This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGACCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 70.28 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 47,333 (1.7%) Reads that were too short: 23,870 (0.9%) Reads written (passing filters): 23,463 (0.9%) Total basepairs processed: 815,312,680 bp Total written (filtered): 5,079,123 bp (0.6%) === Adapter 1 === Sequence: AGACCG; Type: regular 5'; Length: 6; Trimmed: 47333 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 259 661.3 0 259 7 8798 661.3 0 8798 8 41 661.3 0 41 9 22 661.3 0 22 10 28 661.3 0 28 11 19 661.3 0 19 12 80 661.3 0 80 13 42 661.3 0 42 14 16 661.3 0 16 15 13 661.3 0 13 16 28 661.3 0 28 17 21 661.3 0 21 18 16 661.3 0 16 19 12 661.3 0 12 20 17 661.3 0 17 21 19 661.3 0 19 22 13 661.3 0 13 23 18 661.3 0 18 24 15 661.3 0 15 25 15 661.3 0 15 26 25 661.3 0 25 27 23 661.3 0 23 28 16 661.3 0 16 29 27 661.3 0 27 30 28 661.3 0 28 31 18 661.3 0 18 32 44 661.3 0 44 33 23 661.3 0 23 34 20 661.3 0 20 35 31 661.3 0 31 36 13 661.3 0 13 37 17 661.3 0 17 38 10 661.3 0 10 39 19 661.3 0 19 40 19 661.3 0 19 41 22 661.3 0 22 42 22 661.3 0 22 43 10 661.3 0 10 44 16 661.3 0 16 45 10 661.3 0 10 46 28 661.3 0 28 47 13 661.3 0 13 48 25 661.3 0 25 49 23 661.3 0 23 50 23 661.3 0 23 51 18 661.3 0 18 52 18 661.3 0 18 53 23 661.3 0 23 54 24 661.3 0 24 55 42 661.3 0 42 56 36 661.3 0 36 57 18 661.3 0 18 58 16 661.3 0 16 59 34 661.3 0 34 60 15 661.3 0 15 61 23 661.3 0 23 62 28 661.3 0 28 63 20 661.3 0 20 64 21 661.3 0 21 65 19 661.3 0 19 66 26 661.3 0 26 67 26 661.3 0 26 68 25 661.3 0 25 69 246 661.3 0 246 70 32 661.3 0 32 71 61 661.3 0 61 72 44 661.3 0 44 73 41 661.3 0 41 74 192 661.3 0 192 75 92 661.3 0 92 76 123 661.3 0 123 77 32 661.3 0 32 78 38 661.3 0 38 79 56 661.3 0 56 80 65 661.3 0 65 81 117 661.3 0 117 82 282 661.3 0 282 83 272 661.3 0 272 84 640 661.3 0 640 85 88 661.3 0 88 86 62 661.3 0 62 87 46 661.3 0 46 88 94 661.3 0 94 89 63 661.3 0 63 90 35 661.3 0 35 91 57 661.3 0 57 92 124 661.3 0 124 93 68 661.3 0 68 94 41 661.3 0 41 95 50 661.3 0 50 96 25 661.3 0 25 97 41 661.3 0 41 98 29 661.3 0 29 99 25 661.3 0 25 100 26 661.3 0 26 101 36 661.3 0 36 102 29 661.3 0 29 103 24 661.3 0 24 104 21 661.3 0 21 105 15 661.3 0 15 106 21 661.3 0 21 107 20 661.3 0 20 108 18 661.3 0 18 109 32 661.3 0 32 110 34 661.3 0 34 111 27 661.3 0 27 112 51 661.3 0 51 113 50 661.3 0 50 114 42 661.3 0 42 115 36 661.3 0 36 116 42 661.3 0 42 117 56 661.3 0 56 118 59 661.3 0 59 119 71 661.3 0 71 120 52 661.3 0 52 121 61 661.3 0 61 122 95 661.3 0 95 123 163 661.3 0 163 124 55 661.3 0 55 125 90 661.3 0 90 126 59 661.3 0 59 127 122 661.3 0 122 128 93 661.3 0 93 129 44 661.3 0 44 130 48 661.3 0 48 131 58 661.3 0 58 132 72 661.3 0 72 133 68 661.3 0 68 134 65 661.3 0 65 135 61 661.3 0 61 136 66 661.3 0 66 137 72 661.3 0 72 138 64 661.3 0 64 139 86 661.3 0 86 140 60 661.3 0 60 141 48 661.3 0 48 142 59 661.3 0 59 143 97 661.3 0 97 144 65 661.3 0 65 145 57 661.3 0 57 146 39 661.3 0 39 147 64 661.3 0 64 148 45 661.3 0 45 149 72 661.3 0 72 150 53 661.3 0 53 151 70 661.3 0 70 152 63 661.3 0 63 153 88 661.3 0 88 154 95 661.3 0 95 155 69 661.3 0 69 156 69 661.3 0 69 157 68 661.3 0 68 158 42 661.3 0 42 159 78 661.3 0 78 160 58 661.3 0 58 161 41 661.3 0 41 162 79 661.3 0 79 163 99 661.3 0 99 164 104 661.3 0 104 165 76 661.3 0 76 166 96 661.3 0 96 167 536 661.3 0 536 168 239 661.3 0 239 169 710 661.3 0 710 170 128 661.3 0 128 171 529 661.3 0 529 172 211 661.3 0 211 173 202 661.3 0 202 174 82 661.3 0 82 175 56 661.3 0 56 176 59 661.3 0 59 177 65 661.3 0 65 178 69 661.3 0 69 179 63 661.3 0 63 180 68 661.3 0 68 181 75 661.3 0 75 182 74 661.3 0 74 183 82 661.3 0 82 184 178 661.3 0 178 185 91 661.3 0 91 186 40 661.3 0 40 187 40 661.3 0 40 188 122 661.3 0 122 189 179 661.3 0 179 190 127 661.3 0 127 191 77 661.3 0 77 192 71 661.3 0 71 193 34 661.3 0 34 194 50 661.3 0 50 195 86 661.3 0 86 196 120 661.3 0 120 197 62 661.3 0 62 198 62 661.3 0 62 199 59 661.3 0 59 200 327 661.3 0 327 201 802 661.3 0 802 202 352 661.3 0 352 203 118 661.3 0 118 204 93 661.3 0 93 205 77 661.3 0 77 206 89 661.3 0 89 207 48 661.3 0 48 208 54 661.3 0 54 209 53 661.3 0 53 210 70 661.3 0 70 211 87 661.3 0 87 212 70 661.3 0 70 213 87 661.3 0 87 214 83 661.3 0 83 215 141 661.3 0 141 216 149 661.3 0 149 217 105 661.3 0 105 218 71 661.3 0 71 219 64 661.3 0 64 220 70 661.3 0 70 221 89 661.3 0 89 222 114 661.3 0 114 223 113 661.3 0 113 224 142 661.3 0 142 225 175 661.3 0 175 226 268 661.3 0 268 227 612 661.3 0 612 228 3453 661.3 0 3453 229 1482 661.3 0 1482 230 2756 661.3 0 2756 231 172 661.3 0 172 232 99 661.3 0 99 233 68 661.3 0 68 234 90 661.3 0 90 235 55 661.3 0 55 236 65 661.3 0 65 237 76 661.3 0 76 238 85 661.3 0 85 239 72 661.3 0 72 240 98 661.3 0 98 241 44 661.3 0 44 242 73 661.3 0 73 243 117 661.3 0 117 244 158 661.3 0 158 245 118 661.3 0 118 246 77 661.3 0 77 247 153 661.3 0 153 248 79 661.3 0 79 249 103 661.3 0 103 250 187 661.3 0 187 251 227 661.3 0 227 252 90 661.3 0 90 253 123 661.3 0 123 254 106 661.3 0 106 255 102 661.3 0 102 256 181 661.3 0 181 257 195 661.3 0 195 258 350 661.3 0 350 259 82 661.3 0 82 260 116 661.3 0 116 261 576 661.3 0 576 262 866 661.3 0 866 263 1204 661.3 0 1204 264 356 661.3 0 356 265 307 661.3 0 307 266 89 661.3 0 89 267 66 661.3 0 66 268 203 661.3 0 203 269 764 661.3 0 764 270 1049 661.3 0 1049 271 221 661.3 0 221 272 150 661.3 0 150 273 51 661.3 0 51 274 45 661.3 0 45 275 49 661.3 0 49 276 77 661.3 0 77 277 77 661.3 0 77 278 106 661.3 0 106 279 53 661.3 0 53 280 65 661.3 0 65 281 87 661.3 0 87 282 232 661.3 0 232 283 289 661.3 0 289 284 144 661.3 0 144 285 84 661.3 0 84 286 60 661.3 0 60 287 70 661.3 0 70 288 65 661.3 0 65 289 74 661.3 0 74 290 119 661.3 0 119 291 125 661.3 0 125 292 153 661.3 0 153 293 119 661.3 0 119 294 113 661.3 0 113 295 34 661.3 0 34 296 46 661.3 0 46 297 92 661.3 0 92 298 132 661.3 0 132 299 205 661.3 0 205 300 518 661.3 0 518 301 89 661.3 0 89 Finished in 0.93 s (40 us/read; 1.51 M reads/minute). === Summary === Total reads processed: 23,463 Reads with adapters: 8,985 (38.3%) Reads that were too short: 9 (0.0%) Reads written (passing filters): 8,976 (38.3%) Total basepairs processed: 5,079,123 bp Total written (filtered): 2,484,715 bp (48.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 8985 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 1 16 105 0.0 3 0 100 5 17 8818 0.0 3 8631 162 21 4 18 21 0.0 3 2 13 4 2 19 11 0.0 3 0 0 6 5 20 6 0.0 3 0 0 0 6 22 2 0.0 3 0 0 0 2 24 1 0.0 3 0 0 0 1 25 1 0.0 3 0 0 0 1 27 2 0.0 3 0 0 0 2 43 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 88 3 0.0 3 0 0 0 3 89 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 108 2 0.0 3 0 0 0 2 124 2 0.0 3 0 0 0 2 125 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 215 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.32 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 8,976 Reads with adapters: 854 (9.5%) Reads written (passing filters): 8,976 (100.0%) Total basepairs processed: 2,484,715 bp Total written (filtered): 2,482,137 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 854 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 27.5% C: 64.5% G: 4.1% T: 3.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 840 140.2 0 840 4 12 35.1 0 12 5 2 8.8 0 2