809 240 1732 661.3 0 1732 241 1525 661.3 0 1525 242 1697 661.3 0 1697 243 1595 661.3 0 1595 244 1335 661.3 0 1335 245 1608 661.3 0 1608 246 2024 661.3 0 2024 247 3285 661.3 0 3285 248 3223 661.3 0 3223 249 2656 661.3 0 2656 250 2549 661.3 0 2549 251 3336 661.3 0 3336 252 2069 661.3 0 2069 253 2172 661.3 0 2172 254 1710 661.3 0 1710 255 1859 661.3 0 1859 256 1711 661.3 0 1711 257 2000 661.3 0 2000 258 1954 661.3 0 1954 259 1792 661.3 0 1792 260 1456 661.3 0 1456 261 1322 661.3 0 1322 262 1314 661.3 0 1314 263 1583 661.3 0 1583 264 1960 661.3 0 1960 265 1959 661.3 0 1959 266 2384 661.3 0 2384 267 3083 661.3 0 3083 268 2451 661.3 0 2451 269 2332 661.3 0 2332 270 1524 661.3 0 1524 271 1929 661.3 0 1929 272 1493 661.3 0 1493 273 1897 661.3 0 1897 274 1871 661.3 0 1871 275 1819 661.3 0 1819 276 1633 661.3 0 1633 277 1518 661.3 0 1518 278 1664 661.3 0 1664 279 2361 661.3 0 2361 280 2102 661.3 0 2102 281 1242 661.3 0 1242 282 1727 661.3 0 1727 283 1393 661.3 0 1393 284 1800 661.3 0 1800 285 1634 661.3 0 1634 286 2016 661.3 0 2016 287 2468 661.3 0 2468 288 1850 661.3 0 1850 289 1742 661.3 0 1742 290 1729 661.3 0 1729 291 2225 661.3 0 2225 292 1808 661.3 0 1808 293 2342 661.3 0 2342 294 1917 661.3 0 1917 295 2139 661.3 0 2139 296 1597 661.3 0 1597 297 1273 661.3 0 1273 298 1030 661.3 0 1030 299 1108 661.3 0 1108 300 1370 661.3 0 1370 301 4251 661.3 0 4251 Finished in 13.49 s (36 us/read; 1.65 M reads/minute). === Summary === Total reads processed: 371,212 Reads with adapters: 18,061 (4.9%) Reads that were too short: 92 (0.0%) Reads written (passing filters): 17,969 (4.8%) Total basepairs processed: 68,813,724 bp Total written (filtered): 4,881,848 bp (7.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 18061 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 12 0.0 3 0 0 0 0 12 18 14 0.0 3 0 0 13 0 1 19 335 0.0 3 0 330 3 0 2 20 16871 0.0 4 16645 208 12 2 4 21 29 0.0 4 1 27 0 0 1 22 326 0.0 4 318 4 3 0 1 23 6 0.0 4 0 0 0 4 2 24 2 0.0 4 0 0 0 0 2 26 1 0.0 4 0 0 0 0 1 29 1 0.0 4 0 0 0 0 1 30 1 0.0 4 0 0 0 0 1 31 1 0.0 4 0 0 0 0 1 33 1 0.0 4 0 0 0 0 1 36 1 0.0 4 0 0 0 0 1 39 3 0.0 4 0 0 0 0 3 41 1 0.0 4 0 0 0 0 1 43 2 0.0 4 1 0 0 0 1 46 1 0.0 4 0 0 0 0 1 47 1 0.0 4 0 0 0 0 1 48 1 0.0 4 0 0 0 0 1 50 2 0.0 4 0 0 0 0 2 54 1 0.0 4 0 0 0 0 1 55 2 0.0 4 0 0 0 0 2 60 1 0.0 4 0 0 0 0 1 61 2 0.0 4 0 0 0 0 2 63 1 0.0 4 0 0 0 0 1 64 1 0.0 4 0 0 0 0 1 66 1 0.0 4 0 0 0 0 1 67 2 0.0 4 0 0 0 0 2 68 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 70 1 0.0 4 0 0 0 0 1 74 2 0.0 4 0 0 0 0 2 75 2 0.0 4 0 0 0 0 2 76 2 0.0 4 0 0 0 0 2 78 2 0.0 4 0 0 0 0 2 79 1 0.0 4 0 0 0 1 80 2 0.0 4 0 0 0 0 2 81 2 0.0 4 0 0 0 0 2 82 1 0.0 4 0 0 0 0 1 84 1 0.0 4 0 0 0 0 1 85 1 0.0 4 0 0 0 1 86 2 0.0 4 0 0 0 0 2 91 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 98 1 0.0 4 0 0 0 0 1 99 3 0.0 4 0 0 0 0 3 100 1 0.0 4 0 0 0 0 1 101 2 0.0 4 0 0 0 0 2 103 1 0.0 4 0 0 0 0 1 104 3 0.0 4 0 0 0 0 3 107 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 0 1 109 2 0.0 4 0 0 0 0 2 110 1 0.0 4 0 0 0 0 1 111 1 0.0 4 0 0 0 0 1 113 1 0.0 4 0 0 0 0 1 114 3 0.0 4 0 0 0 0 3 115 2 0.0 4 0 0 0 0 2 118 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 0 0 0 1 125 2 0.0 4 0 0 0 0 2 126 2 0.0 4 0 0 0 0 2 127 344 0.0 4 0 0 0 0 344 128 1 0.0 4 0 0 0 0 1 129 1 0.0 4 0 0 0 0 1 130 1 0.0 4 0 0 0 0 1 131 1 0.0 4 0 0 0 0 1 136 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 0 1 138 2 0.0 4 0 0 0 0 2 143 1 0.0 4 0 0 0 0 1 149 1 0.0 4 0 0 0 0 1 154 2 0.0 4 0 0 0 0 2 157 3 0.0 4 0 0 0 0 3 158 1 0.0 4 0 0 0 0 1 159 2 0.0 4 0 0 0 0 2 160 1 0.0 4 0 0 0 0 1 165 1 0.0 4 0 0 0 0 1 169 1 0.0 4 0 0 0 0 1 170 1 0.0 4 0 0 0 0 1 174 1 0.0 4 0 0 0 0 1 177 1 0.0 4 0 0 0 0 1 178 1 0.0 4 0 0 0 0 1 181 1 0.0 4 0 0 0 0 1 187 2 0.0 4 0 0 0 0 2 188 1 0.0 4 0 0 0 0 1 189 1 0.0 4 0 0 0 0 1 192 2 0.0 4 0 0 0 0 2 195 1 0.0 4 0 0 0 0 1 197 1 0.0 4 0 0 0 0 1 199 1 0.0 4 0 0 0 0 1 206 1 0.0 4 0 0 0 0 1 228 5 0.0 4 0 0 0 0 5 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.60 s (33 us/read; 1.80 M reads/minute). === Summary === Total reads processed: 17,969 Reads with adapters: 2,403 (13.4%) Reads written (passing filters): 17,969 (100.0%) Total basepairs processed: 4,881,848 bp Total written (filtered): 4,282,187 bp (87.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2403 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 95.3% C: 0.2% G: 4.2% T: 0.3% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "A" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 7 280.8 0 7 10 1 0.0 1 0 1 11 96 0.0 1 0 96 12 6 0.0 1 0 6 171 2 0.0 1 2 260 3 0.0 1 3 261 2154 0.0 1 2057 97 262 122 0.0 1 117 5 263 2 0.0 1 1 1 264 2 0.0 1 2 269 1 0.0 1 1 270 1 0.0 1 1 271 3 0.0 1 2 1 272 3 0.0 1 3 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.