This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TAATGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 70.18 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 200,318 (7.4%) Reads that were too short: 67,493 (2.5%) Reads written (passing filters): 132,825 (4.9%) Total basepairs processed: 815,312,680 bp Total written (filtered): 24,745,191 bp (3.0%) === Adapter 1 === Sequence: TAATGA; Type: regular 5'; Length: 6; Trimmed: 200318 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 168 661.3 0 168 7 6354 661.3 0 6354 8 6009 661.3 0 6009 9 50 661.3 0 50 10 33 661.3 0 33 11 44 661.3 0 44 12 45 661.3 0 45 13 39 661.3 0 39 14 30 661.3 0 30 15 32 661.3 0 32 16 30 661.3 0 30 17 45 661.3 0 45 18 32 661.3 0 32 19 38 661.3 0 38 20 40 661.3 0 40 21 35 661.3 0 35 22 27 661.3 0 27 23 56 661.3 0 56 24 37 661.3 0 37 25 49 661.3 0 49 26 51 661.3 0 51 27 98 661.3 0 98 28 123 661.3 0 123 29 2108 661.3 0 2108 30 271 661.3 0 271 31 74 661.3 0 74 32 47 661.3 0 47 33 30 661.3 0 30 34 59 661.3 0 59 35 47 661.3 0 47 36 49 661.3 0 49 37 57 661.3 0 57 38 39 661.3 0 39 39 49 661.3 0 49 40 37 661.3 0 37 41 30 661.3 0 30 42 56 661.3 0 56 43 56 661.3 0 56 44 40 661.3 0 40 45 31 661.3 0 31 46 55 661.3 0 55 47 37 661.3 0 37 48 35 661.3 0 35 49 47 661.3 0 47 50 42 661.3 0 42 51 31 661.3 0 31 52 48 661.3 0 48 53 50 661.3 0 50 54 44 661.3 0 44 55 42 661.3 0 42 56 36 661.3 0 36 57 66 661.3 0 66 58 45 661.3 0 45 59 41 661.3 0 41 60 51 661.3 0 51 61 60 661.3 0 60 62 48 661.3 0 48 63 38 661.3 0 38 64 38 661.3 0 38 65 44 661.3 0 44 66 56 661.3 0 56 67 62 661.3 0 62 68 82 661.3 0 82 69 107 661.3 0 107 70 1253 661.3 0 1253 71 661 661.3 0 661 72 1068 661.3 0 1068 73 109 661.3 0 109 74 63 661.3 0 63 75 88 661.3 0 88 76 357 661.3 0 357 77 145 661.3 0 145 78 341 661.3 0 341 79 524 661.3 0 524 80 252 661.3 0 252 81 868 661.3 0 868 82 859 661.3 0 859 83 701 661.3 0 701 84 346 661.3 0 346 85 556 661.3 0 556 86 262 661.3 0 262 87 215 661.3 0 215 88 156 661.3 0 156 89 242 661.3 0 242 90 195 661.3 0 195 91 272 661.3 0 272 92 371 661.3 0 371 93 400 661.3 0 400 94 362 661.3 0 362 95 260 661.3 0 260 96 279 661.3 0 279 97 224 661.3 0 224 98 343 661.3 0 343 99 954 661.3 0 954 100 511 661.3 0 511 101 188 661.3 0 188 102 368 661.3 0 368 103 251 661.3 0 251 104 128 661.3 0 128 105 87 661.3 0 87 106 147 661.3 0 147 107 578 661.3 0 578 108 364 661.3 0 364 109 323 661.3 0 323 110 405 661.3 0 405 111 824 661.3 0 824 112 393 661.3 0 393 113 499 661.3 0 499 114 759 661.3 0 759 115 721 661.3 0 721 116 675 661.3 0 675 117 638 661.3 0 638 118 588 661.3 0 588 119 661 661.3 0 661 120 583 661.3 0 583 121 583 661.3 0 583 122 658 661.3 0 658 123 856 661.3 0 856 124 8415 661.3 0 8415 125 25828 661.3 0 25828 126 22261 661.3 0 22261 127 615 661.3 0 615 128 473 661.3 0 473 129 684 661.3 0 684 130 584 661.3 0 584 131 477 661.3 0 477 132 300 661.3 0 300 133 390 661.3 0 390 134 332 661.3 0 332 135 410 661.3 0 410 136 445 661.3 0 445 137 398 661.3 0 398 138 351 661.3 0 351 139 560 661.3 0 560 140 668 661.3 0 668 141 419 661.3 0 419 142 347 661.3 0 347 143 306 661.3 0 306 144 400 661.3 0 400 145 374 661.3 0 374 146 395 661.3 0 395 147 339 661.3 0 339 148 430 661.3 0 430 149 1116 661.3 0 1116 150 1506 661.3 0 1506 151 2818 661.3 0 2818 152 607 661.3 0 607 153 320 661.3 0 320 154 360 661.3 0 360 155 339 661.3 0 339 156 447 661.3 0 447 157 402 661.3 0 402 158 349 661.3 0 349 159 369 661.3 0 369 160 304 661.3 0 304 161 334 661.3 0 334 162 363 661.3 0 363 163 422 661.3 0 422 164 489 661.3 0 489 165 611 661.3 0 611 166 399 661.3 0 399 167 329 661.3 0 329 168 291 661.3 0 291 169 462 661.3 0 462 170 351 661.3 0 351 171 289 661.3 0 289 172 265 661.3 0 265 173 316 661.3 0 316 174 237 661.3 0 237 175 264 661.3 0 264 176 312 661.3 0 312 177 370 661.3 0 370 178 390 661.3 0 390 179 356 661.3 0 356 180 299 661.3 0 299 181 503 661.3 0 503 182 476 661.3 0 476 183 437 661.3 0 437 184 374 661.3 0 374 185 450 661.3 0 450 186 387 661.3 0 387 187 270 661.3 0 270 188 379 661.3 0 379 189 380 661.3 0 380 190 438 661.3 0 438 191 372 661.3 0 372 192 346 661.3 0 346 193 382 661.3 0 382 194 260 661.3 0 260 195 301 661.3 0 301 196 568 661.3 0 568 197 652 661.3 0 652 198 472 661.3 0 472 199 251 661.3 0 251 200 180 661.3 0 180 201 396 661.3 0 396 202 502 661.3 0 502 203 432 661.3 0 432 204 382 661.3 0 382 205 485 661.3 0 485 206 469 661.3 0 469 207 473 661.3 0 473 208 459 661.3 0 459 209 537 661.3 0 537 210 464 661.3 0 464 211 444 661.3 0 444 212 457 661.3 0 457 213 443 661.3 0 443 214 459 661.3 0 459 215 463 661.3 0 463 216 451 661.3 0 451 217 448 661.3 0 448 218 468 661.3 0 468 219 400 661.3 0 400 220 416 661.3 0 416 221 484 661.3 0 484 222 651 661.3 0 651 223 967 661.3 0 967 224 1193 661.3 0 1193 225 9025 661.3 0 9025 226 2770 661.3 0 2770 227 11345 661.3 0 11345 228 429 661.3 0 429 229 289 661.3 0 289 230 371 661.3 0 371 231 375 661.3 0 375 232 402 661.3 0 402 233 456 661.3 0 456 234 677 661.3 0 677 235 762 661.3 0 762 236 455 661.3 0 455 237 509 661.3 0 509 238 554 661.3 0 554 239 388 661.3 0 388 240 327 661.3 0 327 241 446 661.3 0 446 242 527 661.3 0 527 243 408 661.3 0 408 244 409 661.3 0 409 245 485 661.3 0 485 246 452 661.3 0 452 247 354 661.3 0 354 248 259 661.3 0 259 249 229 661.3 0 229 250 350 661.3 0 350 251 561 661.3 0 561 252 437 661.3 0 437 253 266 661.3 0 266 254 312 661.3 0 312 255 335 661.3 0 335 256 441 661.3 0 441 257 430 661.3 0 430 258 317 661.3 0 317 259 314 661.3 0 314 260 455 661.3 0 455 261 517 661.3 0 517 262 545 661.3 0 545 263 441 661.3 0 441 264 394 661.3 0 394 265 314 661.3 0 314 266 461 661.3 0 461 267 463 661.3 0 463 268 374 661.3 0 374 269 299 661.3 0 299 270 546 661.3 0 546 271 560 661.3 0 560 272 486 661.3 0 486 273 658 661.3 0 658 274 682 661.3 0 682 275 268 661.3 0 268 276 337 661.3 0 337 277 626 661.3 0 626 278 455 661.3 0 455 279 322 661.3 0 322 280 290 661.3 0 290 281 298 661.3 0 298 282 378 661.3 0 378 283 440 661.3 0 440 284 404 661.3 0 404 285 540 661.3 0 540 286 343 661.3 0 343 287 209 661.3 0 209 288 386 661.3 0 386 289 424 661.3 0 424 290 463 661.3 0 463 291 571 661.3 0 571 292 392 661.3 0 392 293 383 661.3 0 383 294 515 661.3 0 515 295 531 661.3 0 531 296 659 661.3 0 659 297 651 661.3 0 651 298 685 661.3 0 685 299 600 661.3 0 600 300 329 661.3 0 329 301 386 661.3 0 386 Finished in 4.57 s (34 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 132,825 Reads with adapters: 6,603 (5.0%) Reads that were too short: 76 (0.1%) Reads written (passing filters): 6,527 (4.9%) Total basepairs processed: 24,745,191 bp Total written (filtered): 1,757,957 bp (7.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 6603 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 68 0.0 3 0 65 0 3 17 4087 0.0 3 3982 85 10 10 18 2047 0.0 3 1987 48 8 4 19 14 0.0 3 0 6 5 3 20 6 0.0 3 1 0 2 3 21 3 0.0 3 0 0 0 3 26 9 0.0 3 0 0 0 9 29 4 0.0 3 0 0 0 4 32 1 0.0 3 0 0 0 1 34 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 42 95 0.0 3 0 0 0 95 45 17 0.0 3 0 0 0 17 48 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 51 2 0.0 3 0 0 0 2 55 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 58 2 0.0 3 0 0 0 2 60 2 0.0 3 0 0 0 2 65 3 0.0 3 0 0 0 3 66 4 0.0 3 0 0 0 4 68 137 0.0 3 0 0 0 137 69 8 0.0 3 0 0 0 8 70 2 0.0 3 0 0 0 2 74 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 78 2 0.0 3 0 0 0 2 81 1 0.0 3 0 0 0 1 83 2 0.0 3 0 0 0 2 84 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 93 2 0.0 3 0 0 0 2 95 1 0.0 3 0 0 0 1 102 4 0.0 3 0 0 0 4 108 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 113 2 0.0 3 0 0 0 2 115 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 121 11 0.0 3 0 0 0 11 126 2 0.0 3 0 0 0 2 127 5 0.0 3 0 0 0 5 128 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 143 3 0.0 3 0 0 0 3 144 19 0.0 3 0 0 0 19 145 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 1 155 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 223 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.23 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 6,527 Reads with adapters: 373 (5.7%) Reads written (passing filters): 6,527 (100.0%) Total basepairs processed: 1,757,957 bp Total written (filtered): 1,756,831 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 373 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 35.4% C: 36.5% G: 21.2% T: 7.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 368 102.0 0 368 4 4 25.5 0 4 6 1 1.6 0 1