This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTAGTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 70.06 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 101,959 (3.8%) Reads that were too short: 7,042 (0.3%) Reads written (passing filters): 94,917 (3.5%) Total basepairs processed: 815,312,680 bp Total written (filtered): 25,440,692 bp (3.1%) === Adapter 1 === Sequence: GTAGTG; Type: regular 5'; Length: 6; Trimmed: 101959 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1599 661.3 0 1599 7 49309 661.3 0 49309 8 585 661.3 0 585 9 22226 661.3 0 22226 10 37 661.3 0 37 11 22 661.3 0 22 12 13 661.3 0 13 13 17 661.3 0 17 14 17 661.3 0 17 15 16 661.3 0 16 16 19 661.3 0 19 17 28 661.3 0 28 18 20 661.3 0 20 19 16 661.3 0 16 20 23 661.3 0 23 21 15 661.3 0 15 22 32 661.3 0 32 23 23 661.3 0 23 24 23 661.3 0 23 25 32 661.3 0 32 26 48 661.3 0 48 27 33 661.3 0 33 28 112 661.3 0 112 29 33 661.3 0 33 30 116 661.3 0 116 31 60 661.3 0 60 32 17 661.3 0 17 33 14 661.3 0 14 34 18 661.3 0 18 35 20 661.3 0 20 36 22 661.3 0 22 37 20 661.3 0 20 38 22 661.3 0 22 39 38 661.3 0 38 40 23 661.3 0 23 41 12 661.3 0 12 42 28 661.3 0 28 43 37 661.3 0 37 44 28 661.3 0 28 45 24 661.3 0 24 46 16 661.3 0 16 47 23 661.3 0 23 48 17 661.3 0 17 49 47 661.3 0 47 50 30 661.3 0 30 51 220 661.3 0 220 52 34 661.3 0 34 53 32 661.3 0 32 54 20 661.3 0 20 55 14 661.3 0 14 56 19 661.3 0 19 57 41 661.3 0 41 58 38 661.3 0 38 59 30 661.3 0 30 60 19 661.3 0 19 61 30 661.3 0 30 62 29 661.3 0 29 63 21 661.3 0 21 64 25 661.3 0 25 65 29 661.3 0 29 66 31 661.3 0 31 67 32 661.3 0 32 68 72 661.3 0 72 69 691 661.3 0 691 70 164 661.3 0 164 71 427 661.3 0 427 72 29 661.3 0 29 73 14 661.3 0 14 74 21 661.3 0 21 75 15 661.3 0 15 76 31 661.3 0 31 77 152 661.3 0 152 78 77 661.3 0 77 79 98 661.3 0 98 80 24 661.3 0 24 81 26 661.3 0 26 82 26 661.3 0 26 83 27 661.3 0 27 84 19 661.3 0 19 85 22 661.3 0 22 86 20 661.3 0 20 87 23 661.3 0 23 88 26 661.3 0 26 89 20 661.3 0 20 90 34 661.3 0 34 91 24 661.3 0 24 92 19 661.3 0 19 93 32 661.3 0 32 94 37 661.3 0 37 95 33 661.3 0 33 96 34 661.3 0 34 97 38 661.3 0 38 98 89 661.3 0 89 99 206 661.3 0 206 100 48 661.3 0 48 101 68 661.3 0 68 102 37 661.3 0 37 103 33 661.3 0 33 104 45 661.3 0 45 105 43 661.3 0 43 106 38 661.3 0 38 107 54 661.3 0 54 108 45 661.3 0 45 109 44 661.3 0 44 110 46 661.3 0 46 111 47 661.3 0 47 112 72 661.3 0 72 113 71 661.3 0 71 114 219 661.3 0 219 115 2271 661.3 0 2271 116 2420 661.3 0 2420 117 41 661.3 0 41 118 37 661.3 0 37 119 22 661.3 0 22 120 24 661.3 0 24 121 27 661.3 0 27 122 21 661.3 0 21 123 29 661.3 0 29 124 31 661.3 0 31 125 33 661.3 0 33 126 50 661.3 0 50 127 71 661.3 0 71 128 49 661.3 0 49 129 54 661.3 0 54 130 78 661.3 0 78 131 59 661.3 0 59 132 60 661.3 0 60 133 84 661.3 0 84 134 78 661.3 0 78 135 89 661.3 0 89 136 62 661.3 0 62 137 95 661.3 0 95 138 294 661.3 0 294 139 731 661.3 0 731 140 689 661.3 0 689 141 1550 661.3 0 1550 142 440 661.3 0 440 143 685 661.3 0 685 144 812 661.3 0 812 145 808 661.3 0 808 146 34 661.3 0 34 147 42 661.3 0 42 148 375 661.3 0 375 149 253 661.3 0 253 150 320 661.3 0 320 151 836 661.3 0 836 152 298 661.3 0 298 153 24 661.3 0 24 154 124 661.3 0 124 155 34 661.3 0 34 156 40 661.3 0 40 157 42 661.3 0 42 158 20 661.3 0 20 159 33 661.3 0 33 160 29 661.3 0 29 161 18 661.3 0 18 162 27 661.3 0 27 163 17 661.3 0 17 164 36 661.3 0 36 165 25 661.3 0 25 166 28 661.3 0 28 167 33 661.3 0 33 168 26 661.3 0 26 169 35 661.3 0 35 170 38 661.3 0 38 171 18 661.3 0 18 172 24 661.3 0 24 173 31 661.3 0 31 174 28 661.3 0 28 175 28 661.3 0 28 176 40 661.3 0 40 177 24 661.3 0 24 178 32 661.3 0 32 179 34 661.3 0 34 180 25 661.3 0 25 181 33 661.3 0 33 182 38 661.3 0 38 183 29 661.3 0 29 184 53 661.3 0 53 185 43 661.3 0 43 186 51 661.3 0 51 187 27 661.3 0 27 188 39 661.3 0 39 189 85 661.3 0 85 190 65 661.3 0 65 191 128 661.3 0 128 192 56 661.3 0 56 193 49 661.3 0 49 194 45 661.3 0 45 195 38 661.3 0 38 196 49 661.3 0 49 197 48 661.3 0 48 198 22 661.3 0 22 199 21 661.3 0 21 200 19 661.3 0 19 201 40 661.3 0 40 202 25 661.3 0 25 203 36 661.3 0 36 204 40 661.3 0 40 205 36 661.3 0 36 206 44 661.3 0 44 207 35 661.3 0 35 208 25 661.3 0 25 209 59 661.3 0 59 210 68 661.3 0 68 211 54 661.3 0 54 212 26 661.3 0 26 213 41 661.3 0 41 214 55 661.3 0 55 215 44 661.3 0 44 216 40 661.3 0 40 217 44 661.3 0 44 218 26 661.3 0 26 219 22 661.3 0 22 220 37 661.3 0 37 221 37 661.3 0 37 222 56 661.3 0 56 223 36 661.3 0 36 224 32 661.3 0 32 225 57 661.3 0 57 226 45 661.3 0 45 227 35 661.3 0 35 228 23 661.3 0 23 229 27 661.3 0 27 230 25 661.3 0 25 231 41 661.3 0 41 232 40 661.3 0 40 233 52 661.3 0 52 234 72 661.3 0 72 235 81 661.3 0 81 236 65 661.3 0 65 237 45 661.3 0 45 238 55 661.3 0 55 239 60 661.3 0 60 240 57 661.3 0 57 241 58 661.3 0 58 242 46 661.3 0 46 243 38 661.3 0 38 244 63 661.3 0 63 245 48 661.3 0 48 246 34 661.3 0 34 247 32 661.3 0 32 248 41 661.3 0 41 249 219 661.3 0 219 250 206 661.3 0 206 251 404 661.3 0 404 252 22 661.3 0 22 253 74 661.3 0 74 254 149 661.3 0 149 255 195 661.3 0 195 256 53 661.3 0 53 257 31 661.3 0 31 258 48 661.3 0 48 259 32 661.3 0 32 260 42 661.3 0 42 261 46 661.3 0 46 262 40 661.3 0 40 263 43 661.3 0 43 264 41 661.3 0 41 265 52 661.3 0 52 266 29 661.3 0 29 267 29 661.3 0 29 268 41 661.3 0 41 269 397 661.3 0 397 270 636 661.3 0 636 271 45 661.3 0 45 272 31 661.3 0 31 273 58 661.3 0 58 274 73 661.3 0 73 275 71 661.3 0 71 276 41 661.3 0 41 277 45 661.3 0 45 278 47 661.3 0 47 279 22 661.3 0 22 280 28 661.3 0 28 281 60 661.3 0 60 282 92 661.3 0 92 283 47 661.3 0 47 284 42 661.3 0 42 285 69 661.3 0 69 286 50 661.3 0 50 287 40 661.3 0 40 288 64 661.3 0 64 289 132 661.3 0 132 290 56 661.3 0 56 291 62 661.3 0 62 292 88 661.3 0 88 293 65 661.3 0 65 294 52 661.3 0 52 295 57 661.3 0 57 296 90 661.3 0 90 297 82 661.3 0 82 298 116 661.3 0 116 299 107 661.3 0 107 300 167 661.3 0 167 301 156 661.3 0 156 Finished in 4.59 s (48 us/read; 1.24 M reads/minute). === Summary === Total reads processed: 94,917 Reads with adapters: 51,125 (53.9%) Reads that were too short: 5 (0.0%) Reads written (passing filters): 51,120 (53.9%) Total basepairs processed: 25,440,692 bp Total written (filtered): 13,953,962 bp (54.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 51125 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 0 3 18 20 0.0 3 0 0 20 19 688 0.0 3 0 667 18 2 1 20 49759 0.0 4 49196 535 25 3 21 118 0.0 4 27 89 2 22 10 0.0 4 3 0 7 23 17 0.0 4 0 0 0 17 24 2 0.0 4 0 0 0 0 2 25 1 0.0 4 0 0 0 0 1 31 1 0.0 4 0 0 0 0 1 32 1 0.0 4 0 1 39 1 0.0 4 0 1 126 10 0.0 4 0 0 0 0 10 127 489 0.0 4 0 0 0 0 489 128 2 0.0 4 0 0 0 0 2 203 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 268 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.73 s (34 us/read; 1.77 M reads/minute). === Summary === Total reads processed: 51,120 Reads with adapters: 346 (0.7%) Reads written (passing filters): 51,120 (100.0%) Total basepairs processed: 13,953,962 bp Total written (filtered): 13,939,885 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 346 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 13.6% C: 0.6% G: 83.2% T: 2.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 8 798.8 0 8 4 1 199.7 0 1 5 2 49.9 0 2 6 1 12.5 0 1 10 2 0.0 1 0 2 11 268 0.0 1 3 265 12 20 0.0 1 1 19 13 1 0.0 1 0 1 14 1 0.0 1 0 1 210 4 0.0 1 0 4 261 29 0.0 1 27 2 262 3 0.0 1 3 263 3 0.0 1 3 269 1 0.0 1 1 272 1 0.0 1 1 273 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.