This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTTCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 68.04 s (25 us/read; 2.39 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 30,434 (1.1%) Reads that were too short: 6,616 (0.2%) Reads written (passing filters): 23,818 (0.9%) Total basepairs processed: 815,312,680 bp Total written (filtered): 5,377,666 bp (0.7%) === Adapter 1 === Sequence: GGTTCT; Type: regular 5'; Length: 6; Trimmed: 30434 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 73 661.3 0 73 7 124 661.3 0 124 8 144 661.3 0 144 9 5571 661.3 0 5571 10 138 661.3 0 138 11 49 661.3 0 49 12 43 661.3 0 43 13 48 661.3 0 48 14 26 661.3 0 26 15 53 661.3 0 53 16 70 661.3 0 70 17 51 661.3 0 51 18 52 661.3 0 52 19 62 661.3 0 62 20 67 661.3 0 67 21 62 661.3 0 62 22 102 661.3 0 102 23 727 661.3 0 727 24 191 661.3 0 191 25 568 661.3 0 568 26 50 661.3 0 50 27 41 661.3 0 41 28 35 661.3 0 35 29 64 661.3 0 64 30 45 661.3 0 45 31 36 661.3 0 36 32 48 661.3 0 48 33 42 661.3 0 42 34 48 661.3 0 48 35 73 661.3 0 73 36 49 661.3 0 49 37 60 661.3 0 60 38 44 661.3 0 44 39 42 661.3 0 42 40 46 661.3 0 46 41 46 661.3 0 46 42 34 661.3 0 34 43 43 661.3 0 43 44 53 661.3 0 53 45 52 661.3 0 52 46 46 661.3 0 46 47 51 661.3 0 51 48 38 661.3 0 38 49 50 661.3 0 50 50 42 661.3 0 42 51 36 661.3 0 36 52 41 661.3 0 41 53 30 661.3 0 30 54 54 661.3 0 54 55 56 661.3 0 56 56 43 661.3 0 43 57 41 661.3 0 41 58 46 661.3 0 46 59 47 661.3 0 47 60 36 661.3 0 36 61 47 661.3 0 47 62 33 661.3 0 33 63 43 661.3 0 43 64 50 661.3 0 50 65 48 661.3 0 48 66 44 661.3 0 44 67 33 661.3 0 33 68 34 661.3 0 34 69 49 661.3 0 49 70 42 661.3 0 42 71 56 661.3 0 56 72 49 661.3 0 49 73 46 661.3 0 46 74 75 661.3 0 75 75 62 661.3 0 62 76 39 661.3 0 39 77 54 661.3 0 54 78 48 661.3 0 48 79 40 661.3 0 40 80 59 661.3 0 59 81 57 661.3 0 57 82 92 661.3 0 92 83 73 661.3 0 73 84 76 661.3 0 76 85 68 661.3 0 68 86 102 661.3 0 102 87 75 661.3 0 75 88 493 661.3 0 493 89 1303 661.3 0 1303 90 1579 661.3 0 1579 91 30 661.3 0 30 92 35 661.3 0 35 93 37 661.3 0 37 94 48 661.3 0 48 95 55 661.3 0 55 96 194 661.3 0 194 97 117 661.3 0 117 98 56 661.3 0 56 99 52 661.3 0 52 100 84 661.3 0 84 101 398 661.3 0 398 102 131 661.3 0 131 103 253 661.3 0 253 104 19 661.3 0 19 105 49 661.3 0 49 106 38 661.3 0 38 107 24 661.3 0 24 108 26 661.3 0 26 109 37 661.3 0 37 110 55 661.3 0 55 111 66 661.3 0 66 112 55 661.3 0 55 113 78 661.3 0 78 114 70 661.3 0 70 115 72 661.3 0 72 116 34 661.3 0 34 117 78 661.3 0 78 118 61 661.3 0 61 119 60 661.3 0 60 120 48 661.3 0 48 121 58 661.3 0 58 122 52 661.3 0 52 123 66 661.3 0 66 124 54 661.3 0 54 125 54 661.3 0 54 126 76 661.3 0 76 127 1582 661.3 0 1582 128 753 661.3 0 753 129 1249 661.3 0 1249 130 73 661.3 0 73 131 48 661.3 0 48 132 30 661.3 0 30 133 46 661.3 0 46 134 65 661.3 0 65 135 75 661.3 0 75 136 33 661.3 0 33 137 29 661.3 0 29 138 34 661.3 0 34 139 46 661.3 0 46 140 52 661.3 0 52 141 189 661.3 0 189 142 44 661.3 0 44 143 28 661.3 0 28 144 29 661.3 0 29 145 52 661.3 0 52 146 50 661.3 0 50 147 40 661.3 0 40 148 29 661.3 0 29 149 45 661.3 0 45 150 31 661.3 0 31 151 36 661.3 0 36 152 29 661.3 0 29 153 34 661.3 0 34 154 32 661.3 0 32 155 37 661.3 0 37 156 41 661.3 0 41 157 31 661.3 0 31 158 30 661.3 0 30 159 37 661.3 0 37 160 32 661.3 0 32 161 38 661.3 0 38 162 27 661.3 0 27 163 24 661.3 0 24 164 34 661.3 0 34 165 28 661.3 0 28 166 26 661.3 0 26 167 50 661.3 0 50 168 44 661.3 0 44 169 49 661.3 0 49 170 39 661.3 0 39 171 32 661.3 0 32 172 31 661.3 0 31 173 23 661.3 0 23 174 23 661.3 0 23 175 31 661.3 0 31 176 54 661.3 0 54 177 26 661.3 0 26 178 32 661.3 0 32 179 33 661.3 0 33 180 22 661.3 0 22 181 24 661.3 0 24 182 24 661.3 0 24 183 24 661.3 0 24 184 21 661.3 0 21 185 36 661.3 0 36 186 35 661.3 0 35 187 40 661.3 0 40 188 28 661.3 0 28 189 17 661.3 0 17 190 29 661.3 0 29 191 29 661.3 0 29 192 26 661.3 0 26 193 53 661.3 0 53 194 50 661.3 0 50 195 48 661.3 0 48 196 45 661.3 0 45 197 44 661.3 0 44 198 31 661.3 0 31 199 20 661.3 0 20 200 57 661.3 0 57 201 62 661.3 0 62 202 99 661.3 0 99 203 46 661.3 0 46 204 35 661.3 0 35 205 32 661.3 0 32 206 38 661.3 0 38 207 48 661.3 0 48 208 49 661.3 0 49 209 36 661.3 0 36 210 44 661.3 0 44 211 41 661.3 0 41 212 26 661.3 0 26 213 38 661.3 0 38 214 47 661.3 0 47 215 60 661.3 0 60 216 47 661.3 0 47 217 44 661.3 0 44 218 45 661.3 0 45 219 71 661.3 0 71 220 35 661.3 0 35 221 37 661.3 0 37 222 37 661.3 0 37 223 44 661.3 0 44 224 52 661.3 0 52 225 41 661.3 0 41 226 59 661.3 0 59 227 44 661.3 0 44 228 23 661.3 0 23 229 38 661.3 0 38 230 43 661.3 0 43 231 31 661.3 0 31 232 35 661.3 0 35 233 35 661.3 0 35 234 71 661.3 0 71 235 264 661.3 0 264 236 45 661.3 0 45 237 31 661.3 0 31 238 31 661.3 0 31 239 49 661.3 0 49 240 39 661.3 0 39 241 58 661.3 0 58 242 38 661.3 0 38 243 51 661.3 0 51 244 46 661.3 0 46 245 40 661.3 0 40 246 35 661.3 0 35 247 56 661.3 0 56 248 37 661.3 0 37 249 50 661.3 0 50 250 38 661.3 0 38 251 32 661.3 0 32 252 33 661.3 0 33 253 47 661.3 0 47 254 84 661.3 0 84 255 126 661.3 0 126 256 89 661.3 0 89 257 52 661.3 0 52 258 50 661.3 0 50 259 119 661.3 0 119 260 304 661.3 0 304 261 612 661.3 0 612 262 1378 661.3 0 1378 263 33 661.3 0 33 264 30 661.3 0 30 265 26 661.3 0 26 266 33 661.3 0 33 267 26 661.3 0 26 268 31 661.3 0 31 269 26 661.3 0 26 270 13 661.3 0 13 271 16 661.3 0 16 272 29 661.3 0 29 273 29 661.3 0 29 274 35 661.3 0 35 275 24 661.3 0 24 276 37 661.3 0 37 277 30 661.3 0 30 278 24 661.3 0 24 279 149 661.3 0 149 280 102 661.3 0 102 281 37 661.3 0 37 282 31 661.3 0 31 283 53 661.3 0 53 284 26 661.3 0 26 285 23 661.3 0 23 286 35 661.3 0 35 287 38 661.3 0 38 288 23 661.3 0 23 289 16 661.3 0 16 290 15 661.3 0 15 291 23 661.3 0 23 292 21 661.3 0 21 293 36 661.3 0 36 294 26 661.3 0 26 295 34 661.3 0 34 296 19 661.3 0 19 297 51 661.3 0 51 298 30 661.3 0 30 299 24 661.3 0 24 300 25 661.3 0 25 301 32 661.3 0 32 Finished in 0.99 s (42 us/read; 1.44 M reads/minute). === Summary === Total reads processed: 23,818 Reads with adapters: 5,797 (24.3%) Reads that were too short: 20 (0.1%) Reads written (passing filters): 5,777 (24.3%) Total basepairs processed: 5,377,666 bp Total written (filtered): 1,574,750 bp (29.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 5797 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 7 0.0 3 0 0 6 1 16 72 0.0 3 0 68 2 2 17 5558 0.0 3 5430 108 15 5 18 23 0.0 3 2 12 9 19 19 0.0 3 1 0 7 11 20 8 0.0 3 1 0 0 7 25 1 0.0 3 0 0 0 1 40 6 0.0 3 0 0 4 2 62 1 0.0 3 0 0 0 1 64 32 0.0 3 0 0 0 32 66 2 0.0 3 0 0 0 2 67 9 0.0 3 0 0 0 9 69 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 96 3 0.0 3 0 0 0 3 97 1 0.0 3 0 0 0 1 103 25 0.0 3 0 0 0 25 104 1 0.0 3 0 0 0 1 106 3 0.0 3 0 0 0 3 108 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 166 9 0.0 3 0 0 0 9 167 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 204 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 264 1 0.0 3 0 0 0 1 271 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.21 s (36 us/read; 1.65 M reads/minute). === Summary === Total reads processed: 5,777 Reads with adapters: 260 (4.5%) Reads written (passing filters): 5,777 (100.0%) Total basepairs processed: 1,574,750 bp Total written (filtered): 1,573,966 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 260 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 42.7% C: 6.5% G: 45.8% T: 5.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 256 90.3 0 256 4 4 22.6 0 4