This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCAGAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 71.20 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 214,877 (7.9%) Reads that were too short: 19,058 (0.7%) Reads written (passing filters): 195,819 (7.2%) Total basepairs processed: 815,312,680 bp Total written (filtered): 56,088,595 bp (6.9%) === Adapter 1 === Sequence: TCAGAG; Type: regular 5'; Length: 6; Trimmed: 214877 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1583 661.3 0 1583 7 3452 661.3 0 3452 8 173405 661.3 0 173405 9 7607 661.3 0 7607 10 25 661.3 0 25 11 3 661.3 0 3 12 8 661.3 0 8 13 11 661.3 0 11 14 3 661.3 0 3 15 4 661.3 0 4 16 4 661.3 0 4 17 9 661.3 0 9 19 2 661.3 0 2 20 6 661.3 0 6 21 4 661.3 0 4 22 5 661.3 0 5 23 3 661.3 0 3 24 5 661.3 0 5 25 6 661.3 0 6 26 8 661.3 0 8 27 6 661.3 0 6 28 9 661.3 0 9 29 6 661.3 0 6 30 8 661.3 0 8 31 3 661.3 0 3 32 6 661.3 0 6 33 12 661.3 0 12 34 15 661.3 0 15 35 3 661.3 0 3 36 4 661.3 0 4 37 5 661.3 0 5 38 5 661.3 0 5 39 7 661.3 0 7 40 5 661.3 0 5 41 16 661.3 0 16 42 5 661.3 0 5 43 8 661.3 0 8 44 8 661.3 0 8 45 13 661.3 0 13 46 1 661.3 0 1 47 8 661.3 0 8 48 7 661.3 0 7 49 8 661.3 0 8 50 6 661.3 0 6 51 6 661.3 0 6 52 4 661.3 0 4 53 2 661.3 0 2 54 9 661.3 0 9 55 31 661.3 0 31 56 5 661.3 0 5 57 9 661.3 0 9 58 18 661.3 0 18 59 35 661.3 0 35 60 179 661.3 0 179 61 81 661.3 0 81 62 353 661.3 0 353 63 7 661.3 0 7 64 8 661.3 0 8 65 13 661.3 0 13 66 52 661.3 0 52 67 18 661.3 0 18 68 37 661.3 0 37 69 5 661.3 0 5 70 8 661.3 0 8 71 5 661.3 0 5 72 10 661.3 0 10 73 7 661.3 0 7 74 3 661.3 0 3 75 8 661.3 0 8 76 16 661.3 0 16 77 104 661.3 0 104 78 11 661.3 0 11 79 39 661.3 0 39 80 8 661.3 0 8 81 6 661.3 0 6 82 3 661.3 0 3 83 9 661.3 0 9 84 10 661.3 0 10 85 7 661.3 0 7 86 14 661.3 0 14 87 9 661.3 0 9 88 9 661.3 0 9 89 13 661.3 0 13 90 19 661.3 0 19 91 20 661.3 0 20 92 8 661.3 0 8 93 23 661.3 0 23 94 30 661.3 0 30 95 30 661.3 0 30 96 11 661.3 0 11 97 166 661.3 0 166 98 77 661.3 0 77 99 72 661.3 0 72 100 27 661.3 0 27 101 12 661.3 0 12 102 23 661.3 0 23 103 12 661.3 0 12 104 23 661.3 0 23 105 31 661.3 0 31 106 15 661.3 0 15 107 25 661.3 0 25 108 21 661.3 0 21 109 22 661.3 0 22 110 14 661.3 0 14 111 85 661.3 0 85 112 55 661.3 0 55 113 94 661.3 0 94 114 327 661.3 0 327 115 370 661.3 0 370 116 490 661.3 0 490 117 85 661.3 0 85 118 23 661.3 0 23 119 33 661.3 0 33 120 18 661.3 0 18 121 27 661.3 0 27 122 35 661.3 0 35 123 26 661.3 0 26 124 21 661.3 0 21 125 39 661.3 0 39 126 30 661.3 0 30 127 45 661.3 0 45 128 43 661.3 0 43 129 36 661.3 0 36 130 47 661.3 0 47 131 29 661.3 0 29 132 30 661.3 0 30 133 39 661.3 0 39 134 50 661.3 0 50 135 53 661.3 0 53 136 43 661.3 0 43 137 51 661.3 0 51 138 62 661.3 0 62 139 93 661.3 0 93 140 38 661.3 0 38 141 46 661.3 0 46 142 32 661.3 0 32 143 68 661.3 0 68 144 47 661.3 0 47 145 56 661.3 0 56 146 54 661.3 0 54 147 33 661.3 0 33 148 49 661.3 0 49 149 53 661.3 0 53 150 46 661.3 0 46 151 57 661.3 0 57 152 38 661.3 0 38 153 43 661.3 0 43 154 70 661.3 0 70 155 44 661.3 0 44 156 53 661.3 0 53 157 58 661.3 0 58 158 44 661.3 0 44 159 79 661.3 0 79 160 43 661.3 0 43 161 60 661.3 0 60 162 149 661.3 0 149 163 88 661.3 0 88 164 97 661.3 0 97 165 134 661.3 0 134 166 112 661.3 0 112 167 152 661.3 0 152 168 64 661.3 0 64 169 111 661.3 0 111 170 60 661.3 0 60 171 71 661.3 0 71 172 76 661.3 0 76 173 58 661.3 0 58 174 55 661.3 0 55 175 51 661.3 0 51 176 52 661.3 0 52 177 86 661.3 0 86 178 69 661.3 0 69 179 207 661.3 0 207 180 128 661.3 0 128 181 94 661.3 0 94 182 71 661.3 0 71 183 53 661.3 0 53 184 100 661.3 0 100 185 96 661.3 0 96 186 100 661.3 0 100 187 80 661.3 0 80 188 59 661.3 0 59 189 67 661.3 0 67 190 152 661.3 0 152 191 401 661.3 0 401 192 193 661.3 0 193 193 163 661.3 0 163 194 147 661.3 0 147 195 55 661.3 0 55 196 128 661.3 0 128 197 90 661.3 0 90 198 54 661.3 0 54 199 87 661.3 0 87 200 43 661.3 0 43 201 97 661.3 0 97 202 91 661.3 0 91 203 200 661.3 0 200 204 153 661.3 0 153 205 160 661.3 0 160 206 63 661.3 0 63 207 71 661.3 0 71 208 73 661.3 0 73 209 82 661.3 0 82 210 82 661.3 0 82 211 85 661.3 0 85 212 76 661.3 0 76 213 67 661.3 0 67 214 76 661.3 0 76 215 91 661.3 0 91 216 88 661.3 0 88 217 163 661.3 0 163 218 103 661.3 0 103 219 103 661.3 0 103 220 90 661.3 0 90 221 66 661.3 0 66 222 72 661.3 0 72 223 40 661.3 0 40 224 72 661.3 0 72 225 91 661.3 0 91 226 94 661.3 0 94 227 101 661.3 0 101 228 85 661.3 0 85 229 106 661.3 0 106 230 97 661.3 0 97 231 129 661.3 0 129 232 160 661.3 0 160 233 104 661.3 0 104 234 133 661.3 0 133 235 158 661.3 0 158 236 263 661.3 0 263 237 275 661.3 0 275 238 201 661.3 0 201 239 159 661.3 0 159 240 113 661.3 0 113 241 97 661.3 0 97 242 108 661.3 0 108 243 113 661.3 0 113 244 118 661.3 0 118 245 1834 661.3 0 1834 246 1345 661.3 0 1345 247 3522 661.3 0 3522 248 104 661.3 0 104 249 138 661.3 0 138 250 53 661.3 0 53 251 49 661.3 0 49 252 36 661.3 0 36 253 43 661.3 0 43 254 124 661.3 0 124 255 125 661.3 0 125 256 109 661.3 0 109 257 115 661.3 0 115 258 99 661.3 0 99 259 115 661.3 0 115 260 221 661.3 0 221 261 170 661.3 0 170 262 118 661.3 0 118 263 116 661.3 0 116 264 200 661.3 0 200 265 329 661.3 0 329 266 301 661.3 0 301 267 1325 661.3 0 1325 268 113 661.3 0 113 269 133 661.3 0 133 270 135 661.3 0 135 271 88 661.3 0 88 272 107 661.3 0 107 273 58 661.3 0 58 274 58 661.3 0 58 275 63 661.3 0 63 276 81 661.3 0 81 277 125 661.3 0 125 278 90 661.3 0 90 279 77 661.3 0 77 280 136 661.3 0 136 281 103 661.3 0 103 282 105 661.3 0 105 283 71 661.3 0 71 284 77 661.3 0 77 285 139 661.3 0 139 286 148 661.3 0 148 287 170 661.3 0 170 288 159 661.3 0 159 289 142 661.3 0 142 290 88 661.3 0 88 291 66 661.3 0 66 292 57 661.3 0 57 293 118 661.3 0 118 294 153 661.3 0 153 295 237 661.3 0 237 296 145 661.3 0 145 297 107 661.3 0 107 298 78 661.3 0 78 299 74 661.3 0 74 300 73 661.3 0 73 301 21 661.3 0 21 Finished in 10.11 s (52 us/read; 1.16 M reads/minute). === Summary === Total reads processed: 195,819 Reads with adapters: 10,362 (5.3%) Reads that were too short: 22 (0.0%) Reads written (passing filters): 10,340 (5.3%) Total basepairs processed: 56,088,595 bp Total written (filtered): 2,571,721 bp (4.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 10362 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 23 0.0 3 0 0 0 1 22 18 5 0.0 3 0 0 4 0 1 19 128 0.0 3 0 123 2 0 3 20 7619 0.0 4 7506 113 21 35 0.0 4 15 18 2 22 251 0.0 4 247 1 0 0 3 28 1 0.0 4 0 0 0 0 1 55 1 0.0 4 0 0 0 0 1 64 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 110 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 125 2 0.0 4 0 0 0 0 2 126 25 0.0 4 0 0 0 0 25 127 2231 0.0 4 0 0 0 0 2231 128 5 0.0 4 0 0 0 0 5 130 2 0.0 4 0 0 0 0 2 133 1 0.0 4 0 0 0 0 1 148 1 0.0 4 0 0 0 0 1 153 5 0.0 4 0 0 0 0 5 172 2 0.0 4 0 0 0 0 2 174 1 0.0 4 1 183 1 0.0 4 0 0 0 0 1 228 3 0.0 4 0 0 0 0 3 229 1 0.0 4 0 0 0 0 1 232 1 0.0 4 0 0 0 0 1 251 1 0.0 4 0 0 0 0 1 255 3 0.0 4 0 0 0 0 3 256 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 1 275 1 0.0 4 0 0 0 0 1 280 1 0.0 4 0 0 0 0 1 282 2 0.0 4 0 0 0 0 2 284 1 0.0 4 1 288 1 0.0 4 0 0 0 0 1 293 1 0.0 4 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.34 s (33 us/read; 1.82 M reads/minute). === Summary === Total reads processed: 10,340 Reads with adapters: 365 (3.5%) Reads written (passing filters): 10,340 (100.0%) Total basepairs processed: 2,571,721 bp Total written (filtered): 2,528,594 bp (98.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 365 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 8.2% C: 34.0% G: 51.0% T: 6.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 161.6 0 1 6 1 2.5 0 1 9 19 0.0 0 0 19 10 137 0.0 1 7 130 11 34 0.0 1 0 34 222 1 0.0 1 1 223 102 0.0 1 99 3 224 4 0.0 1 4 226 1 0.0 1 1 256 7 0.0 1 7 257 2 0.0 1 1 1 259 22 0.0 1 20 2 260 5 0.0 1 4 1 261 2 0.0 1 2 267 1 0.0 1 1 270 26 0.0 1 25 1