This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATCCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 74.97 s (28 us/read; 2.17 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 1,098,048 (40.5%) Reads that were too short: 35,956 (1.3%) Reads written (passing filters): 1,062,092 (39.2%) Total basepairs processed: 815,312,680 bp Total written (filtered): 290,135,244 bp (35.6%) === Adapter 1 === Sequence: AATCCT; Type: regular 5'; Length: 6; Trimmed: 1098048 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 888 661.3 0 888 7 2016 661.3 0 2016 8 1492 661.3 0 1492 9 24675 661.3 0 24675 10 2356 661.3 0 2356 11 4103 661.3 0 4103 12 215664 661.3 0 215664 13 2428 661.3 0 2428 14 441 661.3 0 441 15 1121 661.3 0 1121 16 455 661.3 0 455 17 893 661.3 0 893 18 2077 661.3 0 2077 19 555 661.3 0 555 20 603 661.3 0 603 21 256 661.3 0 256 22 451 661.3 0 451 23 1050 661.3 0 1050 24 1814 661.3 0 1814 25 2732 661.3 0 2732 26 3998 661.3 0 3998 27 5726 661.3 0 5726 28 10345 661.3 0 10345 29 21722 661.3 0 21722 30 376739 661.3 0 376739 31 75381 661.3 0 75381 32 280749 661.3 0 280749 33 1537 661.3 0 1537 34 102 661.3 0 102 35 108 661.3 0 108 36 267 661.3 0 267 37 339 661.3 0 339 38 43 661.3 0 43 39 81 661.3 0 81 40 28 661.3 0 28 41 18 661.3 0 18 42 16 661.3 0 16 43 21 661.3 0 21 44 39 661.3 0 39 45 23 661.3 0 23 46 29 661.3 0 29 47 34 661.3 0 34 48 26 661.3 0 26 49 22 661.3 0 22 50 15 661.3 0 15 51 25 661.3 0 25 52 12 661.3 0 12 53 28 661.3 0 28 54 140 661.3 0 140 55 568 661.3 0 568 56 200 661.3 0 200 57 23 661.3 0 23 58 14 661.3 0 14 59 17 661.3 0 17 60 11 661.3 0 11 61 22 661.3 0 22 62 17 661.3 0 17 63 26 661.3 0 26 64 23 661.3 0 23 65 25 661.3 0 25 66 12 661.3 0 12 67 14 661.3 0 14 68 28 661.3 0 28 69 21 661.3 0 21 70 37 661.3 0 37 71 97 661.3 0 97 72 68 661.3 0 68 73 46 661.3 0 46 74 57 661.3 0 57 75 40 661.3 0 40 76 50 661.3 0 50 77 61 661.3 0 61 78 30 661.3 0 30 79 52 661.3 0 52 80 77 661.3 0 77 81 106 661.3 0 106 82 163 661.3 0 163 83 172 661.3 0 172 84 107 661.3 0 107 85 132 661.3 0 132 86 127 661.3 0 127 87 198 661.3 0 198 88 113 661.3 0 113 89 185 661.3 0 185 90 98 661.3 0 98 91 106 661.3 0 106 92 179 661.3 0 179 93 92 661.3 0 92 94 56 661.3 0 56 95 49 661.3 0 49 96 55 661.3 0 55 97 104 661.3 0 104 98 60 661.3 0 60 99 55 661.3 0 55 100 191 661.3 0 191 101 99 661.3 0 99 102 314 661.3 0 314 103 290 661.3 0 290 104 338 661.3 0 338 105 279 661.3 0 279 106 396 661.3 0 396 107 100 661.3 0 100 108 80 661.3 0 80 109 136 661.3 0 136 110 114 661.3 0 114 111 111 661.3 0 111 112 119 661.3 0 119 113 104 661.3 0 104 114 72 661.3 0 72 115 60 661.3 0 60 116 67 661.3 0 67 117 61 661.3 0 61 118 187 661.3 0 187 119 162 661.3 0 162 120 115 661.3 0 115 121 71 661.3 0 71 122 137 661.3 0 137 123 146 661.3 0 146 124 435 661.3 0 435 125 356 661.3 0 356 126 171 661.3 0 171 127 119 661.3 0 119 128 93 661.3 0 93 129 63 661.3 0 63 130 63 661.3 0 63 131 86 661.3 0 86 132 87 661.3 0 87 133 89 661.3 0 89 134 107 661.3 0 107 135 77 661.3 0 77 136 95 661.3 0 95 137 128 661.3 0 128 138 210 661.3 0 210 139 196 661.3 0 196 140 105 661.3 0 105 141 119 661.3 0 119 142 241 661.3 0 241 143 167 661.3 0 167 144 113 661.3 0 113 145 101 661.3 0 101 146 110 661.3 0 110 147 150 661.3 0 150 148 92 661.3 0 92 149 246 661.3 0 246 150 165 661.3 0 165 151 100 661.3 0 100 152 132 661.3 0 132 153 92 661.3 0 92 154 105 661.3 0 105 155 111 661.3 0 111 156 89 661.3 0 89 157 161 661.3 0 161 158 123 661.3 0 123 159 373 661.3 0 373 160 306 661.3 0 306 161 361 661.3 0 361 162 131 661.3 0 131 163 238 661.3 0 238 164 218 661.3 0 218 165 122 661.3 0 122 166 128 661.3 0 128 167 186 661.3 0 186 168 139 661.3 0 139 169 94 661.3 0 94 170 78 661.3 0 78 171 91 661.3 0 91 172 117 661.3 0 117 173 85 661.3 0 85 174 89 661.3 0 89 175 96 661.3 0 96 176 92 661.3 0 92 177 127 661.3 0 127 178 114 661.3 0 114 179 160 661.3 0 160 180 131 661.3 0 131 181 76 661.3 0 76 182 87 661.3 0 87 183 172 661.3 0 172 184 273 661.3 0 273 185 128 661.3 0 128 186 75 661.3 0 75 187 58 661.3 0 58 188 134 661.3 0 134 189 146 661.3 0 146 190 145 661.3 0 145 191 93 661.3 0 93 192 79 661.3 0 79 193 63 661.3 0 63 194 77 661.3 0 77 195 94 661.3 0 94 196 98 661.3 0 98 197 142 661.3 0 142 198 102 661.3 0 102 199 143 661.3 0 143 200 164 661.3 0 164 201 145 661.3 0 145 202 181 661.3 0 181 203 185 661.3 0 185 204 251 661.3 0 251 205 248 661.3 0 248 206 292 661.3 0 292 207 126 661.3 0 126 208 81 661.3 0 81 209 69 661.3 0 69 210 74 661.3 0 74 211 104 661.3 0 104 212 89 661.3 0 89 213 115 661.3 0 115 214 108 661.3 0 108 215 139 661.3 0 139 216 117 661.3 0 117 217 86 661.3 0 86 218 113 661.3 0 113 219 106 661.3 0 106 220 91 661.3 0 91 221 61 661.3 0 61 222 86 661.3 0 86 223 144 661.3 0 144 224 170 661.3 0 170 225 112 661.3 0 112 226 265 661.3 0 265 227 167 661.3 0 167 228 240 661.3 0 240 229 130 661.3 0 130 230 81 661.3 0 81 231 120 661.3 0 120 232 201 661.3 0 201 233 134 661.3 0 134 234 122 661.3 0 122 235 65 661.3 0 65 236 55 661.3 0 55 237 49 661.3 0 49 238 61 661.3 0 61 239 69 661.3 0 69 240 92 661.3 0 92 241 110 661.3 0 110 242 160 661.3 0 160 243 199 661.3 0 199 244 157 661.3 0 157 245 148 661.3 0 148 246 155 661.3 0 155 247 225 661.3 0 225 248 81 661.3 0 81 249 113 661.3 0 113 250 284 661.3 0 284 251 220 661.3 0 220 252 399 661.3 0 399 253 406 661.3 0 406 254 1142 661.3 0 1142 255 2096 661.3 0 2096 256 667 661.3 0 667 257 358 661.3 0 358 258 246 661.3 0 246 259 378 661.3 0 378 260 3136 661.3 0 3136 261 4065 661.3 0 4065 262 8580 661.3 0 8580 263 291 661.3 0 291 264 195 661.3 0 195 265 185 661.3 0 185 266 108 661.3 0 108 267 68 661.3 0 68 268 117 661.3 0 117 269 87 661.3 0 87 270 121 661.3 0 121 271 129 661.3 0 129 272 229 661.3 0 229 273 306 661.3 0 306 274 258 661.3 0 258 275 919 661.3 0 919 276 505 661.3 0 505 277 737 661.3 0 737 278 915 661.3 0 915 279 521 661.3 0 521 280 202 661.3 0 202 281 115 661.3 0 115 282 139 661.3 0 139 283 149 661.3 0 149 284 141 661.3 0 141 285 80 661.3 0 80 286 55 661.3 0 55 287 41 661.3 0 41 288 28 661.3 0 28 289 57 661.3 0 57 290 100 661.3 0 100 291 161 661.3 0 161 292 142 661.3 0 142 293 56 661.3 0 56 294 52 661.3 0 52 295 53 661.3 0 53 296 46 661.3 0 46 297 52 661.3 0 52 298 47 661.3 0 47 299 69 661.3 0 69 300 97 661.3 0 97 301 59 661.3 0 59 Finished in 43.53 s (41 us/read; 1.46 M reads/minute). === Summary === Total reads processed: 1,062,092 Reads with adapters: 27,115 (2.6%) Reads that were too short: 813 (0.1%) Reads written (passing filters): 26,302 (2.5%) Total basepairs processed: 290,135,244 bp Total written (filtered): 6,975,761 bp (2.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 27115 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 3 0.0 3 0 0 2 1 16 265 0.0 3 0 256 6 3 17 24285 0.0 3 23741 470 55 19 18 75 0.0 3 2 66 3 4 19 12 0.0 3 0 0 6 6 20 34 0.0 3 0 0 0 34 24 1 0.0 3 0 0 0 1 25 1 0.0 3 1 26 1 0.0 3 1 28 6 0.0 3 4 0 0 2 32 1 0.0 3 1 33 3 0.0 3 0 0 0 3 34 1 0.0 3 0 0 0 1 35 2 0.0 3 2 38 2 0.0 3 1 0 0 1 39 1 0.0 3 0 0 0 1 41 2 0.0 3 0 0 0 2 42 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 58 2 0.0 3 0 0 0 2 59 1 0.0 3 0 0 0 1 67 7 0.0 3 0 0 0 7 68 1 0.0 3 0 0 0 1 69 3 0.0 3 0 0 0 3 70 3 0.0 3 0 0 0 3 74 2 0.0 3 0 0 0 2 76 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 87 2 0.0 3 0 0 0 2 90 2 0.0 3 0 0 0 2 92 1 0.0 3 0 0 0 1 93 2 0.0 3 0 0 0 2 95 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 1 104 1 0.0 3 0 1 108 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 112 14 0.0 3 0 0 0 14 113 1 0.0 3 1 116 1 0.0 3 0 0 1 117 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 127 2 0.0 3 0 0 0 2 132 2 0.0 3 0 0 0 2 133 6 0.0 3 0 0 0 6 134 2 0.0 3 0 0 0 2 137 5 0.0 3 0 0 0 5 139 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 141 5 0.0 3 0 0 0 5 148 2 0.0 3 0 0 0 2 156 24 0.0 3 0 0 0 24 157 1 0.0 3 0 0 0 1 158 5 0.0 3 0 0 0 5 159 2 0.0 3 0 0 0 2 160 3 0.0 3 0 0 0 3 161 156 0.0 3 0 0 0 156 162 17 0.0 3 0 0 0 17 163 187 0.0 3 0 0 2 185 164 27 0.0 3 0 0 0 27 165 2 0.0 3 0 0 0 2 166 4 0.0 3 0 0 0 4 167 2 0.0 3 0 0 0 2 168 4 0.0 3 0 0 0 4 169 61 0.0 3 0 0 0 61 170 3 0.0 3 0 0 0 3 171 7 0.0 3 0 0 0 7 172 1 0.0 3 0 0 0 1 173 10 0.0 3 0 0 0 10 174 3 0.0 3 0 0 0 3 176 15 0.0 3 0 0 0 15 177 2 0.0 3 0 0 0 2 178 21 0.0 3 0 0 0 21 179 17 0.0 3 0 0 1 16 180 15 0.0 3 0 0 0 15 181 593 0.0 3 0 0 1 592 182 283 0.0 3 0 0 0 283 183 7 0.0 3 0 0 0 7 184 61 0.0 3 0 0 1 60 185 30 0.0 3 0 0 1 29 187 2 0.0 3 0 0 0 2 188 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 195 1 0.0 3 0 0 0 1 198 26 0.0 3 0 0 0 26 199 4 0.0 3 0 0 0 4 200 2 0.0 3 0 0 0 2 202 3 0.0 3 0 0 0 3 203 2 0.0 3 0 0 0 2 204 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 207 4 0.0 3 0 0 1 3 212 1 0.0 3 0 0 0 1 213 1 0.0 3 1 214 1 0.0 3 0 0 0 1 215 1 0.0 3 0 0 0 1 216 6 0.0 3 0 0 0 6 219 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 223 2 0.0 3 0 0 0 2 229 1 0.0 3 0 0 0 1 230 1 0.0 3 0 0 0 1 232 1 0.0 3 0 0 0 1 239 1 0.0 3 0 0 0 1 240 1 0.0 3 0 0 1 241 2 0.0 3 0 0 0 2 242 1 0.0 3 0 0 0 1 247 29 0.0 3 0 0 0 29 248 3 0.0 3 0 0 0 3 255 2 0.0 3 0 0 0 2 256 8 0.0 3 0 0 0 8 257 17 0.0 3 0 0 0 17 258 98 0.0 3 0 0 3 95 259 125 0.0 3 0 0 2 123 260 25 0.0 3 0 0 0 25 261 3 0.0 3 0 0 1 2 262 4 0.0 3 0 0 0 4 263 1 0.0 3 0 0 0 1 264 8 0.0 3 0 0 0 8 265 22 0.0 3 0 0 0 22 266 8 0.0 3 0 0 0 8 267 5 0.0 3 0 0 0 5 268 3 0.0 3 0 0 1 2 269 2 0.0 3 0 0 0 2 270 2 0.0 3 0 0 0 2 271 2 0.0 3 0 0 0 2 272 2 0.0 3 0 0 0 2 273 106 0.0 3 0 0 4 102 274 13 0.0 3 0 0 2 11 275 10 0.0 3 0 0 0 10 276 45 0.0 3 0 0 1 44 277 10 0.0 3 0 0 0 10 278 3 0.0 3 0 0 0 3 279 3 0.0 3 0 0 0 3 280 3 0.0 3 0 0 0 3 281 8 0.0 3 0 0 0 8 282 42 0.0 3 0 0 0 42 283 79 0.0 3 0 0 5 74 284 6 0.0 3 0 0 0 6 286 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 289 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.93 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 26,302 Reads with adapters: 1,775 (6.7%) Reads written (passing filters): 26,302 (100.0%) Total basepairs processed: 6,975,761 bp Total written (filtered): 6,970,421 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1775 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 34.5% C: 6.6% G: 56.2% T: 2.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1762 411.0 0 1762 4 12 102.7 0 12 6 1 6.4 0 1