This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGAATC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 70.10 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 96,819 (3.6%) Reads that were too short: 9,607 (0.4%) Reads written (passing filters): 87,212 (3.2%) Total basepairs processed: 815,312,680 bp Total written (filtered): 22,909,641 bp (2.8%) === Adapter 1 === Sequence: CGAATC; Type: regular 5'; Length: 6; Trimmed: 96819 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 461 661.3 0 461 7 7342 661.3 0 7342 8 821 661.3 0 821 9 41411 661.3 0 41411 10 65 661.3 0 65 11 28 661.3 0 28 12 20 661.3 0 20 13 25 661.3 0 25 14 21 661.3 0 21 15 26 661.3 0 26 16 32 661.3 0 32 17 18 661.3 0 18 18 26 661.3 0 26 19 23 661.3 0 23 20 19 661.3 0 19 21 25 661.3 0 25 22 22 661.3 0 22 23 42 661.3 0 42 24 44 661.3 0 44 25 34 661.3 0 34 26 26 661.3 0 26 27 53 661.3 0 53 28 75 661.3 0 75 29 40 661.3 0 40 30 31 661.3 0 31 31 24 661.3 0 24 32 18 661.3 0 18 33 60 661.3 0 60 34 73 661.3 0 73 35 106 661.3 0 106 36 51 661.3 0 51 37 99 661.3 0 99 38 61 661.3 0 61 39 943 661.3 0 943 40 37 661.3 0 37 41 32 661.3 0 32 42 62 661.3 0 62 43 54 661.3 0 54 44 44 661.3 0 44 45 43 661.3 0 43 46 39 661.3 0 39 47 35 661.3 0 35 48 108 661.3 0 108 49 92 661.3 0 92 50 146 661.3 0 146 51 173 661.3 0 173 52 277 661.3 0 277 53 688 661.3 0 688 54 9171 661.3 0 9171 55 2300 661.3 0 2300 56 10889 661.3 0 10889 57 68 661.3 0 68 58 27 661.3 0 27 59 16 661.3 0 16 60 27 661.3 0 27 61 21 661.3 0 21 62 22 661.3 0 22 63 16 661.3 0 16 64 17 661.3 0 17 65 102 661.3 0 102 66 12 661.3 0 12 67 17 661.3 0 17 68 52 661.3 0 52 69 44 661.3 0 44 70 18 661.3 0 18 71 11 661.3 0 11 72 14 661.3 0 14 73 21 661.3 0 21 74 12 661.3 0 12 75 17 661.3 0 17 76 25 661.3 0 25 77 14 661.3 0 14 78 18 661.3 0 18 79 10 661.3 0 10 80 12 661.3 0 12 81 31 661.3 0 31 82 24 661.3 0 24 83 24 661.3 0 24 84 41 661.3 0 41 85 21 661.3 0 21 86 17 661.3 0 17 87 35 661.3 0 35 88 52 661.3 0 52 89 48 661.3 0 48 90 37 661.3 0 37 91 29 661.3 0 29 92 28 661.3 0 28 93 46 661.3 0 46 94 208 661.3 0 208 95 270 661.3 0 270 96 417 661.3 0 417 97 37 661.3 0 37 98 65 661.3 0 65 99 31 661.3 0 31 100 26 661.3 0 26 101 39 661.3 0 39 102 42 661.3 0 42 103 20 661.3 0 20 104 31 661.3 0 31 105 32 661.3 0 32 106 23 661.3 0 23 107 31 661.3 0 31 108 23 661.3 0 23 109 37 661.3 0 37 110 117 661.3 0 117 111 50 661.3 0 50 112 26 661.3 0 26 113 22 661.3 0 22 114 28 661.3 0 28 115 36 661.3 0 36 116 41 661.3 0 41 117 61 661.3 0 61 118 56 661.3 0 56 119 122 661.3 0 122 120 103 661.3 0 103 121 484 661.3 0 484 122 381 661.3 0 381 123 493 661.3 0 493 124 47 661.3 0 47 125 37 661.3 0 37 126 56 661.3 0 56 127 51 661.3 0 51 128 50 661.3 0 50 129 93 661.3 0 93 130 50 661.3 0 50 131 49 661.3 0 49 132 51 661.3 0 51 133 49 661.3 0 49 134 40 661.3 0 40 135 61 661.3 0 61 136 53 661.3 0 53 137 57 661.3 0 57 138 54 661.3 0 54 139 64 661.3 0 64 140 104 661.3 0 104 141 82 661.3 0 82 142 54 661.3 0 54 143 82 661.3 0 82 144 48 661.3 0 48 145 99 661.3 0 99 146 86 661.3 0 86 147 94 661.3 0 94 148 92 661.3 0 92 149 108 661.3 0 108 150 60 661.3 0 60 151 98 661.3 0 98 152 64 661.3 0 64 153 80 661.3 0 80 154 60 661.3 0 60 155 67 661.3 0 67 156 88 661.3 0 88 157 91 661.3 0 91 158 86 661.3 0 86 159 243 661.3 0 243 160 127 661.3 0 127 161 106 661.3 0 106 162 100 661.3 0 100 163 66 661.3 0 66 164 53 661.3 0 53 165 84 661.3 0 84 166 103 661.3 0 103 167 122 661.3 0 122 168 97 661.3 0 97 169 56 661.3 0 56 170 67 661.3 0 67 171 68 661.3 0 68 172 61 661.3 0 61 173 73 661.3 0 73 174 77 661.3 0 77 175 148 661.3 0 148 176 349 661.3 0 349 177 175 661.3 0 175 178 169 661.3 0 169 179 48 661.3 0 48 180 79 661.3 0 79 181 97 661.3 0 97 182 78 661.3 0 78 183 82 661.3 0 82 184 54 661.3 0 54 185 69 661.3 0 69 186 72 661.3 0 72 187 80 661.3 0 80 188 104 661.3 0 104 189 69 661.3 0 69 190 41 661.3 0 41 191 61 661.3 0 61 192 74 661.3 0 74 193 86 661.3 0 86 194 88 661.3 0 88 195 115 661.3 0 115 196 89 661.3 0 89 197 44 661.3 0 44 198 44 661.3 0 44 199 54 661.3 0 54 200 75 661.3 0 75 201 107 661.3 0 107 202 44 661.3 0 44 203 41 661.3 0 41 204 71 661.3 0 71 205 82 661.3 0 82 206 72 661.3 0 72 207 74 661.3 0 74 208 73 661.3 0 73 209 62 661.3 0 62 210 56 661.3 0 56 211 68 661.3 0 68 212 57 661.3 0 57 213 78 661.3 0 78 214 83 661.3 0 83 215 72 661.3 0 72 216 63 661.3 0 63 217 65 661.3 0 65 218 66 661.3 0 66 219 91 661.3 0 91 220 139 661.3 0 139 221 154 661.3 0 154 222 106 661.3 0 106 223 77 661.3 0 77 224 63 661.3 0 63 225 90 661.3 0 90 226 87 661.3 0 87 227 77 661.3 0 77 228 77 661.3 0 77 229 84 661.3 0 84 230 116 661.3 0 116 231 72 661.3 0 72 232 103 661.3 0 103 233 126 661.3 0 126 234 70 661.3 0 70 235 46 661.3 0 46 236 66 661.3 0 66 237 86 661.3 0 86 238 88 661.3 0 88 239 83 661.3 0 83 240 109 661.3 0 109 241 85 661.3 0 85 242 64 661.3 0 64 243 89 661.3 0 89 244 73 661.3 0 73 245 59 661.3 0 59 246 57 661.3 0 57 247 49 661.3 0 49 248 112 661.3 0 112 249 116 661.3 0 116 250 210 661.3 0 210 251 72 661.3 0 72 252 46 661.3 0 46 253 67 661.3 0 67 254 95 661.3 0 95 255 102 661.3 0 102 256 89 661.3 0 89 257 104 661.3 0 104 258 68 661.3 0 68 259 86 661.3 0 86 260 142 661.3 0 142 261 104 661.3 0 104 262 197 661.3 0 197 263 168 661.3 0 168 264 154 661.3 0 154 265 89 661.3 0 89 266 95 661.3 0 95 267 63 661.3 0 63 268 88 661.3 0 88 269 182 661.3 0 182 270 141 661.3 0 141 271 64 661.3 0 64 272 59 661.3 0 59 273 101 661.3 0 101 274 88 661.3 0 88 275 76 661.3 0 76 276 149 661.3 0 149 277 85 661.3 0 85 278 92 661.3 0 92 279 71 661.3 0 71 280 101 661.3 0 101 281 169 661.3 0 169 282 260 661.3 0 260 283 76 661.3 0 76 284 72 661.3 0 72 285 106 661.3 0 106 286 49 661.3 0 49 287 66 661.3 0 66 288 128 661.3 0 128 289 310 661.3 0 310 290 156 661.3 0 156 291 141 661.3 0 141 292 89 661.3 0 89 293 78 661.3 0 78 294 144 661.3 0 144 295 137 661.3 0 137 296 163 661.3 0 163 297 80 661.3 0 80 298 98 661.3 0 98 299 73 661.3 0 73 300 82 661.3 0 82 301 41 661.3 0 41 Finished in 4.37 s (50 us/read; 1.20 M reads/minute). === Summary === Total reads processed: 87,212 Reads with adapters: 7,738 (8.9%) Reads that were too short: 8 (0.0%) Reads written (passing filters): 7,730 (8.9%) Total basepairs processed: 22,909,641 bp Total written (filtered): 1,920,143 bp (8.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 7738 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 1 2 18 6 0.0 3 0 0 3 1 2 19 68 0.0 3 0 67 1 20 5817 0.0 4 5746 64 6 1 21 12 0.0 4 3 7 0 2 22 2 0.0 4 0 0 1 0 1 23 2 0.0 4 0 0 0 2 24 1 0.0 4 0 0 0 0 1 41 1 0.0 4 1 57 1 0.0 4 1 69 1 0.0 4 0 0 0 0 1 78 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 125 4 0.0 4 0 0 0 0 4 126 27 0.0 4 0 0 0 0 27 127 1779 0.0 4 0 0 0 0 1779 128 6 0.0 4 0 0 0 0 6 129 1 0.0 4 0 0 0 0 1 209 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.25 s (32 us/read; 1.86 M reads/minute). === Summary === Total reads processed: 7,730 Reads with adapters: 39 (0.5%) Reads written (passing filters): 7,730 (100.0%) Total basepairs processed: 1,920,143 bp Total written (filtered): 1,917,245 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 39 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 17.9% C: 5.1% G: 76.9% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 3 120.8 0 3 10 1 0.0 1 0 1 11 23 0.0 1 0 23 12 2 0.0 1 0 2 225 1 0.0 1 1 260 2 0.0 1 2 261 1 0.0 1 1 263 4 0.0 1 4 272 2 0.0 1 2