This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTCATG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 70.08 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 73,480 (2.7%) Reads that were too short: 9,595 (0.4%) Reads written (passing filters): 63,885 (2.4%) Total basepairs processed: 815,312,680 bp Total written (filtered): 16,344,834 bp (2.0%) === Adapter 1 === Sequence: CTCATG; Type: regular 5'; Length: 6; Trimmed: 73480 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 718 661.3 0 718 7 34221 661.3 0 34221 8 314 661.3 0 314 9 8641 661.3 0 8641 10 30 661.3 0 30 11 18 661.3 0 18 12 21 661.3 0 21 13 22 661.3 0 22 14 19 661.3 0 19 15 16 661.3 0 16 16 36 661.3 0 36 17 19 661.3 0 19 18 29 661.3 0 29 19 18 661.3 0 18 20 16 661.3 0 16 21 22 661.3 0 22 22 14 661.3 0 14 23 29 661.3 0 29 24 30 661.3 0 30 25 41 661.3 0 41 26 31 661.3 0 31 27 19 661.3 0 19 28 24 661.3 0 24 29 23 661.3 0 23 30 29 661.3 0 29 31 35 661.3 0 35 32 28 661.3 0 28 33 37 661.3 0 37 34 41 661.3 0 41 35 18 661.3 0 18 36 35 661.3 0 35 37 31 661.3 0 31 38 28 661.3 0 28 39 19 661.3 0 19 40 39 661.3 0 39 41 18 661.3 0 18 42 20 661.3 0 20 43 41 661.3 0 41 44 128 661.3 0 128 45 30 661.3 0 30 46 134 661.3 0 134 47 17 661.3 0 17 48 300 661.3 0 300 49 27 661.3 0 27 50 19 661.3 0 19 51 24 661.3 0 24 52 30 661.3 0 30 53 84 661.3 0 84 54 31 661.3 0 31 55 24 661.3 0 24 56 58 661.3 0 58 57 55 661.3 0 55 58 78 661.3 0 78 59 49 661.3 0 49 60 57 661.3 0 57 61 35 661.3 0 35 62 26 661.3 0 26 63 25 661.3 0 25 64 26 661.3 0 26 65 27 661.3 0 27 66 30 661.3 0 30 67 28 661.3 0 28 68 24 661.3 0 24 69 20 661.3 0 20 70 25 661.3 0 25 71 29 661.3 0 29 72 15 661.3 0 15 73 28 661.3 0 28 74 26 661.3 0 26 75 45 661.3 0 45 76 23 661.3 0 23 77 23 661.3 0 23 78 21 661.3 0 21 79 24 661.3 0 24 80 32 661.3 0 32 81 28 661.3 0 28 82 34 661.3 0 34 83 34 661.3 0 34 84 30 661.3 0 30 85 29 661.3 0 29 86 22 661.3 0 22 87 29 661.3 0 29 88 24 661.3 0 24 89 31 661.3 0 31 90 27 661.3 0 27 91 39 661.3 0 39 92 44 661.3 0 44 93 50 661.3 0 50 94 43 661.3 0 43 95 42 661.3 0 42 96 42 661.3 0 42 97 95 661.3 0 95 98 94 661.3 0 94 99 43 661.3 0 43 100 57 661.3 0 57 101 36 661.3 0 36 102 35 661.3 0 35 103 33 661.3 0 33 104 61 661.3 0 61 105 135 661.3 0 135 106 1580 661.3 0 1580 107 283 661.3 0 283 108 339 661.3 0 339 109 44 661.3 0 44 110 35 661.3 0 35 111 39 661.3 0 39 112 48 661.3 0 48 113 53 661.3 0 53 114 204 661.3 0 204 115 198 661.3 0 198 116 516 661.3 0 516 117 44 661.3 0 44 118 37 661.3 0 37 119 67 661.3 0 67 120 53 661.3 0 53 121 49 661.3 0 49 122 38 661.3 0 38 123 60 661.3 0 60 124 37 661.3 0 37 125 58 661.3 0 58 126 64 661.3 0 64 127 68 661.3 0 68 128 112 661.3 0 112 129 134 661.3 0 134 130 64 661.3 0 64 131 43 661.3 0 43 132 59 661.3 0 59 133 127 661.3 0 127 134 171 661.3 0 171 135 168 661.3 0 168 136 104 661.3 0 104 137 97 661.3 0 97 138 239 661.3 0 239 139 203 661.3 0 203 140 434 661.3 0 434 141 815 661.3 0 815 142 743 661.3 0 743 143 176 661.3 0 176 144 103 661.3 0 103 145 140 661.3 0 140 146 79 661.3 0 79 147 112 661.3 0 112 148 1682 661.3 0 1682 149 857 661.3 0 857 150 1810 661.3 0 1810 151 61 661.3 0 61 152 53 661.3 0 53 153 60 661.3 0 60 154 60 661.3 0 60 155 264 661.3 0 264 156 136 661.3 0 136 157 147 661.3 0 147 158 96 661.3 0 96 159 35 661.3 0 35 160 37 661.3 0 37 161 77 661.3 0 77 162 67 661.3 0 67 163 249 661.3 0 249 164 93 661.3 0 93 165 225 661.3 0 225 166 58 661.3 0 58 167 53 661.3 0 53 168 80 661.3 0 80 169 36 661.3 0 36 170 39 661.3 0 39 171 24 661.3 0 24 172 25 661.3 0 25 173 42 661.3 0 42 174 41 661.3 0 41 175 43 661.3 0 43 176 31 661.3 0 31 177 48 661.3 0 48 178 120 661.3 0 120 179 49 661.3 0 49 180 115 661.3 0 115 181 154 661.3 0 154 182 111 661.3 0 111 183 79 661.3 0 79 184 85 661.3 0 85 185 75 661.3 0 75 186 54 661.3 0 54 187 48 661.3 0 48 188 35 661.3 0 35 189 68 661.3 0 68 190 70 661.3 0 70 191 78 661.3 0 78 192 72 661.3 0 72 193 68 661.3 0 68 194 62 661.3 0 62 195 68 661.3 0 68 196 54 661.3 0 54 197 48 661.3 0 48 198 45 661.3 0 45 199 35 661.3 0 35 200 76 661.3 0 76 201 46 661.3 0 46 202 76 661.3 0 76 203 138 661.3 0 138 204 105 661.3 0 105 205 84 661.3 0 84 206 49 661.3 0 49 207 62 661.3 0 62 208 41 661.3 0 41 209 60 661.3 0 60 210 56 661.3 0 56 211 44 661.3 0 44 212 51 661.3 0 51 213 69 661.3 0 69 214 79 661.3 0 79 215 106 661.3 0 106 216 106 661.3 0 106 217 108 661.3 0 108 218 61 661.3 0 61 219 55 661.3 0 55 220 57 661.3 0 57 221 79 661.3 0 79 222 47 661.3 0 47 223 65 661.3 0 65 224 77 661.3 0 77 225 74 661.3 0 74 226 79 661.3 0 79 227 89 661.3 0 89 228 76 661.3 0 76 229 66 661.3 0 66 230 45 661.3 0 45 231 82 661.3 0 82 232 140 661.3 0 140 233 144 661.3 0 144 234 112 661.3 0 112 235 123 661.3 0 123 236 141 661.3 0 141 237 90 661.3 0 90 238 61 661.3 0 61 239 59 661.3 0 59 240 46 661.3 0 46 241 57 661.3 0 57 242 67 661.3 0 67 243 83 661.3 0 83 244 106 661.3 0 106 245 64 661.3 0 64 246 62 661.3 0 62 247 55 661.3 0 55 248 61 661.3 0 61 249 62 661.3 0 62 250 57 661.3 0 57 251 47 661.3 0 47 252 42 661.3 0 42 253 143 661.3 0 143 254 187 661.3 0 187 255 142 661.3 0 142 256 121 661.3 0 121 257 114 661.3 0 114 258 98 661.3 0 98 259 136 661.3 0 136 260 130 661.3 0 130 261 77 661.3 0 77 262 96 661.3 0 96 263 88 661.3 0 88 264 87 661.3 0 87 265 83 661.3 0 83 266 71 661.3 0 71 267 67 661.3 0 67 268 75 661.3 0 75 269 266 661.3 0 266 270 115 661.3 0 115 271 136 661.3 0 136 272 114 661.3 0 114 273 162 661.3 0 162 274 100 661.3 0 100 275 82 661.3 0 82 276 136 661.3 0 136 277 106 661.3 0 106 278 100 661.3 0 100 279 96 661.3 0 96 280 131 661.3 0 131 281 159 661.3 0 159 282 63 661.3 0 63 283 78 661.3 0 78 284 119 661.3 0 119 285 93 661.3 0 93 286 71 661.3 0 71 287 53 661.3 0 53 288 78 661.3 0 78 289 97 661.3 0 97 290 68 661.3 0 68 291 85 661.3 0 85 292 110 661.3 0 110 293 104 661.3 0 104 294 195 661.3 0 195 295 429 661.3 0 429 296 270 661.3 0 270 297 224 661.3 0 224 298 76 661.3 0 76 299 37 661.3 0 37 300 44 661.3 0 44 301 18 661.3 0 18 Finished in 3.16 s (49 us/read; 1.21 M reads/minute). === Summary === Total reads processed: 63,885 Reads with adapters: 9,250 (14.5%) Reads that were too short: 5 (0.0%) Reads written (passing filters): 9,245 (14.5%) Total basepairs processed: 16,344,834 bp Total written (filtered): 2,492,189 bp (15.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 9250 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 14 0.0 3 0 0 0 1 13 18 4 0.0 3 0 0 0 1 3 19 90 0.0 3 0 89 1 20 8876 0.0 4 8765 108 3 21 32 0.0 4 10 19 0 2 1 22 7 0.0 4 3 0 2 1 1 23 2 0.0 4 0 0 0 1 1 27 1 0.0 4 1 35 1 0.0 4 0 0 0 0 1 44 1 0.0 4 0 0 0 0 1 72 1 0.0 4 0 0 0 0 1 126 6 0.0 4 1 0 0 0 5 127 192 0.0 4 0 0 0 0 192 128 6 0.0 4 0 0 0 0 6 134 1 0.0 4 0 0 0 0 1 135 12 0.0 4 0 0 0 0 12 153 2 0.0 4 0 0 0 0 2 232 1 0.0 4 0 0 0 0 1 265 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.31 s (34 us/read; 1.79 M reads/minute). === Summary === Total reads processed: 9,245 Reads with adapters: 1,864 (20.2%) Reads written (passing filters): 9,245 (100.0%) Total basepairs processed: 2,492,189 bp Total written (filtered): 2,056,815 bp (82.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1864 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 86.7% C: 3.5% G: 9.5% T: 0.2% none/other: 0.1% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "A" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 4 13 36.1 0 13 5 1 9.0 0 1 9 18 0.0 0 2 16 10 125 0.0 1 2 123 11 39 0.0 1 0 39 203 1 0.0 1 1 256 47 0.0 1 44 3 257 33 0.0 1 28 5 258 4 0.0 1 4 259 7 0.0 1 7 260 1476 0.0 1 1407 69 261 69 0.0 1 65 4 262 18 0.0 1 16 2 263 11 0.0 1 11 265 1 0.0 1 1 272 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.