This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATGAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 72.14 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 237,975 (8.8%) Reads that were too short: 77,609 (2.9%) Reads written (passing filters): 160,366 (5.9%) Total basepairs processed: 815,312,680 bp Total written (filtered): 30,756,400 bp (3.8%) === Adapter 1 === Sequence: AATGAA; Type: regular 5'; Length: 6; Trimmed: 237975 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 216 661.3 0 216 7 348 661.3 0 348 8 7963 661.3 0 7963 9 16924 661.3 0 16924 10 105 661.3 0 105 11 53 661.3 0 53 12 35 661.3 0 35 13 53 661.3 0 53 14 44 661.3 0 44 15 31 661.3 0 31 16 39 661.3 0 39 17 33 661.3 0 33 18 36 661.3 0 36 19 31 661.3 0 31 20 41 661.3 0 41 21 42 661.3 0 42 22 44 661.3 0 44 23 52 661.3 0 52 24 52 661.3 0 52 25 46 661.3 0 46 26 38 661.3 0 38 27 28 661.3 0 28 28 45 661.3 0 45 29 27 661.3 0 27 30 37 661.3 0 37 31 94 661.3 0 94 32 56 661.3 0 56 33 33 661.3 0 33 34 29 661.3 0 29 35 55 661.3 0 55 36 35 661.3 0 35 37 29 661.3 0 29 38 47 661.3 0 47 39 39 661.3 0 39 40 46 661.3 0 46 41 39 661.3 0 39 42 63 661.3 0 63 43 46 661.3 0 46 44 44 661.3 0 44 45 58 661.3 0 58 46 56 661.3 0 56 47 61 661.3 0 61 48 29 661.3 0 29 49 28 661.3 0 28 50 34 661.3 0 34 51 30 661.3 0 30 52 20 661.3 0 20 53 25 661.3 0 25 54 26 661.3 0 26 55 33 661.3 0 33 56 36 661.3 0 36 57 41 661.3 0 41 58 76 661.3 0 76 59 78 661.3 0 78 60 55 661.3 0 55 61 37 661.3 0 37 62 66 661.3 0 66 63 59 661.3 0 59 64 53 661.3 0 53 65 46 661.3 0 46 66 101 661.3 0 101 67 59 661.3 0 59 68 93 661.3 0 93 69 95 661.3 0 95 70 86 661.3 0 86 71 245 661.3 0 245 72 689 661.3 0 689 73 535 661.3 0 535 74 565 661.3 0 565 75 482 661.3 0 482 76 833 661.3 0 833 77 2737 661.3 0 2737 78 943 661.3 0 943 79 2243 661.3 0 2243 80 3277 661.3 0 3277 81 3990 661.3 0 3990 82 2067 661.3 0 2067 83 2147 661.3 0 2147 84 2025 661.3 0 2025 85 1309 661.3 0 1309 86 1983 661.3 0 1983 87 879 661.3 0 879 88 598 661.3 0 598 89 485 661.3 0 485 90 893 661.3 0 893 91 915 661.3 0 915 92 859 661.3 0 859 93 1058 661.3 0 1058 94 1131 661.3 0 1131 95 1185 661.3 0 1185 96 1259 661.3 0 1259 97 1035 661.3 0 1035 98 502 661.3 0 502 99 777 661.3 0 777 100 2329 661.3 0 2329 101 1432 661.3 0 1432 102 711 661.3 0 711 103 1015 661.3 0 1015 104 670 661.3 0 670 105 283 661.3 0 283 106 169 661.3 0 169 107 368 661.3 0 368 108 777 661.3 0 777 109 886 661.3 0 886 110 791 661.3 0 791 111 896 661.3 0 896 112 898 661.3 0 898 113 881 661.3 0 881 114 1005 661.3 0 1005 115 1266 661.3 0 1266 116 1532 661.3 0 1532 117 1212 661.3 0 1212 118 1036 661.3 0 1036 119 899 661.3 0 899 120 978 661.3 0 978 121 996 661.3 0 996 122 834 661.3 0 834 123 809 661.3 0 809 124 986 661.3 0 986 125 1292 661.3 0 1292 126 973 661.3 0 973 127 921 661.3 0 921 128 1054 661.3 0 1054 129 1180 661.3 0 1180 130 1417 661.3 0 1417 131 1359 661.3 0 1359 132 1000 661.3 0 1000 133 698 661.3 0 698 134 826 661.3 0 826 135 793 661.3 0 793 136 1289 661.3 0 1289 137 1563 661.3 0 1563 138 1206 661.3 0 1206 139 870 661.3 0 870 140 1028 661.3 0 1028 141 1434 661.3 0 1434 142 1104 661.3 0 1104 143 936 661.3 0 936 144 958 661.3 0 958 145 1109 661.3 0 1109 146 1147 661.3 0 1147 147 791 661.3 0 791 148 941 661.3 0 941 149 985 661.3 0 985 150 818 661.3 0 818 151 681 661.3 0 681 152 804 661.3 0 804 153 833 661.3 0 833 154 922 661.3 0 922 155 829 661.3 0 829 156 771 661.3 0 771 157 858 661.3 0 858 158 1093 661.3 0 1093 159 1274 661.3 0 1274 160 1862 661.3 0 1862 161 631 661.3 0 631 162 746 661.3 0 746 163 704 661.3 0 704 164 727 661.3 0 727 165 819 661.3 0 819 166 986 661.3 0 986 167 757 661.3 0 757 168 696 661.3 0 696 169 765 661.3 0 765 170 837 661.3 0 837 171 700 661.3 0 700 172 563 661.3 0 563 173 629 661.3 0 629 174 737 661.3 0 737 175 687 661.3 0 687 176 689 661.3 0 689 177 705 661.3 0 705 178 691 661.3 0 691 179 763 661.3 0 763 180 666 661.3 0 666 181 685 661.3 0 685 182 1725 661.3 0 1725 183 1243 661.3 0 1243 184 1359 661.3 0 1359 185 782 661.3 0 782 186 881 661.3 0 881 187 766 661.3 0 766 188 536 661.3 0 536 189 558 661.3 0 558 190 731 661.3 0 731 191 821 661.3 0 821 192 869 661.3 0 869 193 1041 661.3 0 1041 194 912 661.3 0 912 195 456 661.3 0 456 196 504 661.3 0 504 197 1058 661.3 0 1058 198 1588 661.3 0 1588 199 1186 661.3 0 1186 200 466 661.3 0 466 201 378 661.3 0 378 202 673 661.3 0 673 203 1058 661.3 0 1058 204 951 661.3 0 951 205 822 661.3 0 822 206 801 661.3 0 801 207 838 661.3 0 838 208 750 661.3 0 750 209 655 661.3 0 655 210 786 661.3 0 786 211 781 661.3 0 781 212 796 661.3 0 796 213 721 661.3 0 721 214 595 661.3 0 595 215 620 661.3 0 620 216 575 661.3 0 575 217 545 661.3 0 545 218 749 661.3 0 749 219 878 661.3 0 878 220 572 661.3 0 572 221 486 661.3 0 486 222 581 661.3 0 581 223 759 661.3 0 759 224 695 661.3 0 695 225 593 661.3 0 593 226 560 661.3 0 560 227 783 661.3 0 783 228 771 661.3 0 771 229 624 661.3 0 624 230 497 661.3 0 497 231 643 661.3 0 643 232 604 661.3 0 604 233 588 661.3 0 588 234 715 661.3 0 715 235 1086 661.3 0 1086 236 797 661.3 0 797 237 580 661.3 0 580 238 786 661.3 0 786 239 900 661.3 0 900 240 616 661.3 0 616 241 540 661.3 0 540 242 732 661.3 0 732 243 792 661.3 0 792 244 570 661.3 0 570 245 527 661.3 0 527 246 808 661.3 0 808 247 866 661.3 0 866 248 771 661.3 0 771 249 430 661.3 0 430 250 505 661.3 0 505 251 601 661.3 0 601 252 1159 661.3 0 1159 253 1116 661.3 0 1116 254 1410 661.3 0 1410 255 1225 661.3 0 1225 256 1218 661.3 0 1218 257 696 661.3 0 696 258 712 661.3 0 712 259 551 661.3 0 551 260 491 661.3 0 491 261 595 661.3 0 595 262 647 661.3 0 647 263 752 661.3 0 752 264 729 661.3 0 729 265 628 661.3 0 628 266 603 661.3 0 603 267 611 661.3 0 611 268 642 661.3 0 642 269 441 661.3 0 441 270 504 661.3 0 504 271 905 661.3 0 905 272 1129 661.3 0 1129 273 967 661.3 0 967 274 1075 661.3 0 1075 275 753 661.3 0 753 276 377 661.3 0 377 277 753 661.3 0 753 278 1073 661.3 0 1073 279 1073 661.3 0 1073 280 693 661.3 0 693 281 421 661.3 0 421 282 498 661.3 0 498 283 582 661.3 0 582 284 608 661.3 0 608 285 754 661.3 0 754 286 1118 661.3 0 1118 287 703 661.3 0 703 288 444 661.3 0 444 289 1039 661.3 0 1039 290 777 661.3 0 777 291 1119 661.3 0 1119 292 1267 661.3 0 1267 293 959 661.3 0 959 294 691 661.3 0 691 295 845 661.3 0 845 296 1022 661.3 0 1022 297 1150 661.3 0 1150 298 1025 661.3 0 1025 299 1416 661.3 0 1416 300 1370 661.3 0 1370 301 1301 661.3 0 1301 Finished in 5.55 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 160,366 Reads with adapters: 7,788 (4.9%) Reads that were too short: 71 (0.0%) Reads written (passing filters): 7,717 (4.8%) Total basepairs processed: 30,756,400 bp Total written (filtered): 2,116,305 bp (6.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 7788 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 2 0.0 3 0 0 1 1 16 87 0.0 3 0 78 4 5 17 7481 0.0 3 7296 150 23 12 18 35 0.0 3 3 18 8 6 19 17 0.0 3 3 1 4 9 20 7 0.0 3 0 0 0 7 21 1 0.0 3 0 0 0 1 22 1 0.0 3 1 24 1 0.0 3 0 0 0 1 25 10 0.0 3 1 0 0 9 27 1 0.0 3 0 0 0 1 28 6 0.0 3 0 0 0 6 30 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 36 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 39 2 0.0 3 0 0 0 2 40 1 0.0 3 0 0 0 1 41 4 0.0 3 0 0 0 4 44 2 0.0 3 0 0 1 1 50 2 0.0 3 0 0 0 2 51 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 55 3 0.0 3 0 0 1 2 57 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 67 4 0.0 3 0 0 0 4 68 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 75 3 0.0 3 0 0 0 3 76 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 89 2 0.0 3 0 0 0 2 91 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 104 2 0.0 3 0 0 0 2 105 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 112 11 0.0 3 0 0 1 10 113 5 0.0 3 0 0 0 5 115 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 125 2 0.0 3 0 0 0 2 126 5 0.0 3 0 0 0 5 129 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 143 2 0.0 3 0 0 1 1 147 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 172 2 0.0 3 0 0 0 2 175 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 1 185 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 200 1 0.0 3 0 0 0 1 205 2 0.0 3 0 0 0 2 213 1 0.0 3 0 0 0 1 214 19 0.0 3 0 0 2 17 216 2 0.0 3 0 0 0 2 217 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 224 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 283 2 0.0 3 0 0 0 2 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.28 s (36 us/read; 1.65 M reads/minute). === Summary === Total reads processed: 7,717 Reads with adapters: 139 (1.8%) Reads written (passing filters): 7,717 (100.0%) Total basepairs processed: 2,116,305 bp Total written (filtered): 2,115,883 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 139 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 55.4% C: 9.4% G: 26.6% T: 8.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 134 120.6 0 134 4 5 30.1 0 5