This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTCGGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 70.48 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 400,447 (14.8%) Reads that were too short: 29,278 (1.1%) Reads written (passing filters): 371,169 (13.7%) Total basepairs processed: 815,312,680 bp Total written (filtered): 79,343,679 bp (9.7%) === Adapter 1 === Sequence: TTCGGA; Type: regular 5'; Length: 6; Trimmed: 400447 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 378 661.3 0 378 7 424 661.3 0 424 8 1475 661.3 0 1475 9 25482 661.3 0 25482 10 6371 661.3 0 6371 11 37 661.3 0 37 12 28 661.3 0 28 13 20 661.3 0 20 14 14 661.3 0 14 15 18 661.3 0 18 16 17 661.3 0 17 17 26 661.3 0 26 18 21 661.3 0 21 19 25 661.3 0 25 20 25 661.3 0 25 21 29 661.3 0 29 22 19 661.3 0 19 23 22 661.3 0 22 24 26 661.3 0 26 25 22 661.3 0 22 26 33 661.3 0 33 27 24 661.3 0 24 28 22 661.3 0 22 29 13 661.3 0 13 30 30 661.3 0 30 31 28 661.3 0 28 32 38 661.3 0 38 33 31 661.3 0 31 34 32 661.3 0 32 35 19 661.3 0 19 36 18 661.3 0 18 37 33 661.3 0 33 38 32 661.3 0 32 39 29 661.3 0 29 40 30 661.3 0 30 41 22 661.3 0 22 42 18 661.3 0 18 43 20 661.3 0 20 44 23 661.3 0 23 45 18 661.3 0 18 46 18 661.3 0 18 47 44 661.3 0 44 48 67 661.3 0 67 49 39 661.3 0 39 50 17 661.3 0 17 51 41 661.3 0 41 52 40 661.3 0 40 53 29 661.3 0 29 54 39 661.3 0 39 55 18 661.3 0 18 56 33 661.3 0 33 57 49 661.3 0 49 58 41 661.3 0 41 59 43 661.3 0 43 60 47 661.3 0 47 61 50 661.3 0 50 62 33 661.3 0 33 63 62 661.3 0 62 64 31 661.3 0 31 65 31 661.3 0 31 66 53 661.3 0 53 67 33 661.3 0 33 68 136 661.3 0 136 69 59 661.3 0 59 70 54 661.3 0 54 71 57 661.3 0 57 72 156 661.3 0 156 73 139 661.3 0 139 74 236 661.3 0 236 75 448 661.3 0 448 76 471 661.3 0 471 77 517 661.3 0 517 78 996 661.3 0 996 79 2620 661.3 0 2620 80 581 661.3 0 581 81 911 661.3 0 911 82 1874 661.3 0 1874 83 996 661.3 0 996 84 863 661.3 0 863 85 1788 661.3 0 1788 86 1973 661.3 0 1973 87 1906 661.3 0 1906 88 1197 661.3 0 1197 89 1537 661.3 0 1537 90 1772 661.3 0 1772 91 3167 661.3 0 3167 92 34718 661.3 0 34718 93 104756 661.3 0 104756 94 160020 661.3 0 160020 95 1090 661.3 0 1090 96 85 661.3 0 85 97 21 661.3 0 21 98 22 661.3 0 22 99 13 661.3 0 13 100 15 661.3 0 15 101 21 661.3 0 21 102 17 661.3 0 17 103 13 661.3 0 13 104 15 661.3 0 15 105 14 661.3 0 14 106 22 661.3 0 22 107 39 661.3 0 39 108 16 661.3 0 16 109 131 661.3 0 131 110 32 661.3 0 32 111 76 661.3 0 76 112 49 661.3 0 49 113 14 661.3 0 14 114 17 661.3 0 17 115 25 661.3 0 25 116 37 661.3 0 37 117 26 661.3 0 26 118 19 661.3 0 19 119 13 661.3 0 13 120 14 661.3 0 14 121 9 661.3 0 9 122 9 661.3 0 9 123 6 661.3 0 6 124 12 661.3 0 12 125 25 661.3 0 25 126 15 661.3 0 15 127 8 661.3 0 8 128 20 661.3 0 20 129 23 661.3 0 23 130 55 661.3 0 55 131 19 661.3 0 19 132 10 661.3 0 10 133 16 661.3 0 16 134 15 661.3 0 15 135 26 661.3 0 26 136 23 661.3 0 23 137 26 661.3 0 26 138 33 661.3 0 33 139 175 661.3 0 175 140 141 661.3 0 141 141 213 661.3 0 213 142 17 661.3 0 17 143 13 661.3 0 13 144 23 661.3 0 23 145 17 661.3 0 17 146 10 661.3 0 10 147 19 661.3 0 19 148 51 661.3 0 51 149 22 661.3 0 22 150 7 661.3 0 7 151 15 661.3 0 15 152 19 661.3 0 19 153 14 661.3 0 14 154 10 661.3 0 10 155 12 661.3 0 12 156 30 661.3 0 30 157 15 661.3 0 15 158 22 661.3 0 22 159 17 661.3 0 17 160 11 661.3 0 11 161 18 661.3 0 18 162 24 661.3 0 24 163 13 661.3 0 13 164 9 661.3 0 9 165 12 661.3 0 12 166 6 661.3 0 6 167 22 661.3 0 22 168 21 661.3 0 21 169 307 661.3 0 307 170 17 661.3 0 17 171 25 661.3 0 25 172 13 661.3 0 13 173 17 661.3 0 17 174 14 661.3 0 14 175 23 661.3 0 23 176 39 661.3 0 39 177 157 661.3 0 157 178 345 661.3 0 345 179 238 661.3 0 238 180 508 661.3 0 508 181 35 661.3 0 35 182 20 661.3 0 20 183 33 661.3 0 33 184 40 661.3 0 40 185 47 661.3 0 47 186 76 661.3 0 76 187 111 661.3 0 111 188 235 661.3 0 235 189 60 661.3 0 60 190 226 661.3 0 226 191 256 661.3 0 256 192 283 661.3 0 283 193 89 661.3 0 89 194 119 661.3 0 119 195 134 661.3 0 134 196 154 661.3 0 154 197 122 661.3 0 122 198 149 661.3 0 149 199 192 661.3 0 192 200 250 661.3 0 250 201 3833 661.3 0 3833 202 6351 661.3 0 6351 203 10308 661.3 0 10308 204 551 661.3 0 551 205 573 661.3 0 573 206 812 661.3 0 812 207 1532 661.3 0 1532 208 131 661.3 0 131 209 108 661.3 0 108 210 60 661.3 0 60 211 39 661.3 0 39 212 37 661.3 0 37 213 47 661.3 0 47 214 64 661.3 0 64 215 94 661.3 0 94 216 43 661.3 0 43 217 114 661.3 0 114 218 64 661.3 0 64 219 45 661.3 0 45 220 26 661.3 0 26 221 110 661.3 0 110 222 198 661.3 0 198 223 601 661.3 0 601 224 93 661.3 0 93 225 341 661.3 0 341 226 618 661.3 0 618 227 660 661.3 0 660 228 470 661.3 0 470 229 526 661.3 0 526 230 16 661.3 0 16 231 16 661.3 0 16 232 12 661.3 0 12 233 15 661.3 0 15 234 6 661.3 0 6 235 21 661.3 0 21 236 45 661.3 0 45 237 126 661.3 0 126 238 37 661.3 0 37 239 19 661.3 0 19 240 6 661.3 0 6 241 9 661.3 0 9 242 15 661.3 0 15 243 26 661.3 0 26 244 19 661.3 0 19 245 21 661.3 0 21 246 38 661.3 0 38 247 31 661.3 0 31 248 23 661.3 0 23 249 11 661.3 0 11 250 20 661.3 0 20 251 18 661.3 0 18 252 15 661.3 0 15 253 15 661.3 0 15 254 34 661.3 0 34 255 26 661.3 0 26 256 25 661.3 0 25 257 50 661.3 0 50 258 320 661.3 0 320 259 195 661.3 0 195 260 356 661.3 0 356 261 217 661.3 0 217 262 111 661.3 0 111 263 56 661.3 0 56 264 26 661.3 0 26 265 19 661.3 0 19 266 26 661.3 0 26 267 18 661.3 0 18 268 17 661.3 0 17 269 17 661.3 0 17 270 23 661.3 0 23 271 117 661.3 0 117 272 174 661.3 0 174 273 176 661.3 0 176 274 74 661.3 0 74 275 21 661.3 0 21 276 42 661.3 0 42 277 25 661.3 0 25 278 34 661.3 0 34 279 43 661.3 0 43 280 38 661.3 0 38 281 40 661.3 0 40 282 36 661.3 0 36 283 62 661.3 0 62 284 94 661.3 0 94 285 53 661.3 0 53 286 494 661.3 0 494 287 772 661.3 0 772 288 21 661.3 0 21 289 6 661.3 0 6 290 7 661.3 0 7 291 15 661.3 0 15 292 17 661.3 0 17 293 29 661.3 0 29 294 33 661.3 0 33 295 13 661.3 0 13 296 22 661.3 0 22 297 16 661.3 0 16 298 16 661.3 0 16 299 29 661.3 0 29 300 8 661.3 0 8 301 19 661.3 0 19 Finished in 15.13 s (41 us/read; 1.47 M reads/minute). === Summary === Total reads processed: 371,169 Reads with adapters: 25,926 (7.0%) Reads that were too short: 17 (0.0%) Reads written (passing filters): 25,909 (7.0%) Total basepairs processed: 79,343,679 bp Total written (filtered): 7,047,261 bp (8.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 25926 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 11 0.0 3 0 0 0 0 11 18 12 0.0 3 0 0 11 1 19 507 0.0 3 0 499 7 0 1 20 25273 0.0 4 24930 325 13 1 4 21 63 0.0 4 15 48 22 2 0.0 4 0 0 2 23 18 0.0 4 0 0 0 16 2 24 2 0.0 4 1 0 0 0 1 27 1 0.0 4 1 44 1 0.0 4 1 67 2 0.0 4 0 0 0 0 2 68 14 0.0 4 0 0 0 0 14 70 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 0 1 96 1 0.0 4 0 0 0 0 1 123 1 0.0 4 0 0 0 0 1 128 2 0.0 4 0 0 0 0 2 131 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 150 2 0.0 4 0 0 0 0 2 151 1 0.0 4 0 0 0 0 1 171 1 0.0 4 0 0 0 0 1 173 1 0.0 4 0 0 0 0 1 177 1 0.0 4 0 0 0 1 178 1 0.0 4 0 0 0 0 1 198 3 0.0 4 0 0 0 0 3 254 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.88 s (34 us/read; 1.77 M reads/minute). === Summary === Total reads processed: 25,909 Reads with adapters: 2,258 (8.7%) Reads written (passing filters): 25,909 (100.0%) Total basepairs processed: 7,047,261 bp Total written (filtered): 6,639,864 bp (94.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2258 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 66.9% C: 3.3% G: 29.1% T: 0.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 4 2 101.2 0 2 6 3 6.3 0 3 9 99 0.1 0 6 93 10 314 0.0 1 16 298 11 269 0.0 1 0 269 12 3 0.0 1 0 3 13 1 0.0 1 0 1 223 51 0.0 1 49 2 224 5 0.0 1 5 225 2 0.0 1 2 226 1 0.0 1 1 228 19 0.0 1 17 2 234 1 0.0 1 1 256 4 0.0 1 3 1 257 1356 0.0 1 1254 102 258 97 0.0 1 93 4 259 15 0.0 1 15 260 15 0.0 1 11 4 270 1 0.0 1 1