This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCACT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 71.73 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 200,853 (7.4%) Reads that were too short: 22,854 (0.8%) Reads written (passing filters): 177,999 (6.6%) Total basepairs processed: 815,312,680 bp Total written (filtered): 35,114,769 bp (4.3%) === Adapter 1 === Sequence: AGCACT; Type: regular 5'; Length: 6; Trimmed: 200853 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 278 661.3 0 278 7 13270 661.3 0 13270 8 18651 661.3 0 18651 9 43 661.3 0 43 10 23 661.3 0 23 11 24 661.3 0 24 12 36 661.3 0 36 13 25 661.3 0 25 14 425 661.3 0 425 15 94 661.3 0 94 16 22 661.3 0 22 17 25 661.3 0 25 18 25 661.3 0 25 19 24 661.3 0 24 20 22 661.3 0 22 21 23 661.3 0 23 22 37 661.3 0 37 23 30 661.3 0 30 24 25 661.3 0 25 25 51 661.3 0 51 26 74 661.3 0 74 27 104 661.3 0 104 28 29 661.3 0 29 29 35 661.3 0 35 30 45 661.3 0 45 31 22 661.3 0 22 32 33 661.3 0 33 33 33 661.3 0 33 34 28 661.3 0 28 35 30 661.3 0 30 36 30 661.3 0 30 37 32 661.3 0 32 38 31 661.3 0 31 39 24 661.3 0 24 40 35 661.3 0 35 41 30 661.3 0 30 42 28 661.3 0 28 43 45 661.3 0 45 44 30 661.3 0 30 45 17 661.3 0 17 46 24 661.3 0 24 47 36 661.3 0 36 48 31 661.3 0 31 49 26 661.3 0 26 50 40 661.3 0 40 51 24 661.3 0 24 52 26 661.3 0 26 53 32 661.3 0 32 54 40 661.3 0 40 55 57 661.3 0 57 56 40 661.3 0 40 57 22 661.3 0 22 58 32 661.3 0 32 59 28 661.3 0 28 60 50 661.3 0 50 61 33 661.3 0 33 62 32 661.3 0 32 63 41 661.3 0 41 64 30 661.3 0 30 65 22 661.3 0 22 66 27 661.3 0 27 67 32 661.3 0 32 68 65 661.3 0 65 69 21 661.3 0 21 70 24 661.3 0 24 71 28 661.3 0 28 72 29 661.3 0 29 73 31 661.3 0 31 74 26 661.3 0 26 75 25 661.3 0 25 76 25 661.3 0 25 77 27 661.3 0 27 78 31 661.3 0 31 79 37 661.3 0 37 80 77 661.3 0 77 81 83 661.3 0 83 82 76 661.3 0 76 83 37 661.3 0 37 84 63 661.3 0 63 85 54 661.3 0 54 86 114 661.3 0 114 87 172 661.3 0 172 88 172 661.3 0 172 89 202 661.3 0 202 90 332 661.3 0 332 91 712 661.3 0 712 92 192 661.3 0 192 93 391 661.3 0 391 94 479 661.3 0 479 95 318 661.3 0 318 96 264 661.3 0 264 97 346 661.3 0 346 98 449 661.3 0 449 99 403 661.3 0 403 100 395 661.3 0 395 101 436 661.3 0 436 102 554 661.3 0 554 103 822 661.3 0 822 104 13158 661.3 0 13158 105 29983 661.3 0 29983 106 36375 661.3 0 36375 107 164 661.3 0 164 108 47 661.3 0 47 109 34 661.3 0 34 110 70 661.3 0 70 111 120 661.3 0 120 112 92 661.3 0 92 113 109 661.3 0 109 114 61 661.3 0 61 115 42 661.3 0 42 116 75 661.3 0 75 117 65 661.3 0 65 118 57 661.3 0 57 119 135 661.3 0 135 120 110 661.3 0 110 121 86 661.3 0 86 122 85 661.3 0 85 123 80 661.3 0 80 124 82 661.3 0 82 125 136 661.3 0 136 126 169 661.3 0 169 127 154 661.3 0 154 128 97 661.3 0 97 129 112 661.3 0 112 130 112 661.3 0 112 131 76 661.3 0 76 132 123 661.3 0 123 133 82 661.3 0 82 134 81 661.3 0 81 135 65 661.3 0 65 136 58 661.3 0 58 137 99 661.3 0 99 138 143 661.3 0 143 139 169 661.3 0 169 140 134 661.3 0 134 141 126 661.3 0 126 142 334 661.3 0 334 143 116 661.3 0 116 144 156 661.3 0 156 145 128 661.3 0 128 146 170 661.3 0 170 147 194 661.3 0 194 148 129 661.3 0 129 149 104 661.3 0 104 150 81 661.3 0 81 151 168 661.3 0 168 152 173 661.3 0 173 153 256 661.3 0 256 154 240 661.3 0 240 155 303 661.3 0 303 156 368 661.3 0 368 157 670 661.3 0 670 158 1564 661.3 0 1564 159 20876 661.3 0 20876 160 3716 661.3 0 3716 161 15194 661.3 0 15194 162 171 661.3 0 171 163 149 661.3 0 149 164 99 661.3 0 99 165 102 661.3 0 102 166 118 661.3 0 118 167 259 661.3 0 259 168 154 661.3 0 154 169 223 661.3 0 223 170 88 661.3 0 88 171 131 661.3 0 131 172 209 661.3 0 209 173 178 661.3 0 178 174 196 661.3 0 196 175 79 661.3 0 79 176 77 661.3 0 77 177 89 661.3 0 89 178 108 661.3 0 108 179 129 661.3 0 129 180 136 661.3 0 136 181 196 661.3 0 196 182 129 661.3 0 129 183 126 661.3 0 126 184 317 661.3 0 317 185 467 661.3 0 467 186 308 661.3 0 308 187 185 661.3 0 185 188 142 661.3 0 142 189 364 661.3 0 364 190 133 661.3 0 133 191 101 661.3 0 101 192 112 661.3 0 112 193 105 661.3 0 105 194 90 661.3 0 90 195 112 661.3 0 112 196 102 661.3 0 102 197 101 661.3 0 101 198 141 661.3 0 141 199 156 661.3 0 156 200 1513 661.3 0 1513 201 373 661.3 0 373 202 285 661.3 0 285 203 120 661.3 0 120 204 108 661.3 0 108 205 146 661.3 0 146 206 155 661.3 0 155 207 146 661.3 0 146 208 121 661.3 0 121 209 111 661.3 0 111 210 103 661.3 0 103 211 96 661.3 0 96 212 116 661.3 0 116 213 134 661.3 0 134 214 130 661.3 0 130 215 273 661.3 0 273 216 129 661.3 0 129 217 174 661.3 0 174 218 124 661.3 0 124 219 181 661.3 0 181 220 215 661.3 0 215 221 243 661.3 0 243 222 114 661.3 0 114 223 171 661.3 0 171 224 177 661.3 0 177 225 166 661.3 0 166 226 175 661.3 0 175 227 158 661.3 0 158 228 279 661.3 0 279 229 224 661.3 0 224 230 227 661.3 0 227 231 146 661.3 0 146 232 155 661.3 0 155 233 156 661.3 0 156 234 119 661.3 0 119 235 144 661.3 0 144 236 186 661.3 0 186 237 161 661.3 0 161 238 209 661.3 0 209 239 327 661.3 0 327 240 1819 661.3 0 1819 241 1222 661.3 0 1222 242 660 661.3 0 660 243 315 661.3 0 315 244 182 661.3 0 182 245 126 661.3 0 126 246 106 661.3 0 106 247 130 661.3 0 130 248 134 661.3 0 134 249 174 661.3 0 174 250 172 661.3 0 172 251 143 661.3 0 143 252 181 661.3 0 181 253 229 661.3 0 229 254 194 661.3 0 194 255 228 661.3 0 228 256 204 661.3 0 204 257 167 661.3 0 167 258 154 661.3 0 154 259 154 661.3 0 154 260 119 661.3 0 119 261 130 661.3 0 130 262 137 661.3 0 137 263 187 661.3 0 187 264 153 661.3 0 153 265 229 661.3 0 229 266 141 661.3 0 141 267 125 661.3 0 125 268 262 661.3 0 262 269 312 661.3 0 312 270 195 661.3 0 195 271 151 661.3 0 151 272 169 661.3 0 169 273 231 661.3 0 231 274 276 661.3 0 276 275 239 661.3 0 239 276 267 661.3 0 267 277 221 661.3 0 221 278 361 661.3 0 361 279 248 661.3 0 248 280 295 661.3 0 295 281 569 661.3 0 569 282 485 661.3 0 485 283 708 661.3 0 708 284 289 661.3 0 289 285 156 661.3 0 156 286 122 661.3 0 122 287 117 661.3 0 117 288 172 661.3 0 172 289 165 661.3 0 165 290 207 661.3 0 207 291 180 661.3 0 180 292 147 661.3 0 147 293 139 661.3 0 139 294 145 661.3 0 145 295 106 661.3 0 106 296 99 661.3 0 99 297 161 661.3 0 161 298 153 661.3 0 153 299 262 661.3 0 262 300 626 661.3 0 626 301 300 661.3 0 300 Finished in 7.34 s (41 us/read; 1.46 M reads/minute). === Summary === Total reads processed: 177,999 Reads with adapters: 27,414 (15.4%) Reads that were too short: 12 (0.0%) Reads written (passing filters): 27,402 (15.4%) Total basepairs processed: 35,114,769 bp Total written (filtered): 6,614,624 bp (18.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 27414 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 1 2 18 16 0.0 3 0 0 11 2 3 19 210 0.0 3 0 208 2 20 19003 0.0 4 18758 231 12 1 1 21 47 0.0 4 3 44 23 8 0.0 4 0 0 0 7 1 26 1 0.0 4 1 27 1 0.0 4 1 29 3 0.0 4 0 0 0 0 3 30 8076 0.0 4 0 0 0 0 8076 33 2 0.0 4 0 0 0 0 2 41 1 0.0 4 0 0 0 0 1 55 1 0.0 4 0 0 0 0 1 68 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 76 1 0.0 4 0 0 0 0 1 85 1 0.0 4 1 92 2 0.0 4 0 0 0 0 2 97 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 0 1 127 25 0.0 4 0 0 0 0 25 136 1 0.0 4 0 0 0 1 138 1 0.0 4 0 0 0 0 1 147 1 0.0 4 0 0 0 0 1 167 1 0.0 4 0 0 0 0 1 170 1 0.0 4 0 0 0 0 1 185 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.86 s (31 us/read; 1.91 M reads/minute). === Summary === Total reads processed: 27,402 Reads with adapters: 797 (2.9%) Reads written (passing filters): 27,402 (100.0%) Total basepairs processed: 6,614,624 bp Total written (filtered): 6,605,282 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 797 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 9.0% C: 0.5% G: 89.8% T: 0.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 14 428.2 0 14 4 61 107.0 0 61 5 7 26.8 0 7 9 2 0.1 0 0 2 10 83 0.0 1 6 77 11 625 0.0 1 2 623 259 2 0.0 1 2 260 3 0.0 1 3 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.